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The Effect of Pulse Electric Field on Accumulation of Selenium in Cells of Saccharomyces cerevisiae

  • Pankiewicz, Urszula;Jamroz, Jerzy
    • Journal of Microbiology and Biotechnology
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    • v.17 no.7
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    • pp.1139-1146
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    • 2007
  • Cultures of Saccharomyces cerevisiae were subjected to the effect of PEF (pulse electric field) and a source of selenium. The culture period after which yeast cells were subjected to PEF treatment was optimized, as was the duration of the exposure. Optimization of the nutrient medium composition in S. cerevisiae cultures resulted in an over 1.8-fold increase in selenium accumulation with relation to cultures on the initial substrate. Optimization of the pH value and of culture duration resulted in selenium accumulation increase by approximately 78%. A significant correlation was found between the accumulation of selenium in yeast cells and its concentration in the culture substrate. The highest accumulation of selenium in the biomass of yeast, approx. $240\;{\mu}g/g$ d.m., was obtained after 15-min exposure to PEF on a 20-h culture. An approx. 50% higher content of selenium in cells was recorded, as compared with the control culture without the application of PEF.

Effects of Speech Rate on the Sentence Perception of Adults with Cochlear Implantation (말속도가 인공와우 청각장애인의 문장지각에 미치는 영향)

  • Shin, Su-Jin;Shin, Ji-Cheol;Yoon, Mi-Sun;Kim, Duk-Young
    • Speech Sciences
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    • v.13 no.2
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    • pp.47-58
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    • 2006
  • People tend to control their speech rate to help those with listening problems such as hearing impaired people. The aim of this study was to investigate effects of speech rate on the sentence perception by 10 adults with cochlear implantation. The sample speech included 42 sentences at normal, slow, and very slow speed focusing on the overall duration, vowel or pause duration. The subjects listened to the speech and wrote down what they heard. Each correct syllable of the content words in the sentence was counted to obtain the score. Partial points were given to the incomplete syllables. Results of this study were as follows: 1. The changes of speech rate had some influence on the sentence perception score by the cochlear implanted people. 2. In slow pause condition, the controlled speech rate had a positive effect on the perception score.

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Applications of the wavelet transform in the generation and analysis of spectrum-compatible records

  • Suarez, Luis E.;Montejo, Luis A.
    • Structural Engineering and Mechanics
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    • v.27 no.2
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    • pp.173-197
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    • 2007
  • A wavelet-based procedure to generate artificial accelerograms compatible with a prescribed seismic design spectrum is described. A procedure to perform a baseline correction of the compatible accelerograms is also described. To examine how the frequency content of the modified records evolves with time, they are analyzed in the time and frequency using the wavelet transform. The changes in the strong motion duration and input energy spectrum are also investigated. An alternative way to match the design spectrum, termed the "two-band matching procedure", is proposed with the objective of preserving the non-stationary characteristics of the original record in the modified accelerogram.

Physicochemical Characteristics and Antioxidant Activities of Doragi (Platycodon grandiflorum) at Different Aging Temperatures and for Various Durations (숙성온도와 기간에 따른 도라지의 이화학적 특성과 항산화 활성)

  • Lee, Sang Hoon;Song, Eun Mi;Jang, Gwi Yeong;Li, Meishan;Kim, Min Young;Park, Hye Jin;Kang, Tae Su;Jeong, Heon Sang
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.9
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    • pp.1405-1411
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    • 2013
  • We investigated the physicochemical characteristics and antioxidant activities of Doragi (Platycodon grandiflorum) at different aging temperatures (60, 70 and $80^{\circ}C$) and for various periods of duration (5, 10, 15, 30 and 50 days). As the temperature and duration were increased, the pH of Doragi water extracts decreased from 5.22 to 4.17, whereas total acidity increased from 0.265 to 0.998 lactic acid eq.%. In addition, browning index went up from 0.092 to 1.002 and 5-hydroxymethylfurfural (HMF) content steeply rose to 50.40 mg/g from its initial zero value with an increase in temperature and duration. The radical scavenging activity of ethanol extracts was enhanced with a rise in temperature and duration as evident from the value of total polyphenol content (0.589 to 2.358 mg/g), DPPH (0.149 to 1.244 mg Trolox eq/g) and ABTS (0.354 to 1.509 mg Trolox eq/g). The correlation between physicochemical characteristics and antioxidant activities was high; the correlation between pH and total acidity showed a r value of -0.910 (P<0.01), whereas between browning index and 5-HMF content, the r value was 0.880 (P<0.01). Total polyphenol content and DPPH and ABTS radical scavenging activity were highly correlated with the r value of 0.885 (P<0.01) and 0.745 (P<0.01), respectively.

Changes in Quality of according to Fermentation Time of Fermented Soybean Produced Made with Bacillus amyloliquefaciens (Bacillus amyloliquefaciens로 제조한 콩 발효물의 발효시간에 따른 품질 변화)

  • Shin, Dong Sun;Choi, In Duck;Park, Ji Young;Kim, Nam Geol;Lee, Seuk Ki;Jeong, Kwang-Ho;Park, Chang Hwan;Choi, Hye Sun
    • The Korean Journal of Food And Nutrition
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    • v.33 no.4
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    • pp.381-389
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    • 2020
  • The purpose of this study was to evaluate the appearance, physicochemical, physical, and fermentation properties of the fermented soybean produced by manufacturing with inoculation the different types of microbial strains. The strains were inoculated by the NSI (natural strains inoculation), and the SSI (selective strain inoculation) were treatments. The appearance showed differences in color, viscous substance, and hardness depending on strains inoculation and fermentation duration. The pH, and total acidity were 6.40~7.26%, and 0.10~0.39% respectively with differences depending on the samples. The moisture content as the fermentation duration increased, the NSI (56.03~57.66%) decreased and the SSI (56.71~58.63%) increased. The physical characteristics of the hardness increased as the fermentation duration increased for the NSI and the SSI decreased. The color values for the L, a, and b values were 47.64~58.56, 7.15~9.08, and 12.41~17.30, respectively. The α-amylase and protease activities of the SSI were the highest among all treatments. The total viable cell counts of the fermented soybean products by strains were 5.02 to 9.77 log CFU/g, and SSI (fermentation, 48 hours) was the highest. The amino-type nitrogen contents of all samples were 301.62~746.97 mg% and the SSI showed the highest content. The amino acid had the highest glutamic acid content.

Studies on the General Composition, Rheometric and Microbiological Change of Pacific Saury, Coloabis saira Kwamaegi on the Storage Temperatures and Durations (저장 온도와 저장 기간에 따른 꽁치과메기의 일반 성분, 물성 및 미생물학적 변화)

  • Lee, Ho-Jin;Oh, Seung-Hee;Choi, Kyoung-Ho
    • The Korean Journal of Food And Nutrition
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    • v.21 no.2
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    • pp.165-175
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    • 2008
  • Pacific saury, Cololabis saira kwamaegi, is a traditional local food of the Eastern sea area centering around Pohang. It is well-recognized as being both tasty and nutritious. Nevertheless, bacterial contamination, excessive dryness, and compositional changes have made it edible only during the winter months. Therefore, to improve its storage, this study examined the effects of storage material, type, temperature, and duration on compositional changes in kwamaegi. The studied samples were kwamaegis that had been dried naturally for 15 days. The storage materials included an A-film, a self-developed multi-film made of polyethylene, polyamide, EVOH, and polyethylene; as well as a B-film made of polyethylene, nylon, polyethylene, nylon and polyethylene. The B films were used after pressing and laminating. The storage types included one whole fish(1G), or 2 divided fish(2G), to increase eating convenience. The 2G type was the muscle portion divided vertically after discarding the jowl, skin, and internal organs. The storage temperatures were $0^{\circ}C$, $-15^{\circ}C$, and $-30^{\circ}C$, and the storage durations were 2, 4, and 6 months. Pathogenic bacteria and rheology were measured to observe general compositional changes. The whole kwamaegi showed a total cell number of $1,565{\pm}112$ CFU/100 g flesh, while the divided Kwamaegi showed significantly greater bacterial numbers at $2,031{\pm}145$ CFU/100 g flesh. Psychrophils and halophils increased significantly while coliform were not found; the number of mesophils also increased, but not significantly. There were no significant cell number variations between the A-film and B-film. At $0^{\circ}C$, both the A-and B-films resulted in cell numbers of $115{\sim}212$ CFU/100 g flesh, revealing just $7.3{\sim}10.4%$ of the initial storage levels. Overall, there were no significant differences between the storage materials. Generally, as the storage temperature and duration increased, the moisture content of the kwamaegi decreased. Also, as storage duration and temperature increased, crude protein and crude lipid contents increased; in addition, they increased proportionally as the moisture content of the fish decreased. There were no significant differences in crude ash content with respect to the storage materials, storage temperatures, or storage durations. Finally, there were no significant differences between the kwamaegi samples naturally dried for 15 days and those stored in the B-film vacuum storage for 6 months for strength, hardness, cohesiveness, springiness, gumminess, and water activity.

Application of Predictive Food Microbiology Model in HACCP System of Milk (우유의 HACCP 시스템에서 Predictive Food Microbiology Model 이용)

  • 박경진;김창남;노우섭;홍종해;천석조
    • Journal of Food Hygiene and Safety
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    • v.16 no.2
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    • pp.103-110
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    • 2001
  • Predictive food microbiology(PFM) is an emerging area of food microbiology since the later 1980’s. It does apply mathematical models to predict the responses of microorganism to specified environmental variables. Although, at present, PFM models do not completely developed, models can provide very useful information for microbiological responses in HACCP(Hazard Analysis Critical Control Point) system and Risk Assessment. This study illustrates the possible use of PFM models(PMP: Pathogen Modeling Program win5.1) with milk in several elements in the HACCP system, such as conduction of hazard analysis and determination of CCP(Critical Control Points) and CL(Critical Limits). The factors likely to affect the growth of the pathogens in milk involved storage fixed factors were pH 6.7, Aw 0.993 and NaCl 1.3%. PMPwin5.1 calculated generation time, lag phase duration, time to level of infective dose for pathogens across a range of storage (Critical Control Points) and CL(Critical Limits). The factors likely to affect the growth of the pathogens in milk involved storage temperature, pH, Aw and NaCl content. The factors likely to affect the growth of the pathogens in milk involved storage temperature, pH, Aw and NaCl content. The variable factor was storage temperature at the range of 4~15$^{\circ}C$ and the fixed factors were pH 6.7, Aw 0.993 and NaC 1.3%. PMPwin5.1 calculated generation time, lag phase duration, time to level of infective dose for pathogens across a range of storage temperature.

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Quality of Sweet Corn Stored at Different Temperatures and Duration (저장온도와 기간에 따른 단옥수수의 품질변화)

  • 이석순;이상직;김대연
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.32 no.2
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    • pp.137-143
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    • 1987
  • To find out the optimum storage temperature and duration, changes in the content of sugars and soluble solids and flavor rate of two sweet corn hybrids (Great Bell and Golden Cross Bantam) and a super sweet corn (Crisp Super Sweet 720) were observed after storing at -20, 0, 5, 10, 15, 20, and 25$^{\circ}C$ for 1,3,5, and 7 days. At the temperatures below 10$^{\circ}C$, contents of soluble solids and total sugars and flavor rate were not changed significantly, but at the temperatures higher than 15$^{\circ}C$ they decreased as storage temperatures increased and duration extended. Storage duration conserving flavor seems to be 7 days at temperatures below 10$^{\circ}C$, 3 days at 15$^{\circ}C$, and 1-2 days at 25$^{\circ}C$. When corn was frozen, flavor rate was a little low compared with corn stored at 0$^{\circ}C$ although sugar content was higher. Both soluble solids and total sugar contents were positively correlated with flavor rate of cooked corn.

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Changes in the Riboflavin Content of Stir-Fried Sausage with Vegetable and Stir-Fried Garlic Young Stem at Various Cooking and Holding Processes in Food Service Establishments( I ) (단체급식소에서 제공되는 소시지-채소 볶음과 마늘종 볶음의 생산단계 및 보관단계에 따른 리보플라빈 함량 변화(1))

  • 김혜영
    • Korean journal of food and cookery science
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    • v.18 no.4
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    • pp.461-469
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    • 2002
  • In order to develop a better food preparation method in terms of vitamin retention in food service establishments, the retention rate of riboflavin in two kinds of Korean dish (stir-fried sausage with vegetables, stir-fried garlic young stems) were examined during various cooking stages, holding temperature and duration time. Riboflavin content of the sample was analyzed using by using a HPLC with a fluorescent detector at various holding durations and temperatures. Also the changes in the pH and water contents of the samples were measured during holding at various temperatures to find any relation among the pH, water contents and riboflavin content. In the results, there were significant differences in riboflavin contents at various cooking processes such as washing, slicing, blanching, soaking, etc. Also, the retention rates of riboflavin at various holding methods and temperature were significantly different from one another. Especially 50% reduction of riboflavin content occurred during blanching of young garlic stem and only 38% of riboflavin was left after slicing carrots for stir-frying of sausage.

Biodrying of municipal solid waste under different ventilation periods

  • Ab Jalil, N.A.;Basri, H.;Basri, N.E. Ahmad;Abushammala, Mohammed F.M.
    • Environmental Engineering Research
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    • v.21 no.2
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    • pp.145-151
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    • 2016
  • Biodrying is a pre-treatment method that applies biological and mechanical concepts to drying solid waste. In Malaysia, municipal solid waste (MSW) is unseparated and contains a high level of moisture, making the use of technology such as solid waste burning unsuitable and harmful. MSW containing organic material can be processed naturally until the moisture content of the waste is reduced. This study on MSW biodrying was carried out on a laboratory scale to measure the percent moisture content reduction and to monitor temperature patterns under different ventilation periods. This work was conducted using five biodrying reactors volumes of 50 liters each. Reactors were ventilated for 5, 10, 15, 20 and 30 min every 3 h, with a 3 bar air supply. The duration of this process was 14 days for all samples. The results showed that the optimum ventilation time was 10 min, with an 81.84% reduction in moisture content, and that it required almost half of the electricity cost required for the 20 and 30 min ventilations.