• Journal of Plant Biotechnology
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• v.44 no.2
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• pp.171-177
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• 2017

A callus-mediated regeneration protocol for sea-milkwort, an endangered coastal plant species in South Korea, is reported here. The explants of in vitro-plantlets generated from a node culture revealed distinguishable responses in callus induction depending on genotype, explant source, light condition, and 2,4-D concentration. Especially, continuous darkness exclusively facilitated callus induction from explants prior to other treatments. The calli initiated on the media with 2,4-D ranging from 0.1 mg/L to 3.0 mg/L in the dark vigorously proliferated when subcultured on the same media in continuous darkness. Given 1.0 mg/L zeatin in addition to darkness to the calli of the 'Pistachio' genotype, normal adventitious shoots were only regenerated from nodular structures that formed earlier from the calli at the frequency of 24.4 percent. Regenerated shoots easily grew into plantlets with roots and green color on a phytohormone-free MS medium under lighted condition, that were used for node culture as plant materials. Node culture effectively multiplied plantlets in accordance with protocol by Bae et al. (2016). Acclimatized plantlet clusters developed mature plant clusters under inland environment, followed by flowering the following April. Results were merged with node culture protocol suggested by Bae et al. (2016), which, as an in vitro propagation system for sea-milkwort, may contribute to natural habitat restoration.

• Journal of Life Science
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• v.18 no.10
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• pp.1355-1359
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• 2008

Endophytic fungi predominantly inhabit grasses, and produce a variety of beneficial metabolites for plant growth, as well as help their hosts against pathogens and herbivores. Current study was focused on plant growth promoting activity of endophytic fungi inhabited in the roots of sand dune grasses. We collected 49 fungal isolates from the roots of four most common sand dune grasses and screened them for their growth promoting capacity. Results showed that 37 fungal isolates (75.5%) promoted plant height and shoot length of waito-c rice, 11 fungal isolates (22.5%) suppressed it, while 1 fungus (2%) showed no effect on the growth attributes. The fungal strain Gibberella fujikuroi, along with distilled water and Czapek broth medium, were taken as control for this experiment. It was concluded that a major proportion of endophytic fungi inhabited in the sand dune plants produce metabolites, and thus help in growth and development of the host plant.

• Journal of Life Science
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• v.23 no.1
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• pp.95-101
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• 2013

In this study, plant samples of five species were collected from the Dokdo islands in South Korea. Plant samples such as Asparagus schoberioides, Corydalis platycarpa, Festuca rubra, Sedum oryzifolium, and Setaria viridis were collected from the Dongdo and Seodo. Endophytic fungal strains were isolated from the roots of five plants from the Dokdo islands. Thirty-three fungal strains were isolated from these native plants. All the endophytic fungi were analyzed by internal transcribed spacer (ITS) sequencing (ITS containing ITS1, 5.8s, and the ITS2 region). Waito-c rice seedlings were treated with fungal culture filtrates to test their plant growth-promoting activity. A bioassay of the D-So-1-1 fungal strain isolated from S. oryzifolium confirmed that it has the highest plant growth-promoting activity. All the endophytic fungi belong to four orders: Eurotiales (86%), Capnodiales (3%), Hypocreales (4%), and Incertae sedis (7%). The endophytic fungi were classified as Ascomycota, which contained Aspergillus (12%), Cladosporium (3%), Eurotium (3%), Fusarium (18%), Microsphaeropsis (6%), and Penicillium (58%) at the genus level.

• Korean Journal of Environment and Ecology
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• v.26 no.1
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• pp.11-38
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• 2012

Located in the middle part of the Korean peninsula, Pyeong-chang and Yeong-wol (Gangwon-do) areas are limestone regions that are important biogeographically showing the peculiar flora. In order to manage efficiently endangered and rare plant species, we have investigated flora of these above regions from March to October, 2010. The result of the investigation showed a total of vascular plants of 1,145 taxa; 134 families, 503 genera, and 1,010 species, 11 subspecies, 110 varieties, 10 forms and four hybrids. Among taxa investigated, endangered species designated by the law called 'Protection Law for Endangered wild fauna and flora' were seven taxa, and 50 taxa were considered to be endemic to Korea. Based on the floristic degrees categorized by the specific distribution of plant species, 24 taxa revealed the floristic degree (FD) V, 30 for FD IV, and 60 for FD III, 45 for FD II and 79 taxa for FD I. The 82 taxa were identified to be naturalized. Of those species investigated in this survey, 53 taxa were identified as specific ones biogeographically as compared to flora of other terrestrial regions; 35 of calcicolous plants, 15 with distribution in the northern part of the Korean peninsula, three with coastal habitats.

• Journal of Advanced Marine Engineering and Technology
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• v.37 no.2
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• pp.242-251
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• 2013

Recently, while global concern over offshore resources exploration and development is being increased rapidly, offshore plant industry is highlighted as an industry of high added value. Along with this global trend, domestic concern over offshore plant development is being increased as well. In the overseas case, a marine geotechnical survey guideline for confirming characteristics of seabed sediments is available at the time of installation of offshore plant but such guideline is not available in our country. In this study, survey techniques fit for domestic marine environment was applied according to overseas guideline at southern coastal area, Korea. Among the marine geophysical survey techniques being proposed abroad, magnetic survey and seabed photograph were excluded. However, highly reliable data analysis was enabled for marine geophysical survey, which includes in-situ coring investigation and laboratory soil test. In addition, continuous ocean current survey was included to find scour potential due to the current around the offshore plant. Although coring depth is not so deep, we predicted geological structure through the analysis of amplitude features of seismic data. Characteristics of seabed sediments cold be obtained regionally and directly through combined analysis of marine geophysical survey data and coring data.

• Journal of the Society of Disaster Information
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• v.13 no.3
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• pp.305-312
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• 2017

In our society, the advanced, advanced, and information industries have continued to grow and now live in the era of the fourth industrial revolution. As the industry develops, the load of the users has also increased so much that it is deepened by the energy shortage phenomenon and the construction of additional energy facilities is required. Therefore, energy plant construction work is being actively carried out in the coastal area. In particular, it is common to build a plant in the ground by filling the coast with soil in other regions, reflecting the fact that Korea is lacking in the country when constructing power plants, gas and petrochemical plants. Current domestic grounding designs are designed or constructed to suit only the use of grounding resistors based on the electrical equipment design technical standards. However, in the case of a plant facility constructed in the untested buried soil, when the lightning current and the abnormal current are inputted, the facility operator or the user due to the elevation of the ground potential is seriously exposed to the risk of electric shock disaster. In this paper, we analyze the ground resistivity of the landfilled soil and use a computer program (CDEGS) based on KS C IEC 61936-1, We analyze the contact voltage and stratification voltage and propose a grounding design optimized for plant installation.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.26 no.5
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• pp.314-326
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• 2014

On 31 March 2007, the abnormal wave occurred along the western coast of Korea. In order to investigate the generation mechanism of abnormal waves and to understand the amplification process of the abnormal waves, the observed data were analyzed and one-dimensional numerical model experiments were performed by using both the linear shallow water equation and the linear Boussinesq equation models. Various types of pressure jump for the abnormal waves previously proposed by other researchers were reviewed. As a result, it was not possible to reproduce the abnormal waves from the previously proposed pressure jumps. In this study, we proposed a new form of pressure jump, and numerical simulations were performed in order to check the validity of the proposed pressure jump. The numerical results showed that the calculated period of abnormal waves and the maximum water elevations agreed reasonably well with those of the observations.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.21 no.1
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• pp.45-53
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• 2009

A possibility of the drag coefficient estimation in numerical water basins was discussed where the numerical solution were calculated by the 3-dimensional hydro-dynamical model (FLOW-$3D^{(R)}$) with the RNG $k-{\varepsilon}$ turbulence model. On the known cases of the drag coefficients for a rectangle, the numerical drag coefficients got $1.34{\sim}1.52$ and the wind tunnel values were $1.3{\sim}1.5$. For a cylinder, the numerical values were calculated as $0.75{\sim}0.78$ in the range of 0.5

• Journal of Korean Society for Atmospheric Environment
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• v.23 no.4
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• pp.449-456
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• 2007

Dispersion coefficient preprocessing schemes have been examined to improve plume dispersion model performance in complex coastal areas. The performances of various schemes for constructing the sigma correction order were evaluated through estimations of statistical measures, such as bias, gross error, R, FB, NMSE, within FAC2, MG, VG, IOA, UAPC and MRE. This was undertaken for the results of dispersion modeling, which applied each scheme. Environmental factors such as sampling time, surface roughness, plume rising, plume height and terrain rolling were considered in this study. Gaussian plume dispersion model was used to calculate 1 hr $SO_2$ concentration 4 km downwind from a power plant in Boryeung coastal area. Here, measured data for January to December of 2002 were obtained so that modelling results could be compared. To compare the performances between various schemes, integrated scores of statistical measures were obtained by giving weights for each measure and then summing each score. This was done because each statistical measure has its own function and criteria; as a result, no measure can be taken as a sole index indicative of the performance level for each modeling scheme. The best preprocessing scheme was discerned using the step-wise method. The most significant factor influencing the magnitude of real dispersion coefficients appeared to be sampling time. A second significant factor appeared to be surface roughness, with the rolling terrain being the least significant for elevated sources in a gently rolling terrain. The best sequence of correcting the sigma from P-G scheme was found to be the combination of (1) sampling time, (2) surface roughness, (3) plume rising, (4) plume height, and (5) terrain rolling.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.29 no.1
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• pp.46-61
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• 2017

In the present work, the interaction analysis between tsunami bore and onshore bridge is approached by a numerical method, where the tsunami bore is generated by difference of upstream side and downstream side water levels. Numerical simulation in this paper was carried out by TWOPM-3D(three-dimensional one-field model for immiscible two-phase flows), which is based on Navier-Stokes solver. In order to verify the applicability of force acting on an onshore bridge, numerical results and experimental results were compared and analyzed. From this, we discussed the characteristics of horizontal force and vertical force(uplift force and downward force) changes including water level and velocity change due to the tsunami bore strength, water depth, onshore bridge form and number of girder. Furthermore, It was revealed that the entrained air in the fluid flow highly affected the vertical force.