• Journal of Microbiology and Biotechnology
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• v.12 no.2
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• pp.286-291
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• 2002

Histone deacetylase I (HDAC1) works as one of the components in a nucleosome remodeling (NuRD) complex that consists of several proteins, including metastasis-associated protein 1 (MTA1). Since the protein-protein interaction of HDAC1 and MTA1 would appear to be important for both the integrity and functionality of the HDAC1 complex, the interruption of the HDAC1 and MTA1 interaction may be an efficient way to regulate the biological function of the HDAC1 complex. Based on this idea, a yeast two-hybrid system was constructed with HDAC1 and MTA1 expressing vectors in the DNA binding and activation domains, respectively. To verify the efficiency of the assay system, 3,500 microbial metabolite libraries were tested using the paper disc method, and KB0699 was found to inhibit the HDAC1 and MTA1 interaction without any toxicity to the wild-type yeast. Furthermore, KB0699 blocked the interaction of HDAC1 and MTA1 in an in vitro GST pull down assay and induced morphological changes in B16/BL6 melanoma cells, indicating the interruption of the HDAC1 complex function. Accordingly, these results demonstrated that the yeast assay strain developed in this study could be a valuable tool for the isolation of a HDAC1 complex disruptor.

• Journal of Korean Society for Atmospheric Environment
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• v.14 no.2
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• pp.81-94
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• 1998

Six complex terrain dispersion models recommended by the U. S. Environmental Protection Agency were investigated using a hypothetical case in which a plume approaches complex terrain. The six models considered were Valley, CTSCREEN, COMPLEX 1, SHORTZ, RTDM, and CTDMPLUS, the latter four being closely studied. Highest concentrations were predicted for 48 receptors and plume behaviors were compared for stable and unstable meteorological conditions. Under stable conditions, ground-level concentrations were determined by the height of the plume centerline above the terrain. The concentrations estimated by SHORTZ and COMPLEX I were higher than those estimated by CTSCREEN, with CTDMPLUS predicting the lowest concentrations. In particular, the height of the lift midpoint, as well as the co.nterline of the plume, are important in the model calculation of CTDMPLUS. Under unstable conditions, the vertical dispersion plays a key role in determining ground -level concentrations. For this case, concentrations predicted by CTDMPLUS were the 'highest, whereas those predicted by SHORTZ were the lowest. Concentration distributions predicted by CTDMPLUS are quite similar to typical Gaussian distributions even on complex terrain, except for a slight shift of the plume centerline due to the of(tract of the geostrophic wind. In addition,24-hour average concentrations were estimated for comparison with results from the Valley model. Among the four models studied closely, CTDMPLUS predicted the lowest 24-hour average concentrations, but the concentrations estimated by Valley were lower than those estimated by CTDMPLUS.

• Journal of the Korean Ceramic Society
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• v.35 no.8
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• pp.783-790
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• 1998

The characterization of the complexation reaction of PVA and Al(III) ion at different pH and the sint-ering behaviour of UO2 containing the PVA-Al(III) complexes were investigated. Compared with pure PVA powder the complexed PVA-Al(III) powder had compacter shape and lower decomposition temperature The major phase of PVA-Al(III) complex decomposed at 90$0^{\circ}C$ was $\alpha$-Al2O3 The PVA-Al(III) complex formed at pH 9 had the lowest relative viscosity the highest Al content of 36% and the smallest particle size of 19${\mu}{\textrm}{m}$ While the pure UO2 pellet appeared with bimodal one. The grain size of the pure UO2 pellet was 7${\mu}{\textrm}{m}$ but that of the PVA-Al(III) complex added UO2 pellet was increased up to 36${\mu}{\textrm}{m}$ The largest grain size was ob-tained when the PVA-Al(III) complex formed at pH9 was added and the PVA-Al(III) complex formed at pH 11 had the greatest effect on increasing pore size.

• Molecules and Cells
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• v.19 no.1
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• pp.54-59
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• 2005

Deoxyribonuclease I (DNase I) is a divalent cation dependent endonuclease and thought to be a significant barrier to effective gene delivery. The only known DNase I-specific inhibitor is monomeric actin which acts by forming a 1:1 complex with DNase I. Its use, however, is restricted because of tendency to polymerize under certain conditions. We screened two random phage peptide libraries of complexity $10^8$ and $10^9$ for DNase I binders as candidates for DNase I inhibitors. A number of DNase I-binding peptide sequences were identified. When these peptides were expressed as fusion proteins with Escherichia coli maltose binding protein, they inhibited the actin-DNase I interaction ($IC_{50}=0.1-0.7{\mu}M$) and DNA degradation by DNase I ($IC_{50}=0.8-8{\mu}M$). Plasmid protection activity in the presence of DNase I was also observed with the fusion proteins. These peptides have the potential to be a useful adjuvant for gene therapy using naked DNA.

• Bulletin of the Korean Chemical Society
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• v.27 no.5
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• pp.687-693
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• 2006

Chromium(III) complexes, cis-[Cr([14]-decane)$(HOC _6H _4COO) _2$]$ClO _4$ I and cis-[Cr([14]-decane)(OH) $(OC _6H _4NO _2)$]$ClO _4{\cdot}H _2O$ II ([14]-decane = rac-5,5,7,12,12,14-hexamethyl-1,4,8,11-teraazacyclotetradecane) are synthesized and structurally characterized by a combination of elemental analysis, conductivity, IR and VIS spectroscopy, and X-ray crystallography. The complexes crystallizes in the monoclinic space groups, $C2 _1$/a in I and $P2 _1$/n in II. Analysis of the crystal structure of complex I reveals that central chromium(III) ion has a distorted octahedral coordination environment and two hydrogensalicylato ligands are unidentate to the chromium(III) ion via the carboxyl groups in the cis-position. For monomeric complex I the hydrogensalicylato coordination geometry is as follows: Cr-O(average) = 1.984(3) $\AA$;Cr-N range = 2.105(3)-2.141(4) $\AA$;C(24)-O(4) = 1.286(5) $\AA$;N(2)-Cr-N(4) (equatorial position) = 96.97(15)${^{\circ}}$; N(1)-Cr-N(3) (axial position) = 168.27(15)${^{\circ}}$; O(1)-Cr-O(4) = 85.70(13)${^{\circ}}$. The crystal structure of II has indicated that chromium(III) ion is six-coordinated by four secondary amines of the macrocycle, hydroxide anion and nitrophenolate anion.

• The Korean Journal of Zoology
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• v.36 no.2
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• pp.285-292
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• 1993

This investigation has been carried out to clarify structural architecture of cerebral neuroendocrine systems in the fifth instar lanra of cabbage butterfly Pieris rapae. In order to examine the cerebral neurosecretorv cell systems the brain and retrocerebral neuroendocrine complex were histochemically stained with the paraldehvde fuchsin. The brain of the fifth instar laMa contains three kinds of neurosecretorv cells: medial, lateral and tritocerebral neurosecretorv cells. The axon bundles of medial and lateral neurosecretory cells form medial neurosecretory pathway(MNSP) and lateral neurosecretorv pathwav(LNSP) within the brain respectively. Especially, prior to exiting the brain, the axon bundles of medial neurosecretorH cells located in both left and right cefebral hemispheres decussate in cerebral medial region and project to contralateral retrocerebral neuroendocrine complexes. Outside the brain the axon bundles of medial and lateral neurosecretory cells form the nenri corporis cardiaca(NCC) I and II respectively. The NCC I and ll run together to the retrocerebral neuroendocrine complex, forming the large nenre bundles in both left md right sides. The anon bundles of tritocerebral neurosecretory cells which pass through the brain along the tritocerebral neurosecretory pathway (TNSP) form the Ncc III outids the train. some of the Ncc I and it terminate in the corpus cardiacum, while the others pass through the corpus cardiacum without termination. The nerve bundle which passes the corpus cardiacum forms the nenrus corforis allatum(NCA) I which runs between the corpus cardiacum and the corpus allatum. Theyt are finally innervated to the corpus allatum. The Ncc III Projects to the corpus cardiRcum. However, most of NCC III priss through the corpus cardiacum without branching and then run down for another organ.

• Korean Journal of Crystallography
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• v.9 no.1
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• pp.1-5
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• 1998

The complex[Cu(L)I2, 2H2O(1) (L:3,14-Dimethyl-2,6,13,17-tetraazatricyclo [14,4,01.18, 07.12]docosane) has been prepared and structurally characterized. The complex (1) crystallizes in the triclinic system, space group P1, a=8.400(1)Å, b=8.986(3) Å, c=9.156(1) Å, α=82.42(1)˚, β=73.61(1)˚ λ=81.04(2)˚, Z=1, R for 1926 observed reflections of [F0>40(F0)], measured at 288K, was 0.042. The tetracoordination around Cu atom of complex(1) is square plane and the average bond distance of Cu-N is 2.029 (11) Å. Both six-membered chelate rings adopts a chair conformation and the five-membered ring are gauche with the sec-NH centers having the RRSS configuration.

• Cross-Cultural Studies
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• v.27
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• pp.167-195
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• 2012

A Chinese female writer Linhaiyin(林海音) and Chengnanjiushi(城南舊事), her representative work, have never been studied in Korea before although she and her literary works stand high and are well known in the history of the modern Chinese literature. Thus, in this paper, I analyze her and her novels included in Chengnanjiushi and study their literary value and meaning. To analyze and study them, I mainly consider novels Anhuiguan, Womenkanhaiqu, Lanyiniang, $L{\acute{\ddot{u}}}dagunr$, and Babadehuarluole included in Chengnanjiushi. Firstly, I look into her attitude and mode to observe children and women's life as a writer. Such attitude and mode succeeded to the spirit of the '5.4 literature'. Secondly, I evaluate Linhaiyin's 'Both sides complex' and its value in the history of literature. From this evaluation, the meaning of Linhaiyin's literature is highlighted. She was free from 'Both sides complex' occurred due to the circumstance of the times and played a role of a bridge so as not to break off literature of China and Taiwan. When her and her works are evaluated, this is one of the most important values. The characteristic of her writing mode is that she did not seek a compelling climax, a surprising reversal, or an exclusive plot or character in her works. In her works, plain description or unwitting conversation and story often imply deep meanings. Thus, at unexpected moment after reading her novels, readers truly listen to deep resonance for her attitude and mode to observe people's life. This is exactly her potential energy that makes readers sink into her literary world regardless of time and space.

• Animal Bioscience
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• v.37 no.6
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• pp.993-1000
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• 2024

Objective: This study was conducted to investigate the differential expression of the major histocompatibility complex (MHC) gene region in Eimeria-infected broiler. Methods: We profiled gene expression of Eimeria-infected and uninfected ceca of broilers sampled at 4, 7, and 21 days post-infection (dpi) using RNA sequencing. Differentially expressed genes (DEGs) between two sample groups were identified at each time point. DEGs located on chicken chromosome 16 were used for further analysis. Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis was conducted for the functional annotation of DEGs. Results: Fourteen significant (false discovery rate <0.1) DEGs were identified at 4 and 7 dpi and categorized into three groups: MHC-Y class I genes, MHC-B region genes, and non-MHC genes. In Eimeria-infected broilers, MHC-Y class I genes were upregulated at 4 dpi but downregulated at 7 dpi. This result implies that MHC-Y class I genes initially activated an immune response, which was then suppressed by Eimeria. Of the MHC-B region genes, the DMB1 gene was upregulated, and TAP-related genes significantly implemented antigen processing for MHC class I at 4 dpi, which was supported by KEGG pathway analysis. Conclusion: This study is the first to investigate MHC gene responses to coccidia infection in chickens using RNA sequencing. MHC-B and MHC-Y genes showed their immune responses in reaction to Eimeria infection. These findings are valuable for understanding chicken MHC gene function.

• Korean Journal of Crystallography
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• v.11 no.1
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• pp.28-33
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• 2000

The slow of 2,3-bis(2-pyridyl)pyarzine(bpp) with Ag(CF₃CO₂) produces crystalline product suitable for X-ray crystallography. Crystallographic characterization of the crystal (C/sub 32/H/sub 20/-F/sub 6/N/sub 8/O₄Ag₂: triclinic P1, a=8.518(5)Å, b=9.546(2)Å, c=10.632(1)Å, α=81.11(1)°, β=87.61(3)°, γ=75.66(3)°, V=827.5(5)ų, Z=1, R=0.0431) has provided that the complex is a cyclic dimer [Ag(bpp)(CF₃CO₂)]₂. Each bpp ligand connects two tetrahedral silver(I) ions in a tridentate mode (Ag-N, 2.26()-2.43(2)Å), and the trifluoroacetato anion is bonded to each silver(I) atom in a monodentate fashion (Ag-O, 2.38(1); 2.39(2) Å). The skeletal cyclic dimer is stable up to 212℃, and drastically decomposes around this temperature.