• Journal of the Society of Naval Architects of Korea
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• v.31 no.4
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• pp.23-31
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• 1994

The compartmentation model is represented by the characteristics and geometric information of the spaces defined by the structural members which are used for the boundary planes of the compartment. For the efficient compartment modeling a program performing the compartmentation design by the chopping and merging method was presented by authors. In this research, the development of an interactive ship compartmentation modeller is introduced. It is natural that the value of the program lessens if the input process is complicated and uneasy, even though the internal techniques for the compartmentation modeling are superior. In this paper, a method for the convenient input is proposed and implemented with the help of a graphical user interface technique. The modeling method introduced in this paper performs an efficient compartmentation modeling fast and conveniently by the solid modeling concept and the graphical user interface.

• The Microorganisms and Industry
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• v.15 no.2
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• pp.8-12
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• 1989

• Journal of the Society of Naval Architects of Korea
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• v.30 no.3
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• pp.16-28
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• 1993

The object-oriented paradigm is being recognized as the most effective technique to develope the complex, large software system. In this paper, we propose the modeling methodology using object-oriented technology for the ship compartmentation design(OO _COMDEF) based on the proposed modeling methodology. Illustrative examples to show the application of the prototype system are given.

• Proceedings of the Korean Biophysical Society Conference
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• 2003.06a
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• pp.29-29
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• 2003

Compartmentation of intracellular signaling pathways serves as an important mechanism conferring the specificity of G protein-coupled receptor (GPCR) signaling. In the heart, stimulation of $\beta$$_2$-adrenoceptor ($\beta$$_2$-AR), a prototypical GPCR, activates a tightly localized protein kinase A (PKA) signaling, which regulates substrates at cell surface membranes, bypassing cytosolic target proteins (eg, phospholamban). Although a concurrent activation of $\beta$$_2$-AR-coupled $G_{i}$ proteins has been implicated in the functional compartmentation of PKA signaling, the exact mechanism underlying the restriction of the $\beta$$_2$-AR-PKA pathway remains unclear. In the present study, we demonstrate that phosphatidylinositol 3-kinase (PI3K) plays an essential role in confining the $\beta$$_2$-AR-PKA signaling. Inhibition of PI3K with LY294002 or wortmannin enables $\beta$$_2$-AR-PKA signaling to reach intracellular substrates, as manifested by a robust increase in phosphorylation of phospholamban, and markedly enhances the receptor-mediated positive contractile and relaxant responses in cardiac myocytes. These potentiating effects of PI3K inhibitors are not accompanied by an increase in $\beta$$_2$-AR-induced cAMP formation. Blocking $G_{i}$ or $G_{$\square$$\square$}$ signaling with pertussis toxin or $\beta$ARK-ct, a peptide inhibitor of $G_{$\square$$\square$}$, completely prevents the potentiating effects induced by PI3K inhibition, indicating that the pathway responsible for the functional compartmentation of $\beta$$_2$-AR-PKA siglaling sequentially involves $G_{i}$, $G_{$\square$$\square$}$, and PI3K. Thus, PI3K constitutes a key downstream event of $\beta$$_2$-AR- $G_{i}$ signaling, which confines and negates the concurrent $\beta$$_2$-AR/Gs-mediated PKA signaling.gnaling.

• Journal of the Society of Naval Architects of Korea
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• v.29 no.4
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• pp.45-57
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• 1992

In this paper, the trends for the standardization of the product model was surveyed, and the product model can be used as concrete means to realize the communication of information between the CAD/CAM softwares, which are used for the design and manufacturing of the complex products such as ships. We have proposed the newly developed methodology for the representation and definition of the ship model on a basis of the product model. And also we have studied the product modeling technology for the aspect of an application, and the object-oriented system technology have been surveyed for the system implementation issues. We would like to verify the consistency and correctness of our proposed representation methodology by using the prototype application model, which is applied to design work of ship compartmentation model. For this purpose we have developed the "OO_COMDEF"(which means the Object-Oriented System for the Compartmentation Definition of ship) program that is applied to the compartmentation model which can be considered as a submodel of the general ship models. The results of research work have been proved that the representation methodology for the ship model based on the product model is an efficient and appropriate scheme for the ship model definitions. Consequently, this methodology can be proposed as a fundamental framework for the development of the shipbuilding CAD/CAM system.

• Proceedings of the Korea Institute of Fire Science and Engineering Conference
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• 2000.11a
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• pp.160-165
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• 2000

• Korean Journal of Plant Tissue Culture
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• v.27 no.4
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• pp.269-282
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• 2000

Benzylisoquinoline alkaloids are a diverse group of natural products that include many pharmacologically active compounds produced in a limited number of plant families. Despite their complexity, intensive biochemical research has extended our knowledge of the chemistry and enzymology of many important benzylisoquinoline alkaloid pathways, such as those leading to the analgesic drugs morphine and codeine, and the antibiotics sanguinarine and berberine. The use of cultured plant cells as an experimental system has facilitated the identification and characterization of more than 30 benzylisoquinoline alkaloid biosynthetic enzymes, and the molecular cloning of the genes that encode at least 8 of these enzymes. The recent expansion of biochemical and molecular technologies has creat-ed unique opportunities to dissect the mechanisms involved in the regulation of benzylisoquinoline alkaloid biosynthesis in plants. Research has suggested that product accumulation is controlled by the developmental and inducible regulation of several benzylisoquinoline alkaloid biosynthetic genes, and by the subcellular compartmentation of biosynthetic enzymes and the intracellular localization and trafficking of pathway intermediates. In this paper, we review our current understanding of the biochemistry, cell biology, and molecular regulation of benzylisoquinoline alkaloid biosynthesis in plants. We also summarize our own research activities, especially those related to the establishment of protocols for the genetic transformation of benzylisoquinoline alkaloid-producing species, and the development of metabolic engineering strategies in these plants.

• Journal of Plant Biotechnology
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• v.7 no.1
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• pp.1-15
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• 2005

Salinity is one of the major limiting factors for agricultural productivity. In plants, accumulation of osmolytes plays a pivotal role in abiotic stress tolerance. Likewise, exclusion or compartmentation of $Na^+$ ions into vacuoles provides an efficient mechanism to avert deleterious effects of $Na^+$ in the cytosol. Both vacuolar and plasma membrane sodium transporters and $H^+-ATPases$ can provide the necessary ion homeostasis. A variety of crop plants were engineered with respect to the synthesis of osmoprotectants and ion-compartmentation, but there are other cellular pathways involved in the salinity responses that are still not completely explored. Genomics approaches are increasingly used to identify genes and pathway changes involved in salt-tolerance. The new knowledge may be used via guided genetic engineering of multiple genes to create crop plants with significantly increased productivity in saline soils. This review surveys how plants deal with high salt conditions and how salt tolerance can be improved by transgenic approaches.

• Biomedical Science Letters
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• v.11 no.3
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• pp.301-305
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• 2005

MR spectroscopy of intracellularly located $^{133}Cs$ has been used to monitor the uptake of Gd-EOB-DTPA by the isolated rat liver. As shown by ${31}P$ spectroscopy, accumulation of $^{133}Cs$ ions in hepatocytes does not produce detectable effects on the metabolism. The hepatic internalization of Gd-EOB-DTPA was followed by the paramagnetic relaxation enhancement of the intracellular $^{133}Cs$ ions, and confirmed by parallel quantitations of Gd and Cs run by inductively coupled plasma analysis of liver samples and aliquots of perfusate. Two peaks are observed at -22.0 and -23.5 ppm, with respect to the line of the external reference arbitarily set to 0 ppm. Upon rinsing of the extracellular compartment with regular K-H free of CsCl, the high-field resonance disappears within 20min. The intracellular concentration was confirmed by ICP, which gives a $Cs^+$ content of $22.0\pm3.5mM$. The relaxation data significantly underestimate the Gd content, suggesting a potential compartmentation of $Cs^+$ and the contrast agent.

• Proceedings of the Korea Institute of Fire Science and Engineering Conference
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• 2010.04a
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• pp.354-359
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• 2010

최근 성능기준에 의한 평가규정이 시행된 건축물 내화충전구조에 대해 관련 제도운영체계와 국내외 성능평가방법 및 성능기준을 살펴본 후 현재 기준의 보완점과 향후 개선방안에 대해 고찰하였다. 내화충전구조는 방화구획의 기능을 완성시키는 중요한 부분으로서 연구개발 및 시장활성화가 필요하며, 현장 품질을 확보 유지할 수 있는 관리체계가 필요하다. 한편 내화성능 뿐만 아니라 구조거동에 대한 대응 및 내구성에 대한 기술기준의 보완 마련이 필요하다.