• 식물조직배양학회지
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• 제27권6호
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• pp.491-496
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• 2000

더덕의 효과적인 재분화조건 (약 90%의 재분화율)을 바탕으로 개화시기 조절유전자인 RAG25 Codonopsis lanceolata유전자의 도입을 시도하였다. 더덕의 잎 절편을Agrobacterium과 3일 동안 공조배양 하였으며 NAA 2 mg/L, BA 2 mg/L (N2B2)와 NAA 2 mg/L, BA 4 mg/L (N2B4)가 첨가된 shoot inducing media (Km 20mg/L와 Cf 250 mg/L가 첨가됨)에서 배양하였을 때 형질전환 신초가 형성되었다. 획득된 신초에서 genomic DNA를 추출하고 npt II와 RAG25 primer를 이용한 PCR을 수행하여 각각 0.7 kb와 0.6 kb의 band를 확인하였다. RAG25 유전자에 대한 PCR산물을 이용하여 Southern hybridization을 수행한 결과 PCR과 동일한 위치에서 band 가 검출되어져 이 유전자의 도입을 재확인하였다.

• 한국조리학회지
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• 제20권4호
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• pp.183-192
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• 2014

본 연구는 더덕 잎의 기능성식품 및 화장품 소재로서의 이용가능성을 조사하기 따라서 더덕 잎의 영양성분 분석을 통한 식품영양학적 접근, 생리활성 기능을 기대할 수 있는 관련 물질 함량의 분석하였다. 식품영양학적 접근에서의 더덕 잎의 일반성분은 건량기준으로 탄수화물 69.12%, 조단백질 19.14%, 조지방 1.42% 및 조회분 10.32%이었고 더덕 잎 100 g의 함유 열량은 288.36 kcal로 분석되었다. 또한, 필수아미노산과 비필수아미노산 함량은 각각 5,906.41 mg, 7,551.56 mg이었고, 무기질 중 칼륨의 함유량이 가장 높았고 그 다음이 칼슘, 인, 마그네슘 순으로 나타나 알칼리성 재료임을 알 수 있었다. 유리당의 경우 sucrose의 함량이 전체 유리당의 약 42%를 차지하고 있는 것으로 나타났으며, 더덕 잎의 조사포닌 함량은 97.51 mg/g을 함유하고 있는 것으로 나타났다. 더덕 잎의 총 페놀 함량은 1.87 mg/g, 플라보노이드 함량은 0.61 mg/g으로 분석되었으며, DPPH 소거 활성을 농도별로 측정하여 비교한 결과 $1,000{\mu}g/mL$ 농도에서 64.32%의 활성을 보였다. 이상의 결과로부터 더덕 잎 추출물은 항산화능이 있음을 확인할 수 있었다. 따라서 더덕 잎을 식품 첨가물로서, 혹은 다른 약용으로의 조리 된 식품으로써의 활용가능성이 있을 것으로 판단된다.

• 한국식물병리학회지
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• 제14권6호
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• pp.676-681
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• 1998

Disease incidence of Codonopsis lanceolata was surveyed at the major cultivating fields in Chonbuk province in 1996 to 1997. The main diseases of Codonopsis lanceolata were ovserved as leaf spot caused by Septoria codonopsis, anthracnose by Glomerella cingulata, brown leaf spot by Cercospora sp., rust by Coleosporium koreanum, powdery mildew by Erysiphe sp., Fusarium wilt caused by Fusarium oxyporum, and white root rot by Sclerotium rolfsii. Anthracnose, leaf spot and brown leaf spot occurred severely on leaves from early July to late August. They were caused early fallen leaves. Fusarium wilt and white root rot occurred severely on stem and below the soil line in late August. They resulted in withering to death or chlorosis and fallen of leaves. Disease incidence of Codonopsis lanceolata was also substantially different in occurrence with a method of cultivation in late growth stage. Fusarium wilt and white root rot were more severe with a method of no support cultivation than those with a method of support cultivation with a stick. Fusarium wilt occurred 48.8% in a method of no support cultivation but 3.1% in a method of support cultivation with a stick. And white root rot occurred 18.9% in a method of no support cultivation but 0.3% in a method of no support cultivation with a stick. Thus, it proved that soil-borne diseases could be controlled support cultivation with a stick.

• 동아시아식생활학회지
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• 제22권6호
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• pp.860-867
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• 2012

Codonopsis lanceolata serves as, an appetizing health food for its, characteristic flavor, taste, and texture:however, it requires a long and complicated cooking process. Therefore, this study conducted pre-processing procedures to improve the cooking process and Codonopsis lanceolata more easily. For processing efficiency, freezing and blanching were efficient for peeling and the rate of peel disuse. In addition the freezing process, effectively reduced the time spent in beating raw material. In the salinity test, freezing and blanching quickly increased salinity over 28 days. As the result of SEM cross-section tests showed that frozen Codonopsis lanceolata become porous, it's assumed that seasoning permeates into this cross-section efficiently. As the appearance of Deodeok-gui as significant differences according to the pre-processing methods, this study examined pre-processing methods(time spent in peeling, rate of peel disuse, time spent in beating out material, salinity, moisture content, texture, and the color) of Codonopsis lanceolata. After testing Deodeok-gui, it was found that freezing is effective, not only for process efficiency and cooking time reduction, but also for improving its qualities.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 1996년도 더덕의 연구
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• pp.117-121
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• 1996

• 한국식품과학회지
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• 제24권2호
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• pp.171-176
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• 1992

더덕(Codonopsis lanceolata)의 휘발성 향기성분을 분석하기 위해 gas co-distillation(GCD), solvent extraction/fractionation(SLF), headspace sampling(HSS) 방법을 사용하여 향기성분을 분리농축하고 분석 비교한 결과 SEF와 HSS 방법으로 얻은 농축물들의 향이 원래의 더덕향과 가장 유사했으며 gas chromatogram들의 양상도 비슷했으나 peak들의 크기에 있어서 차이를 보였다. SEF 농축물에서는 달콤한 향을 기리고 HSS 농축물에서는 풋내를 비교적 강하게 느낄 수 있었으나 GCD 농축물의 경우 일정 증류시간이 지나면 한악취를 느낄 수 있었다. SEF와 HSS 방법은 GCD에 비해서 적은 조작 단계로 손쉽게 빠른 시간내에 분리 농축이 가능했다. 농축한 향기성분은 GC-MS와 극성이 다른 두 column을 사용한 GC-RI system을 이용하여 확인하였다. 특히 GC-RI는 isomer의 확인에 효과적이었으며 극성 FFAP column은 극성 화합물의 확인에 보다 적합했다. 35가지의 향기성분들을 동정하였는데 그중 24개의 성분들은 simultaneous distillation extraction 농축물에서는$^{(5)}$ 확인되지 않았던 향기성분들이다. 화인된 성분들중 trans-2-hexenal, cis-3-hexen-1-ol, trans-2-hexen-1-ol, hexanol은 더덕의 풋내를, 1-octen-3-ol은 신선한 풍미를 그리고 amyl propionate를 비롯한 다수의 에스터 화합물 등은 더덕 특유의 달콤한 향에 복합적으로 작용할 것으로 추정된다.

• 한국식품영양학회지
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• 제34권3호
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• pp.295-301
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• 2021

Steaming is a method that has traditionally been used for medicinal plant extraction. This study investigated nitrite oxide production, ferrous ion chelating activity, α-glucosidase, xanthine oxidase, and acetylcholinesterase inhibitory activities of ethanol, acetone and hot-water extracts of Codonopsis lanceolata prepared by steaming seven times. MTT assay showed that each extract was non-toxic up to a concentration of 700 ㎍/mL confirming that there was no cytotoxicity in all extracts. The α-glucosidase, xanthine oxidase, and acetylcholinesterase inhibitory activities exhibited by the hot-water extract obtained from steaming seven times were higher (83.1%) than the other extracts. Higher production of nitrite oxide and better ferrous chelating activity was recorded with hot-water extract compared to ethanol and acetone extracts. These results indicated that more steaming of Codonopsis lanceolata extracts would be required to validate the possibility of developing antioxidants. Also, further study is needed to determine if the components present in the tested extracts might be useful in the prevention of Alzheimer's disease. These results showed that hot-water extracts may be useful for their antioxidant and the production inhibitory activity of nitrite oxide. It will be helpful in the investigation of the constituent analysis of the steam-processed product of Codonopsis lanceolata.

• 한국자원식물학회지
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• 제5권1호
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• pp.31-35
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• 1992

The somatic chromosome numbers were counted to be 2n = 16 of Codonopsis lanceolata. Chromosomes 2 and 7 of Sokrisan and Koheung II has a hetero satellite on short arm. Chromosome 5 of Bakwoonsan showed homo satllite on short arms. Short arms of chromosomes 1 and 5 has homo satellite and short arms of chromosomes 2 and 7 has hetero satellite of Jilin.

• 한국자원식물학회지
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• 제4권2호
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• pp.47-49
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• 1991

In an attempt to analyse the variation of chromosome numbers in Cododopsis lanceolate, 28 species of Deo-Deong have been examined for determination of chromosomenumbers. Tlle somatic chromosone numbers were counted to be 2n=16 of C. lanceolata.

• 한국약용작물학회지
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• 제13권5호
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• pp.293-297
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• 2005

A thoredoxin (CTRX) gene was cloned and characterized from a full length cDNA library prepared from taproot of three-year old Codonopsis lanceolata. A CTRX was 666 nucleotides long and has an open reading frame of 372 bp with 124 amino acid residues (pI = 4.92). The deduced amino acid sequence of the CTRX matched to the previously reported plant thioredoxin h genes. The deduced amino acid sequence of CTRX exhibited the similarity of 33-67% among previously registered thioredoxin genes. The expression of CTRX in leaves of Codonopsis lanceolata was increased by wounding and 1 mM $H_2O_2$, but decreased by 0.1 mM cadmium.