• Proceedings of the Korean Society of Precision Engineering Conference
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• 2004.10a
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• pp.426-429
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• 2004

To reconstruct the 3-D information of a irregular object, this paper proposes a new method applying the composition focus measure to time-series image. A focus measure function is carefully selected because a focus measure is apt to be affected by the working environment and the characteristics of an object. The proposed focus measure function combines the variance measure which is robust to noise and the Laplacian measure which, regardless of an object shape, has a good performance in calculating the focus measure. And the time-series image, which considers the object shape, is proposed in order to efficiently applying the interesting window. This method, first, divides the image frame by the window. Second, the composition focus measure function be applied to the windows, and the time-series image is constructed. Finally, the 3-D information of an object is reconstructed from the time-series images considering the object shape. The experimental results have shown that the proposed method is suitable algorithm to 3-D reconstruction of an irregular object.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.2
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• pp.283-294
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• 2007

Conventional culture-based methods of enumerating rumen microorganisms (bacteria, archaea, protozoa, and fungi) are being rapidly replaced by nucleic acid-based techniques which can be used to characterise complex microbial communities without incubation. The foundation of these techniques is 16S/18S rDNA sequence analysis which has provided a phylogenetically based classification scheme for enumeration and identification of microbial community members. While these analyses are very informative for determining the composition of the microbial community and monitoring changes in population size, they can only infer function based on these observations. The next step in functional analysis of the ecosystem is to measure how specific and, or, predominant members of the ecosystem are operating and interacting with other groups. It is also apparent that techniques which optimise the analysis of complex microbial communities rather than the detection of single organisms will need to address the issues of high throughput analysis using many primers/probes in a single sample. Nearly all the molecular ecological techniques are dependant upon the efficient extraction of high quality DNA/RNA representing the diversity of ruminal microbial communities. Recent reviews and technical manuals written on the subject of molecular microbial ecology of animals provide a broad perspective of the variety of techniques available and their potential application in the field of animal science which is beyond the scope of this treatise. This paper will focus on nucleic acid based molecular methods which have recently been developed for studying major functional groups (cellulolytic bacteria, protozoa, fungi and methanogens) of microorganisms that are important in nutritional studies, as well as, novel methods for studying microbial diversity and function from a genomics perspective.