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Fine mapping of rice bacterial leaf blight resistance loci to major Korean races of Xoo (Xanthomonas oryzae)

  • Lee, Myung-Chul;Choi, Yu-Mi;Lee, Sukyeung;Yoon, Hyemyeong;Oh, Sejong
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.10a
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    • pp.73-73
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    • 2018
  • Bacterial leaf blight(BLB), caused by X. oryzae pv. oryzae(Xoo), is one of the most destructive diseases of rice due to its high epidemic potential. Understanding BLB resistance at a genetic level is important to further improve the rice breeding that provides one of the best approaches to control BLB disease. In the present investigation, a collection of 192 accessions was used in the genome-wide association study (GWAS) for BLB resistance loci against four Korean races of Xoo that were represented by the prevailing BLB isolates under Xoo differential system. A total of 192 accessions of rice germplasm were selected on the basis of the bioassay using four isolated races of Xoo such as K1, K2, K3 and K3a. The selected accessions was used to prepare 384-plex genotyping by sequencing (GBS) libraries and Illumina HiSeq 2000 paired- end read was used for GBS sequencing. GWAS was conducted using T ASSEL 5.0. The T ASSEL program uses a mixed linear model (MLM). T he results of the bioassay using a selected set of 192 accessions showed that a large number of accessions (93.75%) were resistant to K1 race, while the least number of accessions (34.37%) resisted K3a race. For races K2 and K3, the resistant germplasm proportion remained between 66.67 to 70.83%. T he genotypic data produced SNP matrix for a total of 293,379 SNPs. After imputation the missing data was removed, which exhibited 34,724 SNPs for association analysis. GWAS results showed strong signals of association at a threshold of [-log10(P-value)] more than5 (K1 and K2) and more than4 (K3 and K3a) for nine of the 39 SNPs, which are plausible candidate loci of resistance genes. T hese SNP loci were positioned on rice chromosome 2, 9, and 11 for K1 and K2 races, whereas on chromosome 4, 6, 11, and 12 for K3 and K3a races. The significant loci detected have also been illustrated, NBS-LRR type disease resistance protein, SNARE domain containing protein, Histone deacetylase 19, NADP-dependent oxidoreductase, and other expressed and unknown proteins. Our results provide a better understanding of the distribution of genetic variation of BLB resistance to Korean pathogen races and breeding of resistant rice.

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A Study of the Nursing Needs of Hospitalized medical Patients by Means of Nursing History Form (간호력(Nursing History)을 통해 본 내과 입원환자의 간호요구)

  • 전경애
    • Journal of Korean Academy of Nursing
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    • v.7 no.2
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    • pp.1-10
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    • 1977
  • Personalized Patient centered nursing care is increasingly difficult to achieve despite the fact that it remains one of our consistent goals. So, we must find away to individualized nursing care. One means, to achieve this is by use of the nursing history form, which has been developed to help the nurse make maximum use of her limited time with the patient, by obtaining systematically the information needed to plan his nursing care. The nursing history form can be used to collect data about individual nursing needs but also it lends itself to the collection of epidemiological data relevant to the needs of patient population. So this study was undertaken in an attempt to describe the general characteristics of the population studied, to find out their perceptions and expectations related to their illness and hospitalization, to find out specific basic needs and to examine the relationship between the patients nursing needs and demographic characteristics through the responses to the nursing history questions. The study population defined and selected was all the patients (70) who were admitted to Yonsei University Hospital from October 1 - 15, 1975. The direct interview method was used and the data were categorized by the investigator, according to the nature of responses to each question and were subjected to the percentile and the chi- square tests. The findings can be summarized as follows 11. General characteristics of the study population ; The population was made up mainly of urban patients who were highly educated. The age was equally distributed. 2. Patients, perceptions and expectations related to illness and hospitalization ; 88.6% of the patients knew about the reason for hospitalization but 64. 5% could state symptoms only. 37.5% recognized the cause of illness. Approximately three fourth of the patients expressed on expectation for early recovery. 60.0% of the patient responses indicated, that they expected less than 10 days of hospitalization. Of the total responses regarding hospitalization, 45.7% were negative about the post -hospitalization expectation, 45.7% expected to return to work. As a result of these findings, we can see that there is a great educational need, a psychological need and environmental need for adaptation to the hospital and a socio- economic need for the post - hospitalization period. 3. Specific basic needs : The physical needs include the problem of getting sufficient sleep (50.0%), difficulty in food intake(47.1%), problems with hospital diet (47.0%), abnormal condition of the skin(44.3%), problems with bowel elimination(35.7%), assistance with bathing(35.7%), pain(30.0%), difficulty in walking(30.0%) , difficulty in seeing(30.0%) problems in urinary elimination(20.0%) , and difficulty in hearing(10.0%), 4. Nursing needs and epidemiological characteristics ; Age distribution was related to the rendition of the mouth but no significant differences were observed statistically with the patients responses to the other nursing history questions according to the epidemiological variables of age, sex, education and residence.

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A Study on the Curriculum Development for the Professional Hospice Nurse in Korea (한국 호스피스 전문간호사 교육과정 개발을 위한 기초조사)

  • 최의순;노유자;한성숙;김남초;김희승;박호란;안성희
    • Journal of Korean Academy of Nursing
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    • v.28 no.4
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    • pp.1027-1035
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    • 1998
  • The purpose of this study is to develop the first domestic professional hospice educational program. We investigated the present condition of Korean hospice education and analyzed the prerequisite need for a dedicated hospice course in the professional education process. Research was conducted between June and November 1996 for nursing professors teaching at each nursing education institute to find out how much hospice is being discussed and by whom, in which course it is being discussed, and also to find out the contents that needed to be included in the professional education process. From a total of 49 colleges(29 three year colleges, 20 four year colleges) out of 99, 162 nursing professors replied, the collection rate was 49.5%. The conclusions are as follows ; 1. The present condition of the hospice nursing education. 1) Whether hospice is included in the education program. \circled1 89.65% of 3 year colleges and 90% of 4 year colleges included hospice education in their education program. \circled2 In graduate studies three schools included hospice in their program and three schools expressed their plans to include hospice education 2) Hospice related education were commonly discussed in adult nursing(26.3%), fundamental nursing(22.8%), and psychiatric nursing(20.2%). In 3 year colleges its commonly discussed in the first and second year and in 4 year colleges it is taught in the second and third year. 3) Hospice related theory/practical education hours were averages of 6.5/7.0 hrs in 3 year colleges and 14.2/11.3 hrs in 4 year colleges. 4) The majority of professors in charge of hospice education were in the following order adult nursing, psychiatric nursing, and fundamental nursing. 5) The courses that are thought to be adequate to manage hospice related education were adult nursing(29.3%), community health nursing(21.7%) and the desired method of education was the method currently being used (36.5%). 2. The demand for hospice nursing education. 1) Over 70% demanded professional hospice education program, the highest demand was for the value and meaning of life followed by the role and qualification of the hospice team and the mental maintenance of a dying patient. 25 categories showed over 90% demand. 2) The highest demand was for the value and meaning of life (98.2%) and the lowest demand were for danjeon breathing(71.0%)and acupuncture(71.0%). 3) Other contents that need to be discussed in the professional hospice education program were hospice nursing, the attitude and reaction of death, bereavement care, and the prospects of hospice.

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The Effect of Mother-fetus Interaction Promotion Program of Talking and Tactual Stimulation on Mother-fetus Interaction and Mother-infant play Interaction (태담과 촉각 자극의 모-태아 상호작용 증진 프로그램이 모아 상호작용에 미치는 효과)

  • Kim, Jung-Soon
    • Korean Parent-Child Health Journal
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    • v.5 no.2
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    • pp.253-276
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    • 2002
  • Mother and infant relationship has a great influence on child's developments. Expecially, mother and infant relationship is affected by mother and infant interactions during one year after birth and an omen of mother-infant interaction after birth is mother-fetus interaction. This study was conducted to develop mother-fetus interaction promotion program of talking and tactual stimulation aimed at enhancing sensitivity of primiparas and to evaluate the effect of the nursing intervention program on mother-fetus interaction and mother-infant play interaction. Non-eguivalent control group posttest design was used, and Blumer's symbolic interaction theory and Barnard's mother-infant interaction model was used a conceptual framework of this study. Fifty primiparas and infant(26 mother-infant dyads for intervention group and 24 dyads for control group) were recruited from three general hospital and an OBGY clinic located in Kwang Nung city. Data was collected from January 30th to December 20th in 2001. For the intervention group, programed education which focused on mother-fetus interaction in the 1st trimester. Telephone counselling was provided with interval of two weeks. For two groups, home visiting for data collection of mother- fetus interaction was conducted at 36 to 38 weeks of gestational age. And mother-infant play interactions were assessed at 4 to 6 weeks after birth using videotapes. Play situations were videotaped and two trained observers analyzed the tapes. The data were analyzed using chi-square test and Fisher's Exact Test to test the equivalence of two groups, and the effect of intervention progrom was determined with t-test and Wilcoxon Rank Sum test. The results were as follows: 1. The significant difference was found in mother-fetus interaction between two groups(t=2.085, P=0.0425). It indicated that intervention progrom was effective in improving mother-fetus interaction. 2. The significant difference was found in mother-infant play interaction between two groups(W=347.5, P=0.0001). In subscale analysis, three subscales showed significant differences between the groups: interactional behavior of mother(t=5.921, P=0.0001), interactional behavior of infant(t=5.736, P= 0.0000), and synchronic interactional behavior of mother and infant(t=7.940, P= 0.0000). In conclusion, this study has shown that the applied nursing intervention aimed at enhancing sensitivity of primiparas promoted mother-fetus interaction and mother-infant play interaction. Therefore, this study suggests that this nursing interventions to increase maternal sensitivity to the fetus's movement should be broadly applied to primiparas, which can be beneficial for formation of mother - infant relationship, and for promotion the social, affective, and cognitive developments of their children.

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Pattern on Enterobius vermicularis in Korea (한국(韓國)에 있어서의 요충감염상(蟯虫感染相))

  • Lee, Joon-Sang;Kim, Kyu-Ho;Ryu, Jong-Cheol;Hong, Kyung-Sook;Lee, Hae-Jung;Rim, Han-Jong
    • Journal of agricultural medicine and community health
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    • v.3 no.1
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    • pp.18-26
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    • 1978
  • Enterobius vermicularis (pinworm) infestation in present day Korea has become one of nationwide problems. But, the incidence of pinworm infestation having been reported by many workers was almostly the data for specific groups. The incidence of pinworm infestation for 208 families of 760(male; 30, female;420) middle class inhabitant of Seoul, 238 subrural inhabitants in Gyeonggi Province and 150(male;77, female;73) rural primary students in three different districts were examined by modified Gralam's scotch-tape swab method. The results are summarized as follows; 1. The incidence of nationwide infestation a. The nationwide incidence of pinworm infestation of the 1,148 specimens examined was 28.7% (male; 27.5%, female; 29.6%). b. The highest incidence was found among 5-14 years of age. c. In comparison of the incidence by the area, the highest incidence was found among subrural area, next among rural area and the lowest among urban area. 2, Urban area a. The incidence of the 760 inhabitants examined was 23.2% (male; 24.1%, female; 22. 3%). b. The incidence of the parents group was 12.2% and children group was 30. 30.3%. c. In the parents who hold one or more pinworm poitive cases among children, the incidence of father group was 13.2% and mother group was 29.7%.3. Subrural area a. The incidence of the 238 inhabitants examined was 42.9% (male, 40.8%, female; 44.4%). b. The cumulative positive rate of anal swabs showed increasing tendency. c. Worm collection rates after chemotherapy of 98 inhabitants were 54, 0%. d. In scotch tape anal swab only one times, diagnostic value was 74.0%. e. Mother group infested with pinworm seemed to play an important role for the high incidence in individual family. f. In comparison of the incidence by the examination method, the highest incidence was found among scotch tape method. 4. Rural area The incidence of the 150 primary students examined was 34. 5% (male ; 24. 7%, female; 43.8%).

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A Study on the Introduction of Electronic Commerce for Purchasing Section in Hospitals - Focused on the Understanding of Purchasing Managers - (병원구매업무에 있어서의 전자상거래 도입에 관한 연구 - 구매부서 관리자의 인식도를 중심으로 -)

  • Hwang, Eun-Bum;Nam, Sang-Yo;Ha, Ho-Uk;Lee, Chang-Eun
    • Korea Journal of Hospital Management
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    • v.6 no.3
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    • pp.69-89
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    • 2001
  • This study is focused that the electronic commerce(EC) on the purchasing section may improve the efficiency and transparency of the hospitals management. After reviewing the purchasing activity of hospitals, I study the introduction, expected effects, and problems of EC. So, I am going to provide basic information for activating EC. The samples are managers of 170 hospitals, which are located on Seoul. As a result of collection this survey, I analyze 79 hospitals. For data analysis, I use $X^2$-test and ANOVA for purchasing management and the relevance of EC according to the level of care. The results of this study are 1. The problems on the management of purchasing section are: firstly, they don't have sufficient time to study market. Secondly, it is difficult to find competitive suppliers. And, lastly, they cannot gather a lot of information about the price of products. 2. There are many answers of the needs on the introduction of B2B. However, some hospitals think they don't need it. But, the most answers are that the EC will be settled within 4 years. So, we can realize that these hospitals are getting interested on the EC. On the other hand, I find that they prefer outside EC companies for the introduction of EC. 3. On the expected effects on EC, first is the effectiveness of the market survey. The next is to collect information of adequate price of products owing to clear transaction, find easier new suppliers and gather useful data. 4. On the external problems of the introduction of EC, there is low credibility related to the security and the weakness of suppliers' information system. Especially, on the Real Transaction Price Payment system, the bigger bed size, the higher understanding on these problems. On the internal problems of the introduction of EC, first is the burden of the introduction of EC and operating cost. Especially, on the burden of the disclosure of revenue source, the smaller bed size, the higher understanding on this problem So, this is a point which deserves my attention statistically. However, this shows relatively little understanding about incomplete the standard of product category and the weak information system of hospital. Through this study, I am going to suggest 3 points for the activation of the introduction of EC on hospitals. 1. The reform of the Real Transaction Price Payment System on medical supplies and materials for medical treatment 2. The establishment of the standard of product category 3. The promotion of information system based on network.

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Nursing Faculty Competence and Nursing Faculty's Attitude for Lecture Evaluation (간호학교수의 교수능력과 강의평가에 대한 태도조사)

  • Suh, Moon-Ja;Do, Bok-Neum;Park, Chun-Ja;Kim, Kem-Soon;Im, Nan-Young;Choi, Kyung-Sook;Lee, Sun-Ok;Kim, Soon-Re;Choi, S-Mi;Park, Young-Im;Choi, Sang-Soon;Jung, Eun-Soon;Cho, Nam-Ok
    • The Journal of Korean Academic Society of Nursing Education
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    • v.9 no.2
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    • pp.320-328
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    • 2003
  • Nurse educators' competence is considered to be important in relation to the quality of education for nursing students. The purpose of this study was to identify the nursing faculty competence and attitude for lecture evalutaion, evlauated by nursing faculty. This article presented results focusing on South Korean nursing faculty's attutude for lecture evalutaion done by students and their opinion of the importance of nurse educator competence domains. The data collection was done from October, 2002 to August, 2003 and 151 subjects were used for the analysis. The Ideal Nursing Teacher Questionnaire was used to assess nurse faculty competence. The results showed that all of the lecture were evaluated and 59.6% of clinical practice was evaluated by nursing studuents. Nursing faculty thought that lecture evaluation by students was resonable, even though it was stressful. The most important among 5 domains of nurse educators' competence assessed by nursing faculty was nursing competence. Moreover nursing faculty responed that teaching skills domain and evaluation skills domain were more important than relationship with students domain and personality factors domain. Compared to young nursing faculty, nursing faculty who was old aged reported that nurse educators' competences in 4 domains of nursing competence, teaching skills, evaluation skills and relationship with students were more important.

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A Study on Training Plan of Policy Information Librarian (정책정보전문사서 양성방안에 관한 연구)

  • Noh, Younghee
    • Journal of the Korean BIBLIA Society for library and Information Science
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    • v.27 no.1
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    • pp.27-57
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    • 2016
  • This study was designed to derive and propose the qualities, qualifications, duties, etc. needed for policy information librarians who work in the policy-related special libraries. To do this, literature related to professional librarians was analyzed comprehensively with respect to qualifications, qualities, duties presented and needed in domestic and international jobs sites. The information derived through this process was developed into a questionnaire and a survey conducted targeting as subjects specialized librarians in related fields. The results indicated that the highest required LIS degree for both policy information librarian and for policy related fields is a Master's Degree. Additionally in investigating the importance of related career experience required for a policy information librarian, work related to policies reference services appeared to be the most important. On the issue of important qualities required of a policy information librarian, 'outstanding policies related data navigation capabilities' was the highest, followed by 'knowledge of information policy and knowledge of policy related resources', 'knowledge of the current trends of policies related fields', and 'smooth interpersonal skills.' The survey also indicated that with respect to the duties of the information librarian planning of policy information services had the highest score, followed by the providing of policies information services, and then collection management.

A Study on the Lived Experiences of Homecare Nurses (가정간호사의 실무체험 연구)

  • 서문자;김소선;신경림;강현숙;김금순;박호란;김혜숙
    • Journal of Korean Academy of Nursing
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    • v.30 no.1
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    • pp.84-97
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    • 2000
  • The Necessity and Purpose of the Study Recently the number of patients with chronic diseases and the aged patients is increasing steadily. Furthermore, due to the expansion of health insurance system, the number of patients hospitalized in the general hospital is increasing at a surprising speed. However, hospitals urge the early discharge of the patients for the efficiencies of hospital administration, and therefore, the number of patients who must be taken care of in their home is also increasing. Homecare nursing is one of the health care service for the patients at home who require continual attention and care, and now increasing attentions are given to it as one of the professional nursing fields. However, it was almost impossible to find a study on the actual experiences of the homecare nurses written by their own language in Korea, that it also posed a great difficulty in understanding their diverse experience. Considering these situation, this study will help understanding of them, and provide the fundamental data on their experiences for making policies to develop homecare nursing. Methods of Research Phenomenological research method was employed to analyze the lived experiences of homecare nurses fundamentally. Data collection Data were collected from August 1998 to December 1998 from ten homecare nurses who worked for patients under the homecare nursing setting as model cases designated by Seoul Nurses Association and who agreed to the purpose of this study after listening to and understanding the explanation completely. The in-depth interview was carried at the time which was convenient both for the researcher and participants for one or two hours, and recovered with the approval participants. The first interview covered diverse and broad areas like the situation of homecare nursing, and their feelings and thoughts over it, and in the second and third interviews, more specific questions are asked. Data Analysis For the phenomenological analysis, contents analysis was employed. The data collected from the participants were analyzed into the following procedures according to Van Manen 's phenomenological analysis. 1) Reserve the preconception of the researcher by restricting it inside parenthesis. 2) Make a thorough observation of the lived experiences by insight process. 3) Analyze the contents (Find out the repetitive factors) 4) Interpret the essence found. 5) State the meaning of the interpretation. Results and discussion 1. Fear and expectation for the first visit. (unfamiliarity, awkwardness, anxiety, shivering) 2. Mingle with the family (feeling friendly with the family, becoming like a family member) 3. Being proud of her own know-how (learning the know-how, organizing alternatives, building up confidence) 4. Pity for the poor. (criticizing the current government, feeling ashamed, feeling anger) 5. Difficulty of constructing cooperative system with physicians (strenuousness, frustration) 6. Helplessness due to the lack of support system (difficulty to get supplies, annoyance, embarrassment by institutional restraints) 7. Anxiousness for heavy traffic and parking (annoyance, hastiness) 8. Ethical conflicts (pity for the patients and family, skepticism about lengthening life maintenance) 9. Burden for the possible accident (pressure, anxiety, conflict, physical exhaustion) 10. Establishment of identity as a professional (fulfillment, worth, joy) 11. Being distressed at other's ignorance

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The Roles of Critical Care Advanced Practice Nurse

  • Sung, Young-Hee;Yi, Young-Hee;Kwon, In-Gak;Cho, Yang-Ae
    • Journal of Korean Academy of Nursing
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    • v.36 no.8
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    • pp.1340-1351
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    • 2006
  • Purpose. To determine and compare the perception among nurses and doctors of the roles and tasks of critical care advanced practice nurses (APNs) in order to establish standardized and formally agreed role criteria for such critical care APNs. Method. This study measured and analyzed the necessity of each of the roles and tasks of critical care APNs, as perceived by nurses and doctors, through a survey of 121 participants: 71 nurses in 7 intensive care units (ICUs) at a general hospital in Seoul, and 50 doctors who used ICUs. Data collection utilized a questionnaire of 128 questions in the following fields: direct practice (79), leadership and change agent (17), consultation and collaboration (15), education and counseling (11), and research (6). Results. Both the nurses' and the doctors' groups confirmed the necessity of critical care APNs, with doctors who frequently used ICUs indicating a particularly strong need. As for the priority of each role of critical care APNs, the nurses considered direct practice to be the most critical, followed by education and counseling, research, consultation and collaboration, and leadership and change agent. The doctors also considered direct practice to be the most critical, followed by education and counseling, consultation and collaboration, research, and leadership and change agent. There was a statistically significant difference between how the two groups regarded all the roles, except for the consultation and collaboration roles. As for the necessity of each role of critical care APNs, the nurses considered research to be the most necessary, followed by education and counseling, consultation and collaboration, leadership and change agent, and direct practice. The doctors, on the other hand, considered education and counseling to be the most necessary, followed by research, consultation and collaboration, leadership and change agent, and direct practice. The responses of the two groups to all the roles, except for education and counseling roles, were significantly different. Conclusion. Nurses and doctors have different perceptions of the roles and tasks of critical care APNs. Thus, it is necessary for the combined nursing and medical fields to reach an official agreement on a set of criteria to standardize for the roles and tasks of critical care APNs.