• Journal of Plant Biotechnology
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• v.40 no.3
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• pp.169-177
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• 2013

Recently, the number of people with diabetes is rapidly increasing, coupled with the fact that the insulin market is remarkably increasing. Therefore, molecular farming for plant-derived pharmaceutical protein production is reported as becoming more attractive than ever. In this study, we carried out experiments step by step for development of recombinant insulin constructs, which were transformed into E. coli system, in vitro transcription and translation system, and tobacco cells. At first, recombinant proinsulin protein was successfully produced in in vitro transcription and translation system with wheat germ extract. After which, recombinant construct of prominiinsulin encoded a fusion protein of 7.8 kDa with trypsin cleavage sites at N terminus and C terminus of minimized C-peptide was tried to in vitro expression using E.coli culture. After purification with His-tag column, the resulting recombinant prominiinsulin protein was processed with trypsin, and then checked insulin biosynthesis by SDS-PAGE and western blot analysis with anti-insulin monoclonal antibody. The immunoreactive product of trypsin-treated miniinsulin was identical to the predicted insulin hexamer. The construct of 35S promoter-driven preprominiinsulin recombinant gene with signal peptide region for ER-targeting and red fluorescence protein gene [N terminus ${\rightarrow}$ tobacco E2 signal peptide ${\rightarrow}$ B-peptide (1-29 AA) ${\rightarrow}$ AAK ${\rightarrow}$ A-peptide (1-21 AA) ${\rightarrow}$ RR ${\rightarrow}$ His6 ${\rightarrow}$ KDEL ${\rightarrow}$ C terminus] was transformed into BY-2 tobacco cells. A polypeptide corresponding to the 38-kDa molecular mass predicted for fusion protein was detected in total protein profiles from transgenic BY-2 cells by western analysis. Therefore, this recombinant preprominiinsulin construct can be used for generation of transgenic tobacco plants producing therapeutic recombinant insulin.

• The Korean Journal of Zoology
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• v.38 no.3
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• pp.340-347
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• 1995

We have used two kinds of P element constructs, Pc[(ry+)B] and p[(ry+)$\Delta$SX9], for genetic transformation by microinjection of D. melanogaster. Pc[(ry+)B] construct carrying the rosy gene within an autonomous P element was injected into a true M strain caring the ry506. mutation. The source of transposase for microinjection and transformation was provided by a P element helper plasmid designated p-$\Delta$2-3hs$\pi$, which was co-injected with nonautonomous P[(ry+)$\Delta$SX9] construct into same ry506 M strains. A dechorination method was adopted and 35 independent transformed lines were obtained froin 1143 G0 Injected (35/1143). About 20% of the injected embryos eclosed as adults. Among G0 eclosed flies, approximately 40% exhibited eye color that was similar to wild-type (ry+), but about 60% of fertile G0 transformed lines appeared to have no G1 transformants. Therefore it is unlikely that G0 expression requires integration of the rosy transposon into chromosomes. Pc[(ry+)B] and P[(ry+)$\Delta$SX9] constructs were found to be nearly same in the frequency of element-mediated transformation. On the basis of these results, nonautonomous P elements constructs could he used as same effective vectors in P element-mediated transformation for introducing and fixing genes in insect populations.

• Journal of Korean Elementary Science Education
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• v.35 no.3
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• pp.326-335
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• 2016

The purpose of this study is to devise the concept of properties of the light on learning progressions for 3-6 grade students in the elementary school. For the purpose, this study creates a construct map of the properties of the light. Ordered multiple-choice items were developed in view of the construct map and presented to 200, 3~6 grade students in the elementary school. A partial credit model of Rasch model was used to analyze the results of those items, and the learning progressions was devised from the analysis results. The study can be summarized as follows: the construct modeling approach was used to devise the properties of light on learning progressions. As a result, the concept was selected for the core standards of construct map. Based on the construct map, the multiple-choice items were developed for students' conceptual understanding. The items were analyzed using the partial credit model, and the analysis findings showed that they were appropriate to assess students about their level of understanding of the properties of light. Finally, learning progressions were devised with the use of item analysis results.

• Journal of Korean language education
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• v.29 no.4
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• pp.1-29
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• 2018

This study aims to develop the constructs for evaluating team presentations performed by undergraduate international students in Korea. To achieve the goal, this study reviews related research and sets the criteria to analyze the problems of team presentations performed in Korean by undergraduate international students. Through this analysis we got to know which problems they were making. They were failing to reorganize the resources they had referenced and to organize the contents into one. And errors or mistakes of grammar and spelling were found often in their PPT files, and the editing styles of the PPTs were not consistent. Moreover, their performances were not unlike their PPT files. One made a good presentation but the other students just recited the script without interaction with the audience. Moreover, they could not operate the Q&A part and lead the discussion well, and components for wrapping up the presentation also were omitted frequently. After the analysis, this study conducted a survey with 23 Korean language educators asking about the validity of the construct and description of the survey. Based on the collected feedback, the construct list has been modified primarily, and carried out a second survey with an expert group. Based on their opinion, the final construct list was fine-tuned.

• 대한예방의학회:학술대회논문집
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• 1994.02b
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• pp.137-141
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• 1994

The purpose of this paper is to examine what is meant by a ralid measure of health. Guyatt, Kirshner and Jaeschke propose that health tests should be designed so as to have one of several kinds of validity: 'longitudinal construct validity' for those which are used for longitudinal research designs, and 'cross-sectional construct validity' for those which are used for cross-sectional designs. Williams and Naylor argue that this approach to test classification and validation confuses what a test purports to measure with the purpose for which it is used, and that some tests have multiple uses. A review of the meanings of validity in the psychologica test literature shows that both sets of authors use the term validity in an idiosyncratic way. Although the use of a test (evaluated by content validity) should not be conflated with whether the test actually measures a specified construct (evaluated by construct validity);' if health is actually made up of several constructs (as suggested in Hyland's interactional model) then there may be an association between types of construct and types of purpose. Evidence is reviewed that people make several, independent judgements about their health: cognitive perceptions of health problems are likely to be more sensitive to change in a longitudinal research design. whereas emotional evaluations of health provide less bias in cross-sectional designs. Thus. a classification of health measures in terms of the purpose of the test may parallel a classification in terms of what tests purport to measure.

• Research in Mathematical Education
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• v.18 no.1
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• pp.75-87
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• 2014

It is central to use a scale to measure a person's level of a construct in mathematics education research. This article explains a practical process through which a researcher rapidly can develop an instrument to measure the construct. The process includes research questioning, reviewing the literature, framing a background theory, treating the data, and reviewing the instrument. The statistical treatment of data includes normality analysis, item-total correlation analysis, reliability analysis, and factor analysis. A virtual example is given for better understanding of the process.

• IE interfaces
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• v.9 no.1
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• pp.73-82
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• 1996

The purpose of this research is to construct real-time image acquisition system for a line-scan inspection system as a computer vision system. To construct the line-scan inspection system, many problem arise. However, problems of image acquisitions using linear CCD are practically studied in this paper. These problems are concerned to proper lighting systems, relations with handling system, camera systems, and impacts due to the noise of environment. These problems are examined separately, and proper configurations for each case are suggested to construct the line-scan image acquisition system.

• English Language & Literature Teaching
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• v.11 no.4
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• pp.1-20
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• 2005

Despite the noteworthy increase in the number of FL anxiety studies, inconsistencies associated with the effects of FL anxiety on language learner performance have been reported in literature. Such conflicting results seem to be attributable in part to unstable conceptualization of the FL anxiety construct and its measure. This paper purported to address the emerging call for a theoretical clarification of the construct at hand as a preface to a clear picture of language anxiety on a conceptual ground. This paper not only covers aspects of general anxiety from psychological perspectives, but examines how FL anxiety and its associated concepts have been conceptualized in the literature. Inconsistent results that pertain to FL learning were also delineated. Given the drawbacks found in the exiting theories of FL anxiety, several points were taken into account for a refinement of the conceptual framework. This attempt will hopefully shed new light on the construct per se and prove conducive to the development of the field of English education.

• Proceedings of the IEEK Conference
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• 2002.07b
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• pp.1101-1104
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• 2002

In order to send the information certainly via the network against the packet lost caused by hardware troubles or limitation of packet transferring, we must construct reliable network infrastructure. However, it is difficult to construct comfortable network early if we construct rely on the prediction or the experience through a lot of troubles. In this paper, we propose the method to construct reliable network infrastructure based on the computer network simulation. This simulation is based on the percolation model. Percolation model is known as the model that represents connections. We gave some simulations for the various network topologies: the square lattice network, the cubic lattice network, and the full connection type network.

• 한국컴퓨터산업교육학회:학술대회논문집
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• 2003.11a
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• pp.119-126
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• 2003

Computer simulation has used to a area of military training from about several years ago. War game model(or computer simulation) endow a military man with field training such as combat experience without operating combat strength or capabilities. To samely construct simulation environment against actual combat environment is to well construct DB to operate war game model, associate among federates on network. Thus, we construct virtual combat environment enabling to efficiently manage network traffic among federates(or active nodes) on active network that construct virtual military training space such as urgent combat field needed to rapidly transfer combat information including image and video.