• 한국농공학회지
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• 제45권7호
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• pp.94-106
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• 2003

A pilot study was performed to examine the feasibility of UV disinfection system and the reactivation of indicator microorganisms (TC, FC, E. coli) after UV irradiation for agricultural reuse of reclaimed water. Photoreactivation and dark repair enable UV-inactivated microorganisms to recover and may reduce the efficacy of UV inactivation, which might be drawbacks of the UV disinfection method. The effluent of biofilter for 16-unit apartment house was used as input to the UV disinfection system, and average SS and BOD concentration were 3.8 and 5.7 mg/L, respectively, and the mean level of total coliform was in the range of $1.0\times10^4$ MPN/100mL. UV disinfection was found to be effective and it reduced mean concentration of indicator microorganisms (total coliform, fecal coliform, and E. coli) to less than 100 MPN/100mL within 60s exposure using 17, 25, and 40W lamps. Two UV doses of 6 and 16 mW$\cdot$s/$\textrm{km}^2$ were applied and microorganisms reactivation was monitored under the dark, photoreactivating light, and solar irradiation. Microorganisms reactivation was observed in the UV dose of 6 mW$\cdot$s/$\textrm{km}^2$, and numbers increased up to 5% at the photoreactivating light and 1% at the dark. However, microorganisms were inactivated rather than reactivated at the solar radiation and numbers decreased to non-detectible level about below 2 MPN/100mL in 4 hours. In the case of 16 mW$\cdot$s/$\textrm{km}^2$, microorganism reactivation was not observed indicating that UV dose might affect the reactivation process such as photoreactivation and dark repair. Therefore, concerns associated with microorganism reactivation could be controlled by sufficient UV dose application. Agricultural reuse of reclaimed water might be even less concerned due to exposure to the solar irradiation that could further inactivate microorganisms. The pilot study result is encouraging, however, sanitary concern in water reuse is so critical that more comprehensive investigation is recommended.

• 환경영향평가
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• 제16권4호
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• pp.241-250
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• 2007

A study was carried out on the level of heavy metals, total coliform and benzo(a)pyrene that may be introduced through by-product fertilizers or air pollution to turfgrass in natural turfgrass playground in elementary schools around the western coast and eastern mountain areas in Jeollabuk-do, Korea. The level of heavy metals found in turfgrass was in the order of Cu > Zn > Pb > Cd. The majority of heavy metals was found in the roots. It is presumed that the absorption of some air pollutants and by-product fertilizers or chemical fertilizers. The level of benzo(a)pyrene showed an average of 0.05ng/g with a range between 0.02 to 0.08ng/g in the leaves. In the meantime, none was detected in the stems, while the average for roots was 0.02ng/g with a range between 0.01 and 0.03ng/g. The level detected is that found background level, however, benzo(a)pyrene can be introduced partly from the unstable combustion of fossil fuel or through vehicle emissions. Total coliform distributed in grass showed an average of 12MPN/100ml with a range between 2 and 36MPN/100ml. The density of total coliform displayed no regional predominance. However, in some urban areas, the density was slightly higher. The feces of pets or by-product fertilizers may have contributed to the input of pathogenic micro-organism. Yet the level was found to be negligible.

• 한국식품위생안전성학회지
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• 제14권4호
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• pp.433-435
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• 1999

부산지역 4곳의 지하수와 시판생수 그리고 정수기를 통한 정수를 냉장과 실온에 보관하면서 일정기간별로 저장중에 따른 미생물의 변화를 연구한 결과 다음과 같이 나타났다. 지하수에서의 저장기간 동안 일반세균에 대한 변화는 최저 $10^2$~$10^3$CFU/m1 최고 $10^2$~$10^{5}$ CFU/m1의 범위로 나타났으며, 대장균에 대한 변화는 areaII를 제외한 지역의 시료에서 검출되었으며 7일차 경과 후 감소하는 경향을 보였다. 시판생수에서의 일반세균에 대한 변화는 최저10~$10^4$CFU/m1, 최고 $10^2$~$10^{5}$ CFU/m1의 범위로 나타났다. 대장균에 대한 변화는 제품I과 제품III에서는 검출되지 않았고, 제품II에서는 일반세균과 대장균이 냉장, 실온 모두에서 상당히 많은 수의 대장균이 검출되었으며, 7일차 이후에는 검출되지 않았다. 정수에서의 일반세균에 대한 변화는 냉장과 실온 각각 $10^3$~$10^4$CFU/m1, $10^2$~$10^4$CFU/m1의 범위로 나타났으며, 대장균은 전체보관기간동안 불검출로 나타났다.

• Fisheries and Aquatic Sciences
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• 제11권1호
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• pp.7-14
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• 2008

The sanitary quality of a shellfish-growing area in Kamak Bay, Korea, was assessed through a bacteriological examination of seawater and oysters from January 2004 to December 2006. From seawater samples collected at 28 stations, the range of geometric mean (GM) and the estimated 90th percentile for most-probable-number (MPN) values of fecal coliforms were <1.8-2.4 MPN/100 mL and 1.8-6.0 MPN/100 mL, respectively. The observed fecal coliform GM and the estimated 90th percentile did not exceed the fecal coliform water quality standards of 14 MPN/100 mL and 43 MPN/100 mL. Therefore, the bacteriological quality of seawater at this shellfish-growing area met the Korean Shellfish Sanitation Program (KSSP) criteria for a growing area used for export. The range of the fecal coliform GM and the estimated 90th percentile MPN values of oyster samples were 19.2-160 MPN/100 g and 20.2-166.9 MPN/100 g, respectively, and the range of the viable cell count was 30-1900 CFU/g. Thus, the fecal coliform value for the oysters and the viable cell count were less than the current shellfish quality standards of 230 MPN/100 g and 50,000 CFU/g, respectively. The bacteriological quality of the oysters complied with the criteria for domestic use and export of shellfish.

• 한국환경보건학회지
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• 제27권4호
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• pp.107-114
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• 2001

The number of covered carts selling snack foods along the streets are increasing in Korea and people enjoy eating foods at these carts. However there are only a few reports on the contamination or sanitary condition of the food carts. This study was performed to investigate the sanitary condition of food and water at the street food carts in the eight major areas of a city. Water and several kinds of food, kimbop(laver rollers) ddeokbokki(stir fried rice cake in hot pepper paste) oemuk(fish cakes) fish cake soup, and soy sauce were collected from four street carts from each of the 8 areas from June to August in 2001 The standard plate counts(SPCs) and coliform groups were examined according to the Food Code of Korea. The nufitness rates of SPCs of the samples were 0~15.6%: 15.6% in kimbop:6.3% in fish cake soup; and 3.1% in water samples SPCs were not detected in some samples. of ddeokbokki, oemuk and soy sauce. The unfitness rates of coliform groups were 0~62.5%; 46.9% in kimbop; 6.3% in ddeokbokki,;22.9% in oemuk 62.5% in fish cake soup; and 3.1% in soy sauce. Coliform groups were not detected in water sample The numbers and unfitness rates of SPCs and coliform groups showed increasing tendencies over time within a day. The higher the air temperature was the more increase of bacteriological growth was observed These results indicate that the level of bacteriological contamination of foods and water in the street carts should be monitored and strict inspection is necessary. There should be legal consequences for serving contaminated food to the public.

• 한국환경보건학회지
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• 제20권1호
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• pp.54-61
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• 1994

This study was carried out to investigate the degree of contamination in private-drinking water supply (PDWS) and simple-piped water supply (SPWS), and to compare of water quality between them for 3 years from 1990 to 1992. The results of the study were as follows: 1. Private-drinking water supply (PDWS) (1) Field-Site (ㄱ) The items which were very significantly over the criteria (p<0.01), were temp., pH, NO-N, standard plate count (S.P.C.) and Coliform. (ㄴ) No. of households which were over the criteria, were 11.2~51.7% in temp., pH, NH$_3$-N and NO$_2$-N, and 83.1~93.3% in NO-N, S.P.C. and Coliform. (2) Moutain-Site (ㄱ) The items which were over the criteria, were NO$_2$-N (p<0.05), S.P.C. (p<0.01) and Coliform (p<0.01). (ㄴ) No. of households which were over the criteria, were 33.6 ~ 75.6% in NO$_2$-N, S.P.C. and Coliform. (3) No. of households which were fit to the criteria of all items, were 9.0% in field-site and 14.1% in mountain-site. 2. Simple-piped water supply (SPWS) (1) The items which were very significantly over the critera (p<0.01), were standard plate count (S.P.C.) and Coliform. (2) The water qualities of adjacent and remote samples were better than those of source samples. (3) Residual chloride was not detected in any sample. In conclusion, rural community requires to be continually performed the policy of safety drinking water supply, and to be devised an epoch-making counterplan for the level-up of simple-piped water supply (SPWS), espically on the method of disinfection.

• 한국수산과학회지
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• 제45권5호
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• pp.460-464
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• 2012

From 1998 to 2008, the bacteriological quality of seawater in Kamak Bay, Korea, was monitored. The total and fecal coliforms in seawater were monitored as bacterial indicators, and the level of fecal coliform contamination was used to evaluate seawater quality. In the southern shellfish growing area of Kamak Bay, the observed fecal coliform geometric mean (GM) and estimated 90th percentile did not exceed the quality standards for fecal coliforms in seawater in Korea and th USA (GM<14 MPN/100 mL, 90th percentile<43 MPN/100 mL). The GM and estimated 90th percentile of the fecal coliform levels in the adjacent northern area of Kamak Bay were higher than in the southern area. Stations near wastewater discharge sites and urban areas in the north had the lowest quality and did not meet the bacteriological seawater quality criteria. The high fecal coliform levels in the Seonso coastal region near wastewater discharges and northern urban areas declined significantly following dredging and operation of a sewage treatment plant.

• Fisheries and Aquatic Sciences
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• 제22권11호
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• pp.26.1-26.7
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• 2019

Background: The monitoring of pathogens of fishery auction markets is important to obtain safe fishery products regarding hygiene and sanitation. In this study, aerobic, coliform, Escherichia coli, and Vibrio cholerae were monitored in the fishery products and environmental samples obtained from fishery auction markets. Methods: The fishery products (flounder, octopus, skate, rock cod, sea bass, snail, monkfish, flatfish, comb pen shell, corb shell, conger eel, hairtail, croaker, and pilchard) were placed in filter bags, and the environmental samples (samples from the water tanks at the fishery auction markets, seawater from the fishery distribution vehicles, ice from wooden or plastic boxes, and surface samples from wooden and plastic boxes used for fish storage) were collected. Aerobic bacteria, E. coli, and coliform in the samples were enumerated on aerobic count plates and E. coli/coliform count plates, respectively. For V. cholerae O1 and V. cholerae non-O1 quantification, most probable number (MPN)-PCR analysis was performed. Results: Aerobic and coliform bacteria were detected in most samples, but E. coli was not detected. Wooden boxes were contaminated with high levels of aerobic and coliform bacteria in all seasons (spring, summer, and fall). During fall, V. cholerae non-O1 were detected in snails, hairtails, croakers, flatfishes, pilchards, plastic boxes, and water samples. Conclusions: These results indicate an increased prevalence of V. cholerae contamination in fishery products in fall, including food contact samples, which can be vehicles for cross-contamination.

• 한국물환경학회지
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• 제28권6호
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• pp.890-895
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• 2012

Duration curves describe the percentage of time that a certain water quality (total/fecal coliform (=TC/FC)) or discharge is exceeded. The curves methodology are usually based on daily records and are useful in estimating how many days per year and event will be exceeded. The technique was further applied to estimated TC/FC loading to the Geumho River, using the daily mean flow rate and TC/FC concentration data during January, 2001 and December, 2011 for the Geumhogang6 (=Seongseo water level station) where an automated monitoring station is located in Gangchang-bridge. Low flow of the Seongseo (=11.1 cms) was equivalent to 75.3% on an exceedance probability scale. Load Duration curve for TC/FC loading at the Seongseo was constructed. Standard load duration curve was constructed with the water quality criteria for class III (TC/FC concentration = 5000/1000 CFU/ 100 mL). By plotting TC/FC observed load duration curve with standard load duration curve, it could be revealed that water quality do not meet the desired water quality for 68.8/11.2% on an exceedance probability scale. IF linear correlation between flow rate and coliform concentration is assumed, it can be interpreted that water quality exceed desired criteria when daily average flow rate is over 11.9/109.9 cms.

• 한국동물위생학회지
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• 제37권4호
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• pp.307-312
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• 2014

Fecal coliform bacteria are indicators of fecal contamination and of the potential presence of pathogens associated with animal excreta, wastewater, sludge. This study examined the distribution of fecal coliform in sand of the playground at educational facilities and parks in Seoul. Fecal coliform were detected from 61 (22.1%) places out of 276 playgrounds. Escherichia (E) coli (75.4%) was found most frequently, followed by Cronobacter (Cro) sakazakii (8.7%), Klebsiella (K) pneumoniae (7.2%) and Actinobacter baumannii (4.3%). We also tested the bacteria for their susceptibility to 16 antibiotics using disk diffusion method. All the isolates were highly susceptible to cefepime, gentamicin, tetracycline, ciprofloxacin, enrofloxacin, nalidixic acid and sulfamethoxazole/ trimethoprim, but resistant to apramycin and cephalothin. E. coli and Cro. sakazakii were highly resistant to apramycin (100% and 83.3%), followed by cephalothin (51.9% and 50%) and ampicillin (19.2% and 16.7%). K. pneumoniae was highly resistant to ampicillin and apramycin (100%, respectively) but the remaining antibiotics were susceptible.