• ALGAE
• /
• 제31권2호
• /
• pp.105-115
• /
• 2016

The genus Pterosiphonia includes twenty-one currently described species of red algae that occur in temperate to tropical regions of the Atlantic and Pacific Oceans. Pterosiphonia spinifera was originally described as Polysiphonia spinifera from Peru and later transferred to Pterosiphonia. Pterosiphonia spinifera has been reported from Peru as Pterosiphonia pennata, which was originally described from the Mediterranean Sea. Recently, Pterosiphonia arenosa was described based on specimens of P. pennata from Korea. We collected P. spinifera along the coast of Peru and P. arenosa near the type locality in Korea. We compared them with the isotype specimens of P. arenosa using both morphological and molecular data. Our morphological observations and our phylogenetic analysis of rbcL sequences demonstrate that P. spinifera and P. arenosa are conspecific and indicate that P. arenosa is a later synonym of P. spinifera. Our study confirms the wide occurrence of P. spinifera in the western and eastern Pacific Ocean.

• Animal Systematics, Evolution and Diversity
• /
• 제30권3호
• /
• pp.206-214
• /
• 2014

Two monstrilloid copepod species belonging to the genus Monstrilla Dana are newly recorded from Korea: M. grandis Giesbrecht, 1891 and M. hamatapex Grygier and Ohtsuka, 1995. Specimens were obtained from inshore waters around the coasts of South Korea, using a light trap installed at quays and wharves overnight. Based on the Korean specimens, the two species are redescribed, with brief accounts of their affinities and morphological variabilities. Remarkable morphological discrepancies are confirmed in female leg 5 of M. grandis between type material from Southern Hemisphere and European and Asian specimens, which raise a strong doubt on their conspecificity. Korean specimens of M. hamatapex well coincide with the type specimens from Japan, except for some variations in the setal armature of leg 5 and antennules. This is the second taxonomic paper on the monstrilloid copepods in Korea, and the genus Monstrilla is newly added to Korean fauna.

• Mycobiology
• /
• 제46권1호
• /
• pp.1-12
• /
• 2018

Lactarius and Lactifluus are milkcaps that are characterized by the secretion of latex. These two genera are part of a globally distributed cosmopolitan group of ectomycorrhizal fungi that is an important food resource in various ecosystems. Recently, the taxonomy of Lactarius and Lactifluus has been revised based on molecular phylogenetics. Despite the importance of these taxa, Korean species of both genera are poorly understood. In an effort to describe milkcap species that are indigenous to Korea, a long-term study has been initiated. During a recent survey, eight species of milkcaps that were previously unrecorded in Korea were detected based on morphological observation and sequence analysis of the internal transcribed spacer region: five Lactarius species (Lactarius atromarginatus, L. austrotorminosus, L. kesiyae, L. tabidus, and L. vietus) and three Lactifluus species (Lactifluus acicularis, Lf. pilosus, and Lf. pinguis). Detailed morphological descriptions and phylogenetic relationships of these species are provided in this article.

• ALGAE
• /
• 제38권1호
• /
• pp.23-38
• /
• 2023

Accurately identifying species is the basis of all biological studies. There has been much confusion in the identification of Grateloupiacean species, which have finely pinnate gross morphology similar to Grateloupia filicina (the type species of the family). The objective of this study was to comprehensively investigate species identification and distribution of G. filicina-like species in the Northwest Pacific, based on the rbcL sequences. A total of 118 specimens from 78 sites in Korea and Japan were collected from 2001 to 2021 and analyzed for their rbcL sequences. Additional 341 sequences downloaded from the GenBank were included in our comprehensive dataset. Based on these sequences, we documented the nomenclatural history and geographical distribution of the species, and commented on the application of species name. G. asiatica was the most abundant G. filicina-like species in the Northwest Pacific, and its high degree of morphological variation caused many misidentifications. In particular, G. dalianensis, G. serra, and G. variata require reconsideration of their conspecificity with G. asiatica using more specimens from China. By contrast, G. oligoclora was presumed to be a heterotypic synonym of G. subpectinata. The occurrence of G. acuminata, G. ramosissima, and G. livida in Korea resulted from misidentifications with other species.

• Journal of Ginseng Research
• /
• 제40권2호
• /
• pp.176-184
• /
• 2016

Background: Wild ginseng, Panax ginseng Meyer, is an endangered species of medicinal plants. In the present study, we analyzed variations within the ribosomal DNA (rDNA) cluster to gain insight into the genetic diversity of the Oriental ginseng, P. ginseng, at artificial plant cultivation. Methods: The roots of wild P. ginseng plants were sampled from a nonprotected natural population of the Russian Far East. The slides were prepared from leaf tissues using the squash technique for cytogenetic analysis. The 18S rDNA sequences were cloned and sequenced. The distribution of nucleotide diversity, recombination events, and interspecific phylogenies for the total 18S rDNA sequence data set was also examined. Results: In mesophyll cells, mononucleolar nuclei were estimated to be dominant (75.7%), while the remaining nuclei contained two to four nucleoli. Among the analyzed 18S rDNA clones, 20% were identical to the 18S rDNA sequence of P. ginseng from Japan, and other clones differed in one to six substitutions. The nucleotide polymorphism was more expressed at the positions 440-640 bp, and distributed in variable regions, expansion segments, and conservative elements of core structure. The phylogenetic analysis confirmed conspecificity of ginseng plants cultivated in different regions, with two fixed mutations between P. ginseng and other species. Conclusion: This study identified the evidences of the intragenomic nucleotide polymorphism in the 18S rDNA sequences of P. ginseng. These data suggest that, in cultivated plants, the observed genome instability may influence the synthesis of biologically active compounds, which are widely used in traditional medicine.

• 한국균학회지
• /
• 제27권5호
• /
• pp.322-327
• /
• 1999

영지버섯 원목재배시 발생한 백색자실체에서 2핵 균사체 5균주를 분리하였다. 이들 균주의 자실체는 정상적인 갓, 자실층, 대로 분화되지 않았고, 발생해서 3개월 후에도 착색되지 않았다. 이들 백색자실체 형성균주와 재배용 균주는 배지상에서의 생장률, 균총의 색 그리고 비정형자실체(atypical fruiting structure; AFS) 형성에 차이를 보였다. 백색자실체 형성균주는 환기와 광 조건하에서 갈색의 AFS를 쉽게 형성하였다. Gl-010과 Gl-011 균주는 암상태와 BLB 광을 조사하였을 때에는 AFS를 형성하지 않았지만, 가시광선하에서는 AES를 형성하였다. 이들 균주의 AFS형성은 $0.5{\mu}mol\;m^{-2}\;s^{-1}$ 이하의 약한 광에서 잘 형성되었고, 그 이상의 광도에서는 억제를 받았다. 한편, Gl-010과 Gl-011 균주 이외의 4 균주는 암상태와 BLB에서도 AFS를 잘 형성하였으나, 이들의 모균주인 Gl-030은 어때한 광 조건하에서도 AFS를 형성하지 못하였다. AFS에서 형성된 담자포자를 발아시켜 얻은 단핵 균사체는 모균주와 화합성이었다.