• Journal of Microbiology and Biotechnology
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• v.22 no.9
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• pp.1202-1207
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• 2012

A gene, ClCDA, encoding chitin deacetylase from Colletotrichum lindemuthianum, was optimized according to the codon usage bias of Pichia pastoris and synthesized in vitro by overlap extension PCR. It was secretorily expressed in P. pastoris GS115 using the constitutive expression vector pHMB905A. The expression level reached the highest with 110 mg/l culture supernatant after 72 h of methanol induction, which comprised 77.27 U/mg chitin deacetylase activity. SDS-PAGE, mass spectrometry, and deglycosylation assays demonstrated that partial recombinant protein was glycosylated with an apparent molecular mass of 33 kDa. The amino acid sequences of recombinant proteins were confirmed by mass spectrometry.

• Research in Plant Disease
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• v.8 no.4
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• pp.224-227
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• 2002

From the necrotic stem of aquaculture Dracaena for living room decoration, we observed conidia on acervuli with occasional setae and perithecia in vivo. Morphological characters were; acervuli, dark brown and 300-500 ${\mu}{\textrm}{m}$ in diameter; conidia, hyaline, aseptate, cylindrical to slightly ellipsoid with rounded apex and narrow truncate base on phialidic conidiophores, 12.5-17.5 ${\mu}{\textrm}{m}$; setae, variable in length 1-4 septate, slightly swollen at the base and tapered to the apex, 200 ${\mu}{\textrm}{m}$; appressoria, dark brown ovate to obovate irregular 5-12.5 ${\mu}{\textrm}{m}$, perithecia on decayed stem, globose, dark brown and black 85-300 ${\mu}{\textrm}{m}$; asci 8 spored, clavate to cylindrical; 50-62.5$\times$8-10 ${\mu}{\textrm}{m}$; ascospores, oval to fusiform, sometimes slightly curved, aseptate, hyaline. Above characteristics were also confirmed in vitro. Colletotrichum state of this fungus was also compared with C. lindemuthianum and C. musae in terms of morphology of conidia and setae and growth on PDA etc. This fungus was identified as Glomerella cingulata(Stonem.) Spaulding & Schrenk(Colletotrichum gloeosporioides(Penz. Sacc.).