• Mycobiology
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• 제47권2호
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• pp.230-241
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• 2019

The Great Sebkha of Oran is a closed depression located in northwestern of Algeria. Despite the ranking of this sebkha among the wetlands of global importance by Ramsar Convention in 2002, no studies on the fungal community in this area have been carried out. In our study, samples were collected from two different regions. The first region is characterized by halophilic vegetation and cereal crops and the second by a total absence of vegetation. The isolated strains were identified morphologically then by molecular analysis. The biotechnological interest of the strains was evaluated by testing their ability to grow at different concentration of NaCl and to produce extracellular enzymes (i.e., lipase, amylase, protease, and cellulase) on solid medium. The results showed that the soil of sebkha is alkaline, with the exception of the soil of cereal crops that is neutral, and extremely saline. In this work, the species Gymnoascus halophilus, Trichoderma gamsii, the two phytopathogenic fungi, Fusarium brachygibbosum and Penicillium allii, and the teleomorphic form of P. longicatenatum observed for the first time in this species, were isolated for the first time in Algeria. The halotolerance test revealed that the majority of the isolated are halotolerant. Wallemia sp. and two strains of G. halophilus are the only obligate halophilic strains. All strains are capable to secrete at least one of the four tested enzymes. The most interesting species presenting the highest enzymatic index were Aspergillus sp. strain A4, Chaetomium sp. strain H1, P. vinaceum, G. halophilus, Wallemia sp. and Ustilago cynodontis.

• 한국미생물·생명공학회지
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• 제47권1호
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• pp.105-115
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• 2019

Saline or alkaline condition in soil inhibits growth of most crop plants and limits crop yields in many parts of the world. Augmenting an alkaline soil with alkali-tolerant bacteria capable of promoting plant growth can be a promising approach in expanding fertile agricultural land. Near-shore environments of Dokdo Island, a remote island located in the middle of the East Sea, appear to have patches of seawater-influenced haloalkaline soil that is unsupportive for growth of conventional plants. To exploit metabolic capacities of alkali-tolerant bacteria for promoting plant growth in saline or alkaline soils, we isolated of alkali-tolerant bacteria from near-shore soil samples in Dokdo and investigated properties of the isolates. Alkali-tolerant bacteria were selectively cultivated by inoculating suspended and diluted soil samples on a plate medium adjusted to pH 10. Fifty colonies were identified based on their $GTG_5$-PCR genomic fingerprints and 16S rRNA gene sequences. Most isolates were affiliated to alkali-tolerant and/or halotolerant genera or species of the phyla Firmicutes (68%), Proteobacteria (30%) and Actinobacteria (2%). Unlike the typical soil bacterial flora in the island, alkali-tolerant isolates belonged to only certain taxa of terrestrial origin under the three phyla, which have traits of plant growth promoting activities including detoxification, phytohormone production, disease/pest control, nitrogen-fixation, phosphate solubilization or siderophore production. However, Firmicutes of marine origin generally dominated the alkali-tolerant community. Results of this study suggest that haloalkaline environments like Dokdo shore soils are important sources for plant growth promoting bacteria that can be employed in bio-augmentation of vegetation-poor alkaline soils.

• 한국물환경학회지
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• 제35권2호
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• pp.152-164
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• 2019

In this study, a Benthic Macroinvertebrates Family Index (BMFI) was developed using 100 indicator groups (99 families including Chironomidae with 2 phena). Families were assigned a score between 1 and 10 depending on their sensitivity to organic pollution. The BMFI was composed of the sensitivity and relative abundance of the indicator taxa. Sensitivity values of each group were generally similar to Biological Monitoring Working Party (BMWP) scores or Walley, Hawkes, Paisley, Trigg (WHPT) scores of UK, Japanese BMWP scores, and the FBI tolerance values of North America. However, sensitivity values of some taxa were significantly different from those of foreign countries, which seemed to have resulted from discrepancy in species composition, difference of taxonomic classification system, or methodological difference for estimation of sensitivity. As an annual average level, BMFI showed significant correlation with concentration of 5-day biochemical oxygen demand (BOD5) (correlation coefficient r = -0.80, n = 569 sites), total suspended solids (r = -0.68), and total phosphorus (r = -0.79). In addition, BMFI revealed strong correlation with Shannon-Weaver's species diversity (r = 0.85), Margalef's species richness (r = 0.85) and McNaughton's dominance (r = -0.84). Correlation between BMFI and water quality parameters or community indices such as species diversity did not show significant difference compared to that of species-level indices such as BMI (Benthic Macroinvertebrates Index). This means that BMFI is a more useful indicator in terms of easy identification of organisms. BMFI was used to assess the environmental status of 3,017 sites of Stream Ecosystem Survey conducted by the Korean Ministry of Environment between 2016 and 2018. As a result, about half of all sites appeared to be in good condition, and a quarter in poor condition.

• 한국미생물·생명공학회지
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• 제49권3호
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• pp.432-439
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• 2021

Microbial community plays important roles in the culture environment of mud crab Scylla serrata. One of the environmental management efforts for the cultivation of S.serrata is by stabilizing microorganisms involved in nitrogen cycle process. The availability of dissolved inorganic nitrogen in its culture environment under a recirculating system closely relates to the nitrogen cycle, which involves both anaerobic and aerobic bacterial activities. Anaerobically, there are two major nitrogen compound degradation processes, i.e., denitrification and dissimilatory nitrate reduction to ammonium (DNRA). This study aimed to identify denitrifying and DNRA bacteria isolated from the recirculating cultivation of S. serrata. The water samples were collected from anaerobic filters called close filter system, which is anaerobically conditioned with the addition of varying physical filter materials in the recirculating mud crab cultures. The results showed that three denitrifying bacterial isolates and seven DNRA bacterial isolates were successfully identified. The phylogenetic analysis based on 16S rRNA gene of the denitrifying bacteria revealed that HIB_7a had the closest similarity to Stenotrophomonas daejeonensis strain MJ03. Meanwhile, DNRA bacterial isolate of HIB_92 showed a 100% similarity to Bacillus sonorensis strain N3, Bacillus vallismortis strain VITS-17, Bacillus tequlensis strain TY5, Geobacillus sp. strain DB24, Bacillus subtilis strain A1, and Bacillus mojavensis strain SSRAI21. This study provides basic information denitrifying and DNRA bacterial isolates identity which might have the potential to be applied as probiotics in aquaculture systems in order to maintain optimal environmental conditions.

• 한국해양학회지:바다
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• 제26권3호
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• pp.238-247
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• 2021

돌말류는 미세조류의 일종으로서 담수 및 해양의 여러 다양한 환경의 수생태계에서 가장 중요한 1차 생산자 중 하나이다. 환경을 대표할 수 있는 생물 지표로서 널리 이용되고 있으며, 여러 지역에서 서로 다른 과학자들에 의한 연구 결과들이 과학적 통일성과 객관성을 갖추도록 하기 위해서는 돌말류 분석 자료의 정도 관리가 매우 중요하다. 현재 돌말류 분석은 형태적인 특징으로 분류하는 형태 기반의 분석과 DNA 서열로 종을 식별하는 DNA 바코드 분석이 사용되고 있다. 형태학 기반의 돌말류 분류는 분석 자료를 해석하는 분류학자들 간의 일관된 종 식별이 요구되는 한편 분자 분류 기반의 돌말류 분류는 신뢰성 있는 참조데이터가 필수적이다. 본 논문에서는 돌말류 분석 자료 정도관리의 국내외 현황을 우선 살펴보았으며, 그에 기반하여 국내 돌말류 분석 자료의 정도 관리에 대한 의견을 또한 제안하였다.

• 한국농공학회논문집
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• 제63권4호
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• pp.23-32
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• 2021

For agricultural water management, it is essential to establish the digital infrastructure data such as agricultural watershed, irrigated area and canal network in rural areas. Approximately 70,000 irrigation facilities in agricultural watershed, including reservoirs, pumping and draining stations, weirs, and tube wells have been installed in South Korea to enable the efficient management of agricultural water. The total length of irrigation and drainage canal network, important components of agricultural water supply, is 184,000 km. Major problem faced by irrigation facilities management is that these facilities are spread over an irrigated area at a low density and are difficult to access. In addition, the management of irrigation facilities suffers from missing or errors of spatial information and acquisition of limited range of data through direct survey. Therefore, it is necessary to establish and redefine accurate identification of irrigated areas and canal network using up-to-date high resolution images. In this study, previous existing data such as RIMS (Rural Infrastructure Management System), smart farm map, and land cover map were used to redefine irrigated area and canal network based on appropriate image data using satellite imagery, aerial imagery, and drone imagery. The results of the building the digital infrastructure in rural areas are expected to be utilized for efficient water allocation and planning, such as identifying areas of water shortage and monitoring spatiotemporal distribution of water supply by irrigated areas and irrigation canal network.

• 동의생리병리학회지
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• 제35권1호
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• pp.42-46
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• 2021

The purpose of this study was to investigate the relationship between sleep quality and sleep duration according to individual characteristics, and the relationship between sleep quality and cold type. A total of 1998 participants aged 30-55 who participated in a community cohort study at KIOM's Oriental Medicine Data Center (KDC) was analyzed. Cold type was diagnosed with a common the Questionnaire of Cold Pattern Identification (8 items). Sleep quality was assessed by the Pittsburgh Sleep Quality Index. The participants in this study were classified into 1193 with cold type and 805 with non-cold type. The poor sleep quality was found in more women than men, and the Body Mass Index (BMI) was higher in the non-cold type. In the cold type group, average sleep quality was poor and sleep duration was short. There was a statistically significant correlation between cold score, sleep quality, and sleep duration. Cold type was related to the sleep quality. The risk of poor sleep quality increased 1.46 with increased cold type (95% CI = 1.16-1.84, p <.001). In particular, it was found that various programs to improve the quality of sleep, focusing on the cold type among personal characteristics, are needed to maintain adequate sleep to improve the sleep quality. In the future, when analyzing the relationship between cold type and sleep quality, it is thought that an attempt to derive other objective quantitative indicators is needed.

• 한국임상약학회지
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• 제30권4호
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• pp.270-278
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• 2020

Background: Although the identification of clinical and laboratory features in pediatric COVID-19 patients is essential in establishing an appropriate treatment plan, a systematic review and meta-analysis on the topic has yet to be reported. Methods: We searched MEDLINE, Embase, and Web of Science to access clinical and laboratory characteristics as well as clinical outcomes of children with COVID-19 infection. A meta-analysis using random-effect model was performed to estimate pooled prevalence and 95% confidence intervals. Results: Among the 532 studies initially collected, 12 articles were finally included in the meta-analysis. Among the investigated 320 pediatric patients with COVID-19, fever (48.2%) and cough (39.3%) were the most common symptoms. Almost one third of patients (30.4%) were asymptomatic. In laboratory findings, only 11.4% of pediatric patients experienced lymphocytopenia. Increased inflammatory markers including c-reactive protein (18.6%) and procalcitonin (32.4%) were observed. Only a few patients needed mechanical ventilation and intensive care support, and only one death was reported. Conclusion: Pediatric patients with COVID-19 infection exhibited milder symptoms and more favorable outcomes compared to adults. However, considering the high rate of asymptomatic pediatric patients, close monitoring is required to prevent community infection in asymptomatic conditions and hidden disease progression.

• 한국농공학회논문집
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• 제64권1호
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• pp.51-63
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• 2022

Up-to-date statistics of crop cultivation status is essential for farm land management planning and the advancement in remote sensing technology allows for rapid update of farming information. The objective of this study was to develop a classification model of rice paddy or winter crop fields based on NDWI, NDVI, and HSV indices using Sentinel-2 satellite images. The 18 locations in central Korea were selected as target areas and photographed once for each during summer and winter with a eBee drone to identify ground truth crop cultivation. The NDWI was used to classify summer paddy fields, while the NDVI and HSV were used and compared in identification of winter crop cultivation areas. The summer paddy field classification with the criteria of -0.195

• International Journal of Computer Science & Network Security
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• 제22권2호
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• pp.63-74
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• 2022

Repressive measures in the Crimea against the Ukrainian media and the ban on the entry of international and Ukrainian monitoring missions created the conditions for the function of providing information to be performed by representatives of civil society. Such a phenomenon was called Crimean citizen journalism and became a post-occupation phenomenon characteristic of the Crimean information sphere. The journalists' activities are aimed at reporting on human rights violations and repression against Ukrainian citizens who find themselves in conditions of information bans and restrictions. Crimean citizen journalism, which connects the peninsula with the mainland of Ukraine, is monothematic in nature, and its emergence has become a form of nonviolent resistance to the occupation of Crimea. The purpose of the study is to cover the characteristic features, the development of common Crimean citizen journalistic movement features as a social phenomenon, a phenomenon that arose after the occupation through the identification of a modern journalist portrait. The study uses the general scientific method of empirical research as the main one, the sociological method of a questionnaire survey, as well as the methods of classification, generalisation, observation, statistical calculation. An analysis of a survey of Crimean citizen journalists demonstrates the existence of an active, mobile community in Crimea that seeks to provide information and human rights nonviolent resistance to the occupation.