• The Bulletin of The Korean Astronomical Society
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• v.44 no.2
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• pp.79.1-79.1
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• 2019

The Schwarzchild-Chang telescope is a confocal off-axis two mirror telescope with D = 50 mm, F = 100 mm and FOV = 8 ° × 8 °. Unlike common off-axis telescopes, the mirrors of the Schwarzchild-Chang telescope share their focal points to remove the linear astigmatism. In this poster, we show the alignment process of the Schwarzchild-Chang telescope with wavefront measurement and the sensitivity table method. Wavefront is measured using the Shack-Hartmann sensor, and Zernike polynomials are obtained from measured wavefront. Sensitivity table method is to calculate alignment errors from the Zernike coefficients. As a result, we evaluate tilt, decenter, and despace of each mirror of linear astigmatism-free con-focal off-axis system.

• Journal of Institute of Control, Robotics and Systems
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• v.22 no.2
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• pp.139-146
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• 2016

Recently, popularity of 3D technology has been growing significantly and it has many application parts in the various fields of industry. In order to overcome the limitations of 2D machine vision technologies based on 2D image, we need the 3D measurement technologies. There are many 3D measurement methods as such scanning probe microscope, phase shifting interferometry, confocal scanning microscope, white-light scanning interferometry, and so on. In this paper, we have used the extended depth of focus (EDF) algorithm among 3D measurement methods. The EDF algorithm is the method which extracts the 3D information from 2D images acquired by short range depth camera. In this paper, we propose the EDF algorithm using the edge informations of images and the average values of all pixel on z-axis to improve the performance of conventional method. To verify the performance of the proposed method, we use the various synthetic images made by point spread function(PSF) algorithm. We can correctly make a comparison between the performance of proposed method and conventional one because the depth information of these synthetic images was known. Through the experimental results, the PSNR of the proposed algorithm was improved about 1 ~ 30 dB than conventional method.

• Journal of Microbiology and Biotechnology
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• v.19 no.5
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• pp.495-501
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• 2009

A new spore display method is presented that enables recombinant proteins to be displayed on the surface of Bacillus spores via fusion with InhA, an exosporium component of Bacillus thuringiensis. The green fluorescent protein and $\beta$-galactosidase as model proteins were fused to the C-terminal region of InhA, respectively. The surface expression of the proteins on the spores was confirmed by flow cytometry, confocal laser scanning microscopy, measurement of the enzyme activity, and an immunogold electron microscopy analysis. InhA-mediated anchoring of foreign proteins in the exosporium of Bacillus spores can provide a new method of microbial display, thereby broadening the potential for novel applications of microbial display.

• Proceedings of the Materials Research Society of Korea Conference
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• 2011.05a
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• pp.51.1-51.1
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• 2011

An electrospun Poly (lactice-co-glycolide acid) (PLGA) and Gelatin nanofiber tube was fabricated for potential intestinal stent application. Mechanical properties of tube were evaluated by tensile strength and burst strength tests. Physical and chemical properties were evaluated by contact angle measurement, swelling rates and porosity measurements. Biodegradability was investigated by immersion in simulated body fluid (SBF). Biocompatibility was investigated in vitro by cytotoxicity and proliferation studies by MTT assay, confocal microscopy and western blot using IEC-18 (Rat intestinal epithelial cell). After intestinal stent was implanted into rat bowel for periods from 7 to 10days, it was then analyzed using micro-computed tomography (Micro CT) and X-ray techniques. Futhermore, histological analysis was performed by hematoxylin-eosin (H&E) stain.

• The Bulletin of The Korean Astronomical Society
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• v.42 no.1
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• pp.56.4-57
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• 2017

The Schwarzschild-Chang off-axis telescope is a "linear astigmatism-free" confocal system. The telescope comprises two pieces of aluminum-alloy freeform mirrors that are fabricated with diamond turning machine (DTM) process. We designed optomechanical structures where optical components in the telescope system can be adjustable on a linear stage. Optomechanical deformation caused by the weight of system itself and its temperature variation is analyzed by the finite element analysis (FEA). The results show that the deformation is estimated in the tolerance range. For the optic-axis alignment of telescope system, three-point alignment (TPA) method is chosen. The TPA method uses three parallel lasers and a plane mirror. Point source images were taken from collimated light and field observation. The performance of optical system was tested by point spread function and aberration measurement of the point sources.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.32 no.12
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• pp.1102-1106
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• 2008

Microfluidics deals with the behavior, precise control and manipulation of fluids at a micro scale. It has become increasingly prevalent in various applications such as biomedical applications (diagnostics, therapeutics, and cell/tissue engineering), inkjet head, and fuel cells etc. The issue of inspection and characterization of microfluidics has emerged as a major consideration in design, fabrication, and detection of microfluidic devices. In this paper, we characterize a diffusion based mixing in Y-microchannel using a fluorescent optical scanner based on a DVD pick-up module, which is widely used in optical storages. Using fluorescent dye, we measure the fluorescent intensity that represents the mixing patterns in Y-microchannel. We also compare these experimental results with computational fluid dynamics (CFD) simulation ones. It is shown that the proposed optical scanner can be used as an alternative measurement system with high performance and cost-effectiveness, compared to conventional optical tools such as epifluorescent microscopes using high resolution CCD camera and confocal microscopes with photomultiplier (PMT) detectors.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.30 no.9 s.252
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• pp.834-841
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• 2006

In the present wort the Laser-induced Fluorescence (LIF) technique and Confocal Laser Scanning Microscopy (CLSM) have been combined to measure the temperature distribution across a micro-scale liquid layer as a direct and non-invasive method. Only the fluorescent light emitted from a very thin volume around a focal plane can be selectively detected, and it enables us to measure the liquid temperatures even at the close vicinity of the walls. As an experimental verification, a test section consists of two flat plates (for heating and cooling, respectively) separated by about 240 microns was made, and the methanol mixed with a temperature-sensitive dye, Rhodamine B, was filled in the gap between them. The measured temperature distribution across the gap showed good linearity, which is a typical characteristic of conduction heat transfer through a thin liquid layer. In result, the CLSM-LIF technique proposed in the present study was found to be a promising method to measure the local temperatures in the liquid flow field in microfluidic devices.

• Korean Journal of Optics and Photonics
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• v.9 no.2
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• pp.96-99
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• 1998

In this study, a novel long term stabilization method of optical feedback for the resonant cavity coupled semiconductor lasers is proposed, and its utility was shown experimentally. The proposed method is realized by using the pahse discriminator of optical feedback with high gain. The phase discriminating signal was obtained by the polarization spectroscopic technique using reflection light from the external reflector, which is a confocal Fabry-Perot cavity. Experimental result shows that stable control state can be maintained up to 20 hours. The period can be increased by reducing size of the system and/or fixing position stably of optical parts used, which were arranged on an optical table by using magnetic bases in this experiment. The proposed long-term stabilization method of optical feedack of a resonant external cavity coupled semiconductor laser is very useful for the field of high sensitivity measurement, and for the use in the laboratory level in particular.

• Applied Microscopy
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• v.46 no.1
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• pp.6-13
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• 2016

Fluorescence lifetime imaging microscopy (FLIM) has been considered an effective technique to investigate chemical properties of the specimens, especially of biological samples. Despite of this advantageous trait, researchers in this field have had difficulties applying FLIM to their systems because acquiring an image using FLIM consumes too much time. Although analog mean-delay (AMD) method was introduced to enhance the imaging speed of commonly used FLIM based on time-correlated single photon counting (TCSPC), a real-time image reconstruction using AMD method has not been implemented due to its data processing obstacles. In this paper, we introduce a real-time image restoration of AMD-FLIM through fast parallel data processing by using Threading Building Blocks (TBB; Intel) and octa-core processor (i7-5960x; Intel). Frame rate of 3.8 frames per second was achieved in $1,024{\times}1,024$ resolution with over 4 million lifetime determinations per second and measurement error within 10%. This image acquisition speed is 184 times faster than that of single-channel TCSPC and 9.2 times faster than that of 8-channel TCSPC (state-of-art photon counting rate of 80 million counts per second) with the same lifetime accuracy of 10% and the same pixel resolution.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.17 no.4 s.107
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• pp.341-350
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• 2006

In this paper, a new method for detecting breast cancer is proposed, which utilizes dielectric characteristics of pathological tissues and time delay of back scattered response, and its feasibility was investigated. We have developed a detection algorithm and verified it by numerical simulation and measurement for a prototype system. For a prototype system, we have fabricated experimental model(artificial breast with a cancer) and UWB(ultra-wideband) antenna. The results of the measurement simulation show an excellent detection capability of a cancer tissue. It is found that a good UWB antenna and a good calibration signal are key elements of such detection system. Further study is ongoing to develop a commercial system.