• Journal of Veterinary Science
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• v.22 no.4
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• pp.56.1-56.10
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• 2021

Background: Fluorescent antibody virus neutralization (FAVN) test is a standard assay for quantifying rabies virus-neutralizing antibody (VNA) in serum. However, a safer rabies virus (RABV) should be used in the FAVN assay. There is a need for a new method that is economical and time-saving by eliminating the immunostaining step. Objectives: We aimed to improve the traditional FAVN method by rescuing and characterizing a new recombinant RABV expressing green fluorescent protein (GFP). Methods: A new recombinant RABV expressing GFP designated as ERAGS-GFP was rescued using a reverse genetic system. Immuno-fluorescence assay, peroxidase-linked assay, electron microscopy and reverse transcription polymerase chain reaction were performed to confirm the recombinant ERAGS-GFP virus as a RABV expressing the GFP gene. The safety of ERAGS-GFP was evaluated in 4-week-old mice. The rabies VNA titers were measured and compared with conventional FAVN and FAVN-GFP tests using VERO cells. Results: The virus propagated in VERO cells was confirmed as RABV expressing GFP. The ERAGS-GFP showed the highest titer (10^8.0 TCID₅₀/mL) in VERO cells at 5 days post-inoculation, and GFP expression persisted until passage 30. The body weight of 4-week-old mice inoculated intracranially with ERAGS-GFP continued to increase and the survival rate was 100%. In 62 dog sera, the FAVN-GFP result was significantly correlated with that of conventional FAVN (r = 0.95). Conclusions: We constructed ERAGS-GFP, which could replace the challenge virus standard-11 strain used in FAVN test.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.25 no.11
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• pp.1495-1504
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• 2021

An ultra-compact binary power generation plant converts thermal energy into electric power using temperature difference between heat source and cooling source. In the actual power generation environment, the characteristic value of the plant changes due to any negative effects such as environmental condition or corrosion of related equipment. If the characteristic value of the plant changes, it may lead to unstable output of the turbine in a conventional PID control system with fixed PID parameters. A Neuro PID control system based on Neural Network adaptively to adjust the PID parameters according to the change in the characteristic value of the plant is proposed in this paper. Discrete-time transfer function models to represent the dynamic characteristics near the operating point of the investigated plant are deduced, and a design strategy of the proposed control system is described. The proposed Neuro PID control system is compared with the conventional PID control system, and its effectiveness is demonstrated through the simulation results.

• Korean Journal of Medicinal Crop Science
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• v.19 no.2
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• pp.83-89
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• 2011

This studies were conducted to investigate the growth responses and yields of Astragalus membranaceus Bunge which were grown with organic cultivation using two organic fertilizers and two green manure crops of hairy vetch and rye in 2008 and 2009. The lengths of stems and roots were not clearly different between organic and conventional cultivations. The root diameters and the branch numbers were slightly thicker and more in all treatments of organic cultivation than that of conventional cultivation in 2008, although they were not significantly different between two cultivating methods in 2009. The dry weights of shoot and root were heavier on organic cultivation than those on conventional cultivation. The T/R ratios were commonly higher in organic culture, showing more clearly in 2008 than 2009. The yields were generally higher in organic cultivation than those in conventional cultivation in 2008 although the effect did not clear on yield in 2009. The organic cultivation using of organic fertilizers and green manure crops would be new method to have safety and qualitative products. The techniques of organic cultivation for Astragalus membranaceus Bunge would be studied more on utilizing the natural organic resources.

• Korean Journal of Soil Science and Fertilizer
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• v.31 no.3
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• pp.253-258
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• 1998

To verify the suitability of microwave digestion method for analyzing macro nutrients and metals. K, Ca, Mg, Cu, Zn, and Mn contents in plant and composts were investigated comparing with conventional wet digestion method. Because it takes short time to digest metal samples by using microwave method compared with conventional wet digestion method. Digestion time for metal samples was four to six hours long for wet digestion, while one hour long for microwave digestion. Determinant coefficients of Cu, Mn, Zn, Ca, K, and Mg contents between two analyzing methods were 0.99, 0.99, 0.94. 0.97, 0.98, and 0.91, respectively. In the case of Ca and K, microwave digestion method analyzed their contents relatively low compared with wet digestion method. Meanwhile, microwave digestion method analyzed the contents for Cu, Ma, and Zn relatively high compared with wet digestion method. This study results in that microwave digestion method could be an alternative to replacing conventional wet digestion method.

• Korean journal of applied entomology
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• v.60 no.1
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• pp.105-114
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• 2021

Ground-dwelling arthropods are important components in apple orchard providing beneficial ecological services of predation and decomposition as well as herbivory. Groundcovers are managed differentially in organic and conventional apple orchards influencing ground-dwelling arthropod assemblages. We conducted 3-year studies to assess the effects of orchard management relative to weed management on the abundance and diversity of ground-dwelling arthropods using pitfall trapping. Most arthropods were classified as higher taxonomical groups and functional feeding guilds, while carabid beetles were classified into species level. Coleoptera was the dominating taxon of all ground-dwelling arthropods. Abundance of herbivores and predators was significantly higher in organic apple orchards than conventional ones. Higher abundance and diversity of carabid beetles were found in organic orchards than in conventional orchards. The abundance of Araneae, Coleoptera, or carabid beetles was negatively correlated to weed management frequency. It was found that ground-dwelling arthropods were more influenced by weed management practices than the farming systems.

• 제어로봇시스템학회:학술대회논문집
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• 1997.10a
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• pp.20-23
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• 1997

Refuse incineration plant operations involve many kinds of uncertain factors, such as the variable physical properties of refuse as fuel and the complexity of the burning phenomenon. That makes it very difficult to apply conventional control methods to the combustion control of the refuse. In this paper, an adaptive fuzzy model predictive controller is proposed for the combustion control of the refuse. And computer simulation was carried out to evaluate performance of the proposed controller.

• Proceedings of The Korean Society of Agricultural and Forest Meteorology Conference
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• 2003.09a
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• pp.50-53
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• 2003

Models have been developed to estimate leaf wetness duration (LWD) using conventional weather observations, e.g., air temperature, water vapor pressure, and wind speed, which are relatively invariant over space (Pedro and Gillespie, 1982; Gleason et al., 1994; Francl and Panigrahi, 1997).(omitted)

• Advances in Energy Research
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• v.3 no.1
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• pp.59-70
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• 2015

In India, continuous production of electricity and sweet/potable water from Solar power and desalination plant plays a major role in the industries. Particularly in Copper industry, Solar power adopts Solar field collector combined with thermal storage system and steam Boiler, Turbine & Generator (BTG) for electricity production and desalination plant adopts Reverse osmosis (RO) for sweet/potable water production which cannot be used for long hours of power generation and consistency of energy supply for industrial processes and power generation cannot be ensured. This paper presents an overview of enhanced technology for Solar power and Desalination plant for Copper industry making it continuous production of electricity and sweet/potable water. The conventional technology can be replaced with this proposed technique in the existing and upcoming industries.

• Nuclear Engineering and Technology
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• v.28 no.3
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• pp.339-348
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• 1996

Plant monitoring algorithm developments seem to be saturated which means that display system to show the results from the algorithm should be the well-defined and interactive tools for operator's diagnosing, controlling, restoring the abnormal plant situations. On the other hand, very little generalized display design concepts and evaluations are available. Events that are unfamiliar to operators and that has not been anticipated by designers may cause great threats to the nuclear power plant system safety operation. The abstraction hierarchy, considered most popular display design methodology but not generalized for nuclear power plant design space, has ken proposed as a representation frame work that can be adopted to design interfaces and supports operators in diagnosing overlooked events that should have been considered to operate plant safely. However most practical plant display systems do not fully stick to this design concept but partially rely on their philosophy from design experiences. Abstraction hierarchy display design concept will be do scribed and the trend of Advanced Control Room(ACR) CRT design will also be presented with the conventional display for the several type of plants. Consequently this complementary material should be of interest to designer and regulators concerned with nuclear power plant.

• Korean Journal of Plant Resources
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• v.29 no.6
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• pp.704-711
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• 2016

Tomatoes are considered as one of the main components of daily meals in most households. Thus, it is important to invest in studies enhancing their yield and nutritional value. The study evaluated the effect of wood vinegar (WV) on tomato under greenhouse conditions. Data on fruit number, fruit weight, and plant height were recorded. Total phenolic content (TPC) and antioxidant activity of tomato were determined using the Folin-Ciocalteu reagent and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, respectively. FRR-CF+500x-WV significantly increased the fruit number (86.11%) and fruit weight (81.78%) over the control. Results were comparable to HRR-CF+250x-WV, indicating that conventional fertilizer and WV may have synergistic effect on each other. TPC and scavenging effect on DPPH radical was improved by 250x-WV application. The increase in the phenolic compounds can be attributed to WV as a direct source or as a factor triggering the plants to produce more secondary metabolites with the purpose of increasing natural defenses. The significant effect obtained by applying the full recommended rate of conventional fertilizer on the carotenoid content was due to the availability of the major nutrients needed by the plant. Correlation analysis showed that carotenoids have negative correlation with TPC and DPPH. However, TPC and DPPH showed that these are positively correlated with each other. Tomatoes are excellent source of antioxidants associated with the reduction of some human diseases and improved health. The results provided evidence that WV alone and/or its combination with conventional fertilizers has favorable effects on the quality of tomato.