• Particle and aerosol research
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• v.19 no.3
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• pp.77-87
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• 2023

We present the development of a wetcyclone sampler designed for the sampling of airborne bacteria. The wetcyclone design involves a combination of two traditional cyclone shapes and computational fluid dynamics (CFD) analysis to validate its effectiveness in terms of pressure drop and collection efficiency. The wetcyclone exhibits a collection efficiency of over 90% for bacteria, specifically targeting Staphylococcus aureus. Additionally, the wetcyclone enables continuous bioaerosol sampling using a liquid medium (deionized water), demonstrating a concentration ratio exceeding >105 and a stable microbial recovery rate of 81.9%. The application of real-time quantitative polymerase chain reaction (qPCR) and the colony counting method ensures precise measurement of the concentration ratio and microbial recovery rate.

• Journal of Microbiology and Biotechnology
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• v.33 no.10
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• pp.1292-1298
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• 2023

PAMB 00755^T, a bacterial strain, was isolated from Korean fir leaves. The strain exhibits yellow colonies and consists of Gram-negative, non-motile, short rods or ovoid-shaped cells. It displays optimal growth conditions at 20℃, 0% NaCl, and pH 6.0. Results of 16S rRNA gene-based phylogenetic analyses showed that strain PAMB 00755^T was most closely related to Sphingomonas chungangi MAH-6^T (97.7%) and Sphingomonas polyaromaticivorans B2-7^T (97.4%), and ≤96.5% sequence similarity to other members of the genus Sphingomonas. The values of average nucleotide identity (79.9-81.3%), average amino acid identity (73.3-75.9%), and digital DNA-DNA hybridization (73.3-75.9%) were significantly lower than the threshold values for species boundaries; these overall genome-related indexes (OGRI) analyses indicated that the strain represents a novel species. Genomic analysis revealed that the strain has a 4.4-Mbp genome encoding 4,083 functional genes, while the DNA G+C content of the whole genome is 66.1%. The genome of strain PAMB 00755^T showed a putative carotenoid biosynthetic cluster responsible for its antioxidant activity. The respiratory quinone was identified as ubiquinone 10 (Q-10), while the major fatty acids in the profile were identified as C_18:1ω7c and/or C_18:1ω6c (summed feature 8). The major polar lipids of strain PAMB 00755^T were diphosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid, and phosphatidylcholine. Based on a comprehensive analysis of genomic, phenotypic, and chemotaxonomic characteristics, we proposed the name Sphingomonas abietis sp. nov. for this novel species, with PAMB 00755^T as the type strain (= KCTC 92781^T = GDMCC 1.3779^T).

• The Transactions of the Korea Information Processing Society
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• v.6 no.3
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• pp.710-721
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• 1999

In this paper, we have designed and implemented a parameter extraction system for analyzing Internet using SNMP. The extraction system has two modules; one is collection request module, and the other is analysis request module. The collection request module generates a polling script, which is used to collect management information from the managed system periodically. With this collected data, analysis request module extracts analysis parameters. These parameters are traffic flow analysis, interface traffic analysis, packet traffic analysis, and management traffic analysis parameter. For management activity, we have introduced two-step-analysis-view. One is Summary-View, which is used find out malfunction of a system among the entire managed systems. The Other is Specific-View. With this view we can analyze the specific system with all our analysis parameters. To show available data as indicators for line capacity planning, network redesigning decision making of performance upgrade for a network device and things like that.

• Korean Journal of Microbiology
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• v.54 no.4
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• pp.456-459
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• 2018

The genus of Olsenella has been isolated from vertebrate animal mouth, rumen, and feces. Olsenella sp. KGMB 04489 was isolated from fecal samples obtained from a healthy Korean. The whole-genome sequence of Olsenella sp. KGMB 04489 was analyzed using the PacBio Sequel platform. The genome comprises a 2,108,034 bp chromosome with a G + C content of 65.50%, 1,838 total genes, 13 rRNA genes, and 52 tRNA genes. Also, we found that strain KGMB 04489 had some genes for hydrolysis enzymes, and antibiotic biosynthesis and resistance in its genome based on the result of genome analysis.

• Journal of the Korean Society for information Management
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• v.34 no.1
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• pp.73-110
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• 2017

This study explores the effectiveness and applications of event collection programs, which are integrated programs with event experience and collection experience as a cooperation model that combines school education and library resources as course-related materials. For the purpose, a triangulated analysis with both quantitative and qualitative data was conducted. As a result, This study examined an event collection program's effectiveness which were positive effects on the participated students' reading and perceptions on the roles of their school libraries. The event collection supported the students to self-construct their knowledge by exploring information with the provision of a collection with various materials on a topic, which would be hardly provided within the traditional library subject classification, and that the event collection program could be a useful tool for self-directed reading due to its promotion of interaction, sharing, and participation among materials and the participated students by functional factors of experiential marketing.

• Journal of Korean Library and Information Science Society
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• v.48 no.1
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• pp.21-42
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• 2017

This study reports the result of the survey on collection management from 154 school libraries in Korea. The questionnaire is consisted of three section: state of collection, state of book use, and collection management system. Results reveal that (1) elementary schools and middle schools have many books for each student compared to the KLA's School Library Standards; (2) the component ratio of literature books is the biggest in school library collections; (3) the ratio of library book use is overwhelmingly higher in elementary schools than middle schools and high schools; (4) 64.6 percent of library books in the sample are never used during 2013-2015; (5) 74 percent of libraries have its own collection management document; (6) Almost all schools compose its own committee for library management, the committee plays an important roles in the communication among school members, collection development and promotion.

• Journal of the Korean Society for Library and Information Science
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• v.53 no.4
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• pp.189-211
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• 2019

The purpose of this study was to establish the concept of theme collection in the library, to clarify the reality of the theme collection operation. This study was to set up methodology for constructing and operating the theme collection in the future and to provide implications for future development. For this purpose, this study conducted in-depth interviews with a total of 12 directors and librarians from four public libraries. The results of the analysis revealed that the theme collection can be built and function properly with a solid understanding of its collection, users and community, and sharing its services goals and orientations. In addition, it was found that the task of constructing a theme collection contributes to the establishment of the identity of the librarian, and it is interrelated with the various library works. Finally, a comprehensive model of current theme collections was discussed.

• Journal of the Korean Society for Library and Information Science
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• v.52 no.4
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• pp.51-69
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• 2018

The purpose of this study is to analyze the potential use of the theme collection as a new classification method that reflects the interest of users in terms of classification and categorization. For this purpose, the background of the theme collection was identified based on the discussion of the library resource organization and the introduction of the curation service of bookstore. In addition, based on case analysis, which is building the theme collection, concrete concepts and characteristics of theme collection are derived. Based on the above discussion, the classification and categorization characteristics of public library themes collections were analyzed, and the characteristics and functions as a classification were compared with other categories relatively. Finally, the utility and applicability of the theme collection is presented and it is based on the discussions about the user-centered classification system design of the library in the future.

• The Plant Pathology Journal
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• v.40 no.1
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• pp.16-29
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• 2024

The Colletotrichum gloeosporioides species complex includes many phytopathogenic species, causing anthracnose disease on a wide range of host plants and appearing to be globally distributed. Seventy-one Colletotrichum isolates in the complex from different plants and geographic regions in Korea were preserved in the Korean Agricultural Culture Collection (KACC). Most of them had been identified based on hosts and morphological features, this could lead to inaccurate species names. Therefore, the KACC isolates were re-identified using DNA sequence analyses of six loci, comprising internal transcribed spacer, gapdh, chs-1, his3, act, and tub2 in this study. Based on the combined phylogenetic analysis, KACC strains were assigned to 12 known species and three new species candidates. The detected species are C. siamense (n = 20), C. fructicola (n = 19), C. gloeosporioides (n = 9), C. aenigma (n = 5), C. camelliae (n = 3), C. temperatum (n = 3), C. musae (n = 2), C. theobromicola (n = 2), C. viniferum (n = 2), C. alatae (n = 1), C. jiangxiense (n = 1), and C. yulongense (n = 1). Of these, C. jiangxiense, C. temperatum, C. theobromicola and C. yulongense are unrecorded species in Korea. Host plant comparisons showed that 27 fungus-host associations are newly reported in the country. However, plant-fungus interactions need to be investigated by pathogenicity tests.

• Journal of Microbiology and Biotechnology
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• v.15 no.3
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• pp.672-677
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• 2005

The standard reference collection of strains for E. coli, the ECOR collection, was analyzed by a genome-based typing method. Seventy-one ECOR strains were subjected to repetitive extragenic palindromic PCR genome fingerprinting with BOX primers (BOX-PCR). Using a similarity value of 0.8 or more after cluster analysis of BOX-PCR fingerprinting patterns to define the same genotypes, we identified 28 genotypes in the ECOR collection. Shannon's entropy-based diversity index was 3.07, and the incident-based coverage estimator indicated potentially 420 genotypes among E. coli populations. Chi-square test of goodness-of-fit showed statistically significant association between the genotypes defined by BOX-PCR fingerprinting and the groups previously defined by multi-locus enzyme electrophoresis. This study suggests that the diversification of E. coli strains in natural populations is actively ongoing, and rep-PCR fingerprinting is a convenient and reliable method to type E. coli strains for the purposes ranging from ecology to quarantine.ine.