• Title/Summary/Keyword: Corioius versicolor

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Production and Properties of Laccase from Coriolus versicolor (Coriolus versicolor에 의한 Laccase 생산(生産) 및 성질(性質)에 관한 연구(硏究))

  • Hong, Jai-Sik;Kim, Myung-Kon;Kim, Yun-Hi;Lee, Jong-Bae
    • The Korean Journal of Mycology
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    • v.15 no.2
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    • pp.99-170
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    • 1987
  • The production and properties of laccase(E.C.1.10.3.2) from Coriolus versicolor were studied. The results were as follows; The nutritional optimum conditions for laccase production were 1% indulin At, 0.3% peptone 0.1% $KH_2PO_4$, 0.02% $MgSO_4$, 0.1 mg% $CuSO_4$.and 0.005 mg% thiamine HCI. The optimum temperature and pH of laccase production were $25^{\circ}C$ and 5.0, and respectively, and the cultural period was 20 days. The optimum pH and temperature for the activity were 4.6 and $40^{\circ}C$, respectively. The enzyme was almost stable under the temperature of $40^{\circ}C$ and within the pH range of 4.0-5.0. The enzyme was stable at $40^{\circ}C$ for 30 min. $Cu^{++}$, $Fe^{++}$ and $Ca^{++}$ activated the enzyme activity, but $Mn^{++}$ and $Hg^{++}$ were inhibited. The enzyme was totally inhibited by 1 mM sodium azide and 1 mM potassium cyanide, and partly inhibited by EDTA and hydroxyamine.

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