• Journal of Microbiology and Biotechnology
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• 제32권11호
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• pp.1416-1426
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• 2022

The need to discover new types of antimicrobial agents has grown since the emergence of antibiotic-resistant pathogens that threaten human health. The world's oceans, comprising complex niches of biodiversity, are a promising environment from which to extract new antibiotics-like compounds. In this study, we newly isolated Pseudomonas sp. NIBR-H-19 from the gut of the sea roach Ligia exotica and present both phenotypes and genomic information consisting of 6,184,379 bp in a single chromosome possessing a total of 5,644 protein-coding genes. Genomic analysis of the isolated species revealed that numerous genes involved in antimicrobial secondary metabolites are predicted throughout the whole genome. Moreover, our analysis showed that among twenty-five pathogenic bacteria, the growth of three pathogens, including Staphylococcus aureus, Streptococcus hominis and Rhodococcus equi, was significantly inhibited by the culture of Pseudomonas sp. NIBR-H-19. The characterization of marine microorganisms with biochemical assays and genomics tools will help uncover the biosynthesis and action mechanism of antimicrobial metabolites for development as antagonistic probiotics against fish pathogens in an aquatic culture system.

• 한국지하수토양환경학회지:지하수토양환경
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• 제15권1호
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• pp.50-56
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• 2010

Oil contamination soil has been one of the most environmental social issues for decades in the inside and outside of country. The law of soil environmental preservation was carried out in the 1990s and the government controlled not only soil environment management and the remediation of contaminated soil but also promoted the development of remedial technology and cleanup business of contaminated soil by national policy. In addition to agriculture areas, the main oil contaminated sites are a gas station, oil reservoir, petro-chemical complex, site of railway carriage base and military camp. The contamination-frequency of agriculture area and effect sites are low but it has significantly important area on account of producing food for human beings. Therefore, we should be concerned about oil contamination damage of agriculture area. The oil contamination damage of agriculture area influenced drop of birth and breeding since the oil directly adheres to seeds and farm products even diffusion of contaminated soil to cultivation area. The studies of the crops and the food vegetation has not enough detailed data caused by the incident of oil contamination. This study investigated the effect of oil in germination and growth of selected plant seeds. In this study, we try to verify whether the oil contamination by accidents on farmland influenced the damage of farm produce and the mutual relation both oil contaminated soil or the vegetation of crops. The impact of oil on plant development was followed by phytotoxicity assessments. The plants exhibited visual symptoms of stress, growth reduction and perturbations in developmental parameters. The increase of the degree of pollution induced more marked effects in plants, likely because of the physical effects of oil. The relationships between the phytotoxicity contents of plants and growth reduction suggest a chemical toxicity of fuel oil. In addition, while cleaned up the contaminated soil under the standard of contaminated soil we examined it was suitable for region standard and it may have practical possibility for fill material of construction of afforestation and molding soil of landfill.

• 환경정책연구
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• 제14권2호
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• pp.27-47
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• 2015

투입 산출변수의 외생적 흐름에 기초한 전통적인 자료포락분석은 생산활동의 모든 내부과정을 'black box'로 간주하며 각 생산단계의 상호작용에 대한 정보를 고려하지 못한 한계를 지닌다. 이에 생산과정을 세분화하고 각 생산단계의 효율성을 연계하여 추정하는 Network DEA 방식이 최근 활발히 적용되고 있으며 특히 환경효율성의 추정과정을 생산 및 저감활동으로 구분한 연구가 대표적이다. 본고는 한국의 51개 산업단지를 대상으로 Network DEA에 기초한 환경효율성 추정을 시도하였으며, 분석결과 추정치의 변별력, 비효율의 발생단계, 기타 외생변수와의 통계적 관련성 등의 관점에서 전통적 DEA 추정결과에 비해 우수한 점을 확인할 수 있었다.

• 한국산업보건학회지
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• 제33권3호
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• pp.355-364
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• 2023

Objectives: The objective of the present study was to identify mycobacterial infection in retired dusty workers who were ineligible for medical care benefits for work-related pneumoconiosis. Methods: Sputum samples were collected from 170 retired dusty workers living in Gangwon-do. The mycobacterial culture was grown in 2% Ogawa medium and Mycobacteria Growth Indicator Tube(MGIT). Mycobacterial species were identified using MolecuTech REBA Myco-ID^Ⓡ. Results: Thirty-one(18.2%) out of 170 sputum samples were identified as positive for culture. Among the positive culture samples, eleven(6.5%) were identified as mycobacterial species. The proportion of mycobacteria was M. avium 2.3%(4/170), M. fortuitum complex 1.2%(2/170), M. intracellulare 1.2%(2/170), M. abscessus 0.6%(1/170), M. tuberculosis(MTB) complex 0.6%(1/170), and MYC(NTM except 19 species) 0.6%(1/170). Conclusions: In comparison with previous studies, the incidence rate of tuberculosis(TB) in retired dusty workers who were ineligible for medical care benefits for work-related pneumoconiosis was higher than in close contact with TB patients, workers exposed to silica, and patients with silicosis. And the proportion of non-tuberculosis mycobacteria(NTM) was higher than that of MTB.

• Asian-Australasian Journal of Animal Sciences
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• 제20권4호
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• pp.501-510
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• 2007

The present study was conducted to establish primary bovine muscle satellite cell (MSC) culture conditions and to investigate the effects of various steroid hormones on transcription of the genes involved in muscle cell proliferation and differentiation. Of three different types of proteases (type II collagenase, pronase and trypsin-EDTA) used to hydrolyze the myogenic satellite cells from muscle tissues, trypsin-EDTA treatment yielded the highest number of cells. The cells separated by hydrolysis with type II collagenase and incubated on gelatin-coated plates showed an enhanced cell attachment onto the culture plate and cell proliferation at an initial stage of cell growth. In this study, the bovine MSCs were maintained in vitro up to passage 16 without revealing any significant morphological change, and even to when the cells died at passage 21 with decreased or almost no cell growth or deformities. When the cells were incubated in a steroid-depleted environment (DMEM(-)/10% CDFBS (charcoal-dextran stripped FBS)), they grew slowly initially, and were widened and deformed. In addition, when the cells were transferred to an incubation medium containing steroid (DMEM(+)/10% FBS), the deformed cells resumed their growth and returned to a normal morphology, suggesting that steroid hormones are crucial in maintaining normal MSC morphology and growth. The results demonstrated that treatments with 19-nortestosterone and testosterone significantly increased AR gene expression (p<0.05), implying that both testosterone and 19-nortestosterone bind with AR and that the hormone bound-AR complex up-regulates the genes of its own receptor (AR) plus other genes involved in satellite cell growth and differentiation in bovine muscle.

• 한국가금학회지
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• 제38권4호
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• pp.275-284
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• 2011

As the world population grows and developing countries become more affluent, the global consumption of meat will increase by more than 50% within the next 10 years. Confronting the increased demand for poultry food products are emerging field diseases, increasing regulatory bans of antimicrobial growth promoters, high-density growth conditions, and waste management. Although biotechnology offers solutions to some of these challenges, basic studies are needed to better understand the complex interaction between the intestinal microbiome, host immunity and the environment. This presentation will focus on emerging strategies to enhance gut immunity and to decrease economic losses due to poultry diseases. This presentation will highlight recent developments in coccidiosis research and provide information on host immunity, immunomodulation, and the latest advances in dietary and nutritional approaches against coccidiosis. Such information will magnify our understanding of host-parasite biology, mucosal immunology, and design of future nutritional interventions and vaccination strategies for coccidiosis.

• 식품과학과 산업
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• 제50권3호
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• pp.33-38
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• 2017

One noticeable thing about the recently opened 'Starfield Hanam' and 'Hyundai Department Store (Pangyo)' is consumer's interest and positive response to their hosted food brands ranging from restaurants, dessert cafes and coffee shops. Department store and shopping complex, so-called aggregation of lifestyle are making the most out of food brands as a differentiation strategy and consumer's interest on it act as a barometer for current consumption trend. Along with the high interest on food market, changes in lifestyle, such as increasing ratio of two or less households, advent of multiple communication channels as SNS, deluge of information and gradually developing individualism, are creating needs for convenient but high quality eating culture. This need contributed in transforming the product family which was once famous as 'Instant Food' into a 'Home Meal Replacement (HMR)'. Since local food companies are striving to keep pace with the trend and actively penetrating HMR market, regardless of long-term recession, the Korean domestic food market is steadily growing with a bright future.

• 한국환경농학회지
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• 제41권3호
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• pp.177-184
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• 2022

BACKGROUND: Recently, the inflow of nonpoint pollutants into rivers caused by rapid urban and industrialization promotes the proliferation of algae, which causes eutrophication of rivers. This study was conducted to evaluate the seasonal variation of water quality characteristics and cryptomonads growth in the Oncheon River. METHODS AND RESULTS: The water quality and distribution characteristics of cryptomonads in the Oncheon River were investigated monthly for 12 months from January 2021. The cell number of cryptomonads was intensively developed in January-April, and it decreased sharply in the summer with heavy rainfall. In particular, cryptomonads moved to the downstream side of the river depending on the time, and as a result, significant differences were shown for each investigation point. The Korean trophic state index (Tsi_KO) in Oncheon River was classified as eutrophy all year round, indicating that cryptomonads can grow year-round. Distribution characteristics of cryptomonads in Oncheon River showed high correlations with DO (r=0.678), BOD (r=0.826) and chlorophyll-a (r=0.613) in water. CONCLUSION(S): In order to reduce cryptomonads in the Oncheon River, it is judged that a complex countermeasure considering the residence time, insolation and precipitation along with water quality factors is required.

• 생태와환경
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• 제46권1호
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• pp.135-144
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• 2013

본 연구는 청정수역으로 알려져 있던 북한강 수계에서 저수온기에 우점한 남조류 Anabaena spiroides 현존량과 이취미 물질인 지오스민 (geosmin)에 대해 조사하고 환경인자와의 통계적 분석을 통하여 A. spiroides의 증식 특성을 알아보고자 하였다. 2011년 11월 말에 A. spiroides가 과다증식하였는데 출현지역은 의암호 내의 공지천 유입부 부터 하류의 팔당호까지 그 범위가 넓고 하류로 확산되는 특성을 보였다. 저수온기에 수온 상승과 체류시간 증가 등의 물리학적 수환경 변화가 남조류 A. spiroides의 과다증식에 영향을 미쳤다. A. spiroides 현존량은 팔당호에서 $11,325cells\;mL^{-1}$로 최대증식을 보였으며, 수온이 $4^{\circ}C$ 이하로 내려가면서 A. spiroides 현존량이 급격히 감소하였다. 수환경 요인 중에서 수온 하강이 A. spiroides 현존량 감소에 가장 큰 영향을 준 인자였다. 남조류의 대사물질로 이취미의 원인물질인 Geosmin은 팔당호에서 최대 $1,640ng\;L^{-1}$ 농도를 나타냈다. 북한강 수계에서 Geosmin 농도는 A. spiroides와 매우 유의한 상관관계를 보여 A. spiroides에 의해 이취미가 유발되었음을 시사하였다.