• 한국초전도ㆍ저온공학회논문지
• /
• 제13권4호
• /
• pp.22-25
• /
• 2011

A single-coat $YBa_2Cu_3O_{7-{\delta}}$ (YBCO) film of high critical currents ($I_c$) could be successfully fabricated by optimizing the viscosity of the coating solution in the metal-organic deposition (MOD) process. From a Ba-deficient coating solution (Y: Ba: Cu = 1: 1.5: 3) having the viscosity of 212 $mPa{\cdot}sec$, 0.9 ${\mu}m$-thick single coat YBCO film with the $I_c$ value of 289 A/cm-width ($J_c$ = 3.2 MA/$cm^2$) at 77 K was achievable on the $SrTiO_3$ (STO) substrate, which was superior to that of our previous report for 0.8 ${\mu}m$-thick single coat YBCO film from a stoichiometric coating solution (Y: Ba: Cu = 1: 2: 3) on the $LaAlO_3$ (LAO) substrate. This result might be attributed to denser and more homogeneous microstrcuture in the case of the YBCO film from the Ba-deficient coating solution.

• 반도체디스플레이기술학회지
• /
• 제12권2호
• /
• pp.19-25
• /
• 2013

Extrusion is one of the most important operations in the polymer-processing industry. Balancing the distribution of flow through a die to achieve a uniform velocity distribution is the primary objective and one of the most difficult tasks of extrusion die design. If the manifold in a coat-hanger die is not properly designed, the exit velocity distribution may be not uniform; this can affect the thickness across the width of the die. Yet, no procedure is known to optimize the coat hanger die with respect to an even velocity profile at the exit. While optimizing the exit velocity distribution, the constraint optimization used in this work with allowable pressure drop in the die; according to this constraint we can control the pressure in the die. The computational approach incorporates three-dimensional finite element simulations software STAR-CCM+. These simulations are used with numerical optimization to design polymer coat hanger dies with pressure drop, uniform velocity and temperature variation across the die exit.

• 한국미생물·생명공학회지
• /
• 제42권2호
• /
• pp.152-161
• /
• 2014

The in vitro antimicrobial potential of the unexplored Moringa oleifera seed coat (SC) was evaluated against some Gram-positive and Gram-negative bacteria and yeast pathogens. Antimicrobial studies with various solvent extracts indicated ethyl acetate to be the best extractant, which was used for the rest of the antimicrobial studies as it tested neither toxic nor mutagenic. Gram-positive bacteria including a methicillin resistant Staphylococcus aureus (MRSA) strain were more susceptible with a minimum inhibitory concentration (MIC) range of 0.03-0.04 mg/ml. The antimicrobial pharmacodynamics of the extract exhibited both concentration-dependent and time-dependent killing. Most of the test organisms exhibited a short post antibiotic effect (PAE) except Enterococcus faecalis, Staphylococcus aureus, and Klebsiella pneumoniae 1, which exhibited longer PAEs. Amongst the major phytoconstituents established, flavonoids, diterpenes, triterpenes and cardiac glycosides exhibited inhibitory properties against most of the test organisms. The identified active phytochemicals of the M. oleifera seed coat exhibited antimicrobial potential against a wide range of medically important pathogens including the multidrug-resistant bugs. Hence, the M. oleifera seed coat, which is usually regarded as an agri-residue, could be a source of potential candidates for the development of drugs or drug leads of broad spectrum that includes multidrug-resistant bugs, which are one of the greatest concerns of the $21^{st}$ century.

• 한국추진공학회지
• /
• 제24권4호
• /
• pp.33-40
• /
• 2020

접착 보조제 종류에 따른 고체 추진제, 라이너 그리고 내열재의 접착 특성에 대한 연구를 수행하였다. 추진제/라이너/내열재 간의 가소제 또는 경화제의 이동을 막기 위하여 접착 보조제를 사용하였다. 접착 보조제의 종류는 Isocyanate계를 사용하였으며, 한 분자 내에 Isocyanate의 개수, 분자량에 대하여 접착 특성을 확인하였다. 시험 결과, -NCO기가 많을수록, 접착 보조제의 분자량이 클수록 접착력이 증가하는 것으로 확인하였다. 또한 접착 보조제를 도포한 후 가사시간에 따른 접착력을 비교한 결과, 가사시간이 짧을수록 접착력이 증가하는 것으로 확인되었다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제29권10호
• /
• pp.1392-1397
• /
• 2016

Guanine nucleotide-binding protein subunit alpha-s ($Gn{\alpha}s$) is a small subunit of the G protein-couple signaling pathway, which is involved in the formation of coat color. The expression level and distribution of $Gn{\alpha}s$ were detected by quantitative real-time-polymerase chain reaction (qPCR), western blot, and immunohistochemistry to investigate the underlying mechanisms of coat color in white and black skin tissues of mice. qPCR and western blot results suggested that $Gn{\alpha}s$ was expressed at significantly higher levels in black mice compared with that of white mice, and transcripts and protein possessed the same expression in both colors. Immunohistochemistry demonstrated $Gn{\alpha}s$ staining in the root sheath and dermal papilla in hair follicle of mice skins. The results indicated that the $Gn{\alpha}s$ gene was expressed in both white and black skin tissues, and the expression level of $Gn{\alpha}s$ in the two types of color was different. Therefore, $Gn{\alpha}s$ may be involved in the coat color formation in mice.

• 대한기계학회논문집B
• /
• 제24권11호
• /
• pp.1437-1446
• /
• 2000

A numerical analysis on the freeze coating process of a non-isothermal finite dimensional plate with a binary alloy is performed to investigate the growth and decay behavior of the solid and the mushy layer of the freeze coat and a complete procedure to calculate the process is obtained in this study. The continuously varying solid and mushy layers are immobilized by a coordinate transform and the resulting governing differential equations are solved by a finite difference technique. To account for the latent heat release and property change during solidification, proper phase change models are adopted. And the convection in the liquid melt is modeled as an appropriate heat transfer boundary condition at the liquid/mushy interface. The present results are compared with analytic solutions derived for the freeze coating of infinite dimensional plates and the discrepancy is found to be less than 0.5 percent in relative magnitude for all simulation cases. In addition the conservation of thermal energy is checked. The results show that the freeze coat grows proportional to the 1.2 square of axial position as predicted by analytic solutions ar first. But after the short period of initial growth, the growth rate of the freeze coat gradually decreases and finally the freeze coat starts to decay. The effects of various non-dimensional processing parameters on the behavior of freeze coat are also investigated.

• Journal of Plant Biotechnology
• /
• 제47권2호
• /
• pp.118-123
• /
• 2020

The black seed coat of soybeans contain anthocyanins which promote health. However, mature soybean seeds contain anti-nutritional factors like lipoxygenase, lectin and Kunitz Trypsin Inhibitor (KTI) proteins. Furthermore, these seeds can be used only after the genetic elimination of these proteins. Therefore, the objective of this study was to develop novel soybean genotypes with black seed coat and triple recessive alleles (lx1lx1lx2lx2lx3lx3, titilele) for lipoxygenase, lectin, and KTI proteins. From a cross of parent1 (lx1lx2lx3/lx1lx2lx3, ti/ti, Le/Le) and parent2 (lx1lx2lx3/lx1lx2lx3, Ti/Ti, le/le), 132 F₂ seeds were obtained. A 3:1 segregation ratio was observed during F₂ seed generation for the inheritance of lectin and KTI proteins. Between a cross of the Le and Ti genes, the observed independent inheritance ratio in the F₂ seed generation was 9: 3 : 3 : 1 (69 Le_Ti_: 32 leleTi_: 22 Le_titi: 9 leletiti) (χ²=2.87, P=0.5 - 0.1). From nine F₂ seeds with triple recessive alleles (lx1lx1lx2lx2lx3lx3, titilele genotype), one novel strain posessing black seed coat, and free of lipoxygenase, lectin and KTI proteins, was selected. The seed coat color of the new strain was black and the cotyledon color of the mature seed was green. The weight of 100 seeds belonging to the new strain was 35.4 g. This black soybean strain with lx1lx1lx2lx2lx3lx3, titilele genotype is a novel strain free of lipoxygenase, lectin, and KTI proteins.

• 식물병연구
• /
• 제18권3호
• /
• pp.231-235
• /
• 2012

본 연구는 citrus에 심각한 피해를 초래하는 바이러스인 ICRSV가 국내로 유입되는 것을 차단하여 그로 인한 피해를 방지하기 위해 이를 진단하는 시스템을 구축하고자 하였다. ICRSV가 감염된 시료를 구할 수 없어 외피단백질 유전자를 E. coli의 codon usage를 고려하여 optimization한 뒤 E. coli에서 수용성 단백질로 과발현된 재조합 ICRSV 외피단백질을 정제하였다. 정제한 재조합 단백질을 이용해 제작한 복클론 항체는 $1{\times}10^{-4}$으로 희석하였을 때 western blot과 ELISA를 통해서 각각 10 ng, 5 ng의 재조합 ICRSV 외피단백질을 검출할 수 있었다. 이로써 제작된 항체를 이용하여 소량의 바이러스 입자만으로도 ICRSV를 검출할 수 있을 것이다.

• Applied Microscopy
• /
• 제30권1호
• /
• pp.61-72
• /
• 2000

U. unicinctus 난자를 대상으로 인공수정 후 수정막 형성에 따른 물질적 기원과 첨체반응 등에 관련된 구조변화에 대하여 광학현미경과 전자현미경을 이용하여 관찰하였다. 관찰결과에 의하면 1차 수정막의 물질은 과잉의 정자에 대한 효과적 방어 기능을 보유한 난외막 표층 기원의 물질이었으며, 2차 수정막은 피질과립의 분비에 의하여 형성되었다. 3차 수정막은 광학현미경하에서 특이한 구조의 관찰은 없었으나 전자현미경에 의한 관찰시 많은 입자상 물질들로 구성되었으며, 수정 후 60분에는 이들 입자물질의 응집에 의해서 수정막은 경과되는 것으로 관찰되었다. 1차 수정막과 난외막 표층의 유사성 그리고 1차 수정막의 형성과 미세융모의 퇴화가 동시적인 사건이라는 것은 미세융모가 U. unicinctus에서 정자의 수용체로 작용할 수 있는 것으로 추정할 수 있었다. 첨체소관에 의한 난세포막 침투시 침체 단부에서 과립상의 물질이 표층(surface coat)에 확산되었다. 첨체소관은 첨체의 기부에서 시작되고, 첨체간격을 통하여 신장되었다.

• The Korean Journal of Physiology and Pharmacology
• /
• 제16권4호
• /
• pp.249-253
• /
• 2012

We analyzed the pharmacokinetics of C3G on data from twelve subjects, after 2-week multiple dosing of black bean (Phaseolus vulgaris, Cheongjakong-3-ho) seed coat extract, using the mixed effect analysis method (NONMEM, Ver. 6.2), as well as the conventional non-compartmental method. We also examined the safety and tolerability. The PK analysis used plasma concentrations of the C3G on day 1 and 14. There was no observed accumulation of C3G after 2-week multiple dosing of black bean seed coat extract. The typical point estimates of PK were CL (clearance)=3,420 l/h, V (volume)=7,280 L, Ka (absorption constant)=9.94 $h^{-1}$, ALAG (lag time)=0.217 h. The black bean seed coat extract was well tolerated and there were no serious adverse events. In this study, we confirmed that a significant amount of C3G was absorbed in human after given the black bean seed coat extract.