• The Korean Journal of Food And Nutrition
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• v.14 no.6
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• pp.548-561
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• 2001

Listeria spp. from sea water, fishes and shellfishes have been troubled in many countries. So we exam ined its distribution rates, biochemical characteristics of a separated strain, growth curve of pH at set times to 4 species of standard strain, and yes or no of growth inhibition for precautionary measure of food poisoning by L. monocytogenes, garlic, mustard, wasabi, and green tea extracts including sensitivity of antibiotics 10 species. As its results, check numbers of its positivity to Listeria spp. were 32 species in total examination body 200 species, and its isolation rates were 16%, L. innocua was 14.0%, L. monocytogenes 1.0%, and L. seeligeri 1.0% by the strain species. All the standard strain of 4 species showed growth inhibition bellow pH 3.0, its pH conditions of the optimum growth at 7.0∼8.0, and its growth was more active in alkali co]tuition than in acid condition. Its growth inhibition examination by garlic extracts had an the worst effects with O.D values of 0.078∼0.210. But the case of mustard and wasabi had weakened effect, and the case of green tea had some effect as the time went by. The results of sensitivity examination of antibiotics 10 species were as fellows. L. innocua of the 16 cases showed sensitivity of 100% in all 5 species, Ampicillin, etc, and Ciprofloxacin showed sensitivity of 43.7% and gentamicin, 93.7%. But tetracycline showed tolerance of 31.3% , cefotaxine. 75%, nalidixic acid, 100%. L. monocytogenes of the 6 cases showed sensitivity of 100% in all 6 species, ciprofloxacin, etc.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.2
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• pp.192-197
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• 1999

Proximate composition, sterol and $\alpha$-tocopherol ($\alpha$-Toc) contents of 35 species of marine invertebrates (13 species of Bivalvia, 10 species of Gastropoda, 4 species of Cephalopoda, 4 species of Crustacea, 2 species of Ascidacea and 2 species of Holothuroidea), which caught off the coast of Tongyeong of the Southern sea (Nam-Hae), Korea, were determined. Protein content showed relatively high level in herbivorous (seaweed feeder) and carnivorous species such as Gastropoda ($17.2\pm4.30\%$), Crustacea ($16.8\pm4.06\%$) and Cephalopoda ($15.3\pm3.24\%$), but showed low in plankton feeder and mud swallow such as Bivalvia ($11.8\pm2.49\%$), Ascidacea ($8.20\pm1.00\%$) and Holothuroidea ($3.94\pm1.13\%$). Total lipid (TL) content was low in most marine invertebrates, ranged from $0.24\%$ (blue colored sea cucumbers) to $1.96\%$ (sea squirts). Ascidacea contained the largest amount of TL in all samples, while Holothuroidea contained the smallest amount of TL, and other classes contained about $1\%$ of TL. Carbohydrates was rich in plankton feeder and Herbivorous species (seaweed feeder) such as Ascidacea ($6.60\pm4.53\%$), Bivalvia ($3.15\pm1.82\%$) and Gastropoda ($3.02\pm1.61\%$), while poor in carnivorous species such as Crustacea ($0.52\pm0.57\%$) and Cephalopoda ($1.00\pm0.63\%$). Moisture content was highest in Holothuroidea ($92.0\pm1.94\%$) and the lowest in Gastropoda ($77.0\pm1.95\%$): There were negative correlations between moisture and protein content, or TL, between protein and TL content; y= -0.8716x+184.452 (r=-0.87, p<0.001), $y=128.52e^{-0.0601x}$ (r=-0.55, P<0.001), $y=6.3047e^{-0.0904x}$(r=-0.42, P<0.02), respectively. Sterol content was ranged from 56 mg/100 g edible portion of red colored sea cucumbers to 216 mg/100 g edible portion of cockle and was high in the following order; Cephalopoda (148 $\pm$30.0mg)>Bivalvia (121$\pm$35.0mg)>Gastropoda (118$\pm$20.0mg)>Crustacea (116$\pm$31.0mg)>Ascidacea (78.0$\pm$2.00 mg)>Holothuroidea(62.0$\pm$8.00 mg). Crustacea contained 1$\~$2 mg $\alpha$-Toc/100 g edible portion whereas other classes contained trace or less than 1 mg/100 g of edible portion.