• 대한화장품학회지
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• 제25권4호
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• pp.145-154
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• 1999

A novel retinol derivative, polyethoxylated retinamide(Medimin A) was synthesized, as an anti-aging agent. Collagen synthesis, skin permeation, stability, and toxicity of Medimin A were evaluated and compared with those of retinol and retinyl palmitate. In vitro collagen synthesis was evaluated by quantitative assay of $[^3H]-proline$ incorporation into collagenase sensitive protein in fibroblast cultures. For in vitro skin permeation experiments, Franz diffusion cells(effective diffusion area: 1,766 $cm^2$) and the excised skin of female hairless mouse aged 8 weeks were used, The stabilities of retinoids were evaluated at two different temperature($25^{\circ}C\;and\;40^{\circ}C$) and under UV in solubilized state and in O/W emulsion. To estimate the safety, acute oral toxicity, acute dermal toxicity, primary skin irritation, acute eye irritation and human patch test were performed. The effect of Medimin A on collagen synthesis was similar to that of retinol. The skin permeability of Medimin A was higher than those of retinol and retinyl palmitate. The Medimin A was more stable than retinol and retinyl palmitate. Medimin A was nontoxic in various toxicological tests. These results suggest that Medimin A would be a good anti-aging agent for enhancing bioavailability and stability.

• 한국융합학회논문지
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• 제12권1호
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• pp.251-257
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• 2021

본 연구에서는 천연재료인 편백나무 수피에서 추출한 천연추출물이 자외선에 의한 피부 노화를 보호할 수 있는 소재로 조사하였는데, 피부의 천연보습인자를 구성하는 필라그린(filaggrin)의 합성과 진피의 보습에 중요한 역할을 하는 섬유단백질인 Pro-collagen의 합성과 콜라겐(collagen)을 분해하는 효소의 저해효과 및 탄력섬유인 엘라스틴(elastin)의 분해효소 저해 실험을 진행하였다. 결과적으로 편백에탄올추출물은 collagenase와 elastase를 농도의존적으로 저해하였으며, 자외선에 손상을 받은 각질형성세포에 대한 filaggrin의 합성과 MMP-1의 발현을 억제하였다. 따라서 이런한 결과는 에탄올추출물(COE)이 주름생성을 지연시키는 역할과 피부노화 융합 억제 하는 기능성 화장품 소재로서의 효과를 가질것으로 사료된다. 본 연구를 바탕으로 filaggrin의 합성이 항주름 효과인 MMP의 발현억제에 미치는 기전을 더 연구하고자 한다.

• 한국식품영양과학회지
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• 제35권1호
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• pp.42-47
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• 2006

본 연구에서는 비타민 C의 배양세포 증식을 촉진하는 기능을 세포 간 결합조직의 주성분인 콜라겐 생합성에 초점을 맞추어 3T6 세포주 및 흰쥐에서 분리한 초대 연골세포를 이용하여, 세포 및 세포 외액 중의 콜라겐 함량의 분석과 콜라겐 생합성에 미치는 비타민 C의 영향을 각 농도별로 분석하여 콜라겐 생합성에 필요한 비타민 C의 적합한 첨가 농도를 검토하였다. 비타민 C를 1.0 mM 농도가 되도록 첨가하여 3T6 섬유아세포 및 초대연골 세포를 배양하였을때 양 세포 모두 콜라겐 양은 비타민 C를 첨가한 세포가 높은 수치를 나타내어 비타민 C에 의한 콜라겐 합성의 촉진효과가 현저하였으며 일수의 증가에 따라 그 합성량도 증가하였다. 비타민 C 무첨가의 경우 세포층의 콜라겐 합성량을 3T6 섬유아세포와 연골세포를 비교하였을 때 3T6 섬유아세포의 경우 배양일수의 경과와 더불어 콜라겐 양이 증가하였으나 연골세포의 경우 1일째부터 21일째까지 콜라겐양은 거의 변화하지 않아, 본 실험에 사용한 연골세포는 초대 배양세포로서 세포외로부터 비타민 C의 공급이 되지 않을 경우콜라겐 합성은 일어나지 않는 것으로 사료된다. 따라서 배양세포의 콜라겐 합성에 미치는 비타민 C의 영향을 검토하기 위하여 초대연골세포를 이용하여 비타민 C의 농도를 변화시켜 가면서 콜라겐 합성량을 측정하였다. 5 mM, 7 mM, 10 mM의 고농도의 비타민 C를 첨가한 경우 저 농도의 비타민 C 첨가 경우보다 plate 내의 총 콜라겐 함량은 적었다. 그러나 DNA 양에 대한콜라겐 함량을 비교하였을 때 5mM 이상의 비타민 C 첨가에서는 콜라겐 합성 증가량이 조금 낮은 경향을 보였으나, $0.1\~2mM$을 첨가하였을 때의 콜라겐 양과 최종적으로는 거의 비슷한 수치를 나타내었다. 0.5 mM 이상의 비타민 C 첨가는 콜라겐 합성을 저해한다는 기존의 보고와는 달리 본 연구에서는 세포독성을 억제할 목적으로 배지 중에 catalase 첨가하였으며, 그 결과 $0.1\~10mM$의 비타민 C 농도범위에서는 콜라겐 합성량에 차이가 크게 없는 것으로 나타나, $0.1\~10mM$의 비타민 C 농도로는 catalase를 첨가한 배지를 사용할 경우 세포내의 콜라겐 양에 대한 비타민 C 첨가농도별 차이는 크게 없는 것으로 추측된다.

• Asian-Australasian Journal of Animal Sciences
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• 제6권4호
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• pp.483-489
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• 1993

Bovine fibroblasts were cultured in Dulbecco's Modified Eagle's Medium and then treated with control, insulin (I, $1{\mu}g/ml$), cholera toxin (CT, 0.1-100 ng/ml) or CT (0.1-100 ng/ml) + I ($1{\mu}g/ml$). Cholera toxin, an activator of adenylate cyclase, significantly decreased insulin induced DNA synthesis (p<0.05). The modulation of DNA synthesis apparently involves events occurring in early stage of cell growth, at least between the first 4 and 8 hour of CT treatment. Insulin induced collagen as well as noncollagen synthesis in cell layer, however, these syntheses were reduced by addition of cholera toxin (p<0.05) but were not completely reduced. It is not clear whether the reduction of insulin-induced cell layer collagen or noncollagen proteins by CT is involved in the inhibitory effect on insulin-induced DNA synthesis. However, we could rule out the hypothesis that insulin-induced DNA synthesis is reduced by CT-induced cellular differentiation.

• 대한예방한의학회지
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• 제10권2호
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• pp.19-30
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• 2006

It is well known that glucocorticoid may induce osteoporosis as its side effect in long-term therapy. The inhibition of osteoblast by glucocorticoid is also recognized as its action mechanism of decreased bone formation. In this study, the effect of DSG, Danchisoyosangamibang, on the differentiation and function of osteoblastic cells was investigated. The osteoblastic cells were isolated from rat calvariae using collagenase treatment. The cell counting, enzyme activity assay, MTT assay, collagen content assay were done to determine the cell proliferation, intracellular alkaline phosphatase (ALP) activity, bone martrix production, and cell apoptosis. DSG enhanced the cell proliferation after the culture for 10 days. ALP activity and total protein synthesis, and intracelluar collagen synthesis were increased time dependently when the cells were treated with DSG in the presence of dexamethasone. And, DSG restored calvarial cell function decreased by dexamethasone.

• 대한물리치료과학회지
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• 제8권1호
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• pp.727-734
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• 2001

Contracture is defined as the lack of full passive range of motion resulting from pint, muscle or soft tissue limitationprolonged Pint immobilization will result in stress and stretch deprivation and gradual development of contracture. the tissue changes caused by immobilization may be categorized as cellular modeling, ground substance and collagen response, and tissue response. contracture can be divided into three categories according to the anatomical location of pathological changes :arthrogenic, myogenic, soft tissue contractures Therapeutic approach of contracture is thermal or cold agents application, stretch or restoration of length, traction, manipulation, mobilization positioning and restoration of function. The purpose of this article is to review current concepts of mechanical properties and synthesis of collagen tissue and the underlying pathomechanics as it relates to evaluation and treatment of contracture.

• 대한예방한의학회지
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• 제12권2호
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• pp.197-206
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• 2008

The inhibition of osteoblast by glucocorticoid is recognized as its action mechanism of decreased bone formation. In this study, the effect of JY, Jahyulyangkeuntang, on the differentiation and mineralization of osteoblastic cells was investigated in the presence of dexamethasone. The cell counting, enzyme activity assay, MTT assay, collagen content assay were done to determine the cell proliferation, alkaline phosphatase(ALP) activity, bone martrix production, and cell apoptosis. JY enhanced the cell proliferation after the culture for 10 days. ALP activity and total protein synthesis, and intracellular collagen synthesis were increased when the cells were treated with JY. And JY restored calvarial cell function decreased by dexamethasone.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.238.1-238.1
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• 2003

Hepatic stellate cells (HSC) and the derived myofibroblasts are known to play a central role in liver fibrogenesis. Rhus verniciflua Strokes (RVS) has traditionally been used in Korea herbal medicine for a stomachic tonic. In this study, we observed the effect of RVS acetone extract (Ra) and its five major components on the proliferation, the collagen synthesis, and hepatic fibrosis related proteins mRNA levels in HSC-T6 cells which is a fully activated rat hepatic stellate cell line. Ra inhibited the proliferation and decreased the content of collagen in the HSC-T6 cells. (omitted)

• 한국융합학회논문지
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• 제11권5호
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• pp.61-72
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• 2020

본 연구에서는 어류 유래 콜라겐 가수분해물로부터 한외여과법(MWCO; 1 kDa)과 역상액체크로마토그래피를 이용하여 항노화 활성 기능성 펩타이드를 분리, 정제하고자 하였다. 펩타이드의 분리, 정제에 따른 항노화 활성 변화는 Hs68 세포를 사용하여 procollagen 합성능과 MMP-1 저해능을 측정하여 확인하였다. 콜라겐 가수분해물과 비교하여 최종 분리, 정제된 기능성 펩타이드의 procollagen 합성과 MMP-1 저해는 각각 46%와 77% 향상되었다. 또한 기능성 펩타이드의 구조는 Gly-Arg-Arg-Gly-Asn-Lys (GRRGNK; 콜라겐의 기본 구조인 Gly-X-Y sequence와 유사)의 서열을 보였고, 분자량은 686.175 Da으로 분석되었다. 따라서 본 연구에서는 어류 콜라겐 가수분해물로부터 분리한 펩타이드가 식품, 화장품, 의약품 등의 피부노화 지연 효과를 보이는 기능성 원료로 다양하게 사용할 수 있는 가능성을 확인하였다.

• Archives of Plastic Surgery
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• 제35권4호
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• pp.355-359
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• 2008

Purpose: Oncostatin M(OSM) has been known as a role in fibrosis and anti-inflammatory effects of various organs and tissues. Although there have been a number of studies which are focused on the roles and mechanisms of OSM, there are few reports on its effects in chronic wound healing. The purpose of this study is to evaluate the effects of OSM in wound healing activities of dermal fibroblasts of chronic wound in vitro. In particular, this study is focused on cell proliferation and synthesis of collagen and glycosaminoglycan(GAG), which are the major components of the extracellular matrices, of diabetic fibroblasts. Methods: Fibroblasts were isolated from excess skin that was obtained from diabetic foot ulcer patients who underwent debridement. The isolated fibroblasts were cultivated in presence of OSM(100 ng/mL). Cell proliferation, collagen synthesis and GAG levels were compared. Results: All the components tested in this study increased in OSM treatment group. In particular, collagen and GAG synthesis demonstrated statistically significant increases(p<0.05 in the Mann-Whitney U-test). Conclusion: These results indicate that OSM increases wound healing activities of dermal fibroblasts of chronic wound in vitro.