• The Journal of Engineering Geology
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• v.26 no.1
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• pp.51-61
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• 2016

New building methods are needed to aid increased inner-city redevelopment and industrial construction. A particular area of improvement is the efficient use of cut slopes, with the minimization of associated problems. A retaining wall of precast panels can resist the horizontal earth pressure by increasing the shear strength of the ground and reinforcing it through contact with the panels. Precast panels allow quick construction and avoid the problem of concrete deterioration. Other problems to be solved include the digging of borrow pits, the disposal of material cut from the slope, and degradation of the landscape caused by the exposed concrete retaining wall.This study suggest the methods of improvement of an existing precast panel wall system by changing the appearance of the panels to that of natural rock and improving the process of adhering the panel to a vertical slope. The panels were tested in the laboratory and in the field. The laboratory test verified their specific strength and behavior, and the field test assessed the panels' ground adherence at a vertical cutting. Reinforcement of the cutting slope was also measured and compared with the results of 3D numerical analysis. The results of laboratory test, identified that the shear bar increase the punching resistance of panel. And as a results of test construction, identified the construct ability and field applicability of the panel wall system adhered to in-situ ground. In addition to that, extended measurement and numerical analysis, identified the long-term stability of panel wall system adhered to in-situ ground.

• Journal of Korean Society of societal Security
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• v.3 no.2
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• pp.41-46
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• 2010

This study focuses on two points. First, I have examined the difference on combustion between flame-retardant and non flame-retardant products including vertical blinds and roll screens that are widely used as a substitute for fabric curtains. The second point is to see how long flame-retardant goods can be durable, that is, flame retardant durability after washing in liquid. the experiment on the flame-retardant ability before and after washing of vertical blinds and roll screens that have been used for a long time in fire protection construct. Comparing and analyzing domestic and foreign laws on flame-retardant after the experiment on durability of these products. I aim to draw necessity for increased application of internal laws and regulations on flame-retardant and show how to improve the point at issue. According to the result, clear differences in performance and safety were observed between flame-retardant and non flame-retardant products. flame-retardant materials can prevent things from fire spread without igniting but melting when they've met flame and burnt, In contrast, non flame-retardant material for experiment which size is 120cm long takes less than 3 minutes only to be completely destroyed by fire. However, it was expected for flame-retardant durability of flame-retardant blinds which are not washable to decrease flame-retardant durability after being washed with water, there was no sharp difference. so it is demonstrated that flame-retardant blinds can keep flame-retardant durability. accordingly through the strict course of product we are concerned about keeping durability.

• Journal of Animal Science and Technology
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• v.62 no.4
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• pp.429-437
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• 2020

This study was conducted to construct basic data for the selection of elite cows by analyzing the estimated breeding value (EBV) and accuracy using the pedigree of Hanwoo cows in Gyeongnam. The phenotype trait used in the analysis are the carcass weight (CWT), eye muscle area (EMA), backfat thickness (BFT) and marbling score (MS). The pedigree of the test group and reference group was collected to build a pedigree structure and a numeric relationship matrix (NRM). The EBV, genetic parameters and accuracy were estimated by applying NRM to the best linear unbiased prediction (BLUP) multiple-trait animal model of the BLUPF90 program. Looking at the pedigree structure of the test group, there were a total of 2,371 cows born between 2003 to 2009, of these 603 cows had basic registration (25%), 562 cows had pedigree registration (24%) and 1,206 cows had advanced registration (51%). The proportion of pedigree registered cows was relatively low but it gradually increased and reached a point of 20,847 cows (68%) between 2010 to 2017. Looking at the change in the EBV, the CWT improved from 4.992 kg to 9.885 kg, the EMA from 0.970 ㎠ to 2.466 ㎠, the BFT from -0.186 mm to -0.357 mm, and the MS from 0.328 to 0.559 points. As a result of genetic parameter estimation, the heritability of CWT, EMA, BFT, and MS were 0.587, 0.416, 0.476, and 0.571, respectively, and the accuracy of those were estimated to be 0.559, 0.551, 0.554, and 0.558, respectively. Selection of superior genetic breed and efficient improvement could be possible if cow ability verification is implemented by using the accurate pedigree of each individual in the farms.

• The Journal of Art Theory & Practice
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• no.1
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• pp.71-92
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• 2003

This study examined the condition of an educational discourse, relating the concept of creativity, culture, culture-centered, and critical thinking, to explore Culture-based Art Education(CBAE). In particular, art education practice was examined using interpretations of creativity and critical theory positions from the field of education. Discourse analysis was used as the research method to contextually situate and analyze the ways in which art education theory and practice of creativity and of critical studies encoded meanings. The study helped build an understanding that creativity was formed as a modernist discourse in the humanistic stance. In education, creativity became the fundamental concern for progressive educators who pursued innate ability of individuals. The way to enhance creative potential of students was to induce their motive, as was the same case in art education, while in artist training, free expression was its main method. In this way, as creativity was intimately connected with the concept of expression, in art education art making is the only course for enhancing creativity. However, because creative process cannot intelligently be regarded as logically distinct from the creative product, and creativity can only be said by product, it seems valid to think that creativity is the quality not to be achieved by teaching. Furthermore, its emphasis on art making resulted in unbalance of art making and art appreciation in art education. It was the late sixties when several alternatives for creative education were made their appearance. Critical studies in art adopted critical theory as its theoretical background has developed as an alternative of creative art education, when research and theory for creativity could not be adequate to deal with the problem of practice. Critical theory is a broad and diverse field of theory and practice drawing on aspects of the modernist perspective of the later Frankfurt School, feminism, Freirean pedagogy, postcolonial discourse as well as postmodernism to construct a practical approach to education. It is very this eclectic nature to provide the mosaic that need to experience cultures from different perspectives in a pluralistic society. Because one's personality is formed by multiple aspects of culture which is very complex and is made up of what we do and value, creativity cannot make part of educational discourse with the philosophy of culture centered. On the other hand, critical studies, as a school art program of critical theory, can perform the role of CBAE, because it would have to deal with the investigation of social and cultural issues form multiple personal, local, national, and global perspectives.

• Microbiology and Biotechnology Letters
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• v.36 no.4
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• pp.260-267
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• 2008

The choice of expression vector is very important for industrial production of proteins. Therefore, the systematic mining of promoters over a wider range of genetic resource and/or host is required. We previously reported a novel bidirectional reporting system (pBGR) for the isolation of promoters from metagenome and screened useful promoters that functioned constitutively in E. coli under general culture conditions. Among them, three promoter sequences including each upstream region were amplified by PCR and used to construct new expression vectors. To facilitate subcloning, a multi-cloning site was incorporated into the downstream region of the revere primer sequence. At these sites, GFP, esterase and $\beta$-glucosidase were subcloned and analyzed the constitutive expression ability of new promoter in terms of protein solubility and expression level. As a result, these vectors expressed the proteins constitutively to a level of $2{\sim}3%$ of the total cell protein in soluble fraction (>80 %). This study suggested that excavation of metagenomic promoters for construction of expression vector in a certain strain could provide a way for the development of the expression systems.

• Journal of Microbiology and Biotechnology
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• v.28 no.4
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• pp.606-612
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• 2018

The enzyme xylose isomerase (E.C. 5.3.1.5, XI) is responsible for the conversion of an aldose to ketose, especially xylose to xylulose. Owing to the ability of XI to isomerize glucose to fructose, this enzyme is used in the food industry to prepare high-fructose corn syrup. Therefore, we studied the characteristics of XI from Anoxybacillus kamchatkensis G10, a thermophilic bacterium. First, the gene coding for XI (xylA) was inserted into the pET-21a(+) expression vector and the construct was transformed into the Escherichia coli competent cell BL21 (DE3). The expression of recombinant XI was induced in the absence of isopropyl-thio-${\beta}$-galactopyranoside and purified using Ni-NTA affinity chromatography. The optimum temperature of recombinant XI was $80^{\circ}C$ and measurement of the heat stability indicated that 55% of residual activity was maintained after 2 h incubation at $60^{\circ}C$. The optimum pH was found to be 7.5 in sodium phosphate buffer. Magnesium, manganese, and cobalt ions were found to increase the enzyme activity; manganese was the most effective. Additionally, recombinant XI was resistant to the presence of $Ca^{2+}$ and $Zn^{2+}$ ions. The kinetic properties, $K_m$ and $V_{max}$, were calculated as 81.44 mM and $2.237{\mu}mol/min/mg$, respectively. Through redundancy analysis, XI of A. kamchatkensis G10 was classified into a family containing type II XIs produced by the genera Geobacillus, Bacillus, and Thermotoga. These results suggested that the thermostable nature of XI of A. kamchatkensis G10 may be advantageous in industrial applications and food processing.

• Journal of Korea Technology Innovation Society
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• v.10 no.1
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• pp.121-142
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• 2007

The purpose of this study is to construct the evaluation model for the major science research facilities and equipments to enhance the competitiveness of the science and technology and also to test the reliability and validity of the model. To achieve the purposes, this study theoretically reviews the concept of the major science research facilities and equipments and their characteristics. Through a review of literature, this study draws 11 criteria for evaluating the priorities of the major science research facilities and equipments. These criteria are categorized as two dimensions - 'science & technology' and 'national policy'. The dimension of science & technology includes scientific importance, technological readiness, utilization rate, common utilization rate, and ability of management and operation. The national policy dimension contains degree of correspondence with national science development, imperativeness of national policy, science and technical effectiveness, economic and industrial effectiveness, responsiveness of research demand, and equity among the related institutions. The competitiveness of the science and technology consists of these two dimensions. The evaluation model is established on the framework of criteria. The 18 major science research facilities and equipments are selected through a series of Delphi. The survey of experts (BT, ET, IT, NT and ST) is also implemented to evaluate the 18 major science research facilities and equipments by 11 criteria. The overall results indicate that the reliability and validity of the model are good. The reliability tests show that the five indicators of science & technology and the six indicators of national policy have high internal consistencies. The confirmatory factor analyses reveal that the two constructs - 'science & technology' and 'national policy' - have high convergent and discriminant validity. The correlational analyses also show that the criteria-related validity between them is high. Furthermore, the results of higher order factor analysis indicate that the fit indices of the model are high and suggest a good fit to the data. Based on these findings, the policy implications of the model are discussed.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.5
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• pp.294-301
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• 2017

Recently, online social networks have become one of the most representative Internet services and are being used increasingly, which allows people to share information and interact with other people. On the other hand, as the use of OSN is increased, there has been an increased incidence where illegally accessed personal information is used wrongfully. Accordingly, privacy has become a key concern by the users. Regarding the construct of information privacy concerns (IPC), this study explored the antecedents of IPC at the individual personal level - risk appraisal and perceived behavioral control - based on Protection Motivation Theory. Hence, the current paper investigated the moderating effect of gender in the relationship between individual characteristics and IPC. The hypothesized research model was examined with 233 questionnaires of OSN users. The results showed that the positive effects of risk appraisal and the negative effects of perceived behavioral control on IPC are supported. In addition, the moderate effects of gender between risk appraisal and IPC is only supported.

• Journal of the Korea Concrete Institute
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• v.27 no.3
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• pp.291-298
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• 2015

In this paper, a bundled diagonal reinforcement using high performance steel fiber was proposed to enhance the construct ability and seismic performance. Experiments of coupling beam was composed of four specimens and the hysteretic behavior evaluated for reverse cyclic loading to specimens using high performance steel fiber. The main variables of the experiment is a amount of stirrup and bundled reinforcement, depending on whether the mix of steel fiber. Specimen which criteria was applied 100% of stirrup and bundled diagonal reinforcement of ACI318 criteria. With this, by appling same diagonal reinforcement, two specimens were created by adjusting stirrup of 75%, 50%. So, a total of four specimens were produced. When coupling beam was placed concrete, this experiment was mixed in a content of steel fiber 1%. All the specimens were produced by aspect ratio 3.5(l/h=1050/300) to a half-scale. In this result, two specimens as reduced to stirrup of 75%, 50% was no significant difference in the strength, stiffness and energy dissipation capacity, respectively compared to the stirrup of 100%.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.5
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• pp.587-594
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• 2000

At present embryonic stem (ES) cells with confirmed pluripotential properties are only available in the mouse. Recently, we were able to isolate, culture and genetically transform primordial germ cell (PGC)-derived cells from pig embryos and demonstrate their ability to contribute to chimera development in the pig. In order to determine whether the system we developed could be used to isolate embryonic germ (EG) cells from other mammalian species, we placed isolated PGCs from cattle, goats, rabbits and rats in culture. Briefly, PGCs were isolated from fetuses of cow (day 30-50), goat (day 25), rabbit (day 15-18) and rat (day 11-12), and plated on STO feeder cells in Dulbecco's modified Eagle's medium (DMEM): Ham's F10 medium (1:1) supplemented with 0.01 mM nonessential amino acids, 2 mM L-glutamine, 0.1 mM $\beta$ - mercaptoethnol, soluble recombinant human stem cell factor (SCF; 40ng/ml), human basic fibroblast growth factor (bFGF; 20ng/ml) and human leukemia inhibitory factor (LIF; 20ng/ml). For maintenance of the cells, colonies were passed to fresh feeders every 7-10 days. In all species tested, we were able to obtain and maintain colonies with ES-like morphology. Their developmental potential was tested by alkaline phosphatase (AP) staining and in vitro differentiation assay. For genetic transformation, cells were electroporated with a construct containing the green fluorescent protein (GFP) under the control of the cytomegalovirus (CMV) promoter. GFP-expressing colonies were detected in cattle, rabbits and rats. These results suggest that PGC-derived cells from cattle, goats, rabbits and rats can be isolated, cultured, and genetically transformed, and provide the basis for analyzing their developmental potential and their possible use for the precise genetic modification of these species.