• 한국정밀공학회지
• /
• 제27권9호
• /
• pp.67-77
• /
• 2010

An internal lobe pump is suitable for oil hydraulics of machine tools, automotive engines, compressors, constructions and other various applications. In particular, the pump is an essential machine element of an automotive engine to feed lubricant oil. The subject of this paper is the theoretical analysis of internal lobe pump whose the main components are the rotors: usually the outer one is characterized by lobe with elliptical and involute shapes, while the inner rotor profile is determined as conjugate to the other. And the integrated design system which is composed of three main modules has been developed through AutoLISP under AutoCAD circumstance plus CFD-ACE+. It generates new lobe profile and calculates automatically the flow rate and flow rate irregularity according to the lobe profile generated. CFD simulation results show trends similar to those carried out in experiments, and a quantitative comparison is presented. Results obtained from the automotive integrated design system enable the designer and manufacturer of oil pump to be more efficient in this field.

• 한국통신학회논문지
• /
• 제25권1B호
• /
• pp.89-98
• /
• 2000

본 논문에서는 적응필터를 기반으로 한 웨이브릿 변환 및 서브밴드 필터뱅크를 사용한 능동잡음제거의 모델을 제안한다. 분해 필터뱅크는 입력 및 오차신호를 저주파 및 고주파영역의 QMF 필터뱅크로 분해하며, 각 필터뱅크 에는 dyadic tree 구조를 갖는 웨이브릿 필터를 사용한다. 분해된 입력 및 오차신호는 filtered-X LMS 알고리듬를 사용하여 각 서브밴드의 적응 필터계수를 새롭게 갱신시킨다. 합성 필터뱅크는 그리고 각 서브밴드의 적응필터 출력신호를 합성한 후 완전복원이 되는 광대역의 출력신호를 만든다. 분해 및 합성 필터뱅크는 완전복원을 위하여 공액직교필터를 사용한다. 또한 오차경로의 전달특성을 온라인 추정하기 위한 지연 LMS 알고리듬 모델은 이득과 시간지연인자만을 사용한다. 따라서 제안한 적응 능동잡음제거 모델은 웨이브릿 서브밴드 필터뱅크를 사용하여 계산량과 수렴속도에 유리한 시스템이 되도록 제시한다.

• 대한기계학회논문집B
• /
• 제34권5호
• /
• pp.553-561
• /
• 2010

한국원자력연구원에서는 초고온가스로를 모사할 수 있는 중형 헬륨 회로를 건설 중에 있다. 이 실험헬륨 회로에서 두 개의 전기 가열기가 헬륨 유체를 1 ~ 9 MPa 의 압력 하에서 $950^{\circ}C$ 까지 가열하게 된다. 이 실험 헬륨 회로의 설계 사양을 최적화하기 위하여, 본 연구에서는 두 개의 가열기 중 하류에 위치한 고온헬륨가열기 안의 복합열전달 현상을 전산유체역학으로 해석하였다. 해석 결과에서 헬륨 가열기 내 최대 온도는 허용 한계를 넘지 않았고, 이로써 선정된 기하구조의 열적 특성은 설계요건을 만족함이 확인되었다.

• 대한기계학회논문집A
• /
• 제40권7호
• /
• pp.653-658
• /
• 2016

가스터빈엔진의 고온 부품은 초내열 합금 재료를 이용하며, 냉각설계 적용으로 형상이 복잡하여 정밀 주조 과정을 거치게 된다. 터빈 부품에 주로 적용이 되는 일방향 응고 및 단결정 재료는 제조 과정에서 결정립 성장 방향이 설계와 다르게 섭동을 가지게 되며, 이는 각 방향에 대한 재료 상수의 섭동을 유발하여 응력 분포의 변화와 함께 피로 수명에 큰 산포를 야기하게 된다. 본 연구에서는 일방향 응고 재료 노즐에 대하여 결정립 성장 방향의 섭동에 대한 구조 건전성의 영향을 저주기 피로 수명을 통해 확인하여, 향후 제작 허용값에 대한 제안 및 좀 더 정교한 통계적 접근이 필요함을 확인하고자 한다. 이를 위해 복합 열전달 해석을 통해 금속 온도 분포를 계산하고 이를 근거로 구조 해석 및 저주기 피로 수명을 계산하였다.

• 한국측량학회지
• /
• 제24권5호
• /
• pp.409-416
• /
• 2006

영상 기하보정은 여러 가지 데이터의 조합으로부터 얻어질 수 있는 영상 분석 작업에 매우 중요한 전처리 과정 중 하나다. 본 연구는 최근 평면 영상간 기하보정에 많이 사용하고 있는 SIFT 기법을 적용하여, 다양한 해상도를 가진 위성영상의 자동 기하보정을 수행하는데 초점을 맞추고 있다. 2차원 영상의 조건을 만족하기 위해 기복변위의 영향이 상대적으로 적은 중 저해상도 위성영상의 기하보정을 수행하였으며, 다양한 해상도 영상을 조합함으로써 공간해상도의 차이를 보이는 영상의 기하보정에 SIFT 기법을 적용할 수 있는지를 실험하였다. 결과를 통해, SIFT 기법이 기존의 상관계수를 이용하여 특징점의 정합을 수행하는 방법에 비해 높은 정합률을 나타냈으며, 추출된 정합쌍을 자동 영상 기하보정에 사용할 수 있을 것으로 기대한다.

• The Plant Pathology Journal
• /
• 제23권4호
• /
• pp.260-265
• /
• 2007

Testing a large number of samples from field monitoring and routine indexing is cumbersome and the available virus detection tools were labor intensive and expensive. To circumvent these problems we established tissue blot immunoassay (TBIA) method an alternative detection tool to detect Barley mild mosaic virus (BaMMV) and Barley yellow mosaic virus (BaYMV) infection in the field and greenhouse inoculated plants for monitoring and routine indexing applications, respectively. Initially, leaf and stem were tested to determine suitable plant tissue for direct blotting on nitrocellulose membrane. The dilutions of antibodies were optimized for more efficient and economical purposes. Results showed that stem tissue was more suitable for direct blotting for it had no background that interferes in the reaction. Therefore, this technique was referred as direct stem blot immunoassay or DSBIA, in this study. Re-used diluted (1:1000) antiserum and conjugate up to 3 times with the addition of half strength amount of concentrated antibodies was more effective in detecting the virus. The virus blotted on the nitrocellulose membrane from stem tissues kept at room temperature for 3 days were still detectable. The efficiency of DSBIA and RT-PCR in detecting BaMMV and BaYMV were relatively comparable. Results further proved that DSBIA is a rapid, reliable and economical detection method suitable for monitoring BaMMV and BaYMV infection in the field and practical method in indexing large scale of barley materials for virus resistance screening.

• Toxicological Research
• /
• 제8권2호
• /
• pp.255-263
• /
• 1992

SKI 2053R and SKI 2053R-human serum albumin(HSA) mixture were examined for their antigenicity in Hartley guinea pigs as well as C57BL/6 mice in comparison with distilled water (DW), HSA and DW-HSA conjugate. Several antigenicity tests, including acitive systemic anaphylaxis(ASA), passive systemic anaphylaxis (PSA), passive cutaneous anaphylaxis (PCA) and indirect hamagglutination test (IHA), were performed according to the Established Regulations of National Institute of Safety Research. The results were as follows: 1. When guinea pigs were sensitized with SKI2053R or SKI2053R-HSA emulsified with complete Freund's adjuvant(CFA), these animals showed negative reactions in ASA and PSA. 2.No blue spot was observed on the back skin of guinea pigs in the PCA test. 3. Sera from guinea pigs revealed a negative reaction in IHA. 4.Guinea pigs were sensitized by HSA emulsified with CFA as a positive control, and these animals showed positive reactions in ASA, PSA, PCA, and IHA. As shown above, SKI2053R was considered to possess neither antigenic, nor haptenic properties, and confirmed not to have the haemagglutinating activity.

• 대한수의학회지
• /
• 제45권4호
• /
• pp.569-574
• /
• 2005

Enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to reticuloendotheliosis virus (REV) at single serum dilution was standardized. REV HI, one of the Korean field isolates, was inoculated into chicken embryo fibroblast (CEF) cells and was harvested from the culture fluids and cells after 10 to 12 days. Viruses were purified by centrifugation at the $107,000{\times}g$ for 12 hours on 20, 30, 45% (W/V) sucrose gradient. Virus specific fraction was collected and used as ELISA antigen. To standardize ELISA, the optimal concentration of coating antigen ($1{\mu}g/well$) and conjugate (1/1000) was determined by corrected OD (OD value of positive serum-OD value of negative serum) and P/N ratio (OD value of positive serum/OD value of negative serum). To calculate ELISA titer by measuring absorbance at 1/400 single serum dilution, serum titrations were carried out for various sample sera together with standard positive and negative sera. The observed titers of serum samples were plotted against sample/positive (s/p) ratios at 1/400 serum dilution. From the above data, the ELISA titers could be calculated by the equation of $log_{10}$ ELISA titer = 2.2763 ($log_{10}$ s/p) + 3.482 (r = 0.93). For evaluating the sensitivity, the standardized method were compared with conventional agar gel immunodiffusion (AGID) test method using serum samples collected from REV infected field chicken flocks. Fifty seven of 60 samples (95%) were positive for REV by ELISA, whereas only 11 (18.3%) samples were positive by AGID test. This results suggested that the ELISA tests developed in this study could be used for detection of antibodies to REV with high sensitivity.

• 한국잡초학회지
• /
• 제15권4호
• /
• pp.247-253
• /
• 1995

The herbicide butachlor [N-(butoxymethyl)-2-chloro-N-(2,6-di-methylphenyl) acetamide] is widely used by farmers as a tool for weed management of transplanted rice(Oryza sativa L.) in Taiwan. The herbicide did not stop germination of rice and weed seeds, but strongly inhibited the subsequent growth of young shoots and roots. The inhibition was also strong on established seedlings. However, they could recover to normal growth after the herbicide effect disappeared. Butachlor greatly decreased the endogenous indole-3-acetic acid (IAA) but increased the endogenous abscisic acid (ABA) contents of rice seedlings. Addition of lAA into growth medium (Hoagland's solution) partly relieved growth inhibition. Pretreatment of both gibberellic acid ($GA_3$) and IAA 24 hours before butachlor treatment almost completely alleviated the butachlor-interfere with GA and/or IAA metabolism or their action resulting in the growth inhibition of rice. Butachlor was readily absorbed by rice roots. During 24 hours of uptake experiment, 32% of the applied herbicide was absorbed. Pretreatment of the herbicide for 2 days did ncx affect the absorption. Of the absorbed herbicide, 80% remained in roots, only 20% transported into shoots, and more than 50% was metabolized to water soluble substances. Thin-layer chromatographic (TLC) analysis indicated that the Rf value of the most abundant metabolite was butachlor-glutathione conjugate. Rice, barnyardgrass (Echinochloa crus-galli (L.) Beauv.), and monochoria (Monochoria vaginalis Presl) seedlings contained relatively high level of non-protein thiols, while the glutathione S-transferase (GST) activity was found highest in rice, barnyardgrass the next, monochoria the lowest. The difference in GST activity among these species might be related to their sensitivity to butachlor.

• 대한미생물학회지
• /
• 제22권4호
• /
• pp.377-392
• /
• 1987

Monoclonal antibody to the Escherichia coli heat-stable enterotoxin(ST) was produced to develop a rapid and convenient diagnostic method to the ST. The toxin was purified from culture supernatant of enterotoxigenic E. coli O148H28($ST^+/LT^+$) and conjugated to bovine serum albumin(BSA). The ST-BSA conjugate was used to immunize BALB/c mice and the immune spleen cells from these mice were fused with $P3{\times}63$ Ag8.V653 plasmacytoma cells. Hybridomas were screened by ELISA and positive hybridomas were cloned by limiting dilution. Finally, one stable clone (AS36) specific to ST was selected for further growth and characterization. Antibody titers of culture supernatant and ascitic fluid from BALB/c mice were 1:1,024 and 1:20,480 respectively in ELISA. The isotype and subclass of monoclonal antibody was IgG1 in sandwich ELISA. To test the neutralizing effect of monoclonal antibody on toxin activity of ST, mixture of ascitic fluid and ST was assayed by infant mouse assay and this monoclonel antibody was proved to be a neutralizing antibody. The titer of ascitic fluid which completely neutralized biological activity of 4 units of ST was 1:4. Purified ST was quantitatively measured by competitive ELISA and minimum amount of ST detectable by this assay was 250pg, which was an amount six-fold smaller than that detectable by infant mouse assay. Four reference strains of enterotoxigenic E. coli from WHO were detected by competitive ELISA and highly specific, sensitive and reproducible result was obtained.