• Fisheries and Aquatic Sciences
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• 제18권1호
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• pp.99-107
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• 2015

A natural hybrid of a probable intergeneric mating between the striped shiner Pungtungia herzi and the stone morocco Pseudorasbora parva (Cypriniformes: Cyprinidae) was captured in the Geumho River, a tributary of the Nakdong River basin in Korea. Morphological characters and DNA sequences were analyzed to verify its hybrid state and identify the parentage of its parent species. The hybrid exhibited a phenotypic intermediacy between the two parent species in the number of vertebrae and the mouth shape. Out of 1,488 base pair (bp) positions of the nuclear recombination activating gene 1 gene (rag1), which has a biparental mode of inheritance, 41-bp substitutions were detected between the two parent species, whereas an electropherogram of the hybrid displayed polymorphic double peaks at all of the base positions, along with one additional one, strongly indicating its hybrid state. Meanwhile, sequence comparison of the mitochondrial cytochrome b gene (mt-cyb) (1,140 bp), which has a maternal mode of inheritance, showed only 5-22-bp differences (97.6-99.5% identities) between the hybrid and Pu. herzi, but as many as 158-168-bp differences (85.2-86.1% identities) between the hybrid and Ps. parva, clearly indicating Pu. herzi as the maternal species. Thus, combined analyses of independent data sets (i.e., morphology and nuclear and mitochondrial DNA sequences) offered convincing evidence for the hybrid state of a naturally occurring hybrid resulting from intergeneric mating between a female Pu. herzi and a male Ps. parva.

• BMB Reports
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• 제40권3호
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• pp.373-381
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• 2007

Seed storage proteins (SSPs) are important for seed germination and early seedling growth. We studied the accumulation and degradation profiles of four major Arabidopsis 12S SSPs using a 2-DE scheme combined with mass spectrometric methods. On the 2-DE map of 23 dpa (days post anthesis) siliques, 48 protein spots were identified as putative full-length or partial $\alpha$, $\delta$ subunits. Only 9 of them were found in 12 dpa siliques with none in younger than 8 dpa siliques, indicating that the accumulation of 12S SSPs started after the completion of cell elongation processes both in siliques and in developing seeds. The length and strength of transcription activity for each gene determined the final contents of respective SSP. At the beginning of imbibition, 68 SSP spots were identified while only 2 spots were found at the end of the 4 d germination period, with $\alpha$, subunits degraded more rapidly than the $\alpha$ subunits. The CRC $\delta$ subunit was found to degrade from its C-terminus with conserved sequence motifs. Our data provide an important basis for understanding the nutritional value of developing plant seeds and may serve as a useful platform for other species.

• Parasites, Hosts and Diseases
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• 제54권6호
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• pp.777-786
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• 2016

Raillietina species are prevalent in domestic chickens (Gallus gallus domesticus) in Phayao province, northern Thailand. Their infection may cause disease and death, which affects the public health and economic situation in chicken farms. The identification of Raillietina has been based on morphology and molecular analysis. In this study, morphological observations using light (LM) and scanning electron microscopies (SEM) coupled with molecular analysis of the internal transcribed spacer 2 (ITS2) region and the nicotinamide adenine dinucleotide dehydrogenase subunit 1 (ND1) gene were employed for precise identification and phylogenetic relationship studies of Raillietina spp. Four Raillietina species, including R. echinobothrida, R. tetragona, R. cesticillus, and Raillietina sp., were recovered in domestic chickens from 4 districts in Phayao province, Thailand. LM and SEM observations revealed differences in the morphology of the scolex, position of the genital pore, number of eggs per egg capsule, and rostellar opening surface structures in all 4 species. Phylogenetic relationships were found among the phylogenetic trees obtained by the maximum likelihood and distance-based neighbor-joining methods. ITS2 and ND1 sequence data recorded from Raillietina sp. appeared to be monophyletic. The query sequences of R. echinobothrida, R. tetragona, R. cesticillus, and Raillietina sp. were separated according to the different morphological characters. This study confirmed that morphological studies combined with molecular analyses can differentiate related species within the genus Raillietina in Thailand.

• ALGAE
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• 제30권1호
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• pp.1-13
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• 2015

A new macroscopic riverine red algal species, Lemanea manipurensis sp. nov. (Batrachospermales) is described from Manipur in northeast India. It has a sparsely branched, pseudoparenchymatous thallus with a single, central axial filament that lacks cortical filaments. Spermatangia occur generally in isolated, low and indistinct patches or form an almost continuous ring around the axis. Carposporophytes project into the hollow thallus cavity without an ostiole. The most striking morphological feature is the carposporophyte with very short gonimoblast filaments having cylindrical, narrow and sparsely branched sterile filaments, the terminal cell of each branch with a single, large, elongate carpospore. The widely distributed L. fluviatilis has spherical carpospores in long branched chains. Phylogenetic analysis of rbcL sequence data and comparison with other Batrachospermales clearly show that our specimens do not align with other species of Lemanea and Paralemanea investigated thus far. Five specific names attributed in previous literature (1973-2014) to Lemanea from Manipur, L. australis, L. catenata, L. fluviatilis, L. mamillosa, and L. torulosa are rejected until critical anatomical and molecular evidence is available for specimens from the Manipur river systems. Taxa referable to Paralemanea were not confirmed for India in this study. In view of the high demand for food and medical uses of L. manipurensis in northeast India, conservation measures are needed for its long term survival. The present paper constitutes the first combined morphological / molecular study on a freshwater red alga from India.

• The Plant Pathology Journal
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• 제35권6호
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• pp.553-563
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• 2019

Investigations related with factors influencing root and crown rot are rare and mainly related to farming practice and soil management. The main objective of this study was to examine broader range of factors influencing stem-base infestation of winter wheat in the field conditions. The effect of spatial distribution of infected plants on disease index (DIs) assessments was also investigated. Analysis of factors influencing DIs of crown rot of wheat demonstrated significant influence of the growing seasons (P < 0.001) and extreme fluctuations in winter temperatures (P < 0.001). In addition to that, localities together with their interaction with the growing season also significantly influenced DIs (P < 0.001). Aggregation of infected plants influenced variability of DI estimations, and it was pointed out that more extensive investigation should be conducted on broad range of DI in order to establish sampling method giving uniform sampling precision. Fusarium graminearum was shown to be predominant Fusarium species in Serbia (72.6%) using sequence-characterized amplified region analysis. Interestingly F. oxysporum was isolated in higher frequencies (27.4%) than it was reported in the literature. Given that there were no reports on the diversity of Fusarium species causing crown rot of wheat in Serbia, this study presents first report on this important subject. It also indicated that more attention should be focused on combined effects of abiotic and biotic factors influencing stem-base infestation of winter wheat. This knowledge will contribute to better understanding of factors influencing root and crown rot of wheat which would ensure sustainable disease management in the future.

• 대한임베디드공학회논문지
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• 제8권4호
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• pp.185-194
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• 2013

Abstract- In distant-talking environments, speech recognition performance degrades significantly due to noise and reverberation. Recent work of Michael L. Selzer shows that in microphone array speech recognition, the word error rate can be significantly reduced by adapting the beamformer weights to generate a sequence of features which maximizes the likelihood of the correct hypothesis. In this approach, called Likelihood Maximizing Beamforming algorithm (Limabeam), one of the method to implement this Limabeam is an UnSupervised Limabeam(USL) that can improve recognition performance in any situation of environment. From our investigation for this USL, we could see that because the performance of optimization depends strongly on the transcription output of the first recognition step, the output become unstable and this may lead lower performance. In order to improve recognition performance of USL, some post-filter techniques can be employed to obtain more correct transcription output of the first step. In this work, as a post-filtering technique for first recognition step of USL, we propose to add a Wiener-Filter combined with Feature Weighted Malahanobis Distance to improve recognition performance. We also suggest an alternative way to implement Limabeam algorithm for Hidden Markov Network (HM-Net) speech recognizer for efficient implementation. Speech recognition experiments performed in real distant-talking environment confirm the efficacy of Limabeam algorithm in HM-Net speech recognition system and also confirm the improved performance by the proposed method.

• 한국컴퓨터정보학회논문지
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• 제19권8호
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• pp.119-127
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• 2014

본 논문에서는 편마비 환자는 정상인과 다른 보행 특성을 가지므로 본 논문에서는 이들의 특성을 반영하여 보행자세를 측정하고 실시간 3차원 그래픽으로 보여주는 시스템을 개발하였다. 환자는 허벅지, 종아리 및 발에 각각 센서 모듈을 착용하며, 측정 정밀도를 높이기 위하여 각 모듈에는 3축 가속도 센서와 3축 자이로 센서를 조합하여 적용하였다. 보행자세 디스플레이를 위하여 센서 모듈들로부터 수신한 데이터를 활용하여 허벅지, 종아리 및 발을 3차원 모형으로 모델링하여 실시간으로 화면에 보여주도록 하였으며, 편마비 환자의 특이한 보행 자세를 분석하기에 편리하도록 사용자의 보는 각도를 임의로 변화시킬 수 있도록 하였다. 또한 측정된 자세 정보를 활용하여 RLA(Rancho Los Amigos) 보행 주기의 단계별 진행되는 과정을 실시간으로 판단하여 화면에 표시할 수 있도록 함으로써 진행 단계 및 단계별 소요시간을 통하여 보행의 특성을 평가할 수 있도록 하였다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 1999년도 제17회 학술발표회 논문개요집
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• pp.203-203
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• 1999

Mass spectrometry technique provides the molecular weight distribution, data on the sequence of repeat units, polymer additives, and impurities, and structural information. time-of-Flight secondary Ion Mass Spectrometry (TOF-SIMS) has been used for structural characterization of various polymers1-2. the masses of repeat units and terminal groups and molecular weight distributions of polymers have been determined from their TOF-SIMS spectra. TOF-SMIS provides good sensitivity and structural specificity for high mass ions so that intact oligomers and large polymer fragments are observed. In this study, we investigated the detailed structural information on the oligomers and fragment ions of branched poly(1,3-butylene adipate) and branched poly[di(ethylene glycol) adipate] and the transesterification products of branched polyesters with trifluoroacetic acid or chloro difluoroacetic acid. Branched polyesters were chosen because they are important polymers but difficult to characterize; thus branched polyesters provide challanging test for TOF-SIMS. TOF-SIMS spectra of polyesters are obtained from thin polymer films cast from solution on a silver substrate. A good solvent for a polumer solution disrupts intermolecular forces between polymer chains but leaves the polumer intact. Transesterification reactions are potentially useful for characterization of high molecular weight and intractable polyesters. Transesterification products of polyesters and trifluoroacetic acid or an integral number of polyester repeat units and an additional diol. The progress of such reactions was monitored using peak intensities of reactants and products in TOF-SIMS spectra. The increasing abundance of tagged ions indicates that the reaction has progressed with time.

• 컴퓨터교육학회논문지
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• 제14권6호
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• pp.31-39
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• 2011

정보통신환경에는 관련지식이 부족하고 가치관도 정립되지 못한 초등학생들에게 특히 큰 위험요소가 상존한다. 본 논문에서는 초등 학생들의 정보통신윤리 수준을 개선시키고자 학생들이 더욱 많이 참여하고 흥미도 느낄 수 있는 사례 기반의 다중형 교수학습모형을 제안한다. 제안 모형은 실제 생활속에서 발생할 수 있는 정보통신윤리 갈등상황을 중심으로 진행된다. 수업은 사례기반으로 진행하기 적합하도록 세 종류의 교수학습모형(가치 갈등, 가치 명료화, 역할놀이 교수학습모형)을 통합하여 적용한다. 적용 후, 제안모형이 정보통신윤리 수준 향상에 미치는 교육적 효과를 차시별로 비교 분석한다. 검증은 평가지와 설문방법을 통하여 인지적, 정의적, 행동적, 종합적인 영역의 4가지로 나누어 실시한다. 연구결과, 제안모형이 학생들의 정보통신 윤리수준을 향상시키는데 긍정적인 효과가 있음을 보이고 있다.

• 복식
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• 제62권6호
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• pp.200-217
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• 2012

The purpose of this research is to examine the specificity of grids to define the characteristics of clothes styles in the Joseon Dynasty period. The significance of examining of the specificity of grids is to find out arbitrary types of the features of grids involved in structuring the Jeogori in the Joseon Dynasty period one by one. The Visual Linguistic Theory was introduced as a methodological tool to exquisitely analyze the characteristics of grids in deep structures of Jeogori in the Joseon Dynasty period. This theory strives to examine sample distribution, the distribution of samples by quality and the distribution of the types of ploidy features. Through the examination, the results are as follows. The grid systems of the Jeogori consisted of diverse proportion systems reaching 86 cases, that is, sequence systems composed of multi-functional, multi-combined bodies. Most ornamental grids had feature angles distributed in a range of $2-20^{\circ}$ that showed a common preference for low sloped diagonal lines or small curvature. Although the preference for certain feature angles were prominent, the feature angles that were used were generally distributed evenly among diverse feature angles to show the characteristics of separation. Therefore, Jeogori makers in the Joseon Dynasty period can be considered as having experimented with many proportion systems to show their aesthetics. In conclusion, based on the results of the examination of feature distributions and related methods to allocate ploidy features, O-type accounted for 66% and thus it was identified that the Jeogori was characterized by O-type. Therefore, it was identified that the characteristic of the Jeogori in the Joseon Dynasty period consisted of O-type fractal structures which are formative structures unique to our nation.