• Title/Summary/Keyword: Coprinus cinereus

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Recycling of Waste Paper with Alkaline Cellulolytic Enzymes(I) - Preparation and characteristics of cullulolytic enzymes- (호알카리성 목질분해 효소를 이용한 폐지재생(제1보) -목질분해 효소의 단리 및 특성-)

  • 강석현;이중명;박성배;엄태진
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.35 no.3
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    • pp.66-73
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    • 2003
  • Alkaline cellulolytic enzymes are prepared from Coprinus cinereus 2249. Recovery method of enzyme protein from cultured medium and effect factors on enzyme activity of protein were investigated. The results could summarized as follows, \circled1 Amount of enzyme protein from cultured medium was highest in incubation with shaking and addition of skim milk. \circled2 Protein from cultured medium was alkaline enzymatic protein which shows the highest activity at pH 9.0. \circled3 The most effective recovery method of enzyme protein was the precipitation of protein by addition of cultured medium of protein in ethanol. \circled4 The enzyme activity was enhanced by tween-80 and decreased with $Al_2(SO_4)_3$, $H_2O_2$et al, and was little changed with metal ions except $Hg^{++}$.

Recycling of Waste Paper with Alkaline Cellulolytic Enzyme(III) - Elimination of sticky contaminants and bleaching of recycling pulp- (호알칼리성 목질 분해 효소를 이용한 페지 재생(제3보) -페지의 이물질 제거 및 표백-)

  • 강석현;이중명;박성배;엄태진
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.36 no.1
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    • pp.30-36
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    • 2004
  • Waste papers(MOW and ONP) were deinked with alkaline cellulolytic enzyme from Coprinus cinereus 2249. The effectiveness of alkaline enzyme on sticky contaminant removal and bleaching of recycling pulp was investigated. The conclusions obtained from the results are as follows. The brightness of deinked pulps MOW and ONP was most high in 0.4IU enzyme treatment. \circled2 The removal effect of sticky contaminant was enhanced with alkaline cellulolytic enzyme treatment. Bleaching with sodium hydrosulfite was most effective in one and two stage bleaching after enzyme treatment of mixed wastepaper of MOW and colored paper. \circled4 The brightness of alkaline enzyme deinked ONP was increased 20% with $H_2O$$_2$ bleaching.

RECYCLING OF WASTEPAPER WITH ALKALINE ENZYME FROM COPRINACEAE SP.

  • Eom, Tae-Jin;Lee, Jung-Myoung
    • Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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    • 1999.04b
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    • pp.291-295
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    • 1999
  • Coprinus cinereus 2249 that is a kind of basidiomycetes constitutively produced alkaline carboxymethyl cellulase (CMCase), filter paper cellulase (FPase) and xylanase. Crude enzymes prepared with optimal conditions showed higher FPase activity than CMCase activity. The FPase was most active at pH 9 at 50$^{\circ}C$. When applied on deinking of the old newsprint (ONP), it increases the freeness and brightness due to effect of hydrolysis at 0.1% enzyme concentration. Also, The physical properties of deinked pulp were improved.