• 응용통계연구
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• 제24권5호
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• pp.847-859
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• 2011

cDNA microarray-based comparative genomic hybridization(CGH) data includes low-intensity spots and thus a statistical strategy is needed to detect subtle differences between different cancer classes. In this study, genes displaying a high frequency of alteration in one of the different classes were selected among the pre-selected genes that show relatively large variations between genes compared to total variations. Utilizing copy-number changes of the selected genes, this study suggests a statistical approach to predict patients' classes with increased performance by pre-classifying patients with similar genetic alteration scores. Two-stage logistic regression model(TLRM) was suggested to pre-classify homogeneous patients and predict patients' classes for cancer prediction; a decision tree(DT) was combined with logistic regression on the set of informative genes. TLRM was constructed in cDNA microarray-based CGH data from the Cancer Metastasis Research Center(CMRC) at Yonsei University; it predicted the patients' clinical diagnoses with perfect matches (except for one patient among the high-risk and low-risk classified patients where the performance of predictions is critical due to the high sensitivity and specificity requirements for clinical treatments. Accuracy validated by leave-one-out cross-validation(LOOCV) was 83.3% while other classification methods of CART and DT performed as comparisons showed worse performances than TLRM.

• 펄프종이기술
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• 제40권2호
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• pp.73-79
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• 2008

The permanence is of great importance to library and archival paper. According to the established standardizations including ISO and ANSI, the permanence can be specified with tear strength, pH, alkali reserve and kappa number. In this study, we evaluated the permanence and durability of domestic coated and copy paper grades. Two types of coated paper grades and all kinds of copy papers which were tested in this study were satisfied with the criterion of permanence specification. However, all of the tested paper grades didn't meet the requirements of durability because of their low folding endurance.

• Journal of Communications and Networks
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• 제9권1호
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• pp.43-49
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• 2007

The implementation of client polling as a weak cache coherence mechanism has two major drawbacks: Firstly, the cache may return a stale copy if the object is changed in the origin server while the cached copy is considered valid. Secondly, the cache can invalidate a cached copy that is still valid in the server. Therefore, we propose a multilevel pre-fetching (MLP) in conjunction with the client polling to refine these drawbacks. MLP is introduced to improve the level of freshness among the cached objects. The simulation results presented in this paper show that the proposed MLP significantly minimizes the number of stale objects and reduces the invalidation messages sent out to the server, i.e., increase the cache HIT rate.

• 한국인터넷방송통신학회논문지
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• 제16권4호
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• pp.73-80
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• 2016

인터넷을 이용하는 멀티플레이어 온라인 게임(MOG)은 전형적으로 클라이언트-서버 또는 peer-to-peer 구조를 기반으로 구성된다. 본 논문에서는 두 가지 구조의 장점을 활용하기 위하여 클라이언트-서버 구조와 peer-to-peer 구조를 결합하는 방법을 제시한다. 대부분의 멀티플레이어 온라인 게임들은 객체마다 강력한 일관성 제어를 제공하고자 기본 사본을 갖는 복제 기법을 사용한다. 이 기법에서 각 객체와 캐릭터는 기본 사본이라고 하는 절대적 사본과 2차 사본이라고 하는 복제본들로 구성된다. 객체에 대한 갱신은 기본 사본에서 먼저 수행되어야만 한다. 제시된 하이브리드 구조에서는 기본 사본들이 서버 또는 클라이언트에 존재할 수 있다. 이러한 구조에서는 서버가 유지하는 객체의 수를 감소시킴으로써 서버와 클라이언트들 간의 부하 조정이 가능하다. 게임은 일관성 요건이 상이한 다양한 액션의 유형들로 구성된다. 게임 일관성에 대해서는 성능과 일관성 간에 적절한 상충 관계를 제공할 수 있는 여러 수준의 기법이 합리적이다. 본 논문에서 기본 사본 모델을 갖는 하이브리드 게임 구조에 대한 성능 분석 결과가 기술된다.

• Fisheries and Aquatic Sciences
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• 제18권1호
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• pp.73-80
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• 2015

To examine the interrelationship between transgenic insertion patterns and transgene expression profiles in established transgenic fish lines, four stable transgenic marine medaka Oryzias dancena germlines harboring ${\beta}$-actin regulator-driven RFP reporter constructs were selected. The established transgenic strains were characterized with regard to their transgenic genotypes (insertion pattern, concatemer formation, and transgene copy number based on genomic Southern blot hybridization and qPCR assay) and expression characteristics at the mRNA (qRT-PCR), protein (western blot), and phenotypic (fluorescent appearance) levels. From comparative examinations, it was found that transgenic expression at both the transcription and translation levels could be significantly downregulated in transgenic strains, potentially through methylation-mediated transgene silencing that was particularly associated with the formation of a long tail-to-head tandem concatemer in the chromosomal integration site(s). When this occurred, an inverse relationship between the transgene copy number and fluorescence intensity was observed in the resultant transgenic fish. However, with the other transgenic genotype, transgenic individuals with an identical Southern blot hybridization pattern, containing a tandem concatemer(s), had very different expression levels (highly robust vs. low expression strengths), which was possibly related to the differential epigenetic modifications and/or degrees of methylation. The concatemer-dependent downregulation of transgene activity could be induced in transgenic fish, but the overall pattern was strain-specific. Our data suggest that neither a low (or single) transgene copy number nor tandem transgene concatemerization is indicative of strong or silenced transgene expression in transgenic fish carrying a ubiquitous transgene. Hence, a sufficient number of transgenic lineages, with different genotypes, should be considered to ensure the establishment of the best-performance transgenic line(s) for practical applications.

• 한국멀티미디어학회논문지
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• 제17권9호
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• pp.1070-1075
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• 2014

Due to a number of illegal copied videos, many video content markets have been threatened. Since this copied videos have intercepted the profits of the content holders, content developers lose the will to generate new contents. Therefore, video copy detection approaches have been developed to protect the copyrights of video contents. However, many illegal uploader who generate copied videos used video transformations to avoid video copy detection systems. Among of the video transformations, rotation and flipping did not distorted the quality of video contents. Thus, these two video transformations were adopt to generate copied video. In order to detect rotated or flipping copy videos, rotation and flipping robust region binary pattern (RFR) recently was proposed. But, this RFR has a weakness according to rotated angles. Therefore, in order to overcome this problem, multi region binary patterns are proposed in this paper. The proposed method has the similar performance with the original RFR. But, it showed much higher efficiency for memory spaces.

• 미생물학회지
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• 제29권2호
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• pp.80-84
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• 1991

We cloned and expressed hepatitis B viral core antigen (HBcAg) gene in E. coli using $P_{L}$ promoter system. For optimal expression of the gene, we undertook the studies on the effects of the distance between Shine-Dalgarno (SD) sequence and start codon, copy number of repressor gene, induction temperature, and the stability of the core antigen. The results demonstrated that the induction at 37.deg.C was more efficient than at 42.deg.C, and the 11 base pairs (bp) distance between SD sequence and start codon of HBcAg gene was more efficient than the 15 bp distance in E. coli. The copy number of cI857 repressor gene did not influence on the expression of HBcAg, and the expression level of HBcAg in mutant type (low protease activity) and wild type strains was almost the same. The produced core antigen appeared to be HBcAg not HBeAg judged by two different radioimmunoassat (RIA) kits. This result suggested that the antigen was stable in E. coli.i.

• 한국미생물·생명공학회지
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• 제23권5호
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• pp.544-549
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• 1995

The alkaline elastase is an extracellular serine protease of the alkalophilic Bacillus strain Ya-B. To increase the gene copy number and the production level of the alkaline elastase Ya-B, we designed, on the B. subtilis chromosome, a gene amplification of the 10.6 kb repeating unit containing amyE, aleE (alkaline elastase Ya-B gene) and tmrB. The aleE was inserted between amyE and tmrB, and B. subtilis APT119 strain was transformed with this amyE-aleE-tmrB-junction region fragment. As a result, we succeeded in obtaining tunicamycin-resistant (Tm$^{r}$) transformants (Tf-1, Tf-2) in which the designed gene amplification of 10.6 kb occurred in chromosome. The transformants showed high productivity of $\alpha $-amylase and alkaline elastase Ya-B. The copy number of the repeating unit (amyE-aleE-tmrB) was estimated to be 25, but plasmid vector (pUC19) was not integrated. The amplified aleE of chromosome was more stable than that of plasmid in absence of antibiotics.

• Journal of Communications and Networks
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• 제5권3호
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• pp.222-229
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• 2003

In this paper, we propose a delayed multiple copy retransmission (DMCR) scheme for data communication in wireless networks, by which multiple copies of a lost link layer frame are retransmitted one-by-one with a retransmission delay in between. The number of the copies gradually increases with the number of retransmissions. Furthermore, for implementation of the DMCR scheme in practical mobile communication system, we also propose a dynamic retransmission scheme by interleaving and a new round-robin scheduling algorithm. We compare our scheme with the previous non-delayed retransmission schemes on the performance of frame loss probability, channel capacity and total transmission time. Numerical results show that the DMCR scheme can achieve higher performance. The effect of the delay time on endto-end TCP throughput is investigated as well.

• 한국생물정보학회:학술대회논문집
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• 한국생물정보시스템생물학회 2006년도 Principles and Practice of Microarray for Biomedical Researchers
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• pp.125-126
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• 2006

NimbleGen has developed strategies to use its high-density oligonucleotide microarray platform (385,000 probes per array) to map both promoter binding sites and copy number variation at very high-resolution in the human genome. Here we describe a genome-wide map of active promoters determined by experimentally locating the sites of transcription imitation complex binding throughout the human genome using microarrays combined with chromatin immunoprecipitation. This map defines 10,567 active promoters corresponding to 6,763 known genes and at least 1,196 un-annotated transcriptional units. Microarray-based comparative genomic hybridisation (CGH) is animportant research tool for investigating chromosomal aberrations frequently associated with complex diseases such as cancer, neuropsychiatric disorders, and congenital developmental disorders. NimbleGen array CGH is an ultra-high resolution (0.5-50 Kb) oligo array platform that can be used to detect amplifications and deletions and map the associated breakpoints on the whole-genome level or with custom fine-tiling arrays. For whole-genome array CGH, probes are tiled through genic and intergenic regions with a median probe spacing of 6 Kb, which provides a comprehensive, unbiased analysis of the genome.