• 제목/요약/키워드: Competitive Method

검색결과 1,294건 처리시간 0.036초

A Simple Method for Generation of Homologous Internal Standards for Competitive PCR

  • Choi, Eu-Na;Hahn, Sung-Sik;Choi, Kyung-Hee;Na, Doe-Sun
    • BMB Reports
    • /
    • 제29권5호
    • /
    • pp.481-483
    • /
    • 1996
  • In competitive PCR, which is used to quantify target DNA an internal standard is needed. Here we present a simple method to construct homologous competitive standards. The method, which is based upon deletion of a portion of the target DNA, does not require any additional primers. This is the simplest method developed thus far to construct a competitive standard. The whole procedure. from construction of a competitive standard to quantitation by PCR, can be completed within a single day.

  • PDF

컨테이너 항만간의 경쟁 상황을 고려한 물동량예측에 관한 연구 (A study on the forecasting of container cargo volumes in northeast ports by development of competitive model)

  • K.T.Yeo;Lee, C.Y.
    • 한국항해항만학회:학술대회논문집
    • /
    • 한국항해항만학회 1998년도 추계학술대회논문집:21세기에 대비한 지능형 통합항만관리
    • /
    • pp.263-269
    • /
    • 1998
  • The forecasting of container cargo volumes should be estimated correctly because it has a key roles on the establishment of port development planning, and the decision of port operating system. Container cargo volumes have a dynamic characteristics which was changed by effect of competitive ports. Accordingly forecasting was needed overall approach about competitive port's development, alternation and information. But, until now, traffic forecasting was not executed according to competitive situation, and that was accomplished at the point of unit port. Generally, considering the competition situation, simulation method was desirable at forecasting because system's scale was increased, and the influence power was intensified. In this paper, considering this situation, the objectives can be outlined as follows. 1) Structural model constructs by System dynamics method. 2) Structural simulation model develops according to modelling of competitive situation by expended SD method which included HEP(Hierarchical Fuzzy Process) And actually, effectiveness was verified according to proposed model to major port in northeast asia.

  • PDF

경쟁기법을 이용한 스튜어트 플랫폼의 순기구학 해석 (The Analysis of the Forward Kinematics Using the Competitive Method in the Stewart Platform)

  • 허성준;이형상;한명철
    • 한국정밀공학회:학술대회논문집
    • /
    • 한국정밀공학회 2001년도 춘계학술대회 논문집
    • /
    • pp.255-258
    • /
    • 2001
  • This introduces a improved method of the forward kinematics analysis, which finds the 6DOF motions and velocities from the given six cylinder lengths in the Steward platform. The numerical method(Newton Raphson Mehotd)of the forward kinematics analysises has the disadvantage of the long calculated time. To overcome this, we propose the competitive method that determine a proper initial value. Through the competitive method, we can select a proper initial value so that the calculate time is reduced. therefore we can give the property of the real time process to the forward kinematics analysis. We show the result comparing between general Newton-Raphson method and proposed one. From the result we verify the performance of the proposed method.

  • PDF

신뢰성기반 제품경쟁력 결정요인 선정방안 (Choice method of Competitive Power Key Factors based on Reliability)

  • 김종걸;빈성욱
    • 대한안전경영과학회:학술대회논문집
    • /
    • 대한안전경영과학회 2004년도 추계학술대회
    • /
    • pp.259-267
    • /
    • 2004
  • Enterprise must establish a properly competitive strategy according to environmental changes and international competitions. It is necessary to develop the strategy of competitive power for an efficient operation as well as system, industry, product and price competitions. This paper aims to propose a choice method of Competitive Power Key Factors which can be applied to unit parts and finished products, construction products, soft wear products based on Reliability.

  • PDF

MTS(마할라노비스-다구찌 시스템) 기법을 이용한 기업 품질경쟁력 측정 (The Study of Measure of Company Quality Competitive by using MTS Method)

  • 지철민;이상복
    • 품질경영학회지
    • /
    • 제33권2호
    • /
    • pp.64-73
    • /
    • 2005
  • In this paper, we introduce MTS(Mahalanobis-Taguchi System) Method which is suggested Dr. Taguchi in later 1990. We apply MTS Method for Quality Competitive Appraisal System(QCAS) Model which is executived from 1997 by Agency for Technology and Standards, Ministry of Commerce, Industry and Energy(MOCIE). We can measure company Quality competitive by using MTS. MTS Measure can not be compared statistical sum by calculated QCAS. MTS can be possible distinct subtle which can not distinct using statistical sum. Also MTS Method can seek more strong effect factor among of many factor. If A Company use MTS Method, can find vital factor and level of destination.

FSM법에 의한 항만경쟁력의 구조분석에 관한 연구 (A Study on the Structural Analysis of the Port Competition Power by FSM Method)

  • 여기태
    • 한국항해학회지
    • /
    • 제25권4호
    • /
    • pp.477-486
    • /
    • 2001
  • Although the ports are actually competing with various strategies, the definition and structural understanding of port competitive power are not known very much. Therefore this study has launched from this fact, and has the objective of obtaining the structural model of the competitive power, and understanding the components of the port competitive power. The following are the results of the study. First, the process began by abstracting the components that composed the port competitive power through recent research, and grouping it by the most core components using the KJ method. Also, by using the FSM(Fuzzy Structural Modeling) method to understand the structure of the grouped components, and the structural model of the port competitive power was able to obtain as the result. Second, when analyzing the obtained structural model, port expenses, main trunk location, port congestion and port facility came out to be the most important component groups, and especially port expenses was the most effective component that effected all the other components overall. Third, the component groups that were relatively less important, effected by most of the other components, and located on the top level of the structure model were the hinterland accessibility, port ownership, customs duties speed, and large ship port entrance possibility etc. Fourth, the results of this study will be able to be used when establishing competing strategies for our country's ports by proposing the relatively important components with the port competitive rower considered.

  • PDF

Competitive Spectrophotometry for Microbial Dipeptide Transport Systems

  • Hwang, Se-Young;Ki, Mi-Ran;Cho, Suk-Young;Lim, Wang-Jin;Yoo, Ick-Dong
    • Journal of Microbiology and Biotechnology
    • /
    • 제6권2호
    • /
    • pp.92-97
    • /
    • 1996
  • Portage kinetic constants of peptide transport can be measured by competitive spectrophotometry. The kinetic constants of L-Glu-L-Glu transport in Escherichia coli were ascertained using L-Phe-L-3-thia-Phe (PSP) as a detector. Since the production of thiophenol upon intracellular hydrolysis of PSP was competitively inhibited by L-Glu-L-Glu, it was able to compute the kinetic constants of L-Glu-L-Glu using this method. The resulted data were in agreement with the values obtained by the method of Michaelis-Menten kinetics. The potential of this method was examined against dipeptide transport systems in various microorganisms. These results strongly suggest that the overall properties of individual systems for dipeptide transports can be easily characterized by competitive spectrophotometry.

  • PDF

Economic Evaluation of Transmission Expansion for Investment Incentives in a Competitive Electricity Market

  • Fischer, Robert;Joo, Sung-Kwan
    • International Journal of Control, Automation, and Systems
    • /
    • 제6권5호
    • /
    • pp.627-638
    • /
    • 2008
  • With the shift of the electric power industry from a regulated monopoly structure to a competitive market environment, the focus of the transmission expansion planning has been moving from reliability-driven transmission expansion to market-based transmission expansion. In market-based transmission expansion, however, a growing demand for electricity, an increasing number of transmission bottlenecks, and the falling levels of transmission investment have created the need for an incentive to motivate investors. The expectation of profit serves as a motivational factor for market participants to invest in transmission expansion in a competitive market. To promote investment in transmission expansion, there is an increasing need for a systematic method to examine transmission expansion for investment incentives from multiple perspectives. In this paper, the transmission expansion problem in a competitive market environment is formulated from ISO and investors' perspectives. The proposed method uses parametric analysis to analyze benefits for investors to identify the most profitable location and amount for transmission addition. Numerical results are presented to demonstrate the effectiveness of the proposed method.

SD법과 HFP법의 융합을 이용한 항만경쟁모델의 개발 (Development of Competitive Port Model Using the Hybrid Mechanism of System Dynamic Method and Hierarchical Fuzzy Process Method)

  • 여기태;이철영
    • 한국시스템다이내믹스연구
    • /
    • 제1권1호
    • /
    • pp.103-131
    • /
    • 2000
  • If a system such as a port has a large boundary and complexity, and the system's substance is considered as a black box, forecast accuracy will be very low. Futhermore various components in a port exert significant influence on each other. To copy with these problem the form of structure models were introduced by using SD method. The Competitive Ports Model had several sub-systems consisting of each Unit Port models, and each Unit Port model was made by quantitative, qualitative factors and their feedback loops. The fact that all components of one port have influence on the components of the other ports should be taken into account to construct Competitive Port Models. However, with the current approach that is impossible, and in this paper therefore, models were simplified by HFP adapted to integrate level variables of unit port models. Although many studies on modelling of port competitive situation have been conducted, both theoretical frame and methodology are still very weak. In this study, a new algorithm called ESD(Extensional System Dynamics) for the evaluation of port competition was presented, and applied to simulate port systems in northeast asia.

  • PDF

ELISA 방법을 이용한 요중 아플라톡신 M1 측정 (Application of Competitive ELISA Method for Estimation of Urinary Aflatoxin M1 Level)

  • 김용대;김헌
    • 생명과학회지
    • /
    • 제23권2호
    • /
    • pp.306-310
    • /
    • 2013
  • 본 연구는 요중 아플라톡신 M1 (AFM1)의 농도를 측정할 수 있는 competitive ELISA 방법의 특성을 HPLC-fluorescence detector (HPLC-FLD) 방법과 비교하여 평가하였다. ELISA 방법에서의 AFM1의 회수율은 105% (73-124%)였고 측정의 변이계수는 6.85%로 나타났다. ELISA 방법에서의 검출한계와 정량한계는 각각 0.20 pg/ml과 0.62 pg/ml로 조사되었으며, 두 방법을 이용하여 측정한 요중 AFM1 농도는 상관계수 0.96의 매우 높은 상관성이 있는 것으로 확인되었다(p<0.01). 그러나, 이렇게 높은 상관성에도 불구하고, ELISA 방법을 이용한 요중 AFM1의 농도는 HPLC-FLD 방법으로 측정한 값에 비해 상대적으로 높게 나타나는 경향을 보여 ELISA를 이용한 방법이 단시간에 많은 시료를 분석할 수 있는 장점은 있으나 그 결과는 HPLC-FLD 방법을 이용해서 얻은 회귀식을 이용하여 보정을 한 후 제시할 필요가 있는 것으로 판단된다.