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Earlier Metabolizable Energy Intake Level Influences Heat Production during a Following 3-Day Fast in Laying Hens

  • Ning, D.;Guo, Yuming;Wang, Y.W.;Peng, Y.Z.
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.4
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    • pp.558-563
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    • 2013
  • The present study was conducted to estimate energy requirements for maintenance in laying hens by using indirect calorimetry and energy balance. A total of 576 28-wk-old Nongda-3 laying hens with dwarf gene were randomly allocated into four ME intake levels (86.57, 124.45, 166.63 and 197.20 kcal/kg body weight $(BW)^{0.75}$ per d) with four replicates each. After a 4 d adaptation period, 36 hens from one replicate were maintained in one of the two respiration chambers to measure the heat production (HP) for 3 d during the feeding period and subsequent 3 d fast. Metabolizable energy (ME) intake was partitioned between heat increment (HI), HP associated with activity, fasting HP (FHP) and retained energy (RE). The equilibrium FHP may provide an estimate of NE requirements for maintenance (NEm). Results showed that HP, HI and RE in the fed state increased with ME intake level (p<0.05). Based on the regression of HP on ME intake, the estimated ME requirements for maintenance (MEm) was 113.09 kcal/kg $BW^{0.75}$ per d when ME intake equals HP. The FHP was decreased day by day with the lowest value on the third day of starvation. Except for lowest ME intake level, the FHP increased with ME intake level on the first day of starvation (p<0.05). The FHP at the two higher ME intake levels were greater than that at the two lower ME intake levels (p<0.05) but no difference was found between the two lower ME intake levels. Linear regression of HP from the fed state to zero ME intake yielded a value of 71.02 kcal/kg $BW^{0.75}$ per d, which is higher than the extrapolated FHP at zero ME intake (60.78, 65.23 and 62.14 kcal/kg $BW^{0.75}$ per d for the first, second and third day of fasting, respectively). Fasting time, lighting schedules, calculation methods and duration of adaptation of hens to changes in ME intake level should be properly established when using indirect calorimetry technique to estimate dietary NE content, MEm and NEm for laying hens.

Ultrasonographic Features of Medullary Thyroid Carcinoma: Do they Correlate with Pre- and Post-Operative Calcitonin Levels?

  • Cho, Kyung Eun;Gweon, Hye Mi;Park, Ah Young;Yoo, Mi Ri;Kim, Jeong-Ah;Youk, Ji Hyun;Park, Young Mi;Son, Eun Ju
    • Asian Pacific Journal of Cancer Prevention
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    • v.17 no.7
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    • pp.3357-3362
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    • 2016
  • Purpose: To correlate ultrasonographic (US) features of medullary thyroid carcinoma (MTC) with preoperative and post-operative calcitonin levels. Materials and Methods: A total of 130 thyroid nodules diagnosed as MTC were evaluated. Two radiologists retrospectively evaluated preoperative US features according to size, shape, margin, echogenicity, type of calcification, and lymph node status. Postoperative clinical and imaging follow-up (mean duration $31.9 {\pm} 22.5$ months) was performed for detection of tumor recurrence. US features, presence of LN metastasis, and tumor recurrence were compared between MTC nodules with and without elevated preoperative calcitonin (>100 pg/mL). Those with normalized and non-normalized postoperative calcitonin levels groups were also compared. Results: Common US features of MTCs were solid internal content (90.8%), irregular shape (44.6%), circumscribed margin (46.2%), and hypoechogenicity (56.2%). Comparing MTC nodules with and without elevated preoperative calcitonin levels, the size and shape of MTC nodule and lymph node metastasis showed statistical significance (p<0.05). Postoperative calcitonin normalization correlated with US features of tumor size (p=0.002), margin (p=0.034), shape ($p{\leq}0.001$), and presence of calcification (p=0.046). Tumor recurrence and LN metastasis were more prevalent in patients without normalization of postoperative calcitonin than in those with normalization (p=0.001). Conclusions: Serum calcitonin measurement is helpful for early diagnosis and predicting prognosis. Postoperative calcitonin measurement is also important for postoperative US follow up, especially in cases with larger nodule size, presence of calcification, irregular shape, and irregular margin.

Direct Effect of a Hot Environment on Ruminal Motility in Sheep

  • Sunagawa, Katsunori;Arikawa, Yuji;Higashi, Mika;Matsuda, Hiroshi;Takahashi, Hiroshi;Kuriwaki, Zyunichi;Kojiya, Zuikou;Uechi, Syuntoku;Hongo, Fujiya
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.6
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    • pp.859-865
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    • 2002
  • The aim of this research was to clarify the direct effects of a hot environment on ruminal motility in sheep fed twice a day. In the first experiment, in order to equalize variable factors excluding the ambient temperature between the thermoneutral environment ($23^{\circ}C$, relative humidity 80%) and the hot environment ($32^{\circ}C$, relative humidity 80%), sheep were fed equal amounts of the same quality feed twice a day. The sheep were allowed free access to water for the duration of the two one-hour feeding periods (10:00 am-11:00 am, 5:00 pm-6:00 pm). On the fourth day after exposure to the hot environment, the frequency and strength of ruminal contractions were continuously recorded between 9:30 am and 11:00 pm. Prior to the exposure to a hot environment the frequency and strength of ruminal contractions were recorded in a thermoneutral environment during the period 9:30 am-11:00 pm. In the second experiment, in order to maintain the stomach content of the sheep at equal levels in both environments, the sheep were fed equal amounts of the same quality feed twice a day. Following the completion of the two one-hour feeding periods, a fixed amount of warm water was infused into the rumen. Rumen motility was then recorded during the same period as for the first experiment (9:30 am-11:00 pm). In the first experiment, when the frequency of ruminal contractions prior to (24, 24 frequency/15 min), during (48, 47 frequency/min) and after (22, 19 frequency/min) both the morning and afternoon feeding in a hot environment was compared with the values from the thermoneutral environment (20, 22; 50, 50; 21, 20 frequency/min), there was found to be no difference. However, the strength of ruminal contractions after morning and afternoon feeding (3.7, 3.1 mm Hg) in the hot environment decreased significantly in comparison with the thermoneutral environment (4.3, 3.8 mm Hg). In the second experiment, the frequency of ruminal contractions in the hot environment was not significantly different from that in the thermoneutral environment. The strength of ruminal contractions after ruminal infusion of warm water in the hot environment (morning: 4.6, afternoon: 4.5 mm Hg) was significantly lower than that in the thermoneutral environment (morning: 5.6, afternoon: 5.0 mm Hg). The results suggest that a hot environment acts directly on the strength of ruminal contractions in sheep fed twice a day rather than on the frequency.

Detection of Serum IgA and IgE Antibodies in Experimental Animals Infected with Echinostoma hortense (호르텐스극구흡충 감염 흰쥐 및 마우스의 IgA/lgE 항체가 반응 추이)

  • Yong-Suk Ryang;Insik Kim;Kyu-Jae Lee;Ji-Sook Lee
    • Biomedical Science Letters
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    • v.6 no.2
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    • pp.93-100
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    • 2000
  • The change in mean absorbance values for IgA/IgE in rats and mice infected with Echinostoma hortense metacercariae was studied from the 2nd week to the 8th week after infection. Serum and intestinal luminal content (ILC) levels of IGA/IGE were measured by enzyme-linked immunosorbent assay(ELISA). The mean absorbance values obtained from IgA in the rats' ILC increased from the 2nd week to the 8th week after infection. The peak value (0.47$\pm$0.01) appeared in the 8th week. The mean absorbance values of IgE in the rats' ILC didn't increase significantly (p>0.05). The worm recovery rate decreased at a slower, pace after, infection. The duration in which the peak value of IgA in rats' ILC appeared was similar to that in which the worm recovery rate declined significantly. Serum levels of IgA/IgE in mice increased gradually from the 2nd week after infection. The peak value (0.45$\pm$0.01) of IgA appeared in the 8th week, and that (0.23$\pm$0.02) of IgE appeared in the 7th week after infection. The ILC level of IgA in mice continued to increase after infection, and reached its peak in the 8th week. The change in IgA/lgE in the serum and IgA in the ILC of mice was inversely related to worm recovery rate. As a result of this experiment, it is supposed that IgA/IgE may play an important role in the expulsion of Echinostoma hortense.

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Cadmium, Lead, and Zinc Accumulation in Rice Grown at Paddy Soils near Old Zinc-Mining Sites (아연광산(亞鉛鑛山) 인근답(隣近沓)의 토양중(土壤中) 중금속함량(重金屬含量)과 현미중(玄米中) 함량(含量)과의 관계(關係))

  • Yoo, Sun-Ho;Park, Moo-Eon;Ro, Hee-Myoung
    • Korean Journal of Environmental Agriculture
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    • v.2 no.1
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    • pp.18-23
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    • 1983
  • Effect of Cd, Pb, and Zn content in soil on their accumulation in rice was studied by analyzing brown rice(93) and soil samples(180) collected from paddy soils near old zinc-mining sites in 1979 and 1980. Ratio of Cd, Pb, and Zn in brown rice to soil decreased with the increase of their contents in soil and found to be linear function of the inversed values of their contents in soil. Contents of Cd, Pb, and Zn in brown rice harvested in 1980 were lower than those in 1979. The significant difference in contents of Pb and Zn between two years might be attributed to weather. Air temperature and duration of sunshine in 1980 were significantly lower than those in 1979.

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Effects of Depth and Duration of Flooding on Growth and Yield at Different Growth Stage in Pepper(Capsicum annuum L.);I. Response to Flooding at Seedling Stage (고추(Capsicum annuum L.)의 생육단계별(生育段階別) 침수처리(浸水處理)에 따른 생육반응(生育反應);I. 유묘기(幼苗期) 반응(反應))

  • Guh, Ja-Ock;Kuk, Yong-In
    • Korean Journal of Environmental Agriculture
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    • v.15 no.3
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    • pp.325-334
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    • 1996
  • Pepper plants were flooded at 0, 5, 10 and 15cm at seedling stage under the condition of greenhouse. Treatment of flooding times are 6, 12, 24, 48 and 120 hours. The results obtained are summarized as follows. Plant height, number of leaves, shoot and root fresh weight were not recovered the flooding damages regardless of flooding time and depth. Pepper plant died in flooding depth of 5cm or more for over 48 hours. Plants in fallen leaves were found at more than 5cm depth and 6 hours of floodings. Photosynthesis and respiration rate decreased in the 5cm flooding depth or more for 24 hours. Chlorophyll content and root activity decreased for 12 hours or more at all the flooding depth. Also, diffusion resistance of stomata cell increased as increased flooding time and depth. Diseases occurred remarkably in proportion to the depth and hours of flooding treatment. It was not possible to control the desease by fungicide, also then was no effects of foliar spray of urea. Weight of fruit per plant not decrease by the 12 hours of 0cm and the 6 hours of 5cm flooding but decreased at deeper and longer flooding. Average weight of a fruit increased. The yield could not expected in the depth of 5cm or more for over 48 hours, There was significant positive correlation between all the investigated characteristics of growth and yield. There was, however, negative correlation between number of leaf and diffusion resistance of stomata.

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Absorbed Dose Measurement by the MIRD System in the $^{131}I$ Treated Thyroid Cancer Patients (갑상선 암 환자에서 $^{131}I$ 치료시 MIRD Schema에 의한 흡수선량의 평가)

  • Lim, Sang-Moo;Woo, Kwang-Sun;Chung, Wee-Sup;Hong, Sang-Woon;Kim, Jang-Hee;Kim, Ki-Sup
    • The Korean Journal of Nuclear Medicine
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    • v.29 no.1
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    • pp.54-60
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    • 1995
  • Medical Internal Radiation Dose(MIRD) schema was developed for calculating the absorbed dose from the administered radiopharmaceuticals. With the biological distribution data and the physical properties of the radionuclide we can estimate the absorbed dose by the MIRD schema. For the thyroid cancer patients received $^{131}I$ therapy, the absorbed dose to the bone marrow is the limiting factor to the administered dose, and the duration of admission is deter-mined by the retained activity in the whole body. To monitor the whole body radioactivity, we used Eberline Smart 200 system using ionization chamber as a detector. With the time activity curve of the whole body, total body residence time was obtained. From the ICRP publication 53, the residence times of the source organs, such as kidney, urinary bladder content and stomach, were used to calculate the absorbed doses of the target organs, such as stomach, red marrow, bladder wall and remaineder total body. In 8 thyroid cancer patients with 175 mci of $^{131}I$ administered orally, the mean absorbed dose in the bladder wall was 375.1, in the stomach 285.1, red marrow 25.4 and total body 22.4 rad respectively. For the monitoring of the large administered activity, this method seemed to be quite useful.

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Determination of Optimum Stepped Vacuum Pressure and Settlement for IVPM-applied Ground (개별진공압공법이 적용된 지반의 최적 단계진공압 산정 및 침하예측)

  • Yoon, Myung-Seok;Ahn, Dong-Wook;Park, Jea-Man;Kim, Soo-Sam
    • Land and Housing Review
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    • v.2 no.2
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    • pp.163-170
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    • 2011
  • Individual Vacuum Pressure Method (IVPM) is a soft ground improvement technique, in which a vacuum pressure can be directly applied to the vertical drain board to promote consolidation and to strengthen the soft ground. This method does not require surcharge loads, different to embankment or pre-loading method. In this study, the ground improvement efficiency of Individual Vacuum Pressure Method was estimated when suction pressure increases step by step(-20, -40, -60, -80kPa) with different periods. During Individual Vacuum Pressure Method process, surface settlement and pore pressure were monitored, and cone resistance as well as water content were also measured after the completion of Individual Vacuum Pressure Method treatment. From the results, optimum duration of each step of vacuum pressure was determined, and the settlement was calculated using FEM numerical analysis.

Characteristics of Seedling and Rhizome Propagation in Epimedium koreanum NAKAI (삼지구엽초 실생 및 지하경번식 특성)

  • Noh, Joon-Hyun;Kim, Young-Jin;Choi, Kang-Jun;Kim, Se-Won;Kim, Sung-Kyeong;Kim, Jong-Hwa
    • Korean Journal of Medicinal Crop Science
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    • v.11 no.2
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    • pp.155-160
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    • 2003
  • This study was carried out to investigate propagation characteristics of seedling and rhizome in Epimedium koreanum NAKAI. Seed germination essentially needed after-ripening duration after harvesting for 250days and more by stratification. At $20^{\circ}C$ seed emergence days and root length were 8 and 0.46cm respectively. The above-ground part of E. koreanum grew until 60days after root cutting and the underground part of it grew after that time. The optimum amount of organic fertilizer was fermented cow-manure with husk 2,000kg/10a that showed $57.5cm^2$ in leaf area per plant, 6.2cm in new rhizome length, and 0.43% in icariin content, an index component of E. koreanum, while inorganic N-fertilizer made E. koreanum worse as it increased. In nutriculture extruded rice hull media was better for growth than perlite media at 1/2 NHRI solution.

Effects of Farming Practice and NO3-N Contents of Groundwater with Different locations under Intensive Greenhouse Area (시설재배지의 재배방법 및 입지적 조건이 지하수 NO3-N 함량에 미치는 영향)

  • Ko, Jee-Yeon;Lee, Jae-Sang;Kim, Min-Tae;Kim, Choon-Song;Kang, Ui-Gum;Kang, Hang-Won
    • Korean Journal of Environmental Agriculture
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    • v.24 no.3
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    • pp.261-269
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    • 2005
  • To investigate effects of fanning practice and $NO_3-N$ contents of groundwater in intensive horticultural greenhouse area of Yeongnam province, the groundwater samples from 1,370 sites were collected and analyzed. In addition, cultivation year, crops, desalinization methods, amounts of manure application, tube well depth and soil texture with clay contents were observed. Of the investigated sites, 19.7% of shallow groundwater and 1.3% of confined groundwater were exceeded over $10mg/{\ell}$ as the $NO_3-N$ drinking limit value, respectively. $NO_3-N$ concentration in groundwater was highly significantly correlative with clay content $(r=-0.241^{**})$, well depth $(r=-0.228^{**})$ and cultivation duration $(r=0.104^*)$, respectively. In case of desalting crop method being executed for desalinization of greenhouse soil, the $NO_3-N$ value of groundwater was lower than any other desalinization method. The fact that $NO_3-N$ contents of confined groundwater was affected by shallow groundwater was clarified by that $NO_3-N$ contents in shallow were significantly correlative with the confined groundwater $(r=0.532^{**})$.