• 대한간호학회지
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• 제12권1호
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• pp.39-52
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• 1982

Nosocomial infection is important in the management of the nursing care and has been found to be related with the airborne microbes contamination. The purpose of this study is to identify the differences of the airborne microbes between hospitals, nursing units, days and times and to identify the difference in the types of microorganisms between hospitals, nursing units, days and times. This study was conducted from May 25, to July 10, 1981, according to R. Koch's methods for quantative samplings of airborne microbes and the results of this study were reviewed in a statistical method. The following conclusions were obtained: 1. There was a significant difference in the types of airborne microbes between 8 hospitals (F=5.0491, p<0.01) 2. There was a significant difference in the types of airborne microbes between surgical, medical, nursery, 1.C.U., operating theatre and outpatient nursing unit. (F=2.1764, p<0.05) 3. There was not a significant difference in the types of airborne microbes between Monday, Thursday and Saturday (F= 1.6365, p>0.05) 4. There was a significant difference in the types of airborne microbes between AM 7 : 00, AM 11：00 and PM 3 : 00. (F=7.2951, p<0.01) 5. 4061 colonies were divided into more 48 types and the classification was as follows. gram positive cocci (2024 colonies) 49.3% gram positive bacillus (1211 colonies) 29.8% gram negative bacillus (577 colonies) 14.2% fungus(200colonies) 4.9% gram negative cocci (41 colonies) 1.0% other's(8 colonies) 0.3% 6. There was a significant difference of airborne microbes between 8 hospitals. (F=7.7943, P<0.01) 7. There was not a significant difference of airborne microbes between 6 nursing units. (F= 1.8461, P>0.05) outpatient nursing unit : 12.6 colonies surgical nursing unit. 10.4 colonies Medical nursing unit : 9.8 colonies nursery nursing unit : 9.4 colonies operating theatre nursing unit：8.2 colonies Intensive care nursing unit : 7.8 colonies 8. There was not a significant difference of airborne microbes between Monday, Thursday and Saturday. (F=1.4371, p>0.05). Saturday : 11.0 colonies Monday : 9.1 colonies Thursday : 9.1 colonies 9. There was a significant difference of airborne microbes between A. M. 7 ; 00, A. M. 11 : 00 and P .M. 3：00 (F=7.6658, p<0.05) P. M. 3 : 00 : 12.2 colonies A. M. 11 : 00 : 10.0 colonies A. M. 7 : 00 : 7.1 colonies.

• 대한수의학회지
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• 제15권2호
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• pp.279-285
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• 1975

The experiments were conducted to isolate the etiogical agent and to survey the distribution of Nosema disease in honey bees. The results obtained were as follows: 1. The etiological agent of the so-called "crawling disease" in honey bees characterized by the symptoms of crawling, diarrhea, and enteritis etc. was first isolated and identified with Nosema apis (Zander 1909) in Korea. 2. 455 colonies were randomely sampled and surveyed in 4,766 bee colonies out of 56 apiaries and 51 colonies (11.2%) out of 455 bee colonies were infected with N, apis. 3. Infection rates according to the period of honeyflow as follows: Brassica napus (Apr.): 25/130 colonies (18.4%) Rohinia pseudoacacia (May) : 8/55 colonies (14.%) Trifolium repels(Jun.): 15/99 colonies (13.6%) Castanea crenate (Jul.): 3/46 colonies (6.5%) Lespedeza bicolor(Aug.): 0/60 colonies (-) Fagopyrumesculentum(Sept.) & Perilla frutescens(Oct.) 0/65 colonies (-) 4. The typical clinical signs of Nosema disease were appeared on loth day after N. apis was orally administered with the level of $16{\times}10^4$ spores/ml to the healthy adult bees. Spores could be harvested with the level of $121{\sim}236{\times}10^4$ spores/ml on 10th day and $392{\sim}429{\times}10^4$ spores/ml on 15 days after infection. 5. In adult honey bees infected with N. apis artificially the 50% lethal day of life-span was 9 to 10 days and 100% lethal day was 16 to 19 days. However, in the control 50% lethal day was 19 to 23 days and 100% lethal day was 31 to 33 days.

• 대한미생물학회지
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• 제5권1호
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• pp.19-26
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• 1970

1. Of the 35 various grains, 446 colonies of fungi were isolated. Among the 446 colonies, 423 were possible to identify into 17 genera. 2. Of the 10 bean-pastes, fungi were isolated 193 colonies. It can be identified 11 genera. 3. It will be noted that Aspergillus sp., Penicillium sp., Mucor sp., Cladosporium sp. were predominated genera. 4. A total of 155 colonies of Aspergillus sp. isolated from various grains were obtained from the samples. Asp. versicolor was the frequent species and 47 colonies isolated. 5. Penicillium where was isolated from various grains, obtained 115 colonies. Pen. camemberti was the most frequent species. 6. Penicillium palitans was most excellent amylase producing fungi and which was 1ml per 6.300 unit.

• Ocean and Polar Research
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• 제27권1호
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• pp.25-34
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• 2005

Impacts of Typhoon Maemi on a soft-coral community located on subtidal cliff at Munseom, Jeju were investigated in this study using underwater photography. Typhoon Maemi hit Jeju Island in late September 2003 and its impact was strong enough to destruct most shallow water sessile benthos including soft corals. To estimate numbers and size of soft-coral colonies, a line transect was installed on the cliff at depth from 3 to 9 m and photographs were taken serially by every 1m. From each $1{\times}1m$ underwater photograph, species and size of soft-coral colony was determined. Number of soft-coral colony and its Percent coverage (PC) in each $1m^2$ quadrat was calculated. Soft corals Scleronephthya gracillium, Dendronephthya gigantea, D. spinulosa and D. castanea were identified from the photographs. Dendronephthya sp. was mainly distributed at 3-6m while S. gracillimum was mostly occurred at $6{sim}9m$. A survey conducted before the typhoon showed that number of the soft-coral colonies at $3{\sim}4m,\;4{\sim}5m,\;5{\sim}6m,\;6{\sim}7m,\;7{\sim}8m\;and\;8{\sim}9m$ was 17, 24, 20, 23, 18 and 30 $colonies/m^2$ or 21, 48, 36, 28, 24 and 43%, respectively. After the typhoon, number of soft-coral colonies in the transect increased, 31, 35, 21, 10, 21 and 50 $colonies/m^2$ while PC was remarkably decreased as 21, 23, 21, 5, 9 and 13%, respectively. Our data suggested that the impact was limited in larger colonies; larger soft coral colonies were selectively destroyed and removed while the small colonies underneath the larger colonies remained undestroyed.

• Asian-Australasian Journal of Animal Sciences
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• 제13권2호
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• pp.137-148
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• 2000

This paper is based on the 9 goat colonies along the middle and lower Yellow River valley and 7 local goat colonies in the Northeast, Tibet and the Yangtze valley. After collecting the same data about the 22 goat colonies in China and other countries, it establishes and composes the matrix of fuzzy similarity relation describing the genetic similarities of different colonies. It also clusters 38 colonies according to their phylogenetic relationship. The establishment of the matrix and the cluster are effected in terms of the frequency of 18 loci and 43 allelomorphs in blood enzyme and other protein variations. The study proves that the middle Yellow River valley is one of the taming and disseminating centers of domestic goats in the South and East of Central Asia. Compared with other goat populations in this vast area, the native goat populations in the west of Mongolian Plateau, the Qinghai-Tibet Plateau and the middle Yellow River valley share the same origin. The colonies in the lower Yellow River valley and those in the middle valley, however, are relatively remote in their phylogenetic relationship. The native goat colonies in the southeast of Central Asia can be classified into two genetic groups: "East Asia" and "South Asia" and the colonies in Southeast Asia belong to either group.

• ALGAE
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• 제34권2호
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• pp.99-109
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• 2019

Habitat-forming species increase spatial complexity and alter local environmental conditions, often facilitating the assembly of plants and animals. We conducted a trait-based approach to algal assemblages associated with the freshwater bryozoan, Pectinatella magnifica. Association with algae leads to the inner bodies of the bryozoans being colored green; this is frequently observed in the large rivers of South Korea. We collected the green-colored gelatinous matrices and phytoplankton from waterbodies of the two main rivers in South Korea. Algal assemblages within the colonies and in the waterbodies were compared using the three diversity indices (richness, diversity, and dominance), and the composition of functional groups (FGs) and morphologically based functional groups (MBFGs) between the colonies within and outside of P. magnifica colonies. The most dominant and common species within the colonies were Oscillatoria kawamurae and Pseudanabaena catenata, both of which were assigned to the same FG (codon S1). Of the algal assemblages within the colonies, the dominance was higher, while the richness and diversity were lower, than those in the waterbodies. There was variation in the compositions of FGs and MBFGs in the waterbodies outside the colonies. Total nitrogen and orthophosphate led to dominance, and were significant factors for the variation in FGs in the waterbodies, whereas there were no such significant factors within the colonies. This trait-based approach to the community structure of associated algae provides the status and habitat gradient of these communities, which are stable, isolated, and consistent with the overgrowth of shade-adapted tychoplanktonic cyanobacteria.

• Current Research on Agriculture and Life Sciences
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• 제19권
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• pp.39-43
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• 2001

본 연구는 꿀벌의 월통에 얄맞은 실내 온도인 $2{\sim}9^{\circ}C$를 유지하면서 환기를 계속할 수 있는 저온양봉사에서 꿀벌의 실내월동 방법을 확립코자 수행되었다. 환기 팬의 작동 시간으로 실내 온도가 $2{\sim}9^{\circ}C$로 조절되고 환기가 행하여지는 저온양봉사에서 서양종 봉군을 공시하여 1997년부터 1999년까지 실내월동 시험을 수행한 성적은 다음과 같다. 실내월동 봉군의 폐사율은 1997~1998년 월동기간 중에 6.3~7.1% 그리고 1998~1999년 월동기간 중에 5~10%였다. 실내월동 시 월동 기간 중 봉군의 무게 감량 비율은 강군이 10.6~10.7% 그리고 약군이 10.2~11.7%였다. 월동 후 봉세 증가율은 강군의 경우는 136.1~142.3%였고, 약군의 경우는 128.0~136.5%였다.

• Preventive Nutrition and Food Science
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• 제1권2호
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• pp.168-173
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• 1996

The effect of methanol soluble fraction(MSF) of kimchi on the proliferating and differentiating activity of normal rat mammary epithelial cells or organoids in culture were studied. Reconstituted basement membrane, Matrigel, supported the growth and development several different multicellular structures from mammary organoids. The five type colonies of multicellular structures, stellate, ductal, webbed, squamous, and lobulo-ductal colonies, were observed in Matrigel culture. In methanol extract groups, webbed colonies were more and squamous colonies were less than control group. and the lobulo-ductal colonies which is known that it formed in well differentiated mammary epoithelial cells were developed more in MSF treated group than control group. These results showed that methanol extract of kimchi affected on the proliferation and differentiation of normal rat mammary epithelial cells cultured in serum free medium condition.

• Archives of Pharmacal Research
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• 제12권4호
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• pp.249-253
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• 1989

High producers or blocked mutants of aminoglycoside antibiotic-producing Streptomyces spp. were selected by application of an agar plug method and by culturing individual colonies in broth. The productivities of aminoglycoside antibiotic producing organisms were increased by selection of a high producer from colonies obtained by spreading spores of wild strain, or survived from treatment of a mutagen or from the colonies regenerated from protoplast-formation and cell-wall regenerations. Some mutagen treated colonies lost the ability to produce antibiotics (5-8%). Some A-factor negative and deostreptamine or streptidine negative mutants were obtained by N-methyl-N'-nitro-N-nitrosomethylguanidine (MNNG) treatment. Many of the survivors from the MNNG treatment lost the ability to produce antibiotics. Major colonies produced less amount of antibiotics ; only few survived colonies produced more antibiotics than the parent. Resistance of Streptomyces spp. against the antibiotics produced by itself was also markedly affected by mutagen treatment.

• 지적과 국토정보
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• 제47권1호
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• pp.161-178
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• 2017

한국 국토의 60% 이상을 차지하고 있는 산림지역에 대한 개발과 이용의 수요가 증가하고 있지만, 개발사업의 허가는 산림생태계의 보전보다는 국토 이용 중심의 평가로 이루어지고 있다. 대규모 산림지역을 평가할 경우 산림의 구조를 중심으로 분석하기 때문에 산림 내부의 변화를 관찰하고 분석하는데 한계가 있다. 따라서 본 연구에서는 충청남도 식생군락의 현존식생도와 FRAGSTATS 4.2를 이용하여 경관지수를 산출하고 식생 모자이크를 분석해 보았다. 식생 군락은 주요 자생 수종을 중심으로 대표군락과 세부군락으로 구분하였고, 군락별 차이를 분석하기 위해 분포면적(CA), 분포면적비율(PLAND), 패치개수(NP) 등의 10가지 경관지수가 선택되었다. 충청남도 식생의 대표군락과 세부군락은 소나무군락, 상수리나무군락 및 소나무와 참나무류 수종의 조합이 우세하게 나타났으며, 소나무군락의 면적이 상당히 높게 나타났다. 소나무군락과 참나무류군락의 경쟁의 경우, 대표군락의 경우 소나무군락이 전체 분포면적은 3배 가량 넓으나 작은 패치로 나뉘어져 모자이크화 정도는 참나무류 군락이 작은 것으로 판단되었다. 세부군락의 경우에는 소나무 중심의 군락에서는 소나무-신갈나무군락이, 상수리나무 중심 군락에서는 상수리나무군락이, 굴참나무 중심의 군락에서는 굴참나무-소나무군락이, 신갈나무 중심의 군락에서는 신갈나무군락이, 곰솔 중심의 군락에서는 곰솔군락이, 졸참나무 중심의 군락에서는 졸참나무-상수리나무군락이 넓은 분포면적과 동시에 모자이크화도 심한 것으로 나타났다. 전체적인 모자이크화 양상은 수치로 표현될 수 있어 모니터링 단계에서 문제가 있는 식생군락을 판별할 때 유용할 것으로 판단되었다. 모자이크화의 원인과 천이의 방향성 예측을 통한 관리 방안 마련을 위해서는 각 군락의 생태적인 특성에 대한 연구가 추가적으로 필요할 것으로 판단되었다.