• Journal of Microbiology and Biotechnology
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• v.21 no.3
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• pp.263-266
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• 2011

Methylelaiophylin isolated from Streptomyces melanosporofaciens was selected as an ${\alpha}$-glucosidase inhibitor with an $IC_{50}$ value of 10 ${\mu}M$. It showed mixed-type inhibition of ${\alpha}$-glucosidase with a $K_i$ value of 5.94 ${\mu}M$. In addition, methylelaiophylin inhibited the intracellular trafficking of hemagglutinin-neuramidase (HN), a glycoprotein of Newcastle disease virus (NDV), in baby hamster kidney (BHK) cells. Methylelaiophylin inhibited the cell surface expression of NDV-HN glycoprotein without significantly affecting HN glycoprotein synthesis in NDV-infected BHK cells.

• Proceedings of the Korean Society of Toxicology Conference
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• 2005.05a
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• pp.134-134
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• 2005

• Proceedings of the PSK Conference
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• 2002.04a
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• pp.162.1-162.1
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• 2002

• Proceedings of the PSK Conference
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• 2002.04a
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• pp.202.1-202.1
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• 2002

• Journal of Microbiology and Biotechnology
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• v.24 no.7
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• pp.914-920
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• 2014

Triple-negative breast cancer (TNBC) possesses a higher rate of distant recurrence and a poorer prognosis than other breast cancer subtypes. Interestingly, most of the heat shock protein 90 (Hsp90) client proteins are oncoproteins, and some are closely related to unfavorable factors of TNBC patients. 17-Demethoxy-reblastatin (17-DR), a novel non-benzoquinone-type geldanamycin analog, exhibited potent Hsp90 ATPase inhibition activity. In this study, the anticancer effects of 17-DR on TNBC MDA-MB-231 cells were investigated. These results showed that 17-DR inhibited cell proliferation, induced apoptosis, and suppressed cell invasion and migration in the MDA-MB-231 cells. Down-regulation of the key Hsp90-dependent tumor-driving molecules, such as RIP1 and MMP-9, by 17-DR may be related to these effects. Taken together, our results suggest that 17-DR has potential as a therapeutic agent for the treatment of TNBC.

• Korean Medical Education Review
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• v.17 no.2
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• pp.88-93
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• 2015

The purpose of this study was to examine the variation in professors' satisfaction with faculty development programs and in their teaching efficacy according to their demographic characteristics and the rate of participation in faculty development programs in a medical school. The data were collected from 59 faculty members who participated in the Professor's Seminar. The questionnaire consisted of three parts: general background of the respondent; satisfaction with the faculty development program; and teaching efficacy. The data were analyzed by methods of descriptive analysis, analysis of variance, and the Mann-Whitney U-test. The program satisfaction of faculty members was significantly different by the years of educational career but it was not different by job status, specialty, gender, and the participation level in faculty programs. The faculty members' teaching efficacy differed significantly by gender and the participation level in faculty programs, while it did not differ by educational career, job status, or specialty. The results of this study suggest that various faculty programs should be developed to increase the satisfaction level of different groups of faculty members, and that they should be more focused on teaching efficacy, as it is considered to be one of the most effective way to increase the quality of education.

• Proceedings of the Korean Magnestics Society Conference
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• 2007.05a
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• pp.170.1-170.1
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• 2007

• Proceedings of the Korean Magnestics Society Conference
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• 2007.05a
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• pp.154.2-154.2
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• 2007

• 대한예방의학회:학술대회논문집
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• 2002.10a
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• pp.65-66
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• 2002

• Journal of Microbiology and Biotechnology
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• v.26 no.1
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• pp.56-60
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• 2016

Two new glucosides (1 and 2) of geldanamycin (GA) analogs were obtained from in vitro glycosylation by UDP-glycosyltransferase (YjiC). Based on spectroscopic (HR-ESI-MS, 1D, and 2D-NMR) analyses, the glucosides were elucidated as 4,5-dihydro-7-O-descarbamoyl-7-hydroxyl GA-7-O-β-D-glucoside (1) and ACDL3172-18-O-β-D-glucoside (2). Furthermore, the water solubility of compounds 1 and 2 was about 215.2 and 90.7 times higher respectively, than that of the substrates. Among compounds 1-4, only 3 showed weak antiproliferative activity against four human tumor cell lines: MDA-MB-231, SMMC7721, HepG2, and SW480 (IC₅₀: 13.6, 15.1, 31.8, and 22.7 μM, respectively).