• BMB Reports
• /
• v.38 no.5
• /
• pp.526-532
• /
• 2005

A lectin with in-vitro anticancer activity against established human cancer cell lines has been purified by affinity chromatography on asialofetuin-linked amino activated silica beads from the tubers of Arisaema tortuosum, popularly known as Himalayan Cobra lily, a monocot plant from the family Araceae. The bound Arisaema tortuosum lectin (ATL) was eluted with glycine-HCl buffer, pH 2.5. ATL was effectively inhibited by asialofetuin, a complex desialylated serum glycoprotein as well as by N-acetyl-D-lactosamine, a disaccharide. It gave a single band corresponding to a subunit molecular weight of 13.5 kDa in SDS-PAGE, pH 8.8 both under reducing and non reducing conditions. When subjected to gel-filtration on Biogel P-200, it was found to have a molecular weight of 54 kDa, suggesting a homotetramer structure, in which individual polypeptides are not bound to each other with disulfide bonds. ATL is a glycoprotein with 0.9% carbohydrate content, stable up to $55^{\circ}C$ and at pH 2 to 10. The lectin had no requirement for divalent metal ions i.e. $Ca^{2+}$ and $Mn^{2+}$ for its activity. However, as reported for other monocot lectins, ATL gave multiple bands in isoelectric focusing and Native PAGE, pH 8.3. The lectin was found to inhibit in vitro proliferation of human cancer cell lines HT29, SiHa and OVCAR-5.

• Proceedings of the KSR Conference
• /
• 2008.06a
• /
• pp.332-338
• /
• 2008

This paper handles, implementation of multiple access bidirectional serial communications protocol using two common DC power lines, whict are power supply and ground, connecting multiple devices. Communication between the host and the multiple clients are performed using unique packet data with device ID unique to each devices connected on the common power lines. Host initiates data communications by transmitting command packet to the designated client with the client's ID and the client responds by transmitting response packet to the host and in this way, multiple clients and host exchange the packet through the common power lines. The normal voltage of the power communication line maintains 24V corresponding to level 1 and the host drops the voltage to 12V on sending level 0 signal, also the clients normally keeps the line voltage to 24V use pull-down circuit to drop the voltage to 12V on sending level 1 signal. Power supply originates from the host, the host senses the voltage level of the power communication lines and when the clients activates pull down circuit to send level 0 signal and the voltage drops to 12V, the hosts switches power source from 24V to 12V. Also, when clients deactivate pull down circuit to send level 1 signal, the host senses the voltage increase and switches the power source from 12V to 24V. Experimental circuit is designed with one hosts and four clients and verified the power line voltage switching operation depending on the data signal levels on the power line. The proposed research result can be applied to two wire power communications system with one host and multiple low current consumption clients.

• Genomics & Informatics
• /
• v.19 no.2
• /
• pp.17.1-17.13
• /
• 2021

Breast cancer is one of the leading causes of cancer in women all over the world and accounts for ~25% of newly observed cancers in women. Epigenetic modifications influence differential expression of genes through non-coding RNA and play a crucial role in cancer regulation. In the present study, epigenetic regulation of gene expression by in-silico analysis of histone modifications using chromatin immunoprecipitation sequencing (ChIP-Seq) has been carried out. Histone modification data of H3K4me3 from one normal-like and four breast cancer cell lines were used to predict miRNA expression at the promoter level. Predicted miRNA promoters (based on ChIP-Seq) were used as a probe to identify gene targets. Five triple-negative breast cancer (TNBC)-specific miRNAs (miR153-1, miR4767, miR4487, miR6720, and miR-LET7I) were identified and corresponding 13 gene targets were predicted. Eight miRNA promoter peaks were predicted to be differentially expressed in at least three breast cancer cell lines (miR4512, miR6791, miR330, miR3180-3, miR6080, miR5787, miR6733, and miR3613). A total of 44 gene targets were identified based on the 3'-untranslated regions of downregulated mRNA genes that contain putative binding targets to these eight miRNAs. These include 17 and 15 genes in luminal-A type and TNBC respectively, that have been reported to be associated with breast cancer regulation. Of the remaining 12 genes, seven (A4GALT, C2ORF74, HRCT1, ZC4H2, ZNF512, ZNF655, and ZNF608) show similar relative expression profiles in large patient samples and other breast cancer cell lines thereby giving insight into predicted role of H3K4me3 mediated gene regulation via the miRNA-mRNA axis.

• International Journal of Concrete Structures and Materials
• /
• v.2 no.1
• /
• pp.57-62
• /
• 2008

When the yield line theory is used to estimate the ultimate strength of a concrete barrier, it is of primary importance that the correct assumption is made for the failure mode of the barrier. In this study, a static test was performed on two full-scale concrete barrier specimens of Korean standard shape that simulate the actual behavior of a longitudinally continuous barrier. This was conducted in order to verify the failure mode presented in the AASHTO LRFD specification. The resulting shape of the yield lines differed from that presented in AASHTO when subjected to an equivalent crash load. Furthermore, the ultimate strengths of the specimens were lower than the theoretical prediction. The main causes of these differences can be attributed to the characteristics of the barrier shape and to a number of limitations associated with the classical yield line theory. Therefore, a revised failure mode with corresponding prediction equations of the strength were proposed based on the yield lines observed in the test. As a result, a strength that was more comparable to that of the test could be obtained. The proposed procedure can be used to establish more realistic test levels for barriers that have a similar shape.

• The Bulletin of The Korean Astronomical Society
• /
• v.40 no.2
• /
• pp.35.4-36
• /
• 2015

Gaseous inner disks are the main controller of the final structure of planetary systems as well as the building place of planets, especially of terrestrial planets. However, the inner disk of <5AU is still difficult to be spatially resolved even at the closest star forming regions. Resolving velocity structure in the disk with high resolution infrared spectroscopic study is the best approach to study the inner disk at this moment. Here, we present the IGRINS (Immersion GRating INfrared Spectrometer) result of the Class I young stellar object, ESO Ha 279a, in the Serpens molecular cloud region. IGRINS has a resolving power of R=40,000, corresponding to the velocity resolution of 7 km/s at K-band, which is perfect to study the hot inner disk structure. We report that NaI and CO overtone emission lines are indeed good tracers of the rotating inner warm disk tracing from ~0.04 to ~7AU of this source. We also report the disk properties using other emission lines.

• Bulletin of the Korean Chemical Society
• /
• v.23 no.2
• /
• pp.195-200
• /
• 2002

Fluorescence excitation spectrum of the trans.trans-1,3,5,7-octatetraene(OT)-Xe van der Waals clusters formed in supersonic jet expansions has been obtained. The transition lines corresponding to the van der Waals cluters of OT with Xe are observed in the lower frequency side of the OT band origin. Based on the spectral shifts, fluorescence lifetimes, and concentration dependence of the peak intensities, most of the transition lines are assigned to the $OT-Xe_n$ (n = 1, 2, 3, 4) clusters. Long progressions of a van der Waals vibrational mode are observed for n = 1, 2, 3 and 4 clusters and assigned to rocking of the OT moiety with respect to the Xe atom with the help of ab initio quantum mechanical calculation.

• Journal of Plant Biotechnology
• /
• v.4 no.4
• /
• pp.155-161
• /
• 2002

The HCV gene was expressed in potato plants under the control of the constitutive CaMV 355 promoter or tuber-specific patatin promoter. Solanum tuberosum plants carrying a plant expression vector harboring the encoding region of HCV gene were generated by Agrobacterium tumefaciens-mediated in vitro transformation methods. The presence of HCV gene in the plant genome was detected by PCR and DNA hybridization experiments. We obtained the 5 lines of transgenic potato with the pMBPHCV construct and 4 lines of transgenic potato with the pATHCV construct. The HCV transgenic stably integrated into the potato genome, as well as their transcription. HCV mRNA was identified in leaf and tuber tissues of transgenic plants by Northern blot analysis. The transgenic potato plants produced the expected transcript, and the corresponding HCV protein accumulated in individual transgenic plants.

• JSTS:Journal of Semiconductor Technology and Science
• /
• v.10 no.3
• /
• pp.232-239
• /
• 2010

By using the clock timing control at transmitter (TX), the crosstalk-induced jitter (CIJ) is compensated for in the 2-bit parallel data transmission through the coupled microstrip lines on printed circuit board (PCB). Compared to the authors' prior work, the delay block circuit is simplified by combining a delay block with a minimal number of stages and a 3-to-1 multiplexer. The delay block generates three clock signals with different delays corresponding to the channel delay of three different signal modes. The 3-to-1 multiplexer selects one of the three clock signals for TX timing depending on the signal mode. The TX is implemented by using a $0.18\;{\mu}m$ CMOS process. The measurement shows that the TX reduces the RX jitters by about 38 ps at the data rates from 2.6 Gbps to 3.8 Gbps. Compared to the authors' prior work, the amount of RX Jitter reduction increases from 28 ps to 38 ps by using the improved implementation.

• Structural Engineering and Mechanics
• /
• v.76 no.2
• /
• pp.207-222
• /
• 2020

Due to the rugged terrain, metro lines in mountain city across numerous wide rivers and deep valleys, resulting in instability of high-pier bridge and insecurity of metro train subjected to fluctuating crosswind. To ensure the safe operation in metro lines in mountain cities, running safety of the metro train over the high-pier bridge under crosswind is analyzed in this paper. Firstly, the dynamic model of the wind-train-bridge (WTB) system is built, in which the speed-up effect of crosswind is fully considered. On the basis of time domain analysis, the basic characteristics of the WTB system with high-pier are analyzed. Afterwards, the dynamic responses varies with train speed and wind speed are calculated, and the safety zone of metro train over a high-pier bridge subjected to fluctuating crosswind in mountain city is determined. The results indicate that, fluctuating crosswind triggers drastic vibration to the metro train and high-pier bridges, which in turn causes running instability of the train. For this reason, the corresponding safety zone for metro train running on the high-pier is proposed, and the metro traffic on the high-pier bridge should be closed as the mean wind speed of standard height reaches 9 m/s (15.6 m/s for the train).

• Asian Pacific Journal of Cancer Prevention
• /
• v.17 no.11
• /
• pp.4853-4856
• /
• 2016

Objective: Gimatecan is a new camptothecin (CPT) analogue that inhibits tumor growth by targeting DNA topoisomerase I (TOP I) and introducing strong and persistent DNA cleavage. Anti-tumor activity has been demonstrated with a wide range of solid tumors in previous preclinical and clinical studies. Here, we investigated for the first time the effects of gimatecan on the proliferation of hepatocellular carcinoma (HCC) cells both in vitro and in vivo. Methods: Anticancer efficacy of gimatecan were evaluated in a panel of HCC cell lines and corresponding mouse xenograft models. Inhibition of cell proliferation was measured by CellTiter-Glo cell viability assay. In vivo, gimatecan and control preparations were orally administered every four days, for a total of four times. Tumor volume and body weights of the mice were measured twice weekly. Results: In vitro cytotoxicity evaluation showed that gimatecan inhibited the proliferation of a large panel of HCC cell lines in a dose dependent manner, with IC50 values ranging between 12.1~1085.0 nM. In vivo evaluation in mouse xenograft models showed significant antitumor effects of gimatecan at 0.8mg/kg and 0.4mg/kg as compared to the control group. Conclusion: This study suggested that gimatecan may have the potential to be used as a chemotherapeutic agent for the treatment of HCC.