• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2003년도 학술발표대회 발표논문초록집
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• pp.80-80
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• 2003

Huntington's disease (HD) is an autosomal dominant neurodegenerative disorder characterized by motor, cognitive, and psychiatric symptoms, accompanied by marked cell death in the striatum and cortex. Stereotaxic injection of quinolinic acid (QA) into striatum results in a degeneration of GABAergic neurons and exhibits abnormal motor behaviors typical of the illness. The objective of this study was carried out to obtain basic information about whether parthenogenetic mouse embryonic stem (PmES) cells are suitable for cell replacement therapy of HD. To establish PmES cell lines, hybrid F1 (C57BL/6xCBA/N) mouse oocytes were treated with 7% ethanol for 5 min and cytochalasin-B for 4 hr to initiate spontaneous cleavage. Thus established PmES cells were induced to differentiate using bFGF (20ng/ml) followed by selection of neuronal precursor cells for 8 days in N2 medium. After selection, cells were expanded at the presence of bFGF (20 ng/ml) for another 6 days, then a final differentiation step in N2 medium for 7 days. To establish recipient animal models of HD, young adult mice (7 weeks age ICR mice) were lesioned unilaterally with a stereotaxic injection of QA (60 nM) into the striatum and the rotational behavior of the animals was tested using apomorphine (0.1mg/kg, IP) 7 days after the induction of lesion. Animals rotating more than 120 turns per hour were selected and the differentiated PmES cells (1$\times$10$^4$cells/ul) were implanted into striatum. Four weeks after the graft, immunohistochemical studies revealed the presence of cells reactive to anti-NeuN antibody. However, only a slight improvement of motor behavior was observed. By Nissl staining, cell mass resembling tumor was found at the graft site and near cortex which may explain the slight behavioral improvement. Detailed experiment on cell viability, differentiation and migration explanted in vivo is currently being studied.

• 인간식물환경학회지
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• 제22권3호
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• pp.269-277
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• 2019

This study aims to see what changes flower arrangement programs bring to the stress index of elderly people with chronic diseases and its correlation with cognition. Furthermore, seniors from a day care center and a nursing home were compared for the purpose of identifying the effectiveness of flower arrangement activities as a supplementary remedy designed to relieve the symptoms and improve the quality of life of patients with chronic diseases. In this study, 24 seniors with chronic diseases were divided into two groups: Group A consists of 15 seniors from a day care center and Group B consists of nine seniors from a nursing home. Both groups participated in simple flower arrangement activities in 10 sessions. In each session, red and green cut flowers that were preferred by the elderly were provided to elicit and develop their thoughts and behaviors. The results showed that the overall average stress index was significantly reduced from 58.0±11.6 before the program to 50.6±17.1 after the program (p = .037). This study examined that the program was more effective for Group A (p = .021) than for Group B (p = .678). Results of the correlation analysis showed that there was no correlation between cognitive function and stress index (p = .569), but that the activity was more effective for Group A. This study shows that arranging flowers effectively lowers the stress index of elderly people with chronic diseases. Also, sharing the outcomes of such activities with others or continuously caring for the flowers even after the activity can maximize the effectiveness of the therapy and rehabilitation. Thus, the study concluded that the program needs to be applied continuously, not in the short-term, in order to relieve or treat the symptoms of elderly patients with chronic diseases.

• 인간행동과 음악연구
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• 제17권2호
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• pp.1-28
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• 2020

본 연구는 그룹 음악심리치료에 참여한 게임 과몰입 청소년의 게임 이용동기 인식 변화를 탐구하고자 실시한 사례연구이다. 그룹 음악심리치료 프로그램은 회기당 60분씩 총 6회기 시행되었고, 연구 참여자는 게임 과몰입 청소년 5명이었다. 연구 자료는 세션에 참여하면서 나타난 참여자의 음악적·언어적 반응, 프로그램에서 작업한 가사와 세션 종결 후 사후 인터뷰를 통해 수집한 참여자의 언어 보고이다. 자료분석 결과 참여자들은 공감되는 가사에 대해 토의하면서 자신의 내재된 부적 정서와 관계적 어려움에 대해 직면할 수 있었고, 가사 창작 작업을 하면서 게임에 몰입했던 과거의 동기에 대해 인식할 수 있었다. 이와 같은 음악중재를 통해 참여자들은 자신의 게임 과몰입 행동에 대해 인식함과 동시에 게임 과몰입 행동 조절이 어려운 이유를 이해할 수 있었다. 또한, 이러한 자각이 게임 이용에 대한 인식 변화에 도움이 되었고, 실제 게임 이용 행동에 있어 변화가 있었다고 참여자들은 사후 인터뷰에서 보고하였다. 치료사는 치료회기에서 완성한 음악 창작물을 일상에서도 활용하도록 제공하였는데, 이로 인해 참여자들은 게임행동 조절이 용이했다고 보고했다. 연구 결과 그룹 음악심리치료 프로그램이 게임 과몰입 청소년의 게임 이용동기에 대한 인식을 가능하게 했고, 게임이용 행동 변화에도 긍정적 영향을 미쳤음을 시사한다.