• 한국수의병리학회지
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• 제5권2호
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• pp.43-48
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• 2001

Ducklings collected from three farms, having history of rapid onset and spread of nerve signs including kick spasmodically with legs and opisthotonos, were pathologically, barteriologically, virologically examined. Grossly, multiple petechial to ecchymotic hemorrhages were detected in the swollen liver. Histopathologically, diffuse coagulative necrosis of hepatocytes was characteristic in acute cases. Chronic cases revealed marked bile duct hyperplasia rather than hepatocyte necrosis. Some of these cases exhibited multiple granulomas consisting of macrophages, heterophil, fibrin and necrotic cell debri. Filtered homogenate of livers sampled from ducklings caused embryo death with marked hemorrhage and swollen of liver after inoculation into chorioallantoic membrane. Three strains of Salmonella app.,S montevideo, S hadar, and S give, which were biochemically and serologically identified, weir isolated from ducklings of three farms, respectively. From these results, these ducklings were concurrently infected with duck hepatitis virus and Salmonella spp.

• 대한수의학회지
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• 제7권2호
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• pp.46-50
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• 1967

The pathologic studies on the cottonseed meal poisoning in chicken were performed by feeding a diet containing 50 per cent cottonseed meal. The results obtained are summarized as follows: The specific effect of cottonseed meal on chicken was apparently limited to the parenchymatous tissue and blood vessels, where its major pathologic manifestations were degenerative changes in acute cases fed for a period of 7 days, while necrosis of portal and central veins of liver and consequent perivascular hemorrhage and coagulative necrosis of liver and intra-glomerular hemorrhage were characteristic lesions in chronic cases fed for a period 20, 40 and 60 days. In addition, specific cottonseed pigment cells were observed in small number in villus of small intestine in acute cases and in large number in villus, liver and spleen in chronic cases.

• 대한수의학회지
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• 제18권1호
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• pp.9-13
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• 1978

Nitrate poisoning was observed in Holstein calves raising for beef purpose in a dairy-farm of Gyeongbug province. The results observed were summarized as follows: 1. These calves had been fed mainly cornstalk, and a qualitative reaction by "dephenylamine blue test" for nitrate was positive in the serum of the affected calves and in the cornstalk provided. 2. In the clinical signs, the affected calves were suddenly recumbent without premonition and then dyspnea, followed by death. Death also occurred after inappetence, depression, dyspnea, cyanosis of mucous membrane, and terminal anoxic convulsions were observed. 3. In the macroscopical findings, the blood was dark brown (chocolate in color) and more or less poor coagulative. Petechiae of epicardium, severe cloudy swelling of liver, cloudy swelling and hemorrhage of kidney, congestion and hemorrhage of spleen, congestion and edema of lung, and congestion af abomasum and small intestine were observed in these cases. 4. Microscopical changes observed in these cases were peripheral congestion of lobules, centrilobular necrosis and sinusoidal dilatation in liver, hydropic degeneration of hepatic cells, hemorrhage and edema of kidney, necrosis of convoluted-tubular epithelium, and dilatation of Bowman's space and convoluted tubule. There were also congestion and hemorrhage of spleen, hemorrhage and edema of lung, cloudy swelling of myocardium, and congestion and hemorrhage of various organs.

• 한국임상수의학회지
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• 제16권1호
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• pp.218-221
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• 1999

A case of enetritis caused by Clostridium perfringens was observed in 4years old, male California sea lion(Zalophus californianus). Clinical signs were included in anorexia, depression, diarrhea and vomitting, and the animal died on 4 days after showing clinical signs. Grossly, bloody contents were presented in the intestine and the intestinal mucosa were congested and dark redcolored, and also there were serous fluid in the peritoneal cavity. Histopathologically coagulative necrosis destroyed two third of the villus. Clostridium perfringens were isolated from the intestinal contents. Based on the gross and histopathological findings, this case was diagnosed as necrotic enteritis caused by Clostridium perfringens infection.

• 한국동물위생학회지
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• 제29권4호
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• pp.497-501
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• 2006

Acute hemorrhagic enteritis was diagnosed in a seven-month-old male Shitzu dog dying of blood stained diarrhea and vomiting. Clinical findings were anorexia, dullness and sudden death after massive bloody diarrhea. At necropsy, main lesion was the hemorrhage in small intestine, mainly duodenum and jejunum. Microscopically, Gram positive long bacilli were massively detected on the mucose epithelial cells and necrotic debris of small intestine. Coagulative necrosis of epithelial cells and thrombosis of small intestine were also identified. However, there was no lesion of crypt epithelium. Mineral infiltration in both gastric mucosa and renal tubules was detected and proliferation of fibrous tissue was also shown in corticomedullary regions. In bacterial examination, C perfringens was isolated in anaerobic culture and it was confirmed to type A by multiplex PCR. Therefore, the dog was diagnosed as C perfringens type A associated enteritis with uremia.

• Applied Biological Chemistry
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• 제8권
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• pp.101-111
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• 1967

일당(日當) PMA $30{\gamma}$제I군(第I群), $3{\gamma}$제II군(第II群),그리고 $0.3{\gamma}$제III군(第III群)씩 90 일간(日間) 투여(投與)한 가토(家兎)에 있어서, 주요(主要) 장기(臟器)에 일어난 병리조직학적변화(病理組織學的變化)와 배설물(排泄物) 및 간장중(肝臟中)의 수은함유량(水銀含有量) 분석결과(分析結果)는 다음과 같다. 1. 신장(腎臟) : 제I군(第I群)에 있어서는 근위곡뇨세관상피세포(近位曲尿細管上皮細胞)에 심(甚)한 공포형성(空胞形成)과 혼탁종창(混濁腫脹)이 있었고, 근위직뇨세관상피세포(近位直尿細管上皮細胞)에 심(甚)한 혼탁종창(混濁腫脹) 및 응고괴사(凝固壞死)가 있었다. 또한 다수(多數)의 초자양원주(硝子樣圓柱)를 집합관내(集合管內)에서 볼수 있다. 제II군(第II群)에있어서나 위곡뇨세관상피세포(爲曲尿細管上皮細胞)에 중정도(中程度)의 공포형성(空胞形成) 및 혼탁종창((混濁腫脹)이있었고, 근위직뇨세관상피세포(近位直尿細管上皮細胞)에 중정도(中程度)의 혼탁혼탁종창(混濁混濁腫脹) 및 응고괴사(凝固壞死)가 있었다. 집합관내(集合管內)에는 소수(少數)의 초자양원주(硝子樣圓柱)가 있었다. 제II군(第II群)에 있어서는 근위곡세뇨관(近位曲細尿管) 및 근위직세뇨관상피세포(近位直尿細管上皮細胞)에 경도(輕度)의 혼탁종창(混濁腫脹)만을 보였다. 2. 간장(肝臟): 제I군(第I群)에 있어서, 간소엽(肝小葉)은 중심(中心)으로 혼탁종창(混濁腫脹), 지방변화(脂肪變化) 및 응고괴사(凝固壞死)를 보였고, 간세포색(肝細胞索)의 해리(解離)가 있었다. 제II군(第II群)에 있어서는 간소엽(肝小葉)은 간세포색(簡細胞索)의 정상구조(正常構造)를 유지(維持)하면서 활발(活潑)한 간세포증생(肝細胞增生)을 보였다. 제III군(第III群)의 간장조직(肝臟組織)은 병리학적변화(病理學的變化)를 보이지 않았다. 3. 비장(脾臟): 제I군(第I群)에있어서 혈철소(血鐵素)의 침착(沈着)이 현저(顯著)하였고 제II군(第II群)에서는 소량(小量)의 혈철소침착(血鐵素沈着)을 보였다. 제III군(第III群)에서는 별(別)다른 병리학적소견(病理學的所見)이 없었다. 4. 부신(副腎), 결장(結腸) 및 심장(心臟)은 각군(各群)에 있어서 아무런 병리적변화(病理的變化)를 보이지 안항T다. 5. 배설물중(排泄物中)의 수은함유량(水銀含有量)은 제I군(第I群)에 있어서는 총급여수은량(總給與水銀量) 0.45g에 대(對)해서 약(約) 76.5%가 배설(排泄)되었고 제II군(第II群)에 있어서는 85.44%, 제(第)III에 있어서는 79.89%가 각각(各各) 배설(排泄)되었다. 6. 간장중(肝臟中)의 수은함유량(水銀含有量)을 보면 제I군(第I群)에서 0.0348 g, 제II군(第II群)에서는 0.00378 g, 그리고 제III군(第III群)에서 0.00066 g이었다. 7. 일반적(一般的)으로 수은(水銀) 투여량(投與量)이 많으면 많을수록간장(肝臟)에서의 수은(水銀) 축적농도(蓄積濃度)가 높으나 총투여량(總投與量)에 대(對)한 축적량(蓄積量)은 이와 반대(反對)의 경향(傾向)을 보였다.

• Ocean and Polar Research
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• 제25권1호
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• pp.31-40
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• 2003

In August-September 2001, 15 samples of bottom sediments were collected in the inner, middle and open parts of Amursky Bay near Vladivostok, Russia, and barfin plaice Pleuronectes pinnifasciatus was sampled from the inner and the middle locations of the bay. In the sediments from all three sites elevated concentrations of several heavy metals, i.e. Zn ($102-115{\mu}/g$ dry weight), Ni $(70-73{\mu}g/g)$ and Cu $(27-35{\mu}g/g)$ were discovered. The contents of oil hydrocarbons were very close to or slightly higher than the maximal normal environmental background level, $100{\mu}g/g$ dry weight. The sediments contained negligible amounts of hexachlorocyclohexane, while DDT concentrations were quite high (1.7-16.3ng/g dry weight). Generally, there were no substantial differences in the pollution levels of the locations studied and our results resembled those reported for Amursky Bay in the 1990s. Surprisingly, in 2001 'fiesh' DDT comprised 70-85% of the total DDT content in sediment from all the locations studied. In fish liver total DDTs concentrations were 212.8 and 122.54 ng/g wet weight for the inner and the middle locations, respectively, and 'fresh' DDT comprised 35 and 64% of DDTs, respectively. These results provide evidence of recent input of DDT from an unknown source into the ecosystem of Amursky Bay. Histopathological changes revealed in the plaice liver (vacuolization of hepatocytes, coagulative necrosis of hepatocytes, inflammatory reaction, and necrosis of epithelial cells of bile ducts) are probably connected with an intensive metabolism of DDT in the fish organism. No histological and histomorphometric differences were found in the state of the interrenal tissue. Similar condition of the liver and the interrenal tissue in barfin plaice sampled from the inner and the middle locations of Amursky Bay may be explained by the absence of great differences in the pollution levels of these sites.

• 대한수의학회지
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• 제45권1호
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• pp.99-103
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• 2005

We report septicemic colibacillosis accompanied by white-spotted kidney in a 30-day old female Holstein-Friesian dairy calf. Grossly, there were numerous white spots sized average 0.5cm in diameter in both kidneys. When sectioned sagittally, there were radially oriented gray streaks extending outward and reaching the renal cortex. The renal papillae were ulcerated, white to gray in color and very friable. Histologically, there was extensive purulent inflammation characterized by severe neutrophilic cellular infiltrations in the tubular lumens and interstitia. In addition, massive coagulative necrosis were found in the apices of papillae. Numerous Gram-negative bacterial colonies were detected in both of the renal and lung tissues. Beta-hemolytic Escherichia coli (E. coli) was purely isolated from the renal parenchyma, peritoneal surface and pleural surface. Based on the above results, we suggest that the generalized septicemia with pyelonephritis may be oriented from the lower urinary tract infection with E. coli in this case.

• 한국동물위생학회지
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• 제46권4호
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• pp.349-355
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• 2023

A 7-year-old castrated male Pomeranian dog presented with severe goose honking cough and dyspnea. Thoracic radiographs revealed a narrowed tracheal diameter at the thoracic inlet, classified as tracheal collapse grade 4. Despite medical treatment, the dog's life-threatening airway obstruction did not improve. Subsequently, tracheal stent placement resulted in a significant improvement in respiratory condition, with no recurrence of symptoms observed during the 4-month period, except for coughing induced by excitement and anxiety. However, the patient presented with a one-week history of productive cough, exercise intolerance, and loss of appetite. Radiographs and computed tomography scans revealed torsion of the left cranial lung lobe. The patient underwent affected lung lobectomy, which involved the removal of the necrotized cranial portion and heavily congested caudal portion. Unfortunately, the patient did not recover and eventually passed away. Histopathological examination of the resected lung tissue confirmed coagulative necrosis and marked peribronchiolar edema, consistent with lung lobe torsion.

• 한국어병학회지
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• 제35권2호
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• pp.167-176
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• 2022

We injected infectious myonecrosis virus (IMNV) to pacific whiteleg shrimp, Litopenaeus vannamei, and observed closely with using light microscope and transmission electron microscope (TEM) for 4-8 days post infection (dpi). As clinical signs, abdominal bodies had mild opaque muscles at 5 dpi. And the mortality was shown at 6 dpi. At 8 dpi, most injected shrimps had severe opaque muscles and humped back that cause of movement disorder. As results of histopathological examinations, local parts of abdominal body muscle had muscle fiber hyalinization, muscle fiber atrophy, rounded muscle fibers, myofibrillar hypertrophy in size, a decrease in number of myofibrils and phagocytosis from the sarcolemmas by multiple hemocytes at 4 dpi. Especially, myofibrillar hypertrophy appeared at the whole or random part of single muscle fiber not in specific locations like the center or edge of muscle fiber. At 6-7 dpi, multiple muscle necrosis, muscle fiber segmentation, myofibril lysis ap- peared and a few hemocytes were infiltrated at lesions. At 8 dpi, extensive muscle necrosis, multiple myofibril lysis and muscle fiber atrophy were shown, and very few hemocytes were infiltrated. In early stage of infection, local viral myositis with zenker's degeneration were shown. These lesions appeared multiply after the early stage. In late stage of infection, extensive coagulative muscle necrosis appeared with few of inflammatory response such as hemocytes infiltration. The lack of hemocytes infiltration response at the late stage might be disadvantage for Litopenaeus vannamei to defense against IMNV and to recover, because hematocytes (granulocyte, semi-granulocyte) eliminate pathogen and damaged tissues from infection sites and help recover. As results of the TEM observation, IMNVs that had nonenveloped icosahedral capsid which was 30-40 nm diameter were in myofibril and beside tubules of sarcoplasmic reticulum and moved to the certain direction. The micro-tears and micro-trau- mas in myofibrils caused muscle fiber necrosis. And semi-granulocytes engulfed IMNVs to eliminate virus.