• Title/Summary/Keyword: Contiguous results

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Complete Mitochondrial Genome Sequences of Chinese Indigenous Sheep with Different Tail Types and an Analysis of Phylogenetic Evolution in Domestic Sheep

  • Fan, Hongying;Zhao, Fuping;Zhu, Caiye;Li, Fadi;Liu, Jidong;Zhang, Li;Wei, Caihong;Du, Lixin
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.5
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    • pp.631-639
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    • 2016
  • China has a long history of sheep (Ovis aries [O. aries]) breeding and an abundance of sheep genetic resources. Knowledge of the complete O. aries mitogenome should facilitate the study of the evolutionary history of the species. Therefore, the complete mitogenome of O. aries was sequenced and annotated. In order to characterize the mitogenomes of 3 Chinese sheep breeds (Altay sheep [AL], Shandong large-tailed sheep [SD], and small-tailed Hulun Buir sheep [sHL]), 19 sets of primers were employed to amplify contiguous, overlapping segments of the complete mitochondrial DNA (mtDNA) sequence of each breed. The sizes of the complete mitochondrial genomes of the sHL, AL, and SD breeds were 16,617 bp, 16,613 bp, and 16,613 bp, respectively. The mitochondrial genomes were deposited in the GenBank database with accession numbers KP702285 (AL sheep), KP981378 (SD sheep), and KP981380 (sHL sheep) respectively. The organization of the 3 analyzed sheep mitochondrial genomes was similar, with each consisting of 22 tRNA genes, 2 rRNA genes (12S rRNA and 16S rRNA), 13 protein-coding genes, and 1 control region (D-loop). The NADH dehydrogenase subunit 6 (ND6) and 8 tRNA genes were encoded on the light strand, whereas the rest of the mitochondrial genes were encoded on the heavy strand. The nucleotide skewness of the coding strands of the 3 analyzed mitogenomes was biased toward A and T. We constructed a phylogenetic tree using the complete mitogenomes of each type of sheep to allow us to understand the genetic relationships between Chinese breeds of O. aries and those developed and utilized in other countries. Our findings provide important information regarding the O. aries mitogenome and the evolutionary history of O. aries inside and outside China. In addition, our results provide a foundation for further exploration of the taxonomic status of O. aries.

The Climatology of Perceived Sultriness in South Korea (남한의 체감 무더위의 기후학)

  • 최광용;최종남;김종욱;손석우
    • Journal of the Korean Geographical Society
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    • v.37 no.4
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    • pp.385-402
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    • 2002
  • This study examined temporal and spatial patterns of bioclimatic perceived sultriness in South Korea by analyzing the climatology of heat index calculated from ambient temperature and relative humidity for the period of 26 years (1973-1998). As results, spatially, bioclimatic sultriness above all thresholds frequently occurred mainly in the central western inner area, the southwestern inner area, and the southeastern inner area of South Korea. Especially, Miryang in the southeastern inner area of South Korea was proved to be, bioclimatically, the sultriest area in South Korea that has recorded frequent high heat index. Temporally, frequency of sultriness in the contiguous South Korea significantly increased in the 1990s. Specifically, In 1994, frequencies of heat index exceeding each threshold was the highest all over South Korea. Normally, the sultriness with high heat index exceeding 40.6$^{circ}C$ occurred mainly for the period of between the end of July generally after Summer Changma and the beginning of August before another small rainy season of years. These temporal-spatial distributions of bioclimatic sultriness in South Korea showed various patterns regionally, affected by physical and anthropogenic climatic factors such as topography, latitude, altitude, water bodies or aforestration, urbanization, and industrialization as well as movements of large-scale air masses.

Temperature Prediction of Underground Working Place Using Artificial Neural Networks (인공신경망을 이용한 심부 갱내온도 예측)

  • Kim, Yun-Kwang;Kim, Jin
    • Tunnel and Underground Space
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    • v.17 no.4
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    • pp.301-310
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    • 2007
  • The prediction of temperature in the workings for the propriety examination for the development of a deep coal bed and the ventilation design is fairly important. It is quite demanding to obtain precise thermal conductivity of rock due to the variety and the complexity of the rock types contiguous to the coal bed. Therefore, to estimate the thermal conductivity corresponding to this geological situation and complex gallery conditions, a computing program which is TemPredict, is developed in this study. It employs Artificial Neural Network and calculates the climatic conditions in galleries. This advanced neural network is based upon the Back-Propagation Algorithm and composed of the input layers that are acceptant of the physical and geological factors of the coal bed and the hidden layers each of which has the 5 and 3 neurons. To verify TemPredict, the calculated result is compared with the measured one at the entrance of -300 ML 9X of Jang-sung production department, Jang-sung Coal Mine. The difference between the results calculated by TemPredict ($25.65^{\circ}C$) and measured ($25.7^{\circ}C$) is only $0.05^{\circ}C$, which is less than the allowable error 5%. The result has more than 95% of very high reliability. The temperature prediction for the main carriage gallery 9X in -425 ML under construction when it is completed is made. Its result is $28.2^{\circ}C$. In the future, it would contribute to the ventilation design for the mine and the underground structures.

Diagnosis of Spinal Arachnoid Cyst using Magnetic Resonance Imaging in a Dog (개에서 자기공명영상을 이용한 척추부 지주막 낭종의 진단)

  • Shin, Chang-ho;Kim, Young-ki;Hwang, Tae-sung;Yoon, Young-min;Jung, Dong-in;Yeon, Seong-chan;Lee, Hee-chun
    • Journal of Veterinary Clinics
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    • v.32 no.5
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    • pp.464-468
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    • 2015
  • A 6-year-old, intact male maltese was presented with hindlimb ataxia of 4 day duration. Physical and neurological examinations revealed a bright, alert, and responsive dog, with no evidence of cranial nerve deficits, conscious proprioceptive deficits. Spinal reflexes of the hind and forelimbs were normal. Patellar, cranial tibial, and withdrawal reflexes were normal. Pain could not be elicited on manipulation of the neck or palpation of the spinal column. Survey radiographs of the vertebral column were unremarkable. Computed tomography (CT) scans in the transverse plane were performed. The results of CT imaging were unremarkable. Magnetic resonance imaging (MRI) in both sagittal and transverse planes was performed. The extent of the lesion was 25 mm in length by 4 mm in thickness. The spinal cord was deviated ventrally and appreared thinner. On T1-weighted and FLAIR images, a discrete hypointense lesion dorsal to the spinal cord was observed at L1-2 which was contiguous with the subarachnoid space. On T2-weighted images, this region was hyperintense, consistent with a fluid-filled structure. The signal intensity of the cysts was equivalent to cerebrospinal fluid (CSF). Surgical treatment involving dorsal laminectomy had successful outcomes.

Cortical Network Activated by Korean Traditional Opera (Pansori): A Functional MR Study

  • Kim, Yun-Hee;Kim, Hyun-Gi;Kim, Seong-Yong;Kim, Hyoung-Ihl;Todd. B. Parrish;Hong, In-Ki;Sohn, Jin-Hun
    • Proceedings of the Korean Society for Emotion and Sensibility Conference
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    • 2000.04a
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    • pp.113-119
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    • 2000
  • The Pansori is a Korean traditional vocal music that has a unique story and melody which converts deep emotion into art. It has both verbal and emotional components. which can be coordinated by large-scale neural network. The purpose of this study is to illustrate the cortical network activated by a Korean traditional opera, Pansori, with different emotional valence using functional MRI (fMRI).Nine right-handed volunteers participated. Their mean age was 25.3 and the mean modified Edinburgh score was +90.1. Activation tasks were designed for the subjects to passively listen to the two parts of Pansories with sad or hilarious emotional valence. White noise was introduced during the control periods. Imaging was conducted on a 1.5T Siemens Vision Vision scanner. Single-shot echoplanar fMRI scans (TR/TE 3840/40 ms, flip angle 90, FOV 220, 64 x 64 matrix, 6mm thickness) were acquired in 20 contiguous slices. Imaging data were motion-corrected, coregistered, normalized, and smoothed using SPM-96 software.Bilateral posterior temporal regions were activated in both of Pansori tasks, but different asymmetry between the tasks was found. The Pansori with sad emotion showed more activation in the light superior temporal regions as well as the right inferior frontal and the orbitofrontal areas than in the right superior temporal regions as well as the right inferior frontal and the orbitofrontal areas than in the left side. In the Pansori with hilarious emotion, there was a remarkable activation in the left hemisphere especially at the posterior temporal and the temporooccipital regions as well as in the left inferior and the prefrontal areas. After subtraction between two tasks, the sad Pansori showed more activation in the right temporoparietal and the orbitofrontal areas, in contrast, the one with hilarious emotion showed more activation in the left temporal and the prefrontal areas. These results suggested that different hemispheric asymmetry and cortical areas are subserved for the processing of different emotional valences carried by the Pansories.

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Continuous Speech Recognition based on Parmetric Trajectory Segmental HMM (모수적 궤적 기반의 분절 HMM을 이용한 연속 음성 인식)

  • 윤영선;오영환
    • The Journal of the Acoustical Society of Korea
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    • v.19 no.3
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    • pp.35-44
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    • 2000
  • In this paper, we propose a new trajectory model for characterizing segmental features and their interaction based upon a general framework of hidden Markov models. Each segment, a sequence of vectors, is represented by a trajectory of observed sequences. This trajectory is obtained by applying a new design matrix which includes transitional information on contiguous frames, and is characterized as a polynomial regression function. To apply the trajectory to the segmental HMM, the frame features are replaced with the trajectory of a given segment. We also propose the likelihood of a given segment and the estimation of trajectory parameters. The obervation probability of a given segment is represented as the relation between the segment likelihood and the estimation error of the trajectories. The estimation error of a trajectory is considered as the weight of the likelihood of a given segment in a state. This weight represents the probability of how well the corresponding trajectory characterize the segment. The proposed model can be regarded as a generalization of a conventional HMM and a parametric trajectory model. The experimental results are reported on the TIMIT corpus and performance is show to improve significantly over that of the conventional HMM.

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Histopathological studies on the influence of mast cell in the growth of rat mammary carcinoma 1. Distribution of mast cell on the development of DMBA-induced mammary carcinoma (Rat mammary carcinoma의 발육(發育)에 있어서 비만세포(肥滿細胞)의 영향(影響)에 관한 병리조직학적(病理組織學的) 연구(硏究) 1. DMBA 투여(投與)에 의한 발암(發癌)과 비만세포(肥滿細胞)의 분포상황(分布狀況))

  • Kim, Tae-hwan;Lee, Cha-soo
    • Korean Journal of Veterinary Research
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    • v.30 no.4
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    • pp.447-457
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    • 1990
  • In order to observe the distribution of mast cell on the stages of the mammary carcinogenesis, the numerical changes of mast cells in the mammary tumor development in rats treated with DMBA and compound 48/80 have been investigated by the light microscope. The results observed were summarized as follows: The appearance of tumor were not observed during the whole experimental period in the rats of the control group received injection of sterile saline, but tumors appeared in 100% of the animals, the tumor induction time that represented the number of days elapsing between the 3rd DMBA administration until a first tumor became $10{\times}10mm$ in diameter was $42.5{\pm}4.7$ days and the mean number of tumor masses per rat was $3.4{\pm}1.2$ in the DMBA treated group. And the majority of the DMBA-induced mammary neoplasms were appeared cervical mammary gland and thoracic mammary gland. The histological findings of mammary carcinoma were recognized adenocarcinoma in the DMBA treated group. Mast cells were distributed within the adipose tissues and the interglandular connective tissue in the control, but found to be randomly dispersed within the tumor cell masses, in the connective tissues adjacent to the periphery of the tumor, the adipose tissues and the subcutaneous tissues contiguous to the region of tumor development in the DMBA treated group. Numerical alterations of mast cells were observed in the mammary tumors that separated into three major classes of tumors: hyperplasia, atypical hyperplasia and carcinoma. The number of mast cells were distributed in the connective tissues adjacent to the mammary gland was $45.3{\pm}3.4$ cells in the control group, but was $50.2{\pm}4.9$ cells, $126.7{\pm}10.5$ cells and $340.3{\pm}19.2$ cells according to each stages of mammary tumorigenesis in the DMBA treated group.

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Accelerating Symmetric and Asymmetric Cryptographic Algorithms with Register File Extension for Multi-words or Long-word Operation (다수 혹은 긴 워드 연산을 위한 레지스터 파일 확장을 통한 대칭 및 비대칭 암호화 알고리즘의 가속화)

  • Lee Sang-Hoon;Choi Lynn
    • Journal of the Institute of Electronics Engineers of Korea CI
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    • v.43 no.2 s.308
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    • pp.1-11
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    • 2006
  • In this paper, we propose a new register file architecture called the Register File Extension for Multi-words or Long-word Operation (RFEMLO) to accelerate both symmetric and asymmetric cryptographic algorithms. Based on the idea that most of cryptographic algorithms heavily use multi-words or long-word operations, RFEMLO allows multiple contiguous registers to be specified as a single operand. Thus, a single instruction can specify a SIMD-style multi-word operation or a long-word operation. RFEMLO can be applied to general purpose processors by adding instruction set for multi-words or long-word operands and functional units for additional instruction set. To evaluate the performance of RFEMLO, we use Simplescalar/ARM 3.0 (with gcc 2.95.2) and run detailed simulations on various symmetric and asymmetric cryptographic algorithms. By applying RFEMLO, we could get maximum 62% and 70% reductions in the total instruction count of symmetric and asymmetric cryptographic algorithms respectively. Also, performance results show that a speedup of 1.4 to 2.6 can be obtained in symmetric cryptographic algorithms and a speedup of 2.5 to 3.3 can be obtained for asymmetric cryptographic algorithms when we apply RFEMLO to a processor with an in-order pipeline. We also found that RFEMLO can effectively improve the performance of these cryptographic algorithms with much less cost compared to issue-width increase available in Superscalar implementations. Moreover, the RFEMLO can also be applied to Superscalar processor, leading to additional 83% and 138% performance gain in symmetric and asymmetric cryptographic algorithms.

Molecular Cloning of nifH, D from Frankia EuIK1 Strain, A Symbiont of Elaeagnus umbellata Root Nodules (보리수나무 뿌리혹 공생균주인 Frankia EuIK1의 nifH, D클로닝)

  • Kim, Ho-Bang;Kim, Chun-Ho;Song, Seun-Dal;An, Chung-Sun
    • Korean Journal of Microbiology
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    • v.32 no.4
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    • pp.258-263
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    • 1994
  • Genomic Southern hybridization of Frankia EuIKl strain, a nitrogen fixing symbiont of Elaeagnus umbellate root nodules, with nifH,D of K. pneumoniae as a probe, showed that 3.2 Kb and 5.5 Kb of BamHI fragments and 15 Kb PstI fragment were strongly hybridized with the probe, indicating nifH,D are located on these fragments. Using the same probe, one clone(pEuNIF) was isolated from the genomic library constructed into pWE15 cosmid vector by colony hybridization. The 3.2 Kb and 5.5 Kb BamHI fragments of this clone were hybridized with the same probe and this result corresponds to the genomic Southern hybridization data. However, using nifH of Frankia FaCl strain as a probe, only the 3.2 Kb BamHI fragment showed hybridization signal. Amino acid sequence deduced from nucleotide sequence of 3' terminus of the 3.2 Kb and 5' terminus of the 5.5 Kb fragments showed that the former was highly homologous with that of ArI3 nifD from 182nd to 240th amino acids, while the latter was from 241st to 282nd amino acids. These results show that nifH and partial nifD sequences are located on the 3.2 Kb fragment and residual sequences of nifH on the 5.5 Kb fragment which is contiguous to the 3.2 Kb fragment.

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The Ultrastructure of the Cutaneous Cells in Rana temporaria dybowskii Guenther (북방산개구리 피부 색소세포의 미세구조)

  • Kim, Han-Hwa;Chi, Young-Duk;Moon, Young-Wha
    • The Korean Journal of Zoology
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    • v.28 no.3
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    • pp.137-150
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    • 1985
  • The dorsal skin of Rana temporaria dybowskii Guenther was examined under electron microscope. The results of the fine structures in the xanthophores, iridophores and melanophores were as follows: Xanthophores: Xanthophores were filled with pterinosomes and carotenoid vesicles. Type I pterinosomes had a clear limiting membrane. Type II pterinosomes had the inner fibrous structures. Tyep III pterinosomes were characterized by a few superficial lamellae and type IV pterinosomes by multiple concentric lamellae. Especially typical type II and type III pterinosomes were evenly distributed in the cytoplasm. Iridophores: Iridophores were situated between a xanthophore and a melanophore in the outer part of the dermis just below the basement membrane. Iridophores were filled with reflective platelets, each of which is rectangular and convex lens-like in shape. These platelets were closely contiguous and leave no interspace between them. Endoplasmic reticulum and a few mitochondria were observed in the supranuclear cytoplasm. Melanophores: Dermal melanophores contained numerous melanosomes. The dendritic precesses of the melanophore containing the melanin granules extented up the lateral sides of the iridophore. Epidermal melanophores were filled with melanin granules which appered as the same electron density. A few melanin granules were observed in a cornified surface cell.

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