• 동의생리병리학회지
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• 제22권6호
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• pp.1354-1358
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• 2008

In Traditional Korean Medicine it is one of basic principle that personality reflects physical conditions. In both of personality and ordinary symptoms, there are some criteria used for diagnosis whether the patient is in cold or hot condition. We studied the relationship between the criteria of cold-hot shown in personality pattern and ordinary symptom pattern. And also analyzed the relativeness in four constitution type. 877 patients who had visited oriental medical hospital and had been confirmed their constitution by drug response were included in this study. Patients filled out the questionnaire composed of personality and ordinary symptom questions. We selected questions related to cold-hot patterns. Next we made personality cold-hot index(PCHI) and ordinary symptom cold-hot index(OSCHI). The two indices were compared and analyzed totally and from the viewpoint of 4 constitution types. The coefficient of correlation between two indices was not high. In Soemin the coefficient was 0.257, in three other constitution it was lower than 0.200 or negative. In Soemin both indices were lower (cold-biased), but in three other constitution no significant results were found. In Tae-emin OSCHI was higher (hot-biased) than PCHI, in Tae-yangin PCHI was higher than OSCHI significantly. Personality is not affected by cold-hot less than expected. The criteria used for diagnosis of cold-hot seems to be biased on Soemin's.

• KSBB Journal
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• 제16권6호
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• pp.626-631
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• 2001

본 연구에서 L. crispatus KLB46을 저온 전처리함으로서 제제화 과정에 받게 되는 얼림과 녹임, 건조 스트레스뿐만 아니라, 여러 다른 환경 스트레스에 대한 내성이 증진된다는 것을 확인하였다. 그리고 내성 증진에 신규 단백질 합성이 필요함을 확인하였으며 나아가, 저온 충격 유전자 (csp)를 확인하였다. 따라서 이 균주를 제제화 하기 위한 방법으로 저온 전처리를 이용할 경우 생균력 유지에 큰 효과를 얻을 수 있다고 사료된다.

• 한국환경농학회지
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• 제27권4호
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• pp.389-394
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• 2008

The ecophysiological changes occurring upon cold stress were studied using cold tolerant transgenic and wild-type tobacco plants. In a previous study, cold tolerance in tobacco was induced by the introduction of a gene encoding the zinc finger transcription factor, PIF1. Gas-exchange measurements including net photosynthesis and stomatal conductance were performed prior to, in the middle of, and after a cold-stress treatment of $1{\pm}2^{\circ}C$ for 96 h in each of the four seasons. In both transgenic and wild-type plants, gas-exchange parameters were severely decreased in the middle of the cold treatment, but had recovered after 2-3 h of adaptation in a greenhouse. Most t-test comparisons on gas-exchange measurements between the two plant types did not show statistical significance. Wild-type plants had slightly more water-soaked damage on the leaves than the transgenic plants. A light-response curve did not show any differences between the two plant types. However, the curve for assimilation-internal $CO_2$ in wild-type plants showed a much higher slope than that of the PIF1 transgenic plants. This means that the wild-type plant is more capable of regenerating Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and has greater electron transport capacity. In conclusion, cold-resistant transgenic tobacco plants demonstrated a better recovery of net photosynthesis and stomatal conductance after cold-stress treatment compared to wild-type plants, but the ecophysiological recoveries of the transgenic plants were not statistically significant.

• Journal of Ginseng Research
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• 제44권5호
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• pp.747-755
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• 2020

Background: Ginsenosides accumulation responses to temperature are critical to quality formation in cold-dependent American ginseng. However, the studies on cold requirement mechanism relevant to ginsenosides have been limited in this species. Methods: Two experiments were carried out: one was a multivariate linear regression analysis between the ginsenosides accumulation and the environmental conditions of American ginseng from different sites of China and the other was a synchronous determination of ginsenosides accumulation, overall DNA methylation, and relative gene expression in different tissues during different developmental stages of American ginseng after experiencing different cold exposure duration treatments. Results: Results showed that the variation of the contents as well as the yields of total and individual ginsenosides Rg1, Re, and Rb1 in the roots were closely associated with environmental temperature conditions which implied that the cold environment plays a decisive role in the ginsenoside accumulation of American ginseng. Further results showed that there is a cyclically reversible dynamism between methylation and demethylation of DNA in the perennial American ginseng in response to temperature seasonality. And sufficient cold exposure duration in winter caused sufficient DNA demethylation in tender leaves in early spring and then accompanied the high expression of flowering gene PqFT in flowering stages and ginsenosides biosynthesis gene PqDDS in green berry stages successively, and finally, maximum ginsenosides accumulation occurred in the roots of American ginseng. Conclusion: We, therefore, hypothesized that cold-induced DNA methylation changes might regulate relative gene expression involving both plant development and plant secondary metabolites in such cold-dependent perennial plant species.

• 한국정밀공학회지
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• 제22권1호
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• pp.167-174
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• 2005

The design for cold extrusion dies is very important, because the die insert is subjected to very high radial and hoop stresses. The design of cold extrusion dies has many constrained conditions. In this paper, the used assumptions are such that the yield strength of each ring is selected according to the allowable tensile or compressive hoop stress in each ring and the maximum allowable inner pressure, when yielding occurs in one ring of the dies, is obtained by the proposed equation. In order to obtain design variables, such as diameter ratios and interferences, using the maximum inner pressure, the flexible tolerance method was used for shrink-fitted thick-walled cylinders. ANSYS APDL was used to perform the repeated analysis of deformation of the dies due to the variation of the design variables. The response surface methodology is utilized to analyze the relationship between the design variables and the maximum radial displacement of the die insert during extrusion. From the results, it is found that outer diameter of the die insert has the largest effect on the minimization of maximum radial displacement at the inner surface of the dies.

• 소성∙가공
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• 제15권8호
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• pp.550-555
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• 2006

In this study, effects of forming angle and friction coefficient on a initiation of the Mannesmann hole defect were analyzed by using a response surface method. The maximum effective plastic strain at center point of specimen is utilized for the prediction of the starting point of crack occurrence, which is suggested by the comparison of integrals of four different ductile fracture models between the histories of the effective plastic strain at center point. It was revealed that the principal stress at the center is the dominant element to the increase of the effective plastic strain. It was also verified by the simulation results from the comparison of experiment and simulation. It is provided that the forming angle of 25 degrees and the spreading angle of 1 degree can be a proper design condition without an occurrence of internal hole defect and an excessive slip.

• The Korean Journal of Physiology and Pharmacology
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• 제3권4호
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• pp.365-373
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• 1999

Somatostatin (SOM) is one of the major neuropeptides in dorsal root ganglion cells, but its role in spinal nociceptive process has not been well known. In present study we aimed to investigate the effect of SOM on the response of dorsal horn neurons to the various types of peripheral nociceptive stimuli in anesthetized cats. Using carbon-filament microelectrode, the single cell activities of wide dynamic range neurons were recorded from the lumbosacral enlargement after noxious mechanical (squeeze), thermal (radiant heat lamp) and cold (dry ice) stimulation to the receptive field. Sciatic nerve was stimulated electrically to evoke $A\;{\delta}-$ and C-nociceptive responses. SOM analogue, octreotide $(10\;{\mu}g/kg),$ was applied intravenously and the results were compared with those of morphine (2 mg/kg, MOR). Systemic SOM decreased the cellular responses to the noxious heat and the mechanical stimulation, but increased those to the cold stimulation. In the responses to the electric stimuli of sciatic nerve, $A\;{\delta}-nociceptive$ response was increased by SOM, while C-nociceptive response was decreased. On the other hand, MOR inhibited the dorsal horn cell responses to all the noxious stimuli. From the above results, it is concluded that SOM suppresses the transmission of nociceptive heat and mechanical stimuli, especially via C-fiber, while it facilitates those of nociceptive cold stimuli via $A\;{\delta}-fiber$.

• Journal of Acupuncture Research
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• 제15권2호
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• pp.157-171
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• 1998

In order to study the effect of direct moxibustion on depressed immune response and thyroid hormone in rats exposed to cold stress, Sprague-Dawley male rats were put in the horizontal refrigerator by $-10^{\circ}C$ for 14 days and thereafter $-18^{\circ}C$ for 11 days(control group). Sample I group was treated by daily direct moxibustion to bilateral Shinsu(BL23) from the 22nd day to the 25th day for 4 days under the same condition with the control group. Sample II group was treated by daily direct moxibustion to bilateral non-acupoints near the root of the tail from the 22nd day to the 25th day for 4 days under the same condition with the control group. And RBC, WBC, CD4+ T cell count, T3, T4 and TSH were measured. The results were as follows; 1. RBC increased with statistical significance in the sample I and sample II groups compared with the control group. 2. WBC increased with statistical significance in the sample I group, but there was not any significance in the sample II group compared with the control group. 3. Lymphocyte increased with statistical significance both in the sample I and sample II groups compared with the control group. 4. CD4+ T cell count increased with statistical significance both in the sample I and sample II groups compared with the control group. 5. T3, T4 and TSH increased respectively with statistical significance in the sample I and sample II groups compared with the control group.

• 한국화재소방학회논문지
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• 제30권5호
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• pp.26-35
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• 2016

스프링클러 설비는 화재 발생 시 화재를 초기에 제어 및 진압하는 설비로서 어떠한 화재 분위기에서도 스프링클러 헤드의 작동온도에서 정상적인 동작을 하여야 한다. 그러나 최근 화재성장속도가 느린 화재 발생 장소에서 일부 조기반응형 스프링클러 헤드가 정상적인 동작을 하지 않을 수 있다는 문제가 제기되고 있다. 이에 본 논문에서는 공동주택 침실규모의 모형실험 및 캐비넷형 버너를 이용하여 화재성장속도가 느린 조건을 형성하고 실제 현장에서 배관 내에 가해지는 최소압력인 0.2MPa 이상의 가압수를 공급하여 실험을 진행하였다. 실험결과 국내에서 사용되고 있는 과반수 이상의 플러쉬형 조기반응형 스프링클러 헤드에서 Cold Soldering 현상이 발생되면서 불완전하게 개방되는 것이 확인되었다. 이러한 현상은 화재 조기진압 및 제어에 실패하여 화재확산 및 인명피해의 원인이 될 수 있으므로 추가적인 연구를 통해 국내 형식승인 및 제품검사기준의 보완의 필요할 것으로 판단된다.

• 원예과학기술지
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• 제34권1호
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• pp.46-54
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• 2016

2-Oxoglutarate (2OG) acts as a signaling molecule and plays a critical role in secondary metabolism in a variety of organisms, including plants. Six 2-oxoglutarate (2OG) and Fe(II) oxygenase (2OGO) genes, VlCE2OGO1 [Vitis labruscana 2-oxoglutarate (2OG) and Fe(II) oxygenase 1], VlCE2OGO2, VlCE2OGO3, VlCE2OGO4, VlCE2OGO5, and VlCE2OGO6, which show different expression patterns upon transcriptome analysis of 'Campbell Early' grapevine exposed to low temperature for 4 weeks, were analyzed for their structure and expression. Comparison of the deduced amino acid sequences of the 2OGO genes from the V. labruscana transcripts revealed sequence similarities of 38.6% (VlCE2OGO1 and VlCE2OGO2) to 19.2% (VlCE2OGO2 and VlCE2OGO3). The lengths of these genes ranged from 1053 to 2298 bp, and they encoded 316 to 380 amino acids. The prediction of the secondary structure of the encoded proteins by Self-Optimized Prediction Method with Alignment (SOPMA) indicated that all the genes contained alpha helix (23.95 to 41.71%), extended strand (16 to 22.34%), beta turn (6.65 to 9.22%), and random coil (32.97 to 51.58%) in the analysis. Specific primers from unique regions in each gene obtained by alignment of nucleotide sequences were used in real time PCR for analysis of gene expression. All tested genes showed differential expression in grapevines exposed to low temperature. Of the six transcripts, VlCE2OGO1, VlCE2OGO2, and VlCE2OGO3 were up-regulated and VlCE2OGO4, VlCE2OGO5, and VlCE2OGO6 were down-regulated in response to cold treatments at all tested time points. The 2OG genes can be used for elucidation of mechanisms of tolerance to cold and as valuable molecular genetic resources for selection in breeding programs for cold-hardy grapevines.