• Genomics & Informatics
• /
• 제18권4호
• /
• pp.37.1-37.11
• /
• 2020

BET inhibitor, as an epigenetic regulator inhibitor, reduces the expression of oncogenes such as Myc and Bcl-2, which affects cancer growth and development. However, it has modest activity because of the narrow therapeutic index. Therefore, combination therapy is necessary to increase the anti-tumor effect. Paclitaxel, an anti-mitotic inhibitor, is used as second-line therapy for gastric cancer (GC) as a monotherapy or combination. In this study, we performed RNA sequencing of GC cells treated with iBET-151 and/or paclitaxel to identify the differentially expressed genes associated with possible mechanisms of synergistic effect. We also performed Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analyses to determine the most enriched terms and pathways of upregulated and downregulated genes. We found 460 genes in which iBET-151 and paclitaxel combination treatment changed more than single-treatment or no-treatment. Thus, additional functional studies are needed, but our results provide the first evidence of the synergistic effect between iBET-151 and paclitaxel in regulating the transcriptome of GC cells.

• 감성과학
• /
• 제12권4호
• /
• pp.545-556
• /
• 2009

본 연구의 목적은 체크패턴의 폭과 색채조합에 따른 넥타이의 감성이미지를 알아보는 데 있었다. 본 연구에 사용된 실험도구는 체크문양의 폭(소: 0.2cm, 중: 1cm, 대: 2cm), 색조조합(유사: 하양+밝은, 반대: 하양+어두운), 색상조합(WR: 하양+빨강, WB: 하양+파랑, WG: 하양+회색)으로 만든 총 18개의 사진 자극물과 체크넥타이의 감성이미지를 평가하기 위해 의미미분척도를 사용하였다. 피험자는 경남에 거주하는 여대생 216명을 대상으로, 조사는 2009년 9월에 실시되었다. 자료분석은 SPSS프로그램을 사용하여 변량분석과 Duncan-test를 실시하였다. 그 연구결과는 다음과 같다. 체크패턴의 폭과 색채조합에 따른 넥타이의 감성이미지를 요인분석한결과 매력성, 젊음, 현시성, 품위성, 온유성의 5가지 차원으로 도출되었다. 폭은 현시성 차원에서, 색조조합은 매력성, 현시성, 품위성, 온유성 차원에서 독립적인 영향을 미쳤으며, 이 두 변인 간에는 서로 상호작용하여 매력성 차원에 유의적인 영향을 미쳤다. 색상조합은 감성이미지의 모든 차원에서 독립적인 영향을 나타내었다. 또한 색상조합은 폭과 상호작용하여 매력성, 젊음, 현시성, 품위성 차원에 유의적인 영향을 미쳤고, 색조조합과 상호작용하여서도 매력성, 젊음, 현시성 차원에 두드러진 단서로 작용하였다.

• 약학회지
• /
• 제53권4호
• /
• pp.184-188
• /
• 2009

Fluorouracil (5-FU) is one of the most widely used chemotherapeutic drugs in the treatment of advanced colorectal cancer patients. Capsaicin (N-vanillyl-8-methyl-alpha-nonenamide), a spicy component of hot pepper, is a homovanillic acid derivative that preferentially induces cancer cells to undergo apoptosis. The purpose of the present study is to examine whether capsaicin enhances the anticancer effect of 5-fluorouracil in HT-29 human colon cancer cells by inducing apoptosis, and whether PPARgamma is involved in the capsaicin action in combination treatment with 5-FU. Treatment of the cells with either 5-FU or capsaicin alone for 48 h had little effect on the cell viability up to $50{\mu}M$ concentration, whereas co-treatment of the cells with capsaicin in the presence of 5-FU for 48 h significantly decreased the cell viability in a concentration-dependent manner. In addition, caspase-3 activity, a marker enzyme for apoptosis, was significantly increased by the combined treatment with 5-FU and capsaicin compared to the 5-FU or capsaicin alone treatment. Also, treatment with troglitazone, a peroxisome proliferator-activated receptor gamma ($PPAR{\gamma}$) agonist, further enhanced the effect of the combination treatment on the cell viability and caspase-3 activity, and bisphenol A diglycidyl ether (BADGE), a $PPAR{\gamma}$ antagonist, blocked the effect of the combination treatment. These results suggest that the combination treatment of HT-29 cells with 5-FU and capsaicin induces apoptotic cell death at relatively low concentration than each drug alone, and the combination treatment may be associated with the $PPAR{\gamma}$ pathway activation.

• 대한한의학방제학회지
• /
• 제27권1호
• /
• pp.53-63
• /
• 2019

Objectives : In this study, we investigated the combination effects of Cinnamomi cortex Ethanol Extract (CcEE) and hyperthermia in the human AGS gastric cancer cell line. Methods : AGS cells were treated with the indicated concentrations of CcEE (0, 50 or $60{\mu}g/mL$) for 1h prior to hyperthermia. And then incubated for a further 30 min at the indicated temperatures (37, 42 or $43^{\circ}C$) in a humidified incubator containing 5% $CO_2$ or a thermostatically controlled water bath for hyperthermia. The cell viability was measured by MTT assay, Morphology assay and Trypan blue assay. To investigate the possible molecular signaling pathways, the activation of mitogen-activated protein kinase (MAPK) proteins (ERK, p38 and JNK) and expression of various anti-apoptotic proteins such as Caspase-3, Caspase-9, p53, Cyclin D1 and MMP-2 were assessed by Western blot analysis. In addition, Annexin V and 7-amino-actinomycin D (7-AAD) staining was performed to examine the apoptotic mechanism. Results : Combination of CcEE with hyperthermia effectively suppressed the cell viability and changed cellmorphology compared with CcEE or hyperthermia treatment alone. Combined treatment also abated the expression of Caspase-3, Caspase-9, Cyclin D1 and MMP-2. Whereas, the expression level of p53 was up-regulated by co-treatment. Moreover, combination treatment enhanced phosphorylation of ERK, p38 and JNK. In addition, this combination increased anti-cancer effect by inducing cell death through the apoptosis. Conclusions : Taken together, all these findings suggest that the combination treatment with CcEE and hyperthermia may have therapeutic potential as a promising approach to patients with stomach cancer.

• 동의생리병리학회지
• /
• 제26권4호
• /
• pp.432-436
• /
• 2012

Among a number of factors related to the effect of acupuncture and moxibustion treatment, prescriptions do important work. Traditional eastern doctors also placed emphasis on prescriptions of acupuncture and moxibustion, and a lot of experiences have been accumulated. The method forms prescriptions of Sa-am acupuncture therapy pivots on five transport points. Studies show prescriptions are made on the basis of the theory of 'Wang Xiang Xiu Qiu Si'(旺相休囚死) used engendering and restraining of five phases, and it is considered the principle of transformed prescriptions through this theory. Because there is still no study analyzing prescriptions of Sa-am acupuncture by existing principles, we report opinion about Combination principle of points in them. 1. We studied history of acupuncture and moxibustion, development and kinds of points' Combination principle. 2. Around Combination principles of points in Classic of Difficult Issues and Internal Classic, we analyzed and compared between Combination principles of points in Sa-am acupuncture therapy and them in Korea and overall acupuncture methods. 1. There were many Combination principles of acupunture point combination in Classic of Difficult Issues, Internal Classic and Korea acupuncture methods. For example, Point combination of the same meridian, Point combination of the different meridian, Exterior-Interior point combination, Point combination of the same name meridian, Point combination of the multiple meridian, Point combination of Adjacent and Distant points, Left-Right point combination, Anterior-Posterior point combination, Superior-Inferior point combination, Brook point and Stream point combination, Triple energizer point combination, Eight meeting point combination, Five transport point combination, Source-Connecting point combination, Alram point and Transport point combination, Mother-Sun meridian combination, Empirical point combination, Important point combination, Point combination according to syndrome. 2. Existing Combination principles of points in prescriptions of Sa-am acupuncture therapy included every rule. 3. They could be evidences that prescriptions of Sa-am acupuncture therapy are forms inheriting and developing acupuncture and moxibustion treatments.

• Biomolecules & Therapeutics
• /
• 제2권2호
• /
• pp.103-107
• /
• 1994

Cisplatin is one of the most effective antitumor agents currently available for cancer therapy. However, its clinical use has been limited by its severe side effects, especially nephrotoxicity. Therefore, brazilin, which has a radical scavenging effect, was given intraperitoneally to evaluate the effect on cisplatin nephrotoxicity in rats. Remarkable protective effects against nephrotoxicity of cisplatin were observed when brazilin was administered to rats simultaneously with cisplatin. Hepatotoxicity induced by combination treatment of cisplatin and brazilin was evaluated by measuring serum glutamic pyruvate transaminase and serum glutamic oxalate transaminase. Combination treatment did not affect the levels of sGPT and SGOT, and any combination treatment did not induce metallothionein in kidney. Brazilin which has radical scavenging effect directly reduced nephrotoxicity of wisplatin in vivo. Thus, it seems that nephrotoxicity of cisplatin was caused by free radicals. The present results Indicate that brazilin, when it is given with cisplatin, may provide protection against cisplatin nephrotoxicity in rats.

• 한국정보통신학회:학술대회논문집
• /
• 한국정보통신학회 2021년도 춘계학술대회
• /
• pp.342-343
• /
• 2021

색의 결합효과는 개인의 경험과 기호에 따라 커다란 영향을 받게 된다. 본 논문에서는 색이 가지는 결합적인 단어에 대하여 살펴보도록 한다. 그리고 색의 결합 언어 작용은 인간의 경험에 의한 상징을 동반한다. 이렇게 색의 결합효과에서 나오는 심리적 치료 효과도 살펴본다.

• Biomolecules & Therapeutics
• /
• 제2권1호
• /
• pp.23-27
• /
• 1994

Wound healing and antibacterial effects of solcoseryl-micronomicin combination gel on an open wound were studied in mice. A simple model was designed for assessing the effects. Using the model, we compared the efficacy of a combined topical gel of solcoseryl and micronomicin with those gels of solcoseryl or micronomicin alone. From the results of our experiment, the wound healing effect of open wounds by treatment with the combination gel was significantly enhanced when compared with those by treatment with solcoseryl gel or micronomicin gel alone. And the antibacterial effect of the combination gel was higher than those of solcoseryl gel or micronomicin gel alone.

• 한국식품영양학회지
• /
• 제23권3호
• /
• pp.306-310
• /
• 2010

This study was designed to investigate the protective effect of the combination of fucoidan and lutein against AAPH-induced oxidative stress in THP-1 cells. The combination of fucoidan and lutein existed significant antioxidant effect on AAPH-damaged THP-1 cells by using lipid peroxidation and cellular antioxidant capacity assay. Fucoidan($1\;{\mu}g/m{\ell}$) and lutein($10\;{\mu}g/m{\ell}$) did not affect at all the viability of THP-1 cells, but protected the AAPH-damage of THP-1 cells at the same concentration. The viability of THP-1 cells was 0% with 1 mM AAPH alone, the protective effect of fucoidan($1\;{\mu}g/m{\ell}$) and lutein($10\;{\mu}g/m{\ell}$) was 37% and 36%, respectively. The combination of fucoidan($1\;{\mu}g/m{\ell}$) and lutein($10\;{\mu}g/m{\ell}$) exhibited significant inhibitory effect of lipid peroxidation using TBARS assay and cellular antioxidant capacity using DCFH-DA assay. In lipid peroxidation, the TBARS value of 1 mM AAPH alone was $0.8{\pm}0.03\;nM$ MDA, its of the combination of fucoidan($1\;{\mu}g/m{\ell}$) and lutein($10\;{\mu}g/m{\ell}$) was $0.2{\pm}0.05\;nM$ MDA. In cellular antioxidant capacity, the combination of fucoidan($1\;{\mu}g/m{\ell}$) and lutein($10\;{\mu}g/m{\ell}$) exhibited significant cellular antioxidant capacity of 76%, whereas quercetin($10\;{\mu}M$) as positive control exhibited the cellular antioxidant capacity of 32%. These results indicate that the cotreatment of fucoidan and lutein protects against AAPH-induced THP-1 cell damage by inhibiting lipid peroxidation, increasing cellular antioxidant capacity.

• 생약학회지
• /
• 제22권1호
• /
• pp.45-50
• /
• 1991

The combined effect of berberine isolated from the bark of Phellodendron amurense and some antibiotics was evaluated in order to measure the antibiotic activities. In this study, in the presence of streptomycin, tetracycline, cephradine as antibiotics, Staphylococcus aureus, Escherichia coli, Shigella sonnei as microorganisms were grown in an Automated Microbiology System. In case of Staphylococus aureus, combination with berberine and cephardine resulted in the strongest synergistic activity and in case of Escherichia coli and Shigella sonnei, combination with berberine and streptomycin resulted in the strongest synergistic activity. The combination with berberine and antibiotics increased the antibiotic activities, thereby showing a synergistic action.