• Parasites, Hosts and Diseases
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• v.24 no.1
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• pp.1-11
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• 1986

Larval anisakids found in the yellow corvina (Pseudosciaena manchurica), a marine fish caught in the Yellow Sea, were classified by their morphological types. Total 1, 068 anisakid larvae were collected from 30 fish examined, with the average number per fish of 35.6. They were classified into Anisakis type I larvae of Berland (859 in number, 80.4%), Contracaecum type A of Koyama et at. (13, 1.2%), Contra caecum type C'(new type) (55, 5.1%), Contracaecum type D of Koyama et aZ. (18, 1.7%), Contracaecum type D' (new type) (77, 7.2%), Contracaecum type V of Yamaguti (3, 0.28%), Raphidascaris sp. of Koyama et aZ. (1, 0.09%) and unidentified (42, 3.9%). Contracaecum type C' and D' were considered new in the literature.

• Journal of Life Science
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• v.19 no.9
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• pp.1328-1332
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• 2009

Anisakidosis is caused by anisakid nematodes (family Anisakidae) larvae which can cause not only direct tissue damage but also a severe allergic response related to excretory-secretion products. Lots of different species of anisakid larvae, including Anisakis simplex, Contracaecum, Goezia, Pseudoterranova, and Hysterothylacium, cause the anisakidosis. But it is difficult to diagnosis the species of larvae since the morphologies of larval anisakid nematodes are almost indistinguishable. In order to diagnosis the differential infections of larval anisakid nematodes, polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) of 18S rDNA - was conducted. Three major species of anisakid larvae including A. simplex, C.ontracaecum spp, and Goezia spp. were collected from mackerel (Scomber japonicus), mullet (Mugil cephalus), founder (Paralichthys olivaceus), eel (Astroconger myriaster) and red sea bream (Pagrus major). PCR amplified 18S rDNA from each species of anisakid larvae was digested with eight restriction enzymes including Taq I, Hinf I, Hha I, Alu I, Dde I, Hae III, Sau96 I, and Sau3A I. The original sizes of PCR amplified 18S rDNA were 2.0Kb in both anisakid larvaes and Goezia. Restrction enzymes including Hinf 1, Alu 1, Hha I, Dde 1 and Hae III cut differently and distinguished the A. simplex and Contracaecum type C'. However, Contracaecum type A showed two different restriction enzyme cutting patterns by Taq 1, Hinf I, Alu 1, and Dde 1. One of the patterns was the same as those of A. simplex, Contracaecum type C' and Goezia and the other was unique. These results suggest that PCR-RFLP pattern by Hinf 1, Alu 1, Hae I, Dde 1 and Hae III can be applied to differential diagnosis of human infection with A. simplex and Contracaecum type C'. Contracaecum type A needs further study of classification by morphological characteristics and genetic analysis.

• Journal of Food Hygiene and Safety
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• v.17 no.4
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• pp.206-209
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• 2002

From February through March 2002, sixteen rockfishes representing three species were collected from Oregon Coast Aquarium and Newport fish market and examined far nematodes. During this study, a total of 157 Anisakis type larvae were recovered from Sebastes spp. and parasitized for an average infection prevalence of 50.0%. The yellowtail rockfish, S. flavidus, darkblotched rockfish, S. crameri, and canary rockfish, S. pinniger examined were infected with 110 Anisakis simplex larva, 30 Contracaecum spp. larva and 17 Pseudoterranova decipiens larva. Of the rockfishes examined, 75.0% carried Sebastes crameri, and 12.5% S. flavidus and S. crameri, respectively. The sites of infection far Anisakis type larvae were the intestine, omentum, and stomach walls. A. simplex larvae infected 60.0% on S. crameri and 33.3% on S.flavidus and S. pinniger respectively. The prevalence of Contracaecum sp. larvae and Pseudoterranova decipiens larvae on S. pinniger was 33.3%. The intensities of A. simplex larvae ranged from 1 to 87 in S. flavidus, S. crameri, and S. pinniger. The intensity of Contracaecum spp. larvae and P. decipiens larvae ranged from 0 to 30 in S. pinniger.

• 한국해양학회지
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• v.30 no.3
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• pp.197-202
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• 1995

A total of 272 specimens of Todarodes pacificus purchased during the period from July 1 to August 30, 1994 in the southern sea off Pusan were examined for their infection status with larval anisakids. Larvae in squids were encapsulated and appeared to remain active. Firty five larval anisakids sorted from T. pacificus (7.72% of infection rate) were classified based on morphological and morphometric observations as follows; Anisakis type I larvae (23 larvae, 51.0%: positive rate), Contracaecum type A (9, 20.0%), Contracaecum D (4, 9.0%), Anisakis II (3, 6.7%) and unknown type (6, 13.3%).

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.1 no.1
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• pp.55-57
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• 1996

A total of 248 specimens of Todarodes pacificus were purchased from the Jumungin fishery market at the East Sea area two times during the period from November 1 to December 30, 1995. Samples were examined for their infection status with larvae anisakid. Anisakid larvae were collected from muscle, viscera and omentum. One hundred sixteen larval anisakids sorted from 34 specimens of T. pacificus (13.7% of infection rate) ranged from 13.5 to 29.5 mm in their body length. They were classified based on morphological and morphometric observations as follows; Anisakis type I larvae (68 larvae, 58.6%: positive rate), Contracaecum type A (5, 4.3%), Anisakis type II (17, 14.5%), Contracaecum type D (8, 7.0%) and unknown type (18, 15.5%).

• Journal of agricultural medicine and community health
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• v.15 no.1
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• pp.3-8
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• 1990

Seven specimens of salmon(Oncorhynchus keta) purchased directly, in Oct. 10. 1990, in the Taep'o port. Kang-won-do were examined for infection state of anisakid larvae and classification of extracted larvae according to morphological characters. The results were as follows. 1) From seven salmon 202 anisakid larvae were found, and mean infection number of anisakid larvae per individual salmon was 28.86. 2) From total extracted anisakid larvae 198 larvae(98%) were found in muscle. Therefore the distribution of anisakid larvae in intestine was extremely rare compared to in muscle. 3) The percentage of anisakid larvae in II, IV muscle region was 93% and from this we could surmise that most anisakid larvae in salmon did not penetrate to the very distanted muscle from intestine. 4) Three types of anisakid larvae(Anisakis Type I, Contracaecum Type B, Contracaecum Type D) were identified and, among them, Contracaecum Type B was the first recording type in Korea 5) Larvae of Contracaecum it genus were found only in intestine. Therefore it surmised that penetration neture to muscle of Contracaecum larvae was less than that of Anisakis Type I.

• Journal of Veterinary Clinics
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• v.27 no.1
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• pp.11-16
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• 2010

A white pelican, Pelecanus onccrotalus, was imported from Tanzania and died 5 days later in Daejeon zoo in Korea. The proventriculus of bird was found to contain parasites. The taxonomic status of the nematodes was studied by morphological and morphometric analysis using light and scanning electron microscopy. Only one species Contracaecum occurred in the proventriculus with high values of infection. Morphological characters of the parasite were as follows: The worms have three lips without cuticular dentigerous ridges, and well developed interlabia. The interlabia have a triangular shape, non-bifurcated. Males possess numerous preanal papillae (69-79 pairs) arranged in single subventral rows. Paracloacal papillae were separated 5 postanal papillae and 1 larger pairs. All the characters of the above studied specimens are very similar to those of C. rudolphii complex. Accordingly, the parasites from white pelican were identified belong to the C. rudolphii complex.

• Parasites, Hosts and Diseases
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• v.30 no.3
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• pp.157-162
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• 1992

Although the sea eel (Astroconger myriaster) is suspected as one of the most important fish host for human anisakiasis in Korea, no report has been made on the infection status of the sea eel with anisakid larvae. In the present study, 26 sea eels (Astroconger myriaster) were purchased from the Noryangjin 6sh market in Seoul, and anisakid larvae were collected from their viscera, muscle, head and skin. The collected larvae were classified by their morphological types. A total of 1,351 anisakid larvae were collected from 15 of 26 fish examined. Among them, 1,269 were recovered from the viscera, 66 from the muscle, and 16 from the head and skin. Morphologically, most of the anisakids were classified into 6 known larval types, Anisakis type I (564 larvae) of Berland(1961) , Contracaecum type A(409) and type D(5) of Koyama et at. (1969), Contracaecum type C'(83) and type D'(117) of Chai et at. (1986), and Contracaecum type V(1) of Yamaguti (1935). Remaining 172 specimens were new in the available literature, hence, designated as Centracaecum type A'(new type). The present results revealed that the sea eels caught in the Korean waters are heavily infected with anisakid larvae, not only in their viscera but also in the muscle, and Anisakis type I was the most common among the 7 larval types.

• Journal of Environmental Health Sciences
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• v.23 no.1
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• pp.14-17
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• 1997

A total of 93 specimens of Astroconger myriaster were purchased from the Chungmu fishery market at the south sea area during the period from October 1 to November 25, 1996. Specimens were examined for the infection status with larvae anisakid. Anisakid larvae were collected from viscera, muscle, omenturn and liver. Eight hundred four larval anisakids sorted from 93 specimens of A. myriaster ranged from 9.0 to 26.5mm in their body length. They were classified based on morphological and morphometric observations as follows Anisakis type 1 larvae (465 larvae, 57.8%: positive rate), Contracaecum type D(129, 16.0%), Contracaecum type A(90, 11.2%), Anisakis type 11(60, 7.5%), Phapidascaris(3, 0.4%) and unknown type(57, 7.1%).

• Journal of Environmental Health Sciences
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• v.29 no.4
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• pp.17-20
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• 2003

The results of study of the parasites of rockfishes, Sebastes spp. collected at Oregon coast aquarium during April 6 to June 30, 2002 are presented. Six species of parasites were recovered from examination of 19 rockfishes with an incidence of infection of 42.1 %. This study investigated parasitic infestation of 7 black rock-fish, Sebastes melanops ; 3 quillback rockfish, S. maliger ; 2 yellowtail rockfish, S. flavidus ; 3 canary rockfish, S. pinniger 2 yelloweye rockfish, S. ruberrimus ; 2 tiger rockfish, S. nigrocinctus. Sebastes spp. from aquarium were found to be infested with parasites: Mycrocotyle sebastis, Megalocotyle trituba, Clavella uncinata, Anisakis simplex larvae, Contracaecum sp. larvae, Pseudoterranova decipiens larvae. Prevalence and mean intensity of rockfishes were infected respectively as follows: 21.1%, 4.0 of M. sebastis, 10.5%, 55.5 of M. trituba, 21.1%, 34.3 of A. simplex larvae, 10.5%, 15.0 of Contracaecum sp. larvae, 10.5%, 8.5 of Pseudoterranova decipiens larvae and 10.5%, 5.0 of Clavella uncinata. This study reveled that gills and gastrointestinal tracts of S. pinniger were heavily infested with M. trituba and A. simplex larvae.