• Asian Pacific Journal of Cancer Prevention
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• v.14 no.10
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• pp.6131-6134
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• 2013

Background: The two basic methods that are currently accepted to identify the HER2 status are immunohistochemistry and flyorescence in situ hybridization (FISH). The aim of this study was to perform the dual-color silver in situ hybridization (dc-SISH) technique as an alternative to FISH. Materials and Methods: A total of 40 invasive breast carcinoma cases were assessed for HER2 gene amplification by FISH and dual-color SISH. Results: Significant correlation was found in the HER2 expression results obtained with the two approaches (p=0.001, p<0.05). The concordance rate was 92.3%. Conclusions: Foutine practical use of the dc-SISH method, which is much easier to apply, score, and evaluate, has many advantages. HER2 and CEN17 status can be evaluated simultaneously with the newly developed "Dual-Color Probe". All these specifications and the reliable results obtained support the widespread use of SISH technique in clinical practice.

• Journal of The Korean Society of Grassland and Forage Science
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• v.16 no.4
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• pp.253-259
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• 1996

Ammonia microdiffusion method and colorimetric measurement are described for the nitrogen determination. The diffusion of ammonia could be successfully induced by using a microdiffusion cell. It is a simple and rapid technique, which is suitable for transforming the nitrogen in digests into $NH_4CI$ for the colorimetric N determination with ammonia color reagent. Above 99% of N recovery were obtained with microdiffusion up to 15 hours. The coloration method of collected $NH_4CI$ for the colorimetric N determination was also estabilshed with a scanning in U.V. spectrophotometer. Under the proposed coloration method (0.5 mL of sample digest, 4.0 mL of $H_2O$ and 0.5 mL of ammonia color reagent), a maximal absorbance was observed at 410 nm. The kinetic measurement of absorbance showed a high stability from 5 to 45 minutes after color development. Absorbance was directly proportional to the amount of $NH_4^+-N$ present. The microdiffusion-ammonia coloration method was successfully applied to the nitrogen determination in the forms of protein-N or total -N in plant tissue. Comparing with Kjeldahl distillation method, the values obtained with described method were slightly higher and more reliable.

• Horticultural Science & Technology
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• v.33 no.4
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• pp.559-565
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• 2015

The rind phenotype of watermelon fruits is an important agronomic characteristic in the watermelon market. Inheritance and linkage analyses were performed for three rind-related traits that together determine the rind phenotype: foreground stripe pattern, rind background color, and depth of rind color. The inheritance of the foreground stripe pattern was analyzed using three different $F_2$ populations, showing that the striped pattern is dominant over the non-striped pattern. The inheritance analysis of the rind background color was performed using $F_2$ populations of the '10909' and '109905', and the depth of rind color was analyzed using $F_2$ populations of the '90509' and '109905'. Yellow color was found to be dominant over green color, and a deep color was dominant over the standard color. Linkage analysis of the three traits was conducted using three $F_2$ populations in which two traits were segregating. Each pair of traits was inherited independently, which demonstrated that the three traits are not linked. Therefore, we propose a three-locus model for the determination of rind phenotype, providing novel insight that rind phenotype is determined by the combination of three genetically independent loci.

• Journal of the Korean Geotechnical Society
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• v.38 no.5
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• pp.61-71
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• 2022

Soil color is used to determine soil classification and its physical, chemical, and biological properties. Visual determination is the most commonly used method for identifying soil color. However, it is subjective and, in many cases, non-repeatable. Digital image processing obtains useful information from digital images, accelerates soil classification, and enables the rapid identification of soil types in a field. This study develops a digital image processing-based soil color analysis technology that can consider irregular light conditions in the field. The digital image studio was designed to simulate the characteristics of natural light (illuminance and color temperature). Also, digital images of two soil samples (Jumoonjin sand and Anseong weathered soil) were captured under 12 different light conditions. For the RGB and CIELAB color systems, soil color intensities of 24 images were obtained using digital image processing. CIELAB was suitable for dealing with irregular light conditions in the field.

• Mycobiology
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• v.32 no.1
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• pp.6-10
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• 2004

White and brown strains of Flammulina velutipes were inter-crossed. All $F_1$ showed light-brown fruitbody, suggesting that a gene for the brown fruitbody was incompletely dominant against the white one. And backcross experiment showed that more than two genes were involved in color determination. To isolate a molecular marker linked to fruitbody color, a set of primers was designed from a sequence of clones derived by a bulked segregant analysis. These markers showed a specific band which co-segregated with brown fruitbody forming strains.

• Journal of Environmental Health Sciences
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• v.1 no.1
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• pp.32-35
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• 1974

Acidic substances contained in asphalt are affect upon emulsitiability of asphalt and its adhesion with aggregates, and therfore, detemination of acidity of asphalt is of important one. However, there has not been established a method of precisely determining the acidity of asphalt. This paper reports on an improved color-indicator titration method, by which the acid number of asphalt is determined. By treating benzene solution of asphalt with alcohol, acidic constituents were selectively extracted into alcohol solution, while asphaltic matters precipitated. A color-indicator titration method then was applied to the resultant faint-colored solution. The change of color at end point was clearly observed, and the results were obtained with good reproductivity and checked well with the results by the I.P. 177/64 method. Alcohols having not more than three cabon atoms have been found suitable for extraction.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.2 no.2
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• pp.9-14
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• 2009

This study was conducted to develop a electronic circuit of primary color reaction for urine analyzer for measuring color response of urine strip. A primary color reaction system is equipped with the computer, electronic circuit, tray, detecting assembly and software. The determination of coefficient($R^2$) between reagent and color sensor were 0.9801(R), 0.9868(G) and 0.9837(B). To evaluate the system verification, we measured the primary color reaction of erythrocytes, Bilirubin, Urobilinogen, Ketones and Protein. We concluded that it is possible to use the developed the primary color reaction system for urine analyzer using u-health.

• Korean Journal of Remote Sensing
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• v.21 no.3
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• pp.173-188
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• 2005

The present study aims to derive and compare narrow and broad bandwidths of ocean color sensor’s algorithms for the study of monitoring highly dynamic coastal oceanic environmental parameters using high-resolution imagery acquired from Multi-spectral Camera (MSC) on KOMPSAT-2. These algorithms are derived based on a large data set of remote sensing reflectances ($R_{rs}$) generated by using numerical model that relates $b_b/(a + b_b)$ to $R_{rs}$ as functions of inherent optical properties, such as absorption and backscattering coefficients of six water components including water, phytoplankton (chl), dissolved organic matter (DOM), suspended sediment (SS) concentration, heterotropic organism (he) and an unknown component, possibly represented by bubbles or other particulates unrelated to the first five components. The modeled $R_{rs}$ spectra appear to be consistent with in-situ spectra collected from Korean waters. As Kompsat-2 MSC has similar spectral characteristics with Landsat-5 Thematic Mapper (TM), the model generated $R_{rs}$ values at 2 ㎚ interval are converted to the equivalent remote sensing reflectances at MSC and TM bands. The empirical relationships between the spectral ratios of modeled $R_{rs}$ and chlorophyll concentrations are established in order to derive algorithms for both TM and MSC. Similarly, algorithms are obtained by relating a single band reflectance (band 2) to the suspended sediment concentrations. These algorithms derived by taking into account the narrow and broad spectral bandwidths are compared and assessed. Findings suggest that there was less difference between the broad and narrow band relationships, and the determination coefficient $(r^2)$ for log-transformed data [ N = 500] was interestingly found to be $(r^2)$ = 0.90 for both TM and MSC. Similarly, the determination coefficient for log-transformed data [ N = 500] was 0.93 and 0.92 for TM and MSC respectively. The algorithms presented here are expected to make significant contribution to the enhanced understanding of coastal oceanic environmental parameters using Multi-spectral Camera.

• Environmental Engineering Research
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• v.12 no.5
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• pp.203-210
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• 2007

Since the emissions composition from the household products have potentially been associated with health risks for building occupants, the chemical composition emitted from the products should be surveyed. The current study identified the emission composition for 42 liquid household products, using a purge-and-trap method. This evaluation was done by classifying the household products into five product classes (deodorizers, household cleaners, color removers, pesticides, and polishes). Nineteen compounds were chosen on the basis of selection criteria. The quality control program for purge-and-trap and analytical systems included tests of laboratory blank Tenax traps and blank water samples, and the determination of calibration equation, measurement precision, method detection limit (MDL), and recovery. The number of chemicals varied according to the product categories, ranging from 4 for the product category of bleaches to 12 for the product categories of air fresheners and nail color removers. For all product categories, the emission composition and concentrations varied broadly according to product. It is noteworthy that most household products emit limonene: 19 of 25 cleaning products; 5 of 6 deodorizers; 1 of 3 pesticides; 3 of 3 color removers; and 4 of 5 polishes. It was suggested that the use of household products sold in Korea could elevate the formation of secondary toxic pollutants in indoor environments, by the reaction of limonene with ozone, which entered indoor environments or might be generated by indoor sources such as electronic air cleaning devices and copying machines.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.6
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• pp.858-861
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• 2001

Near Infrared spectroscopy was applied to the samples of processed pork to see the effect of grinding on chemical components analyses. Data from conventional chemical analyses of moisture, fat, protein, NaCl were put into calibration model by NIR of reflectance mode. The other properties observed were pH and color parameters ($L^*,\;a^*,\;b^*$). Spectral ranges of 400~2500 nm and 400~1100 nm were compared for color parameters. Spectral ranges of 400~2500 nm and 1100~2500 nm were compared for chemical components and pH. Different spectral ranges caused little changes in the coefficients of determination or standard errors. $R^{2,}s$ of calibration models for color parameters were in the range of 0.97 to 1.00. $R^{2,}s$ of calibration models of intact sausages for moisture, protein, fat, NaCl and pH were 0.98, 0.89, 0.95, 0.73 and 0.77, respectively using spectra at 1100~2500 nm. $R^{2,}s$ of calibration models of ground sausages for moisture, protein, fat, NaCl and pH were 0.97, 0.91, 0.97, 0.42 and 0.56, respectively using spectra at 1100~2500 nm.