• Mycobiology
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• 제38권4호
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• pp.249-255
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• 2010

We identified Lacrymaria velutina of the Coprinaceae in Korea. The unusually large and sturdy fruiting body, fibrillose to fibrillose-scaly cap and stalk without a volva with an obscure superior hairy ring zone or hairy annulus, and blackish brown, warted spores distinguished this species from closely related Psathyrella species. An illustrated account of the microscopic traits is presented. Fruiting bodies with obtusely hemispherical caps, 2.5~6 cm, becoming convex with age; surface dry, densely fibrillose-scaly with split margin; stipe, 4.5~6 cm, equal, hollow, fibrillose, dry, whitish above the superior ring zone, light brown below; crowded gills, adnexed, dark black at maturity. Pileipellis typically cellular with the gill edge appearing white and beaded. Blackish brown basidiospores that discolor in concentrated sulfuric acid. Spores elliptical, warted, $9\sim11{\times}6\sim8{\mu}m$, with prominent snout-like germpores. Cheilocystidia abundant, $57\sim68{\times}19\sim25{\mu}m$, and narrowly elongated clavate, often clustered in threes or fours. Pleurocystidia rarely present, $45\sim47.5{\times}12\sim13{\mu}m$, and clavate to utriform. This trait distinguishes our sample as L. velutina from other Psathyrella spp. of the Coprinaceae, which have smooth spores. This taxon was clarified by the observation that Psathyrella spores fade in concentrated sulfuric acid. A molecular phylogenetic study revealed that our specimen was Lacrymria velutipes, which is closely related to Lacrymaria lacrymabunda. Moreover, those two species are clearly distinguishable from other Psathyrella species, which agreed with the morphologically distinctive traits described above. We believe that this is the first report of this taxon, which has not been described in Korea.

• 펄프종이기술
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• 제31권3호
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• pp.68-76
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• 1999

Coprinus cinereus 2249 producing alkaline-active cellulase was screened from 29 species of Corpinaceae and constitutively produced alkaline carboxymethyl cellulase (CMCase) and filter paper cellulase (Fpase). When cultivated at pH 9.0, 25$^{\circ}C$ and 5 days, copnnus cinereus 2249 produced higher alkaline activity on 0.5% CMC, 2% wheat bran as carbon source and 0.5% peptone, 0.05% yeast extract as nitrongen source compared with other culture conditions. The level of cellulase production was higher in the presence of wheat bran than in the presence of CMC. The optimum temperature and pH for alkaline -active cellulase activity weas 50$^{\circ}C$ and 9, 0, respectively.

• 한국펄프종이공학회:학술대회논문집
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• 한국펄프종이공학회 1999년도 Pre-symposium of the 10th ISWPC Recent Advances in Paper Science and Technology
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• pp.291-295
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• 1999

Coprinus cinereus 2249 that is a kind of basidiomycetes constitutively produced alkaline carboxymethyl cellulase (CMCase), filter paper cellulase (FPase) and xylanase. Crude enzymes prepared with optimal conditions showed higher FPase activity than CMCase activity. The FPase was most active at pH 9 at 50$^{\circ}C$. When applied on deinking of the old newsprint (ONP), it increases the freeness and brightness due to effect of hydrolysis at 0.1% enzyme concentration. Also, The physical properties of deinked pulp were improved.

• 한국균학회지
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• 제36권1호
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• pp.51-57
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• 2008

식용과 약용버섯으로서 이용가치가 높은 것으로 알려진 먹물버섯(C. comatus)에 대하여 보다 우수한 인공재배법을 개발하고 자실체의 형태적 특성에 대한 연구 결과는 다음과 같다. 먹물버섯속균 5개 균주 중에서 C. comatus균인 CM 980301 균주를 우량균주로 선발하였으며, 먹물버섯(C. comatus: CM 980301)자실체를 생산하기 위한 우수 배지는 종균용으로 밀곡립배지를 사용하고, 먹물버섯을 볏짚 발효배지에 인공재배 하였을 때 $24{\sim}28^{\circ}C$에서 균배양은 종균 접종 후 15일, 복토 후 13일이 소요되었으며, 원기형성 및 초발이는 $20{\sim}24^{\circ}C$에서 $10{\sim}11$일이 소요되었고, 버섯생육은 $20{\sim}24^{\circ}C$에서 $7{\sim}8$일이 소요되었다. 먹물버섯 (CM 980301)의 수확적기는 갓이 피기 전으로 자실체 경도는 갓 $102{\sim}169\;g/cm^2$, 대 $126{\sim}182\;g/cm^2$으로 단단하였고, 인공재배한 수량성은 $37.7kg/3.3m^2$, 개체중 17.9 g이었다.