• Archives of Plastic Surgery
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• 제35권4호
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• pp.400-406
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• 2008

Purpose: Since ancient times, larval therapy has been applied to help wound healing. Its use has recently been rediscovered, and the interest in this therapy has been increased in clinical practice, as well as in research. The objective of this study is to assess the efficacy of maggot therapy for the treatment of intractable, chronic wounds. Methods: Twenty patients, suffering mostly from chronic wounds were treated using maggots of greenfly (Phaenicia sericata). Sterile maggots were administered to the wound twice a week. The causes of the development of wounds were diabetic foot(14), trauma(3), plate exposure(1), and unknown origin(2). Results: Complete debridement was achieved in 10 wounds; in 8 wounds, the debridement was partially achieved; 2 patients escaped. Four patients were healed without any additional surgical procedures but fourteen patients were treated with skin graft. Pain, reported by six patients, was relatively well controlled with oral analgesics. Conclusion: We found larval therapy to be effective for chronic wounds. Maggot therapy is a relatively rapid and effective treatment, particularly in necrotic wounds or the wounds resistant to conventional treatment.

• Fisheries and Aquatic Sciences
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• 제21권9호
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• pp.23.1-23.8
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• 2018

Background: Russian sturgeon (Acipenser gueldenstaedtii) is an emerging candidate species in the Korean aquaculture domain owing to its highly valued caviar. Although the embryonic development of this species was previously described, the complete image data on the morphological differentiation of developing embryos have not been yet fully available. Further, with the viewpoint of larval production in hatchery, the effects of temperature on embryonic viability and the temporal window of hatching event have not been extensively studied. Hence, the objective of this study was to provide a complete set of photographic image data on the embryogenesis and also to examine the effects of incubation temperatures on embryonic viability and hatching event in farm-bred Russian sturgeon. Results: Typical characteristics of embryonic development including uneven, holoblastic cleavages with unequal blastomeres, followed by the formation of germ layer, neurulation, and organogenesis until hatching, were documented. Under different temperature conditions (12, 16, or $20^{\circ}C$), viability of embryos incubated at $12^{\circ}C$ was significantly lower as relative to those of 16 and $20^{\circ}C$ incubated embryos. Hatchability of embryos was higher, and the timing of hatching event was more synchronized at $20^{\circ}C$ than at 12 and $16^{\circ}C$. Conclusion: Data from this study suggest that the incubation of Russian sturgeon embryos at $20^{\circ}C$ would be desirable in the hatchery practice with respect to the good hatchability of embryos and the synchronization of hatching events. Additionally, the updated image data for complete embryonic development could be a useful reference guide for not only developmental researches but also artificial propagation of Russian sturgeon in farms.

• 한국해양학회지:바다
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• 제5권3호
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• pp.233-237
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• 2000

Harpacticoida 목, Diosaccidae 과에 속하는 Paramphiascella vararensis를 실험실내에서 배양하여 nauplius 단계의 유생 발생을 기술, 도시하였다. P. vararensis는 부화 후 VI단계의 nauplius 유생기가 관찰되었으며, 제 VI nauplius 단계 이후에는 copepodid로 탈피하였다. 22${\sim}$23$^{\circ}$C의 배양 온도, 33${\sim}$34ppt의 염분 농도 그리고 구멍 갈파래의 분말을 먹이로 급이하면서 사육한 결과, 부화 후 nauplius VI 단계까지 12일이 소요되었다. nauplius 유생의 형태는 납작하고 체장의 길이보다 체폭의 길이가 긴 타원형이며, 전방에 하나의 붉은색 안점을 가지고 있었으며, 크기의 범위는 35${\sim}$98${\mu}$m였다. 그리고 제 2촉각의 저절과 기절의 강모식에 의해 제 I nauplius 유생기에서 제 IV nauplius 유생기까지 발생 단계의 식별이 가능하며, 제 V nauplius 유생기와 제 VI nauplius 유생기는 제 2소악과 미부의 강모식에 의해 식별이 가능하였다. P. vararensis의 nauplius 유생기에서 제 1소악의 원기는 제 III nauplius 유생기에서 관찰되었으며,제 2소악의 원기는 제 V nauplius 유생기에서 관찰되었다.

• International Journal of Industrial Entomology and Biomaterials
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• 제14권2호
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• pp.127-135
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• 2007

Precocious metamorphosis was induced in two popular bivoltine breeds i.e. YB and $NB_4D_2$ by an imidazole compound having anti-juvenile hormone activity. The chemical was administered by feeding treatment with mulberry leaf to freshly moulted (0-6 hrs) IV instar larvae. The dose of the chemical was found to be breed specific being 650 ppm for YB and 500 ppm for $NB_4D_2$. The chemical caused complete skipping up of the fifth instar larvae which is most susceptible to diseases. But IV instar was somewhat prolonged. As a result the total larval period was reduced by 4-5 days. However, some of the rearing and reeling parameters like cocoon yield, cocoon weight, shell weight, shell %, filament length and fecundity were reduced for that particular generation only. The effective rate of rearing (ERR %) was significantly increased in trimoulters during the most unfavourable August - September seed crop season. Number of cocoons / kg and number of male moth were significantly higher in trimoulters. These may be useful for preparation of $multi{\times}bi$ hybrid seed. Fine denier was also found in trimoulter cocoon which is the cause of getting fine silk filament from trimoulter cocoons. Normal mating behaviour and emergence pattern was recorded in trimoulters. Bivoltine trimoulter males also showed competence for mating with multivoltine females. These results suggest the possibility of getting trimoulter males during hot and humid seasons when rearing of bivoltine is almost impossible particularly in Eastern and North Eastern India.

• 한국수산과학회지
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• 제8권4호
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• pp.225-233
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• 1975

1968년 6월,1969년 7월 및 1971년 7월에 해운대 동백섬 연안에서 채집된 953개체의 점망둑 자치어를 재료로 하여 초기생활사에 따른 형태변화 과정중 기조수의 변화, 색소포의 발달과정 및 배지느러미의 형성과정을 관찰하였다. 본 종은 제1등지내러미의 기조원기는 8.0 mm 전후에서 생겨나며 9.2mm 전후에서 완성하며 모든 지느러미의 연조는 초기치어기인 17.0mm 전후에서 완성된다고 생각된다. 색소포의 발달은 3단계로 구분할 수 있는데 후기자어기$(6.3\~14.2mm)$ 있어서 몸의 배면과 미부 복측면 및 꼬리지느러미 기저에 분포하고 있는 경우와, 후기 자어말기$(17.0\~2.44mm)$에 미부의 체측정중선에 일단의 색소총이 생기고 이것이 점점 전방으로 확대분포하는 시간 및 초기치어기$(27.2\~34.8mm)$에 몸 전체에 걸쳐 운형 및 H자상으로 분포되어 종의 특징을 나타내는 시기로 구분된다. 배지느러미의 형성과정은 후기자어기인 전장 7.4mm일 때 기조의 원기가 나타나서 17.0mm 전후에 기조가 완성되며 전장 30.0mm 전후에서 흡반이 완성된다.

• Molecules and Cells
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• 제23권3호
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• pp.379-390
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• 2007

We sequenced and characterized the complete mitochondrial genome of the Japanese fish tapeworm D. nihonkaiense. The genome is a circular-DNA molecule of 13607 bp (one nucleotide shorter than that of D. latum mtDNA) containing 12 protein-coding genes (lacking atp8), 22 tRNA genes and two rRNA genes. Gene order and genome content are identical to those of the other cestodes reported thus far, including its congener D. latum. The only exception is Hymenolepis diminuta in which the positions of trnS2 and trnL1 are switched. We tested a PCR-based molecular assay designed to rapidly and accurately differentiate between D. nihonkaiense and D. latum using species-specific primers based on a comparison of their mtDNA sequences. We found the PCR-based system to be very reliable and specific, and suggest that PCR-based identification methods using mtDNA sequences could contribute to the study of the epidemiology and larval ecology of Diphyllobothrium species.

• The Korean Journal of Ecology
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• 제14권4호
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• pp.449-455
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• 1991

The water striders, gerris paludum insularis larvae which were fed with the drosophila melanogaster cultured on the appli diet and artificial diet, were reared in the growth cabinet controlled as the two constant temperature regimes of 25 c and 30 c under the condition of photoperiod 16l : 8d, light intensity 510 240 lux, relative humidity 65 3%, and in the natural state. The effects of temperature and diet on the devolopment of g. paludum insularis were analyzed and the results were summarized as follows. 1. The total developmental period of the water strider larvae fed with the d. melanogaster reared on the apple diet at the natural stste was the longest 40 days, and the total devopmental periods at 25 c and 30 c were 35 days and 27 days respectively. The tolal developmental period of larvae fed with d. melanogaster reared on the artifical diet at 25 c was the longest 42 dats, and the total developmental periods at natural stste and 30 c were 32 days and 27 days, respectively. 2. In the larval developmental stages, the fifth stage was longer than that of any stage. The duration of egg stage which was not influenced by diet at 25 c was the longest. 3. The water strider larvae fed which the d. melanogaster reared on the artificial diet at 25 c did not complete their devolopment. 4. No significant difference was odserved in the developmentperiod for diet at 30 c. but, there were significant differences in developmental periods for diet at 25 c and natural state. 5. The rate of adult emergence on the apple diet was higher than that of the artificial diet was higher than of the artificial diet. 6. The shapes of the survivorship curve were convex-curve. 7. The development periods of the larval stages of water striders might be affected to temperature and diet dependently.

• 한국패류학회지
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• 제15권2호
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• pp.115-119
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• 1999

We investigated the processes of egg and larval developments for aquaculture technique development of seedling production fo the flat oyster, Ostrea denselamellosa. Teo flat oyster of larviparous type was different from the pacific oyser (ovivarous type) because their larvae (trochophore and prodissoconch larvae) in the gill released into the seawater. The process of egg development was observed by artificial fertilization at $25^{\circ}C$, using a dissecting method. The sizes of Unfertilized eggs ranged from 80 to 90 $\mu\textrm{m}$ and fertilized eggs with globule-shape was 90-100 $\mu\textrm{m}$. The Polar body appeared after fertilization and egg cleavage began within 1 hour, reaching the blastula stage after 10 hours. The trochophore in the gill appeared 2-3 days after fertilization and grew to the prodissoconch larvae (130 140 $\mu\textrm{m}$) having a complete shell after 1-2 days. The shell of prodissoconch larvae grew to 205 220 $\mu\textrm{m}$ after 10 hours, and then they became umbo stage larvae showing oval in shape. The velum of umbo stage larvae was degenerated about 17-20 days after fertilization and grew into a pediveliger with a developed foot, at this time, the shell length size was 320 360 $\mu\textrm{m}$.

• 한국동물학회지
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• 제30권3호
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• pp.277-291
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• 1987

틸보꼬마풀게 (Hemigrapsus sinensis Rathbun) (바위 게 과, 참게 아과)의 유생을 부추에서부터 첫번째 게퇘까지 수온 20.2$^{\circ}C$에서 24.1$^{\circ}C$, 염분농도 32,9도에서 33.2%o에서 사육하고, 각 유생기의 형태적인 특징을 기술 및 도시하여, 같은 속내에 보고된 다른 7종의 유생들과 kk교하였다. 이 종은 5기의 Zoea와 1기의 Megalopa 유생을 거쳐 첫번째 게탰로 변태하였다. 제 1 Zoea기의 제 1촉각과 미절이 Aikawa의 기준에 의한 공형이며 갑측극은 있고 제 2소악내지의 강량식이 2.2인 점에서는 같은 속의 다른 7종과 일치하나, 제 1악각의 기절 및 내파와 제 2악각의 내지의 강모식에 약간의 차리가있다.

• Animal cells and systems
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• 제6권2호
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• pp.107-123
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• 2002

One ovigerous crab of Hemigrapsus longitarsis (Miers, 1879) was collected in Jeju Island, Korea and their larvae were reared in the laboratory. Five zoeal and one megalopal stages are described and illustrated in detail. Morphology of the zoeas slightly differs from that in the previous record. Within the genus Hemigrapsus, H. longitarsis shows similarity closer to H. sanguineus and H. penicillatus than to H. sinensis based on the zoeal morphology. The zoeas of H. longitarsis can be distinguished from those of the two other species in having a dorsal carapace spine with minute spinules which is naked in H. sanguineus and H. penicillatus. A provisional key is provided to aid the identification of the grapsid zoeas in Korea.