• Title/Summary/Keyword: Complex Growth Environment

Search Result 194, Processing Time 0.023 seconds

Quantitative Polymerase Chain Reaction for Microbial Growth Kinetics of Mixed Culture System

  • Cotto, Ada;Looper, Jessica K.;Mota, Linda C.;Son, Ahjeong
    • Journal of Microbiology and Biotechnology
    • /
    • v.25 no.11
    • /
    • pp.1928-1935
    • /
    • 2015
  • Microbial growth kinetics is often used to optimize environmental processes owing to its relation to the breakdown of substrate (contaminants). However, the quantification of bacterial populations in the environment is difficult owing to the challenges of monitoring a specific bacterial population within a diverse microbial community. Conventional methods are unable to detect and quantify the growth of individual strains separately in the mixed culture reactor. This work describes a novel quantitative PCR (qPCR)-based genomic approach to quantify each species in mixed culture and interpret its growth kinetics in the mixed system. Batch experiments were performed for both single and dual cultures of Pseudomonas putida and Escherichia coli K12 to obtain Monod kinetic parameters (μmax and Ks). The growth curves and kinetics obtained by conventional methods (i.e., dry weight measurement and absorbance reading) were compared with that obtained by qPCR assay. We anticipate that the adoption of this qPCR-based genomic assay can contribute significantly to traditional microbial kinetics, modeling practice, and the operation of bioreactors, where handling of complex mixed cultures is required.

Experimental Study on Heat Exchange Efficiency of Combined Well & Open-Closed Loops Geothermal System (지하수정호와 결합한 복합지열시스템의 열교환 효율에 대한 실험적 연구)

  • Song, Jae-Yong;Lee, Geun-Chun;Park, Namseo
    • Journal of the Architectural Institute of Korea Structure & Construction
    • /
    • v.34 no.5
    • /
    • pp.43-50
    • /
    • 2018
  • The temperature of underground water generally remains constant regardless of the season. therefore, it is possible to get plenty of energy if we use characteristics of underground water for both cooling and heating. This study evaluates efficiency of real size coaxial and U-tube type complex geothermal system which is combined with underground water well. This study also evaluates relative efficiency/adaptability through comparison with existing geothermal systems(vertical closed loop system, open loop system(SCW)). The heat exchange capacity of complex geothermal system according to temperature difference between circulating water and underground water shows very high significance by increasing proportionally. The temperature change of underground water according to injection energy, shows very high linear growth aspect as injection thermal volume heightens. As a result of evaluation of heat exchange volume between complex geothermal system and comparative geothermal system, coaxial type has 26.1 times greater efficiency than comparative vertical closed type and 2.8 times greater efficiency than SCW type. U-tube type has 26.5 tims greater efficiency than comparative vertical closed type and 2.8 times greater than SCW type as well. This means complex geothermal system has extremely outstanding performance.

Air Quality Assessment for Development Plan after the Special Act on Licensing of Industrial Complexes (산업단지 「인·허가 특례법」 이후 개발계획에 따른 국내 대기질 영향예측)

  • Moon, Nankyoung;Kim, Soontae;Seo, Jihyun
    • Journal of Environmental Science International
    • /
    • v.26 no.11
    • /
    • pp.1209-1222
    • /
    • 2017
  • On June 5, 2008, the "Act on Special Cases Concerning the Simplification of Authorization and Permission Procedures for Industrial Complexes" (Act No. 9106) was enacted. When it was implemented in August 2008, many industrial complex development projects were established, and the number of industrial complexes growth rates of 3-6% during 2003-2007 rose to around 15% in 2008. With the increase in industrial complexes, the environmental impacts of individual projects were examined, but comprehensive regional reviews of environmental impacts were not undertaken. In this study, we determined changes in air quality by applying the industrial complex development plan that completed the consultation at the end of 2010 to assess the comprehensive regional environmental impacts and presented the adequacy review plan for future industrial development plans based on the study's results. When considering these industrial complex development plans, emissions in North Jeolla and South Chungcheong Provinces and Daegu City have increased significantly. Air quality analyses showed that the 24 h mean $SO_2$ concentration in Daegu increased by more than 50% in summer compared to air quality concentrations in summer. The 24 h mean $PM_{10}$ and $NO_2$ concentrations increased by approximately 12 and 30%, respectively, in North Jeolla Province in summer. Areas exceeding the air quality standard for 1 h mean $O_3$ concentration increased by more than $3,500km^2$. Based on the above analysis, changes in air quality should be anticipated through a comprehensive evaluation of long-term development plans. Furthermore, control of air quality in accordance with the development of future industrial complexes is possible.

Sensitivity to Sterol Biosynthesis Inhibitors of Colletotrichum gloeosporioides Isolated from Persimmon in 2013 in Sangju, Gyeongsangbukdo (2013년 경북 상주지역 감나무로부터 분리한 Colletotrichum gloeosporioides 탄저병균의 스테롤 생합성 저해 살균제에 대한 감수성)

  • Lim, Tae Heon;Lee, Dong Woon;Kwon, Oh Gyeong;Han, Sangsub;Cha, Byeongjin;Song, In Kyu
    • The Korean Journal of Pesticide Science
    • /
    • v.19 no.3
    • /
    • pp.272-278
    • /
    • 2015
  • Colletotrichum gloeosporioides is one of the most serious pathogens of persimmon in Korea. In 2013, 67 isolates of C. gloeosporioides were isolated from infected fruits, leaf and twigs of persimmon (Diosprosi kaki) at Sangju, Gyeongsangbukdo and fungal responses against five fungicides (prochloraz manganese complex, tebuconazole, mancozeb+myclobutanil, fluquinconazole+prochloraz, and tebuconazole+tolyfluanid) were evaluated by their growth on the fungicide-medium. All isolates were inhibited mycelium growth on the medium with each recommended application concentration of flied. $EC_{50}$ (${\mu}g/ml$) of tebuconazole was from 0.02 to 1.04 and average was 0.31. $EC_{50}$ (${\mu}g/ml$) of prochloraz manganese complex was 0.02~0.23 average was 0.078. Average $EC_{50}$ values (${\mu}g/ml$) of Fluquinconazole+Prochloraz (FP) was 0.029. On the basis $EC_{50}$ (${\mu}g/ml$), the correlation coefficient (r) between tebuconazole and prochloraz manganese complex, prochloraz manganese complex and FP, tebuconazole and FP were 0.42, 0.44 and 0.27, respectively.

A Study on the Change in Forest Community by Air Pollution at Yocheon District (大氣汚染에 依한 麗川地域의 森林群集變化에 關한 硏究)

  • Kim, Joon-Seon;Kim, Tae-Wook
    • Journal of Korean Society for Atmospheric Environment
    • /
    • v.2 no.3
    • /
    • pp.1-10
    • /
    • 1986
  • Nowadays, air pollution by increasing consumption of fossil fuels resulting from rapidly growing population and industrialization has caused the adverse effects on terrestrial ecosystems and become one of the most serious problems causing environmental discriptions. Air pollution such as $SO_2, HF, NO_X,$ fly ash, ozone and PAN might influence plant growth, reproduction, nutrient cycling, photosynthesis and predisposition to entomological and pathological stresses on plants. Furthermore, accumulation of those toxic substances in forests might cause subtle or serious changes in the structure and function of forest ecosystems. Since 1970s, a number of large industrial complexes had been constructed as a part of industrialization plan in Korea. Accordingly, the forest exosystems around them has been under chronic influences of air pollution and effects of air pollution on plants became a matter of concern. In Yocheon Industrial Complex which consisted of lots of petrochemical plants and a phosphatic fertilizer manufacturing plant, forests has been exposed to chronic air pollution, mainly HF and $SO_2$ gas, Various reports were available to investigate the potential effects of air pollution on crops and forest trees in Yocheon. Kim and Kim surveyed vegetation by naked eye method and reported 71 families, 150 genera and 158 species were growing within a 2 km from air pollution sources in 1981. Needle injuries on Pinus spp. in the polluted area water reported by Kim, et al. and Kim, et al. Kim, et al. investigated the primary production of Pinus thunbergii forests in the polluted area and verified that growth inhibition of Pinus thunbergii was attributable to air pollution. Thus, previous reports suggested that forest ecosystems around Yocheon Industrial Complex were influenced adversely by air pollution. The objective of this study was to investigate the subtle ecological changes in forest community exposed to chronic air pollution in Yocheon.

  • PDF

A Study on Anaerobic Biodegradation of MTBE and BTEX by Indigenous Microorganisms (토착 미생물을 이용한 MTBE와 BTEX의 혐기성 생분해 연구)

  • Chung, Woo-Jin;Chang, Soon-Woong
    • Journal of Soil and Groundwater Environment
    • /
    • v.21 no.3
    • /
    • pp.88-94
    • /
    • 2016
  • The simultaneous biodegradation between MTBE (Gasoline additives) and BTEX (Benzene, Toluene, Ethyl-benzene, o-Xylene, m-Xylene, p-Xylene) was achieved within a competitive inter-relationship, with not only electron accepters such as nitrate, sulfate, and iron(III) without oxygen, but also with electron donors such as MTBE and BTEX. Preexisting indigenous microorganisms from a domestic sample of gasoline contaminated soil was used for a lab-scale batch test. The result of the test showed that the biodegradation rate of MTBE decreased when there was co-existing MTBE and BTEX, compared to having just MTBE present. The growth of indigenous microorganisms was not affected in the case of the MTBE treatment, whereas the growth of the microorganisms was decreased in combined MTBE and BTEX sample. This may indicate that an inhibitor related to biodegradation when BTEX and MTBE are mixed will be found. This inhibitor may be found to retard the anaerobic conditions needed for efficient breakdown of these complex carbon chain molecules in-situ. Moreover, it is also possible that an unknown competitive reaction is being imposed on the interactions between MTBE and BTEX dependent on conditions, ratios of mixture, etc.

Estimation of Development Valuation at Marine Tourism complex (해양레저관광단지의 개발가치 추정)

  • Jang, Woon-Jae;Keum, Jong-Soo
    • Proceedings of KOSOMES biannual meeting
    • /
    • 2008.05a
    • /
    • pp.69-74
    • /
    • 2008
  • The aim of this paper is to calculation benefit cost for development of Hwawon leisure & tourism complex. To calculation benefit cost, this paper propose a advanced travel cost method(ATCM) which is to consider Improved travel cost method(ITCM) and variable traffic units with confidence degree cf decision-maker. At the result of calculation, total benefit costs are 292805 million won at 52% of confidence degrees and 10% discount rates.. Also total benefit cost is 304517 million won at 4% of GDP growth rate is 4%.

  • PDF

A Study on the Minimum Distance between Landscape Trees and Apartment Buildings (수목생육 조건을 고려한 조경수목과 아파트 건물의 최소 이격거리에 관한 연구)

  • Jang, Ha-Kyung;Ahn, Geun-Young;Lee, Eun-Heui
    • Journal of the Korean Institute of Landscape Architecture
    • /
    • v.35 no.1 s.120
    • /
    • pp.1-8
    • /
    • 2007
  • The purpose of this study is to suggest the minimum distance needed between landscape trees and apartment buildings. in order for the trees to grow soundly and maintain constant growth. Therefore, this study investigated and analyzed the present conditions of the green-spaces contiguous to apartment buildings and the trees inside the apartment complex. The following general problems were identified : (1) the tree growth conditions, (2) the planting intervals, and (3) the planting methods in the apartment complexes. The method of this study was to survey areas, measure the trees and analyze the results. Ten apartment complexes located in Seoul were randomly selected for this study. To analyze the greens contiguous to the building of each apartment complex, the greens were divided into three types, including the front greens, the side greens, and the rear greens. The study surveyed the width of all contiguous greens and the distance from trees to my given building. Four representative sites were specifically investigated to measure the crown widths, heights, and diameters of the trees. These investigations were carried out over 3 months from August to December 2006. According to the results of the study, it was found that the greens are narrower in width and the more closer to a building. The study identified a correlation between the tree and the passage of time after the completion of construction of an apartment complex, showing that the malformation of the trees worsened as time passed. As a result of measuring the right crown and left crown width with the tree trunk as the center, a broad difference was found between coniform trees and trees that tend to branch out more. For example, the ratio of the width of the Acer palmatum is 1:6. However, the ratio of the Metasequoia glyptostroboide is 1:1.7. Based on the overall analysis, it was concluded that the maintained minimum width must be greater than one meter over the green space according to the 'Landscape Standard 2000'. However, that is only applicable to cases such as apartment buildings that present no obstacles. In conclusion having greens placed within one meter of width to a building is not an appropriate environment for the healthy growth of trees. To create sound green spaces, sustainable, long-term growth rate and size should be considered. It is necessary to prepare new standards for the computation of planting trees and the measurement of square landscapes. Wall greenery and shrubberies could be an alternative in the case of a narrow-width green space.

A Design of Greenhouse Control Algorithm with the Multiple-Phase Processing Scheme (다중 위상 처리구조를 갖는 온실 복합환경제어 알고리즘 설계)

  • Daewook Bang
    • Journal of Service Research and Studies
    • /
    • v.11 no.2
    • /
    • pp.118-130
    • /
    • 2021
  • This study designs and validates a greenhouse complex environmental control algorithm with a multi-phase processing scheme that can combine and control actuators according to the degree of change in the greenhouse environment. The composite environmental control system is a system in which the complex environmental controller analyzes the information detected by sensors and operates appropriately actuators to maintain the crop growth environment. A composite environmental controller directs control devices driving actuators through a composite environmental control algorithm, which calculates the values necessary for the operation of the control devices. Most existing algorithms carry out control procedures on a single phase by iteration cycle, which can cause abnormal changes in the greenhouse environment due to errors in output. The proposed algorithm distributes control procedures over multiple phases: environmental control, environmental control, and device operation, and every iteration cycle, detects environmental changes in the environmental control phase first, and then combines control devices that can control the environment in the environmental control phase, and finally, performs the controls to derive the actuators in the device operation phase. The proposed algorithm is designed based on the analysis of the relationship between greenhouse environmental elements and control devices deriving actuators. According to verification analysis, the multi-phase processing scheme provides room to modify or supplement the setting value and enables the control devices to reflect changes in the associated environmental components.

Portfolio of Real Estate Price Index for ICT Environment Study on Diversification Effect (ICT 환경에서 부동산 가격지수 포트폴리오 분산효과에 관한 연구)

  • Jang, Dae-Seub;Min, Guy-Sik
    • The Journal of the Korea institute of electronic communication sciences
    • /
    • v.9 no.3
    • /
    • pp.393-402
    • /
    • 2014
  • ICT environment to the survey released by the Bureau of Statistics 2012 Household Finance. Korean Welfare survey 24.9% of all households in financial assets, real estate is about three times more than 69.9%, respectively. The problem is that the information is slow and income deciles(deciles 1-4), a relatively high proportion of households with low(78.8 to 69%) of the real estate assets of the expansion of the world economy with low growth and low uncertainty, work from home due to the information changes in the structure of the economy, such as increases in real estate prices remain exposed to the risk of a phenomenon such as Pour House Pour Talent and low-income people is bound to be more serious symptoms. This low correlation is by constructing a composite asset portfolio, the weighted average risk of the individual assets while increasing overall revenue decrease that risk is based on the principle of portfolio by type and different areas in the ICT environment in a portfolio of real estate price index low correlation to financial assets by including the effect of dispersion stable complex asset portfolio and empirical Growth was divided.