• Journal of Microbiology and Biotechnology
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• 제27권2호
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• pp.335-341
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• 2017

The complexity of the bacterial recombination system is a barrier for the construction of bacterial mutants for the further functional investigation of specific genes. Several protocols have been developed to inactivate genes from the genus Pseudomonas. Those protocols are complicated and time-consuming and mostly do not enable easy construction of multiple knock-ins/outs. The current study describes a single and double crossover-recombination system using an optimized vector-free allele-exchange protocol for gene disruption and gene replacement in a single species of the family Pseudomonadaceae. The protocol is based on self-ligation (circularization) for the DNA cassette which has been obtained by overlapping polymerase chain reaction (Fusion-PCR), and carries an antibiotic resistance cassette flanked by homologous internal regions of the target locus. To establish the reproducibility of the approach, three different chromosomal genes (ncRNA31, rpoN, rpoS) were knocked-out from the root-associative bacterium Pseudomonas stutzeri A1501. The results showed that the P. stutzeri A1501 mutants, which are free of any plasmid backbone, could be obtained via a single or double crossover recombination. In order to optimize this protocol, three key factors that were found to have great effect on the efficiency of the homologous recombination were further investigated. Moreover, the modified protocol does not require further cloning steps, and it enables the construction of multiple gene knock-in/out mutants sequentially. This work provides a simple and rapid mutagenesis strategy for genome editing in P. stutzeri, which may also be applicable for other gram-negative bacteria.

• 한국건축시공학회지
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• 제23권2호
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• pp.113-121
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• 2023

본 연구에서는 부산물(석분 슬러지, 고로슬래그, 제강슬래그, 폐콘크리트 슬러지, 바텀애시) 5종과 클링커 내 중금속 분석을 위한 레이져 유도 플라즈마 분광법(LIBS) 적용성과 재현성 검토를 실험적으로 수행하였다. 클링커를 포함한 6종의 부산물 시료에 대한 ICP-MS, XRF, LIBS 분석 결과, 정량적 분석에서 ICP와 XRF의 차이가 확인되었으나, LIBS 분석은 표준분석과 일치하지 않고 시료의 정성적인 분석만 가능하였다. LIBS를 통한 정량적 분석을 위하여 Cd, Pb, Hg에 대하여 LIBS 분석 파장(Cd - 214.44nm, Pb - 405.78nm, Hg - 253.65nm)을 설정하였고, 농도별(1~1000ppm) 용액에 함침하여 건조시킨 멤브레인 표면에 레이져를 조사한 결과, 스펙트럼 강도와 농도 사이의 상관관계를 확인할 수 있었다.

• Computers and Concrete
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• 제15권6호
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• pp.903-919
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• 2015

The flow of freshly mixed cement-based material shows thixotropy, which implies some difficulties on robust measurement of its rheological properties: The flow curve of thixotropic materials depends on the used protocol. For examples, higher viscosity is obtained when the rate of shear strain is more quickly increased. Even though precise measurement and modelling of the concrete rheology needs to consider the thixotropic effect, engineers in the concrete field prefer considering as a non-thixotropic Herschel-Bulkley fluid, even more simply Bingham fluid. That is due to robustness of the measurement and application in casting process. In the aspect of simplification, this papers attempts to mimic the thixoropic flow by the non-thixotropic Herschel-Bulkley model. Disregarding the thixotropy of cement based materials allows us to adopt the rheological concept in the field. An optimized protocol to measure the Bingham parameters was finally found based on the accuracy and reproducibility test of cement paste samples, which minimizes the error of simulation stemming from the assumption of non-thixotropy.

• The Plant Pathology Journal
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• 제35권2호
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• pp.164-171
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• 2019

An assay for detecting Apple scar skin viroid (ASSVd) was developed based on nucleic acid sequence based amplification (NASBA) in combination with realtime detection during the amplification process using molecular beacon. The ASSVd specific primers for amplification of the viroid RNA and molecular beacon for detecting the viroid were designed based on highly conserved regions of several ASSVd sequences including Korean isolate. The assay had a detection range of $1{\times}10^4$ to $1{\times}10^{12}$ ASSVd RNA $copies/{\mu}l$ with reproducibility and precision. Following the construction of standard curves based on time to positive (TTP) value for the serial dilutions ranging from $1{\times}10^7$ to $1{\times}10^{12}$ copies of the recombinant plasmid, a standard regression line was constructed by plotting the TTP values versus the logarithm of the starting ASSVd RNA copy number of 10-fold dilutions each. Compared to the established RT-PCR methods, our method was more sensitive for detecting ASSVd. The real-time quantitative NASBA method will be fast, sensitive, and reliable for routine diagnosis and selection of viroid-free stock materials. Furthermore, real-time quantitative NASBA may be especially useful for detecting low levels in apple trees with early viroid-infection stage and for monitoring the influence on tree growth.

• 한국의류산업학회지
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• 제24권3호
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• pp.315-324
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• 2022

This study aims to verify the sewing technology and the expression of the virtual garment program by comparing the appearance similarity between the real and the virtual power shoulder jacket. To this end, the H and the X silhouettes of the power shoulder jacket were selected as the reference designs. After making the reference designs into real jackets by applying the human body dimensions of women in their twenties, virtual avatars and jackets were produced using CLO 3D programs. The results showed that the H-type with only expanded shoulders and less design variation had a high similarity between the reference photo and real garment, the real and virtual garment, and the reference photo and virtual garment. The power shoulder jacket of the reference picture was well reproduced in the real and virtual garments. Conversely, the X-type jacket, which is a tight fit and has many design variations, showed somewhat low similarity in the circumference items and the basic construction line. Especially in tight-fitted power shoulder jackets, the fact that shoulder angles and the essential items for pattern making cannot be reflected in reproducing virtual models was found to be a limitation in increasing the similarity of virtual clothing. Furthermore, the sewing system gap between the real and the virtual clothing exposed a difference in the appearance of virtual clothing.

• 한국건설순환자원학회논문집
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• 제10권4호
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• pp.435-442
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• 2022

본 연구에서는 시멘트, 고로슬래그 미분말 및 플라이 애시의 효율적인 밀도 관리를 위해 KS L 5110과 가스 피크노미터 및 전자 밀도계에 의한 밀도 값을 비교하였다. 시험방법에 따른 상관관계 및 사용성을 검토하였으며, 이를 바탕으로 대체 시험방법에 대한 활용 가능성을 분석하였다. 시험방법에 따른 밀도 시험결과 시멘트, 고로 슬래그 미분말 및 플라이 애시 모두 가스 피크노미터, KS L 5110 및 전자 밀도계 순으로 밀도 값이 낮아지는 경향을 나타냈으며, 이는 시험방법에 따라 시료의 부피를 평가하는 범위의 차이에 따른 것으로 분석된다. 시험방법에 따른 상관관계는 결정계수 R² 값이 0.71~0.93 범위로 비교적 양호한 상관성을 나타냈으며, 상관식을 통해 오차에 대한 보정을 적용하면 대체 시험방법의 활용이 가능할 것으로 분석된다. 시험 절차, 측정시간 및 변동계수를 고려한 사용성 검토 결과 가스 피크노미터는 시험 절차가 가장 간단하며, 신뢰성도 양호하였다. 또한, 숙련도가 크게 필요하지 않기 때문에 시험자 간의 재현성도 상대적으로 높을 것으로 예상된다.

• 한국지반환경공학회 논문집
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• 제15권8호
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• pp.45-50
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• 2014

하수관 내에서의 하수와 미생물의 반응에 대한 실험은 이미 매설된 하수관에 적용할 경우 실험조건의 변화가 제한적이며 실험시간에도 한계가 있다. 본 연구에서는 이러한 하수관 내에서의 하수와 미생물의 반응에 대하여 DO를 제한 조건으로 설정하였을 경우, 실험실 내에서 실험반응조를 통해 재현하는 방법에 대하여 연구하였다. 하수관 내에서의 DO 농도는 기상 중의 산소가 하수 내로 이동하는 재포기현상에 의해 조절된다. 이러한 현상을 이용하여 임의로 실험반응조에서 하수관 내의 유하조건을 조절하고 재포기현상을 재현하여 유하조건과 재포기계수와의 상관관계를 얻을 수 있으며, 교반강도(속도경사)에 따른 재포기계수와 하수관거에서의 유하조건에 따른 재포기계수의 상관관계를 통해 동일한 재포기계수 값을 갖는 교반강도와 유하조건을 설정할 수 있다. 이러한 결과를 통해 재포기계수를 제한인자로 설정하여 실험을 실시할 경우 하수관거에서의 상황을 실험반응조를 통해 재현할 수가 있었다.

• 약학회지
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• 제52권6호
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• pp.480-487
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• 2008

We described an estimation of measurement uncertainty in quantitative analysis of 11-nor-9-carboxy-${\Delta}^9$-tetrahydrocannabinol (THCCOOH), the major metabolite of ${\Delta}^9$-tetrahydrocannabinol, in urine sample by solid-phase extraction (SPE) and GC/MS detection. The analytical results were compared and the different contributions to the uncertainty were evaluated. Inter-day and inter-person validation were performed using statistical analysis of several indicative factors. Measurement uncertainty associated with target analyte in real forensic samples was estimated using quality control (QC) data. Traceability of measurement was established through traceable standards, calibrated volumetric glassware and volume measuring device. The major factors of contribution to combined standard uncertainty, were calibration linearity, inter-day repeatability and inter-person reproducibility, while those associated with preparation of analytical standards and sampling volume were not so important considering the degree of contribution. Relative combined standard uncertainties associated with the described method was 12.05% for THCCOOH.

• 한국건축시공학회지
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• 제23권6호
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• pp.715-726
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• 2023

본 연구에서는 수소 충전소와 같은 폭발 가능성을 가진 구조물들의 안전 설계 방안으로 방호패널이 희생 부재로 설치되어 구조물의 직접적인 피해를 최소화하고 회복 탄력성을 가질 수 있어야 한다고 제시하였다. 이를 위해 비상체의 고속 충돌 상황에서 방호 패널이 구조물과 밀착 또는 이격 설치되었을 때 구조물이 받는 영향을 정량적으로 평가하는데 실험의 초점을 맞추고 있다. 실험 설계는 우수한 재현성을 위해 기존에 주로 사용한 콘크리트 구조부재 대신 강판을 사용하며, 철판 배면의 변형 차이를 통해 구조부재의 충격을 비교·분석하였다. 또한, 방호 부재의 이격재로 사용한 탄성체의 물성 변화와 방호부재 및 탄성체에 따른 충격파 전달 시간의 차이가 구조부재에 미치는 영향을 조사하였다.

• Journal of Korean Neurosurgical Society
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• 제60권4호
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• pp.441-447
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• 2017

Objective : Computed tomography (CT)-based method of three dimensional (3D) analysis ($MIMICS^{(R)}$, Materialise, Leuven, Belgium) is reported as very useful software for evaluation of OPLL, but its reliability and reproducibility are obscure. This study was conducted to evaluate the accuracy of $MIMICS^{(R)}$ system, and inter- and intra-observer reliability in the measurement of OPLL. Methods : Three neurosurgeons independently analyzed the randomly selected 10 OPLL cases with medical image processing software ($MIMICS^{(R)}$) which create 3D model with Digital Imaging and Communication in Medicine (DICOM) data from CT images after brief explanation was given to examiners before the image construction steps. To assess the reliability of inter- and intra-examiner intraclass correlation coefficient (ICC), 3 examiners measured 4 parameters (volume, length, width, and length) in 10 cases 2 times with 1-week interval. Results : The inter-examiner ICCs among 3 examiners were 0.996 (95% confidence interval [CI], 0.987-0.999) for volume measurement, 0.973 (95% CI, 0.907-0.978) for thickness, 0.969 (95% CI, 0.895-0.993) for width, and 0.995 (95% CI, 0.983-0.999) for length. The intra-examiner ICCs were 0.994 (range, 0.991-0.996) for volume, 0.996 (range, 0.944-0.998) for length, 0.930 (range, 0.873-0.947) for width, and 0.987 (range, 0.985-0.995) for length. Conclusion : The medical image processing software ($MIMICS^{(R)}$) provided detailed quantification OPLL volume with minimal error of inter- and intra-observer reliability in the measurement of OPLL.