• Title/Summary/Keyword: Conjugate Method

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Rapid diagnosis of Pseudotuberclosis in yellowtail (Seriola quinqueradiata) by immunofluorescent antibody technique (면역형광항체법(免疫螢光抗體法)에 의(依)한 방어의 유결절증(類結節症) 신속(迅速) 진단(診斷))

  • Bang, Jong-Deuk;Jung, Sung-Hee;Chun, Seh-Kyu
    • Journal of fish pathology
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    • v.3 no.1
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    • pp.11-19
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    • 1990
  • Yellow tail (Seriola quinqueradiata) infected by Pasteurella piscicida have been occurred to mass mortality without showing apparent surface lesions in cage culture farms. In this case, it is necessary to consider countermeasure by rapid diagnosis of infected fish. The purpose of the present study was to investigate usefulness of the direct fluorescent antibody technique(FAT) for rapid diagnosis of pseudotuberclosis of cultured yellowtail caused by P. piscicida. Antibody produced by inoculating rabbit with formalin killed pseudotuberclosis bacteria antigen(strain KNP-2). Immunoglobulin-G(IgG) was purified from antisera by DEAE-cellulose column chromatography and conjugate with fluorescein isothiocyanate. Fluorescein-labeled antisera was purified by sephadex G-25 gel column chromatography. The fluorescein/protein molar ratio of labeled antisera was determined as 8.8-9.5. Diagnosis of cultured yellowtail was examined in cage culture farms which located in Tongyung, kyungnam from July to October 1990. The causative bacteria of pseudotuberclosis could be detected within two hours after the specimens were transferred to the laboratory for FAT, and it showed that FAT could be adapted as a rapid and accurate diagnostic method of pseudotuberclosis in yellowtail.

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Matching Method of Digital Map and POI for Geospatial Web Platform (공간정보 플랫폼 구축을 위한 전자지도와 POI 정보의 매칭 방법)

  • Kim, Jung-Ok;Huh, Yong;Lee, Won-Hee;Yu, Ki-Yun
    • Journal of Korean Society for Geospatial Information Science
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    • v.17 no.4
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    • pp.23-29
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    • 2009
  • Recent growth of the geospatial information on the Web has made it possible to easily access a wide variety of geospatial information. An integration of different geospatial objects consists of the following three steps; extracting geospatial objects from the maps, converting the coordinate system and discovering pairs of objects that represent the same real-world entity in the two maps. This paper deals mainly with the third step to correspond conjugate objects and four matching types and criteria is presented. The techniques designed and developed can be utilized to efficiently integrate distributed heterogeneous spatial databases such as the digital maps and POIs from other data sources. To achieve the goal, we presented four types and criteria for the matching schema. The main contributions of this paper are as follows. A complete process of integrating data from maps on the Web is presented. Then, we show how attributes of the objects can be used in the integration process.

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Implementation of Stable Optical Information Security System using Interference-based Computer Generated Hologram iud $BaTiO_3$ (간섭을 기반으로한 컴퓨터형성홀로그램과 $BaTiO_3$를 이용한 안정한 광 정보보호 시스템의 구현)

  • 김철수;김종윤;박영호;김수중;조창섭
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.28 no.8C
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    • pp.827-834
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    • 2003
  • In this paper, we implemented an optical information security system using computer generated hologram based on the principle of interference and BaTiO$_3$that is photorefractive material. First of all, we would generate binary phase hologram which can reconstruct the original image perfectly, and regard this hologram as the image to be encrypted. And then applying the interference rule to the hologram, encrypted and reference (fkey information) images are generated. In the decrypting process, we can get an interference intensity by interfering the reference image and the encrypted image in the Mach-Zehnder interferometer. and transforming interference intensity information into phase information using LCD(liquid crystal display) and finally recover original image by inverse Fourier transforming the phase information. In this process, the Intensity information generated by interference of two images is very sensitive to external vibrations. So, we get a stable interference using the characteristic of SPPCM(self pumped phase conjugate mirror) of BaTiO$_3$that is photorefractive material. The proposed method has an advantage of double image encryption by encrypting the hologram of the image instead of original image.

A GPU-enabled Face Detection System in the Hadoop Platform Considering Big Data for Images (이미지 빅데이터를 고려한 하둡 플랫폼 환경에서 GPU 기반의 얼굴 검출 시스템)

  • Bae, Yuseok;Park, Jongyoul
    • KIISE Transactions on Computing Practices
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    • v.22 no.1
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    • pp.20-25
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    • 2016
  • With the advent of the era of digital big data, the Hadoop platform has become widely used in various fields. However, the Hadoop MapReduce framework suffers from problems related to the increase of the name node's main memory and map tasks for the processing of large number of small files. In addition, a method for running C++-based tasks in the MapReduce framework is required in order to conjugate GPUs supporting hardware-based data parallelism in the MapReduce framework. Therefore, in this paper, we present a face detection system that generates a sequence file for images to process big data for images in the Hadoop platform. The system also deals with tasks for GPU-based face detection in the MapReduce framework using Hadoop Pipes. We demonstrate a performance increase of around 6.8-fold as compared to a single CPU process.

Lateral Stiffness of Steel Moment Frames Having Dogbone Seismic Connection (독본(dogbone) 내진접합부를 갖는 철골 모멘트골조의 횡강성 평가)

  • 이철호
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.15 no.4
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    • pp.639-647
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    • 2002
  • A simplified analytical procedure is presented to estimate the reduction of elastic lateral stiffness of steel moment frames arising from the radius-cut dogbone weakening. With the original radius-cut dogbone shape, it is almost impossible or too complicated to integrate analytically the mathematical expressions encountered when applying the conjugate beam method to compute the beam deflection component. In this study, the problem is circumvented by replacing the original radius-cut dogbone with an equivalent dogbone of constant width. The equivalence between the two is established by imposing an equal dogbone elongation criterion. This approach is justified by using a calibrated finite clement analysis. Then, the elastic lateral deflection components from the column, panel zone, and beam are derived for a typical beam-column subassembly. The derived results can be used to evaluate the reduction of the frame lateral stiffness. Case studies conducted within some practical ranges of frame configurations show that the reduction in frame lateral stiffness due to the presence of dogbone cut is on the order of 1 to 2 percent and is reasonably negligible in practical sense.

Linear Tapered Slot Rectifying Antenna for Portable UHF-Band RFID System (휴대용 UHF대역 RFID 시스템을 위한 선형 테이퍼드 슬롯 정류 안테나)

  • Pyo, Seongmin
    • Journal of IKEEE
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    • v.24 no.1
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    • pp.368-371
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    • 2020
  • In this paper, we propose a linear tapered slot rectifying antenna for a portable UHF-band RFID system. Since the proposed rectifying antenna does not use a dielectric substrate, the planar antenna is implemented with a thin metal thickness. The rectifier circuit converts input RF power into output DC voltage using a voltage doubler circuit based on two anti-parallel schottky diodes. The rectifying antenna is integrated by the voltage doubler circuit into a linear tapered slot antenna. For conjugate impedance matching of the rectifying circuit and the linear tapered slot antenna, the source-pull method was utilized by adjusting the angle of the tapered slot and the length of the antenna feed line. The proposed antenna prototype has been verified with the electrical and radiation characteristics through RF-DC conversion and far-field radiation test in open space measurement environment. Finally, the proposed antenna is realized to 0.23-wavelength (75 mm) and 0.18-wavelength (60 mm) at 915 MHz center frequency.

Non-Invasive Colorimetric Magneto Loop-Mediated Isothermal Amplification (CM-LAMP) Method for Helicobacter pylori Detection

  • Bangpanwimon, Khotchawan;Mittraparp-arthorn, Pimonsri;Srinitiwarawong, Kanchana;Tansila, Natta
    • Journal of Microbiology and Biotechnology
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    • v.31 no.4
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    • pp.501-509
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    • 2021
  • More than half the world's population is thought to be infected with Helicobacter pylori. Although the majority of infected people are asymptomatic, H. pylori infection may cause gastric ulcers and deadly gastric cancer. Owing to the difficulty and invasiveness of current routine culture and diagnostic methods, a highly sensitive and specific noninvasive assay for H. pylori is of interest. This study highlighted the design and performance of a colorimetric magneto loop-mediated isothermal amplification (CM-LAMP) assay to detect H. pylori in spiked saliva samples. LF primers were coated on magnetic nanoparticles by carbodiimide-induced immobilization and functionally used for solid-phase amplification. During the LAMP reaction at 66℃, biotin-tagged FIPs were incorporated into LAMP amplicons. The colorimetric signal developed after the addition of NeutrAvidin horseradish peroxidase conjugate (NA-HRP) and ABTS. None of the tested microorganisms, including closely related bacteria, was shown positive by the CM-LAMP assay except H. pylori isolates. This novel platform was highly specific and 100-fold more sensitive (40 CFU/ml or 0.2 CFU per reaction) than the PCR and conventional LAMP assays for the detection of H. pylori in spiked saliva. Our results demonstrated the feasibility of using this noninvasive molecular diagnostic test to detect H. pylori in saliva samples.

Building Detection Using Edge and Color Information of Color Imagery (컬러영상의 경계정보와 색상정보를 활용한 동일건물인식)

  • Park, Choung Hwan;Sohn, Hong Gyoo
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.26 no.3D
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    • pp.519-525
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    • 2006
  • The traditional area-based matching or efficient matching methods using epipolar geometry and height restriction of stereo images, which have a confined search space for image matching, have still some disadvantages such as mismatching and timeconsuming, especially in the dense metropolitan city that very high and similar buildings exist. To solve these problems, a new image matching method through building recognition has been presented. This paper described building recognition in color stereo images using edge and color information as a elementary study of new matching scheme. We introduce the modified Hausdorff distance for using edge information, and the modified color indexing with 3-D RGB histogram for using color information. Color information or edge information alone is not enough to find conjugate building pairs. For edge information only, building recognition rate shows 46.5%, for color information only, 7.1%. However, building recognition rate distinctly increase 78.5% when both information are combined.

Comparative Study of Near-Wall Treatment Methods for Prediction of Heat Transfer over Gas Turbine Nozzle Guide Vane (가스터빈 노즐 베인의 열전달 예측을 위한 벽면처리법 비교연구)

  • Bak, Jeonggyu;Kim, Jinuk;Lee, Seawook;Gang, Youngseok;Cho, Leesang;Cho, Jinsoo
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.38 no.7
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    • pp.639-646
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    • 2014
  • The comparative analysis of near-wall treatment methods that affect the prediction of heat transfer over the gas turbine nozzle guide vane were presented. To achieve this objective, wall-function and low Reynolds number methods, and the transition model were applied and simulated using NASA's C3X turbine vane. The predicted turbine vane surface pressure distribution data using the near-wall treatment methods were found to be in close agreement with experimental data. However, the predicted vane metal temperature and heat transfer coefficient displayed significant differences. Overall, the low Reynolds method and transition model did not offer specific advantages in the prediction of temperature and heat transfer than did the wall-function method. The Reynolds stress model used along with the wall-function method resulted in a relatively high accuracy of prediction of the vane metal temperature and heat transfer coefficient.

Studies on the Biological Behaviors of Taxol Derivatives (Taxol 유도체들의 생물학적 거동에 관한 연구)

  • Awh, Ok-Doo;Yoo, Dae-Wung;Im, Sang-Moo
    • The Korean Journal of Nuclear Medicine
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    • v.31 no.4
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    • pp.440-451
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    • 1997
  • This study was designed to prospect the $^{111}In$-labelled paclitaxel as tumor imaging agent. In order to provide a taxol molecule with a functional group which is able to chelate In-111, taxol-DTPA conjugate and 2'-hemisuccinyltaxol were synthesized by esterification of taxol at C-2'on C-13 carbon with DTPA anhydride and succinic anhydride, respectively. Synthesis yield of the taxol derivatives was 34% for taxol-DTPA and 80% for 2'-hemisuccinyltaxol. Cytotoxicity of the taxol derivatives were measured by MTT method toward cell lines HT29, B16, P388, and CT26. The cytotoxic activities of the taxol derivatives were maintained, although less active than taxol. Radiolabelling of the taxol derivatives were proceeded directly with $^{111}InCl_3$ or indirectly with $^{111}In$-citrate(ligand-exchange method). The ligand-exchange method was not suitable because some precipitates appeared during the reaction. On the contrary, by direct radiolabelling method, we were able to obtain taxol-DTPA-$^{111}In$ in 100% radiochemical yield. However, 2'-hemisuccinyltaxol was not labelled by both methods. Yield and radiochemical purity of the radiolabelled com-pound were determined by HPLC, paper chromatography and instant thin layer chromatography. Taxol-DTPA-$^{111}In$ was characterized to be hydrophilic by lipophilicity test, and nearly non-adhesive to HT29, B16, P388, and CT26 by cell binding affinity test. Binding affinity of the taxol-DTPA-$^{111}In$ complex to serum proteins was also examined by protein precipitation with 30% trichloroacetic acid. The results showed that 30% of the taxol-DTPA-$^{111}In$ complex binds with serum proteins.

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