• Journal of Applied Biological Chemistry
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• 제60권3호
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• pp.257-263
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• 2017

A cellulolytic strain Y2 was isolated from soil obtained in the Canadian Alpine region. The isolate was identified as Paenibacillus sp. Y2 by 16S rRNA sequencing. When grown in LB medium supplemented with carboxymethyl-cellulose (CMC), CMCase production increased to 122.0% of that observed in LB without CMC. Culture supernatant was concentrated by ultrafiltration and 80% ammonium sulfate precipitates were separated by Hi-Trap Q and CHT-II chromatography. The purified enzyme (EG-PY2) showed a homogeneous single band and the molecular mass was estimated to be 38 kDa by SDS-PAGE. Optimum pH and temperature of the enzyme were 4.5 and $30^{\circ}C$, respectively. The half-life of enzyme activity at 50 was 140.7 min, but the enzyme was drastically inactivated within 5 min at $55^{\circ}C$. The enzyme was highly activated to 135.7 and 126.7% by 5.0 mM of $Cu^{2+}$ or $Mg^{2+}$ ions, respectively, and moderately activated by $Ba^{2+}$ and $Ca^{2+}$ ions, whereas it was inhibited to 76.8% by $Fe^{2+}$, and to ${\leq}50%$ by $Mn^{2+}$, $Co^{2+}$, $Zn^{2+}$, and EDTA. The enzyme was activated to 211.5% in the presence of 0.5 M of NaCl and greatly tolerant to 3.15M of NaCl. The enzyme showed 2.98 times higher ${\beta}$-glucanase activity than CMCase activity. Based on these results, it can be concluded that EG-PY2 is an acidophilic, cold-active, and halotolerant endoglucanase. The authors suggest it is considered to be useful for various industrial applications, such as, fruit juice clarification, acidic deinking processes, high-salt food processing, textile and pulp industries, and for biofuel production from seaweeds.

• Asian Pacific Journal of Cancer Prevention
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• 제17권7호
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• pp.3317-3320
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• 2016

Purpose: To assess the success rates of two step loop electrosurgical excision procedure (LEEP) compared with conventional cold conization procedures for decreasing positive surgical margins. Materials and Methods: This study was conducted on 70 patients who underwent colposcopic evaluation in Zeynep Kamil Women and Children's Health Training and Research Hospital between 2013-2015 with indications of CIN 2/3 or persistent CIN 1 for more than 2 years. The study included age matched groups of patients with similar histopathololical lesions who underwent cold conization (n=40) or LEEP (N=30). Results: Comparison of tissue characteristics between the two groups revealed significantly higher deepest depth and lower volume of tissue removed by the two step LEEP. Ectocervical positivity rate was similar between groups (1/39 versus 0/29, P>0.05), while endocervical surgical margin positivity rate was significantly higher in the cold conization group (9/39 versus 0/29, P<0.05). Surgical margin positive cases were significantly older than the cases with negative margins (P<0.05). Conclusions: Two step LEEP made it easier to reach the squamocolumnar junction in the endocervical region with lower blood loss and applicability in office settings. Our study suggests to use two step approach in cases with high grade and glandular CIN.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2004년도 추계학술대회논문집
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• pp.319-324
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• 2004

Cross wedge rolling process is utilized to manufacture multi-stepped axis symmetrical parts. This process is generally performed under high temperature conditions in order to induce serious deformation. But cold cross wedge rolling process has been rarely studied due to the limits of deformation. Recently, the cold cross wedge rolling process has been utilized to enhance the material strength in specified parts of manufactured products. In this paper, experimental researches were carried out with various forming conditions of cold cross wedge rolling process in order to suggest the design guidance to make preform for cold cross wedge rolling. The tensile strength and the surface hardness of specified region were compared to that of initial material with the variation of the area reduction and the rotational speed of rolling die. With respect to the area reduction, the maximum tensile strength was linearly increased and the surface hardness was rapidly increased within lower percent of area reduction. The surface hardness was saturated over the rotational die speed of 0.8 RPM.

• 소성∙가공
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• 제17권4호
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• pp.272-277
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• 2008

A constitutive equation of the electrical steel strip used for a raw material of transformer is proposed. The stress-strain behavior of electrical steel strip is quite different from that of common carbon steel and/or alloy steel. A series of tensile tests were performed with the specimens made from cold rolled strip. Several thicknesses of the strip were produced by a two-high (with upper and lower rolls) cold rolling pilot mill as reduction ratio increases from 10% to 90%. Its initial thickness of the strip was 2.5mm. Tensile specimens are cut out from the cold rolled strips. Mechanical properties of the steel are examined through rolling direction. Ramberg-Osgood model and the proposed equation are combined to describe the total behavior of stress-strain including instability region. The stress-strain curves calculated from the present constitutive equation are compared with those from experimentally obtained at each test condition of reduction ratios of specimen. Results show that the predicted stress-strain curves are in overall in a good agreement with measured ones.

• 태양에너지
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• 제20권1호
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• pp.97-108
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• 2000

The purpose of this study is to minimize the heat transfer surface area and cold fluid exit temperature of heat exchanger which applied to the refrigeration and air-conditioning system by utilizing the thermoelectric principle. Both uniform and non-uniform current distribution methods which applied to the analysis of the TE elements that incorporates heat exchanger were investigated. The non-uniform current distribution method had the better coefficient of performance and had the lower cold fluid exit temperature of the TE cooling system than the uniform current distribution method. It was found that if a TE cooling system incorporates a heat exchanger, a non-uniform current distribution should guarantee to the lowest cold fluid exit temperature. Also, the hybrid method (combination of the uniform and non-uniform current distribution method) is investigated to achieve the best results by combining the uniform and non-uniform current distributions. The results show that it can lower the cold fluid exit temperature and reduce the heat transfer surface area for the parallel flow arrangement if we apply the constant current in some entry region and the non-uniform increasing current in the direction of the cold fluid flow afterwards.

• 한국유기농업학회지
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• 제21권4호
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• pp.711-723
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• 2013

지난 25년간 지구온난화로 인한 국내 기후변화 양상은 지역 간에 차이가 있어, 고온지역은 평균온도의 증가를 보이지는 않았으나, 최저기온의 지속적인 상승과 그에 따른 열대야 발생을 확인할 수 있었다. 또한 그로 인한 젖소의 고온스트레스 발생, 섭취량의 감소 및 생산성 저하가 예상된다. 저온지역의 경우에는 여름철 평균온도 및 최저온도의 상승으로 인해 연평균기온이 유의적으로 상승한 반면, 겨울철에는 오히려 최저기온의 지속적인 하강이 관찰됨에 따라 동물의 저온스트레스가 가중될 것으로 예상된다. 착유우가 저온스트레스 상태에 있을 때는 에너지요구량과 건물섭취량이 증가하여 생산 효율이 떨어지며, 사료효율의 감소로 경제성은 감소되고 우유 생산비는 증가한다. 특히, 극심한 저온스트레스 또는 사료, 음수 및 우사바닥의 결빙은 섭취량 감소를 야기하며 이에 따른 생산성의 저하는 더욱 가중될 것으로 예상된다. 결론적으로 지구온난화로 인한 기후변화에 따른 지역별 영향은 다르며, 온도 스트레스에 의한 낙농우의 생산성 저하를 최소화하고, 동물의 복지와 건강을 증진시키기 위해서는 지역별 기후변화 특성에 맞춘 사양기술의 개발이 필요하다.

• 한국지반환경공학회 논문집
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• 제17권1호
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• pp.49-54
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• 2016

동토지반을 구성하는 지층 중 표층에 분포하는 활동층은 계절에 따라 동결과 융해를 반복하여 지표면의 동상을 야기한다. 동상 높이는 활동층의 두께에 큰 영향을 받으므로, 동토지반 상부 인프라시설의 안전한 설계 및 시공을 위하여 활동층의 두께 산정은 매우 중요한 부분을 차지한다. 본 연구에서는 경량화된 원위치 관입시험 방법인 동적 콘 관입기를 이용하여 동토지반에서 활동층이 분포하는 심도를 평가하고자 하였다. 동적 콘 관입시험을 적용하기 위한 대상현장으로서 알래스카에 위치한 솔로몬 지역의 동토지반이 선택되었으며, 해당 지역의 두 개소에서 지중온도계측 및 동적 콘 관입시험이 수행되었다. 적용실험 결과 동적 콘 관입시험으로부터 획득된 동적 콘 관입지수는 활동층 및 영구동토층에서 서로 상이한 값을 나타내는바 동적 관입특성에 따른 활동층과 영구동토층의 경계부 심도가 산정되었으며, 경계부 심도에서 아이스 렌즈층으로 판단되는 구간이 감지되었다. 해당 개소에서 획득된 지중온도분포도에서 영상 및 영하 온도의 경계 심도는 본 연구의 동적 콘 관입시험으로부터 획득된 활동층 분포 심도와 부합하는 결과를 보였다. 본 연구에서 적용된 동적 콘 관입기는 대형장비의 접근 및 적용에 한계가 있는 극한지 동토 지역의 활동층 심도평가를 위하여 활용될 수 있을 것이라 기대된다.

• Ocean and Polar Research
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• 제27권3호
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• pp.237-250
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• 2005

To investigate the air mass modification related with Yeongdong Heavy snowfall events, we examined sensible and latent heat fluxes on the East Sea, the energy exchange between atmosphere and ocean in this study. Sensible and latent heats were calculated by a bulk aerodynamic method, in which NCEP/NCAR reanalysis data and NOAA/AVHRR weekly SST data with high resolution were used. Among winter precipitation events in the Yeongdong region, 19 heavy precipitation events $(1995{\sim}2001)$ were selected and classified into three types (mountain, cold-coastal, and warm types). Mountain-type precipitation shows highly positive anomalies of sensible and latent heats over the southwestern part of the East Set When separating them into the two components due to variability of wind and temperature/ specific Humidity, it is shown that the wind components are dominant. Cold-coastal-type precipitation also shows strong positive anomalies of sensible and latent heats over the northern part and over the central-northern part of the East Sea, respectively. It is shown that the sensible heat anomalies are caused mostly by the decrease of surface air temperature. So it can be explained that cold-coastal-type precipitation is closely related with the air mass modification due to cold air advection over warm ocean surface. But in warm-type precipitation, negative anomalies are found in the sensible and latent heat distributions. From this result, it may be postulated that warm-type precipitation is affected by the internal process of the atmosphere rather than the atmosphere-ocean interaction.

• Plant Breeding and Biotechnology
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• 제1권1호
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• pp.1-8
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• 2013

Low-temperature stress is an important factor controlling the growth and development of rice (Oryza sativa L.) in temperate region. In this study, a molecular linkage map consisting of 136 SSR markers was employed to identify QTL associated with cold tolerance at the seedling stage. 80 recombinant inbred lines (RILs) from an intersubspecific cross between Milyang23 (O. sativa ssp. Indica) and Hapcheonaengmi3, a japonica weedy rice and the parents were evaluated for leaf discoloration and SAPD value of seedlings. Rice plants were grown for 15 days in the low-temperature condition (13/20℃ day/night) and the control condition (25/20℃ day/night) in the growth chamber. The degree of leaf discoloration showed a highly significant correlation with the SPAD value in the low-temperature plot (r = -0.708, P < 0.0001). A total of four QTLs for SPAD were identified and the phenotypic variance explained by each QTL ranged from 5.4 to 16.0%. Two QTLs detected in the control condition were located on chromosomes 2 and 5, respectively. Two QTL on chromosomes 1 and 4 were detected at the low-temperature condition and Hapcheonaengmi3 alleles increased the SPAD values at these loci. Substitution mapping was conducted to delimit the position of qSPA-4 using introgression lines derived from the same cross. Results indicated that qSPA-4 was located in a 810-Kb region flanked by RM16333 and RM16368. The results indicated that Hapcheonaengmi3 contains QTL alleles that are likely to improve cold tolerance of Indica rice.

• 한국지반신소재학회논문집
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• 제22권4호
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• pp.63-71
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• 2023

남·북극을 포함한 극한지는 풍부한 자원이 매장되어 있지만 접근성이 어렵고, 국내와 전혀 다른 건설 환경 때문에 자원개발 사업 추진에 많은 제약이 있다. 최근 지구온난화로 극한지의 접근성이 용이해짐에 따라 자원개발 플랜트 건설 수요가 증가하고 있다. 이러한 지역에서의 자원개발 건설 활동을 위해서는 사업추진의 리스크를 최소화할 수 있도록 사전 입지 분석이 필요하다. 본 논문에서는 극한지 건설 활동에서 고려해야 할 주요 요인들을 분석하여, 공간정보(GIS, Geographic Information System) 기반의 건설환경 플랫폼 및 최적 입지 선정 시스템을 새롭게 개발하였다. 오일샌드 개발이 활발하게 진행되고 있는 캐나다 앨버타주(Alberta) 대상으로 개발 시스템을 적용한 결과, 현재 운용되고 있는 오일샌드 플랜트 채굴 지역과 일치함을 확인하였다.