• Journal of Digital Contents Society
• /
• v.16 no.6
• /
• pp.893-900
• /
• 2015

IMO has been proceeding in the maritime SQA for software quality is considered to be essential for the development of the introduction of the e-Navigation In order to ensure software quality, follow the prescribed procedures throughout the software development project and create the output as a result of executing the respective steps. This paper is introduced a case for applying to maritime software development using the tool that is capable of real-time monitoring and automated documentation. Also, It is discussed the improvement of procedures for applying the expected effects and maritime SQA for the tool utilization. The Development of customized tools for maritime SQA that is reflected an improved procedure for tool is the future goals.

• Biotechnology and Bioprocess Engineering:BBE
• /
• v.5 no.2
• /
• pp.84-91
• /
• 2000

Mycopasma contamination of tissue culture cells easily evades detection and, thus, represents a continous therat to cell biologists. In case where infected cell can not simply be replaced, attempts have to be made to eradicate mycoplacma from the tissue culture cells. A variety of anti-microbial agents have been shown to be toxic to mycoplasma strains ; however, cell associated mycoplasma are often protected from antibiotics at concentrations shown to be effective in vitro. Antibiotic concentrations high enough to be lethal to cell as sociated mycoplasmas frequently are also detrimentrations to the host cells, while moderately increased antibiotic levels tolerated by the host cells often lead to only temporary growth suppression and/or to the emergence of mycoplasma strains resistanct even to high concentrations of the antibiotis applied. Hare, a genetic approach for the elimination of mycoplasma from tissue culture cells that overcomes thens limitations is described. By expression of a selection marker conferring resistance to an otherwise toxic agent, Acholeplasma laidlawii infected BHK-21 cells used as the model system were enabled to temporarily tolerate antibiotic concentrations high enough to be lethal to cell associated mycopalsma while leaving the host cells unharmed. Upon successful mycoplasma eradicated, cultvation of the cured host cells in the absence of the selective agent yielded revertant cell clones that had regained susceptibillity to the toxic agent. Cressation of the selection marker expression was shown to result from the loss of the selection marker DNA, which is a consequence of the fact that the stable and permanent integration of foreign DNA in eucaryotic cell chrosomes is highly inefficient. Thus, the cells were cured from mycoplasma yet remained biochemically unaltered.