• Title/Summary/Keyword: Coliform

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Changes in the Microbial Qualities and Sensory Characteristics of Boiled Quail Egg and Fish Paste in Soy Sauce as Prepared with the Cook-Chill System and Sous Vide Cook-Chill System (Cook-Chill System과 Sous vide Cook-Chill System으로 생산된 메추리알 어묵조림의 저장기간에 따른 미생물적 품질 및 관능특성의 변화(2))

  • Song, Sun-Mi;Kim, Heh-Young;Ko, Sung-Hee
    • Korean journal of food and cookery science
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    • v.23 no.2 s.98
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    • pp.261-269
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    • 2007
  • This study was performed to provide basic data for the sous vide cook-chill(SVCC) System by comparing and evaluating food quality, according to preparation method and days of storage, of foods that were prepared by SVCC to those prepared using the cook-chill(CC) System. Boiled quail eggs and fish paste in soy sauce were prepared using CC and SVCC and their quality was evaluated at the time of preparation and by days of storage. Viable cell counts were increased to 5.65(CC) and 3.40(SVCC) LogCFU/g by 15 days(p<.0001) and increased more over time in foods prepared with CC than with SVCC. For the CC method, Coliform counts increased to 4.58 LogCFU/g by 15 days(p<.0001). With SVCC, colirorms were not detected at 0 days, but counts reached 3.70 LogCFU/g by 15 days(p<.0001). After reheating, no coliforms were detected for CC or SVCC at 0 days, however, by 15 days, coliform counts reached 3.61 and 2.52 LogCFU/g(p<.0001) for CC and SVCC, respectively. Finally, the sensory scores of SVCC were higher than those of CC.

Study on the Systematic Technology of Promoting Purification for the Livestock Wastewater and Reuse

  • Okada, Yoshiichi;Shim, Jae-Do;Mitarai, Masahumi;Kojima, Takayuki;Gejima, Yoshinori
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 1996.06c
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    • pp.692-700
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    • 1996
  • The objective of this study is to develop a systematic purification plant using the metabolism of aerobic microorganisms. This system is subsequently aerated and continuously removes suspended solids and settling sludges caused by aerating pressure at the bottom of a lower pipe (i.e., Continuous Removal of Suspended solids and Settling sludges, CRSS). The CRSS plants are brought out by introducing fine air bubbles into the liquid phase of a lower pipe in the bio-reactor. These plant uses aeration pipe, with multiple inlets to sweep the floor of bio-reactor tank, instead of the conventional scraper mechanisms. The principal advantage of this system is that it can continuously remove very small or light particles that settles completely within a short time. Once the particles have been floated to the surface, they can be moved into the pipe and collected in the settling tank by sequently aerated pressure. The experimental results shows that about 99.0% of the biochemical oxygen demand(BOD), 99.3% of the suspended solid(SS), 92.3% of the total nitrogen(T-N), 99.0% of the turbidity(TU), 100% of the total coliform(TC)and ammonia was respectively removed during aerobic digestion for 9 days. These result indicates that the CRS S plants are very effective for reduction and deodorization of swine wastewater contaminants, and the efflux from CRS S can either be discharged in the river or used as nutrient solution of formulation for plant growth factories. The developed CRSS plant proved to be flexible and it can simply be adapted to any type of biological waste treatment problem.roblem.

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Analysis of Water Quality and Soil Environment in Paddy Fields Partially Irrigated with Untreated Wastewater (미처리하수지구 논에서의 수질 및 토양 특성 분석)

  • Song, Jung Hun;Jeong, Han Seok;Park, Ji Hoon;Song, In Hong;Kang, Moon Seong;Park, Seung Woo
    • Journal of The Korean Society of Agricultural Engineers
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    • v.56 no.6
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    • pp.19-29
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    • 2014
  • The objectives of this study were to monitor and analyse water quality and soil property in paddy fields where untreated wastewater is irrigated. Three paddy fields where streamflow mixed with untreated wastewater has been irrigated (untreated wastewater district, UWD) were selected for monitoring, and five paddy fields in Yongin area (Yongin district, YID) where water from Idong agricultural reservoir (well-managed) has been irrigated were selected for comparative evaluation. Electronic conductivity (EC), suspended solids (SS), total nitrogen (T-N), total phosphorous (T-P), $NO_3-N$, $Ca^{2+}$, $Mg^{2+}$, $Na^+$, total coliform (TC), fecal coliform (FC), and E. coli of the irrigation water in the UWD were significantly higher than those in the YID. Relatively high concentrations of EC, T-N, T-P, TC, FC, E. coli, copper (Cu), lead (Pb), zinc (Zn), and aluminium (Al) were shown in the irrigation water of the UWD especially during May to June. In general, the paddy soil in the UWD contained more Pb, Zn, and Cu than in the YID although the soil heavy metal contents in the UWD still meet the Korean soil contamination warning standards. No temporal trends in the heavy metal concentrations were found in paddy soils of the UWD. This study showed that the use of untreated wastewater to paddy fields has the possibility of negative impacts on water quality and soil, although long-term monitoring is needed to fully evaluate its effects.

Assessment of the Bacteriological Safety of Seawater and Oysters Crassostrea gigas in Yongnam-Gwangdo Area, Korea 2014-2016 (2014-2016년 경남 용남·광도해역 해수 및 굴(Crassostrea gigas)의 세균학적 위생평가)

  • Yoon, Hyun Jin;Kwon, Ji Young;Lee, Ka Jeong;Kwon, Soon Jae;Mok, Jong Soo;Kim, Poong Ho;Jung, Yeoun Joong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.51 no.5
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    • pp.535-541
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    • 2018
  • This study evaluated the sanitary state of seawater and shellfish in Yongnam-Gwangdo area from January 2014 to December 2016. The sampling stations for sanitary survey in Yongnam-Gwangdo area were composed of 41 seawater stations and 5 oyster Crassostrea gigas stations. The samples were collected monthly at each station. Shellfish-borne disease is associated with bacteria and viruses in the presence of fecal coliforms. Bacteriological pollution levels of shellfish increase with seawater quality. Therefore, fecal coliforms are very important criteria for evaluating the safety of fisheries in coastal areas. The geometric mean and the estimated 90th percentile ranges of total and fecal coliforms for seawater were 1.8-11.7, 2.8-233.6, <1.8-6.7 and 1.8-100.2 MPN/100 mL, respectively. The range of E. coli levels for oysters was <20-1,300 MPN/100 g. Based on various standards, the sanitary condition in Yongnam-Gwangdo area was evaluated as follows: clean area (Korean criteria), conditionally approved area (US criteria) and class B (EU criteria).

Anti-microbial Effects of Washing and Chlorine Treatments on Fresh Fruits (과일류의 염소 소독 농도 및 세척 횟수에 따른 미생물 제거 효과)

  • Park, Jong-Sook;Nam, Eun-Sook;Park, Shin-In
    • The Korean Journal of Food And Nutrition
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    • v.21 no.2
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    • pp.176-183
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    • 2008
  • This study examined the anti-microbiological effects of chlorine treatment on the surface of fresh fruits, in order to improve microbiological safety in school foodservice operations. Non-peeled fruit(strawberries) and peeled fruit(bananas) were treated with different concentrations of chlorinated water and rinsing numbers, followed by microbiological testing. The fruits were immersed at different concentrations of chlorinated water(0 ppm, 50 ppm, and 100 ppm) and durations(3 min and 5 min), and were then rinsed with tap water(one time, two times, or three times). The total viable cell counts of both the strawberries and bananas ranged from $10^3$ CFU/g to $10^4$ CFU/g, and coliform levels ranged from $10^2$ CFU/g to $10^3$ CFU/g. As the chlorine concentration, immersion time, and rinsing number increased, anti-microbiological activity increased. The largest microbial reduction was shown with immersion for 5 min in 100 ppm chlorinated water and three rinsings. In the strawberries, this treatment reduced the initial population of total viable cells and coliforms by 3.29 log CFU/g and to an undetectable level, respectively, no total viable cells or coliforms were detected on the banana surface following this treatment. However, after a sterilization treatment with immersion for 5 min in 50 ppm chlorinated water and three rinsings, the total viable cell counts and coliform counts of the strawberries and bananas decreased to acceptable levels, based on the microbiological standards for ready-to-eat foods. Overall, it was shown that the sterilization treatment of 50 ppm chlorinated water, soaking for 5 min, and three rinsings provided an effective reduction in surface microbes, and enhanced the microbiological safety of the fruit.

Assessment of the Microbial Level for Livestock Products in Retail Meat Shops Implementing HACCP System

  • Kim, Jung-hyun;Yim, Dong-Gyun
    • Food Science of Animal Resources
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    • v.36 no.5
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    • pp.594-600
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    • 2016
  • This study aimed to examine the microbial contamination levels in livestock products at retail stores. Beef, pork, and chicken samples from raw materials and final products were obtained between January and December 2015. All homogenized meat samples (25 g) were tested for the aerobic plate count (APC), coliform count (CC), and Escherichia coli count (E. coli). The highest APCs in meat samples, by month, at retail shops were obtained in September, followed by July, May, and October (p<0.001). However, APC was the highest in summer and the lowest in winter (p<0.001). Average APCs for beef, pork, and chicken samples were 2.90, 3.19, and 3.79 Log CFU/g, respectively (p<0.05). A comparison between different months revealed that, CC levels in meat samples ranged from 0 to 1.13 CFU/g, and the highest CC was obtained in August (p<0.001). By season, the highest CC was found in the summer, followed by autumn, and spring (p<0.001). All meat samples were negative for E. coli. The average log10APC and CC for all samples was 3.10 and 0.37 Log CFU/g, respectively. Furthermore, there was a direct correlation between the season and coliform presence (p<0.001). There was also a positive correlation between the APC and CC (r = 0.517, p<0.001). The microbiological APCs for livestock products were in most cases below 106 CFU/g.

Presence and Control of Coliform Bacteria in Kimchi (김치 발효중 대장균군의 소장과 억제에 관한 연구)

  • Chung, Chang-Ho;Kim, Youn-Soon;Yoo, Yang-Ja;Kyung, Kyu-Hang
    • Korean Journal of Food Science and Technology
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    • v.29 no.5
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    • pp.999-1005
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    • 1997
  • The consistant appearance of coliforms in fermenting kimchi was examined and measures of removing coliforms early in the fermentation were investigated. Allyl isothiocyanate $({\geq}50\;ppm)$, horseradish powder $({\geq}0.4%)$, and garlic juice $({\geq}2.0%)$ were effective in removal of coliforms early in kimchi fermentation. However, mustard powder and methyl methanethiosulfonate were not effective. Nisin, known as a promising agent for the prevention of kimchi over-acidification, allowed coliforms to survive in kimchi longer with only marginal extention of edible period. Individual kimchi ingredients such as Chinese cabbage, garlic, red pepper powder, ginger and green onion were all found to contain coliforms. Coliforms were not detected from garlics sold unpeeled and commercially prepared red pepper powder.

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Growth Characteristics of Microorganism on Lapilli-Cement mixed Bioblocks (화산력-시멘트 혼합 바이오 블록의 미생물 생장 특성)

  • Park, Sung-Yong;Park, Duck-Hwan;Kim, Hyun-Sun;Kim, Jung-Myeon;Lim, Hyun-Taek;Bae, Su-Bin;Kim, Yong-Seong
    • Journal of The Korean Society of Agricultural Engineers
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    • v.57 no.1
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    • pp.111-118
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    • 2015
  • This research aims to utilize lapilli from the Mt. Baekdusan as environmently-friendly construction material. First of all, the neutralizing method for fabricating lapilli-cement-mixed bioblock was examined. And then, by use of the neuralized bioblock with microorganism for water purification, the growth inhibition effect against the pathogenic coliform bacillus was evaluated. The result regarding growth inhibition effect on pathogenic coliform bacillus indicates that the pretreatment condition, which is a concurrent procession with aqueous solution of 10% di-ammonium Phosphate after water curing, led to pH degraded below 10 which was the target value. Therefore it was concluded that the method was effective on bio-block neutralization. The microorganisms purifying water and di-ammonium phosphate were detected through the examination for microorganism existence on the bioblock, therefore it was concluded that the bioblock composed of lapilli and cement is able to be utilized in various structures as an environment friendly construction material.

Evaluation of the Effect of the Discharged Water from Bong Stream after Rainfall Events on the Bacteriological Water Quality in Gangjinman, Korea (강우 발생에 따른 남해군 봉천 방출수가 강진만 해역의 세균학적 수질에 미치는 영향 평가)

  • Park, Kun-Ba-Wui;Jo, Mi-Ra;Lee, Hee-Jung;Kwon, Ji-Young;Son, Kwang-Tae;Lee, Tae-Seek
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.44 no.6
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    • pp.622-629
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    • 2011
  • We investigated the effect of the discharged water from Bong Stream, which is located in the drainage area of Gangjinman area on the bacteriological water quality in the coastal area after rainfall events. Following 12.5 mm of rainfall, water discharged from Bong Stream had a very limited effect on bacteriological water quality in adjacent area and the affected area did not extend to the designated area. On the other hand, after 23 mm rainfall, the density of fecal coliform at stations located in the designated area was higher than at stations located in the adjacent area. The degree of bacteriological contamination at the surveyed stations in the coastal area after rainfall events did not show a relationship with distance from the shoreline. These results indicate that the direction of spread and the range of contaminants from the drainage area were affected by tides at the time of the survey. Therefore, a detailed survey of the effects of tides on the diffusion characteristics of the contaminants from Bong Stream is needed to establish a proper management plan for the surveyed area.

Effect of Physicochemical Properties of Cured Loin Ham by Inoculation of Lactobacillus plantarum(II) (Lactobacillus Plantarum이 염지 햄의 이화학적 특성에 미치는 영향(II))

  • Chung, Yung-Gun;Hyun, In-Hwan;Kim, Jong-Kyu
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.15 no.1
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    • pp.69-74
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    • 1986
  • In order to examine the effect of 'Lactobacillus plantarum' inoculation on the maturation of cured loin ham, bacteriological and biochemical changes in meat were investigated during curing periods. The results were summarized as follow; On the bacteriological changes of cured ham, during curing periods, the number of coliform group were decreased. while psychrotrophic and halo-tolerant bacteria were increased until the 4 days. In the brine solution after the 7days of the curing, the number of coliform group were decreased the 7 days, but psychrotophic and halo-tolerant bacteria were increased until the 7 days of curing. The pH value of the meat and curing solution were sharply decreased at the one day, since these were slightly increased from 4 days. The color development of cured meat was showed 84.05 % development of within the 7 days of curing. Glutamic acid contents among the 17 kinds of amino acid were the highest at the 7 and 10 days of curing. The 13 kinds of fatty acids detected from at the all sample and total contents of unsaturated fatty acid were slightly decreased during curing.

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