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Determination of levels of nitric oxide in smoker and nonsmoker patients with chronic periodontitis

  • Wadhwa, Deepti;Bey, Afshan;Hasija, Mukesh;Moin, Shagufta;Kumar, Arun;Aman, Shazia;Sharma, Vivek Kumar
    • Journal of Periodontal and Implant Science
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    • v.43 no.5
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    • pp.215-220
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    • 2013
  • Purpose: Cigarette smoking is a major risk factor in periodontal diseases. The pathogenesis of periodontal diseases may be affected by alterations of the inflammatory response by smoke. Nitric oxide (NO) is a gaseous, colorless, highly reactive, short-lived free radical with a pivotal role in the regulation of various physiological and pathological mechanisms in the body. It is important in host defense and homeostasis, on the one hand, whereas, on the other hand, it modulates the inflammatory response in periodontitis, leading to harmful effects. The aim of this study was to assess the levels of NO in both the serum and saliva of smokers and nonsmokers having chronic periodontitis and to compare them with periodontally healthy controls. Methods: Sixty subjects participated in the study and were divided into three groups: group I, healthy nonsmoking subjects; group II, nonsmoking patients with chronic periodontitis; group III, smoking patients with chronic periodontitis. Each group consisted of twenty subjects. The biochemical estimation of NO in the collected serum and in the saliva was performed using the Griess colorimetric reaction. Results: The results showed that the mean value of the salivary and serum NO was greater in group II than in group I, and also greater in group III than in group II. Conclusions: NO appears to play an important and rather complex role in the immuno-inflammatory process and in the remodeling and maintenance of osseous structures. It is therefore logical that modulation of this mediator has potential for the treatment of a number of inflammatory conditions including periodontal disease.

First Record of Two Urostyloid Ciliates (Spirotrichea: Urostylida: Urostyloidea) from Brackish Water in Korea

  • Choi, Jung-Min;Kim, Ji-Hye;Shin, Mann-Kyoon
    • Animal Systematics, Evolution and Diversity
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    • v.27 no.3
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    • pp.228-238
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    • 2011
  • Two urostyloid ciliates, collected from brackish water in Korea, were identified as Diaxonella pseudorubra pseudorubra (Kaltenbach, 1960) Berger, 2006 and Pseudokeronopsis flava (Cohn, 1866) Wirnsberger, Larsen and Uhlig, 1987. The description was based on living, protargol impregnated specimens. These species are described as follows: Diaxonella pseudorubra pseudorubra: body size in vivo $145-230{\times}40-60\;{\mu}m$, elongated ellipsoidal in shape. Cytoplasm reddish and flexible. Adoral zone of membranelles occupied 30-40% of the body; composed of 33-44 membranelles; 1-3 frontoterminal cirri, 1-4 frontal row cirri, 4-6 buccal cirri, 6-10 transverse cirri. Midventral rows composed of 14-24 cirri, four left marginal rows, one right marginal row. Two kinds of cortical granules; the larger one is yellowish and the smaller one is reddish. Pseudokeronopsis flava: body size in vivo $150-210{\times}30-45\;{\mu}m$, elongated ellipsoidal shape. Cytoplasm yellowish and flexible. Adoral zone of membranelles occupied 25-30% of body; composed of 44-58 membranelles in number. Frontal cirri forming bicorona composed of 5-7 cirral pairs, 2-3 frontoterminal cirri, one buccal cirrus, and 2-3 transverse cirri. Midventral rows composed of 18-33 cirri, 34-53 left marginal cirri, and 40-58 right marginal cirri. Two kinds of cortical granules; the larger one is colorless and "blood-cell-shaped," and the smaller one is yellowish. Diaxonella pseudorubra pseudorubra is different from the most similar subspecies, D. pseudorubra pulchra, in cytoplasmic color and number of midventral cirri. Pseudokeronopsis flava is different from its most similar congeners in pigment granular color, number of bicorona, number of midventral cirri, and position of the contractile vacuole.

전자 빔 조사에 따른 토파즈의 컬러 센터에 미치는 영향

  • Sin, So-Ra;Seo, Jin-Gyo;An, Yong-Gil;Yun, Don-Gyu;Park, Jong-Wan
    • Proceedings of the Korean Vacuum Society Conference
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    • 2012.08a
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    • pp.237-237
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    • 2012
  • 토파즈는 Al2SiO4 ((F,OH)2) 화학성분을 이루고 있는 광물로써 주로 Fe, Cr, V 등과 같은 불순물을 포함하고 있다. 토파즈는 colorless, blue, pink, yellow 등 다양한 컬러로 산출된다. 결정 내 Si4+ 가 있어야 할 자리에 Al3+ 가 치환되면 Al3+ 를 둘러싸고 있는 4개의 산소 중 하나의 최외각 전자가 전자가를 맞추기 위해 주변에 있던 수소와 결합하여 hole center를 형성한다. 이때 토파즈는 옅은 황색의 컬러를 발색하게 된다. 그 외 청색과 핑크색 등의 컬러는 Al3+ 자리에 치환된 Fe, Cr 등과 같은 불순물에 의해 발색을 일으킨다. 또한 토파즈는 인위적으로 감마선, 전자 빔, 양성자 빔, 중성자 빔을 통해서도 hole center를 형성시켜 컬러를 발색시킬 수 있다고 잘 알려져 있다. 본 연구에서는 총 8개의 무색 토파즈를 이용하여 다양한 조건(energy 및 dose)의 전자 빔 조사를 통해 각 조건 별 컬러변화 및 분광학적 특성변화를 관찰하였다. 모든 시료는 WD-XRF를 통해 정성분석을 하였고, 전자스핀공명(ESR)기기를 통해 전자 빔 조사 전과 후 전자의 스핀 특성 변화를 관찰하였다. 자외선-가시광선 분광분석결과 모든 시료는 전자빔 조사 후 황색과 관련이 있는 450 nm 부근의 파장 영역에서 흡수 peak가 증가하는 것을 확인할 수 있었다. 또한 전자 빔 조사 후 전자스핀공명 분석 결과에서도 Fe3+ 와 관련이 있는 g=3.5~4 영역은 감소하는 반면에 hole center와 관련이 있는 g=2.012 영역이 증가하는 향상을 보였다. 본 연구결과를 통해 우리는 전자 빔 조사 조건에 따라 토파즈의 결정 내부에 미치는 영향 및 컬러 변화와의 상관관계에 대해 확인할 수 있었다.

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Coordination Modes and Properties of Ag(I) Complex with N,N,N',N',N''-Pentamethyldiethylenetriamine

  • Chun, In-Sung;Kwon, Jung-Ah;Bae, Myung-Nam;Lee, Sim-Seong;Jung, Ok-Sang
    • Bulletin of the Korean Chemical Society
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    • v.27 no.7
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    • pp.1005-1008
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    • 2006
  • The reaction of $AgClO_{4}$ with acyclic potential tridentate N,N,N',N',N''-pentamethyldiethylenetriamine (pmdeta) has given colorless crystals suitable for X-ray crystallography. The crystal structure ($P2_{1}$/n, a = 14.413(1) $\AA$, b = 25.270(2) $\AA$, c = 16.130(1) $\AA$, b = $103.012(1){^{\circ}}$, V = 5723.7(8) A$\AA^{3}$, Z = 4, R = 0.0349) has been solved and refined. Three silver(I) ions connect four pmdeta ligands to produce discrete complex of $[Ag_3(pmdeta)_4](ClO_4)_3$. A pmdeta ligand is bridged to three silver(I) ions, and three other pmdeta ligands are chelated to each silver(I) center in a tridentate mode. Thus, the product is a rare tri-nuclear silver(I) complex with two different chemical environments. $^{13}C$ NMR and $MAS\;^{13}$C NMR indicate that the tri-nuclear silver(I) complex is not rigid in solution. The contact angles and thermal analyses of the complex are measured and discussed.

Studies on the Constituents of Spiraea Koreana Nakai (참조팝나무의 成分 Alkaloid 에 關한 硏究)

  • Jin, Kab-Dukc
    • Journal of the Korean Chemical Society
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    • v.11 no.3
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    • pp.111-116
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    • 1967
  • A new alkaloid named Spirajine(m.p. 182~$184^{\circ}C$ $[{\alpha}]d^{19}+3.4^{\circ}$ in $CHCl_3$, $C_{23}H_{33}NO_3$, colorless prism) was isolated from the leaves of Spiraea Koreana Nakai (Spiraeceae) (Korean name "Chamjopab namu") which grows in the mountaineous area of Korea, by process of Scheme I (yields 0.13%). Another two unidentified alkaloids (not yet crystallized) were separated by the method of thin layer chromatography. (The Rf values of the two unidentified alkaloids were 0.66, 0.77, respectively and Spirajine 0.72) Spirajine were subjected to the structural investigation with the use of ultra violet and infra red spectrophotometry, and opical rotatory dispersion. The alkaloid contains two ketonic carbonyl groups, tertiary hydroxyl group, methyl groups, N-methyl group and both cyclohexane ring and cyclopentane ring.

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Histological Study on Cutaneous Albinism of Korean Slender Gudgeon Squalidus gracilis majimae from Korea (긴몰개 Squalidus gracilis majimae의 피부백색증에 대한 조직학적 연구)

  • Lee, Yong-Joo;Park, Jong-Young;Oh, Min-Ki
    • Korean Journal of Ichthyology
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    • v.21 no.3
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    • pp.208-213
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    • 2009
  • Histological examination of an individual of the Korean slender gudgeon Squalidus gracilis majimae having cutaneous albinism produced rarely in the wild was made and compared with a normal individual. The external body of the albino was colorless, differing from the normal individual, which has dense brownish black spots over its body surface. To make it clear through histological study, we observed eight skin regions: dorsal, lateral, ventral, upper caudal peduncle, lower caudal peduncle, dorsal fin, anal fin, and the eyes. These regional skins were the same in fundamental structure between albinic and normal gudgeon, but there were significant differences in distribution and development of pigment cells (melanins). In the normal gudgeon, the pigment cells were well developed over the regional skins except on the skin from the ventral region. However, it was confirmed in the albino that the pigment cells were vestigial over the upper regions of the eye and body but absent in the ventral region, lower caudal peduncle, and anal fin.

Cultural Characteristics of New Cultivar of Lentinula edodes, Poongnyunko (표고 신품종 풍년고의 배양 특성)

  • Park, Young-Ae;Bak, Won-Chull;Koo, Chang-Duck;Lee, Bong-Hun
    • The Korean Journal of Mycology
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    • v.43 no.1
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    • pp.26-32
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    • 2015
  • In this study, a new cultivar of Lentinula edodes was bred from dikaryotic KFRI 490 and monokaryotic KFRI 536 by Di-mon method. The new breed was named "Poongnyunko" and physiological characteristics was investigated. Its optimal growing temperature was $25^{\circ}C$, and it had thin density. Its colony was colorless and did not form any epithelium. On the $7^{th}$ day of mycelial growth, "Poongnyunko" was 56.6 mm, and showed higher cellulase activity compared with "Sanlim 4-ho"(52.9 mm). On the $5^{th}$ day of mycelial growth, "Poongnyunko" was 58.0 mm, and showed higher laccase activity compared with "Sanlim 4-ho" (55.6 mm). Its pH at a substrate was 6.0. Before cultivation, the pH of sawdust substrate was 4.7-5.5 went down to 3.7-3.9 after 120-day cultivation. After 60 days of cultivation, the change of $CO_2$ was the greatest. During the cultivation, $CO_2$ concentration in a growing bag was 4.67% to 3.90%.

Beta glucan: Heath benefits of optimal ingredients via the mixture ratio of rice bran and Sarcodon aspratus

  • Seo, Yu-Ri;Kim, Hye-Been;Cheong, Kyu-Min;Lim, Ki-Taek
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 2017.04a
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    • pp.101-101
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    • 2017
  • Beta-glucan is a polymerized polysaccharide on beta-1,3 chemical bonds. It has the main pharmacological action of various anticancer mushrooms and is colorless and odorless. In addition, it enhances phagocytosis of macrophages against mycobacterium tuberculosism and increases resistance of host against food poisoning bacteria. Owing to these advantages, beta-glucan was used in various health supplement foods such as immune boosters, hypotensive agent and hypoglycemic agent. Our study was aimed to investigate the effective components of beta-glucan, which was optimized via the contents of Sarcodon aspratus and rice bran. First, the mixture ratio condition of Sarcodon aspratus and rice bran were respectively 10:0, 7:3, 5:5, 3:7, and 0:10. The raw materials were fabricated using hot water extraction method. Distilled water of 100 mL was added to the raw material and extracted. After the extraction processed, the content of effective components was analyzed. The absorbance of beta-glucan was measured using a beta-glucan kit (Megazyme, (1-3) (1-4) BETA-D-GLUCAN ASSAY KIT). The absorbance analysis was repeated three times for accurate analysis. After the extracts were lyophilized, the yields of extracted raw materials according to the mixture ratio conditions of Sarcodon aspratus and rice bran (10:0, 7:3, 5:5, 3:7, and 0:10) were respectively 33.1%, 34.5%, 35.3%, 30.7%, and 24.3%. The absorbance was 1.52, 1.47, 1.50, 1.79, and 1.56, respectively. As a result, the optimum ratio of beta-glucan is 3:7 at Sarcodon aspratus and rice bran. This study suggested that the optimal amount of beta-glucan could be used as a health supplement foods and food additive such as immune boosters, hypotensive agent and hypoglycemic agent. However, the new condition (temperature, time) of hot water extraction to maximize the content of beta-glucan could be considered, could be necessary to compare with the existing extracts.

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A Case of Acute Lung Injury Caused by Tetrafluoroethylene Inhalation (Tetrafluoroethylene 흡입에 의한 급성폐손상 1예)

  • Lee, Su Ok;Choi, Eun Jung;Kim, Ka Young;Kim, Jun Chul;Park, Jung Chul;Jung, Chi Young;Kim, Yeon Jae;Lee, Byung Ki
    • Tuberculosis and Respiratory Diseases
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    • v.62 no.3
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    • pp.223-226
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    • 2007
  • Tetrafluoroethylene is a colorless gas that can be used to synthesize a variety of fluoride compounds by polymerization (e.g., Teflon). Fluoride compounds have many applications in industry. There are several reports of inhalation injury from the pyrolytic product of fluoride compounds. When the polymer is heated under the conditions of inadequate ventilation, the fumes can cause polymer fume fever or pulmonary edema which manifested as symptoms such as fever, chill, profuse sweating, cough and dyspnea. However there are no reports of a direct lung injury caused by tetrafluoroethylene. We report a case of a 27-year-old male presented with acute lung injury after inhaling concentrated tetrafluoroethylene. He complained of cough and dyspnea after the accidental inhalation of tetrfluoroethylene at his workplace. The symptoms improved without any complications after conservative treatment with oxygen and steroid.

Dissolution Conditions of Solid Radioactive Wastes Generated from NPP for the Analysis of Radionuclides Using a Closed-vessel Microwave Acid Digestion System (원전 발생 고체 방사성폐기물 내 핵종 분석을 위한 극초단파 산분해 장치를 이용한 용액화 조건)

  • 표형열;이정진;전종선;이창헌;지광용
    • Proceedings of the Korean Radioactive Waste Society Conference
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    • 2004.06a
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    • pp.158-166
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    • 2004
  • The optimal conditions are obtained for the decomposition of solid radioactive wastes, including ion exchange resin, zeolite, active charcoal, and sludge from nuclear power plant. In the process of decomposing the radioactive wastes were used the microwave acid digestion method with mixed acid. The solution after acid digestion by the following method was colorless and transparent. Each solution was analyzed with ICP-AES and AAS and the recovery yield for 5 different elements added the simulated radioactive wastes were over 94%. As an effective pre-treatment, the proposed microwave acid digestion conditions concerning the chemical trait of each radioactive waste are expected to be generally applied to above-mentioned radioactive wastes from nuclear power plant hereafter.

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