• 한국수산과학회지
• /
• 제29권5호
• /
• pp.620-628
• /
• 1996

여러 다른 S. aureus strains및 토끼항체를 사용하여 항체감작을 시킬 때 나타나는 S. aureus의 자체응집을 방지키 위한 분석과 함께 cogglutination의 최적 조건을 확립하였다. 적정화된 coagglutination기법을 실험실과 현장에서의 edwardsiellosis의 진단에 응용하였을 때 약 $10\;{\mu}g/m1$의 E. tarda까지 검출 할 수 있었다. 더구나 이 방법은 E. tarda의 FKC, EDTA또는 열탕추출 항원에 대해서 까지 좋은 진단결과를 보여주었다. 현장에서 edwardsiellosis에 감염된 어류로부터 직접 분리된 E. tarda 균주들은 토끼 항체생성을 위해 사용된 E. tarda 219와 응집항체법및 cogglutination법에서 모두 교차반응을 보여 주었다. 이러한 교차반응의 정도는 현장에서 나타나는 여러다른 E. tarda 균주에 감염 될 수 있는 어류의 질병진단에 사용하기에 충분한 정도로 나타났으며 감염어의 조직마쇄물을 1000배 이상 희석하여도 토끼 항 E. tarde 항체로 감작시킨 S. aureus와 coagglutination 될수 있는 양의 E. tarda를 함유하고 있었다. 자연감염 또는 인위감염된 넙치, 틸라피아의 조직마쇄물, 열탕추출항원에 대한 이 방법의 적용 결과는 본방법이 특별한 장비없이 현장에서 질병진단 기법으로 사용할 수 있다는 것을 보여 준다.

• 한국임상수의학회지
• /
• 제14권1호
• /
• pp.37-41
• /
• 1997

For the inspection of the occurrence situation of porcine pleuropneumonia and serotyping of Actinobacillus pleuropneumoniae strains isolated from lung lesions of pig in Korea, a series of experimentation have been carried out by the isolation and identification of A pleuropneumoniae, serotyping by coagglutination test, observation of lung lesion and clinical signs from 360 cases of porcine pneumonia in Clinical Pathology Laboratory, Bayer Veterinary Medical Research Institute. The results could be summarized as follows. The reaction of coagglutination between the reference antigens and the specific reagents of A pleuropneumoniae was strongly agglutinatied within 30 seconds without cross reaction. The 89 strains of A pleuropneumoniae were isolated from 360 cases of porcine pleuropneumonis and the biochemical properties of the isolates were same as the reference strains. The 89 isolated strains could be serotyped 39 strains as setotype 5, 34 strains as serotype 2, 8 strains as serotype 3, 2 strains as serotype 7 by coagglutination test, respectively. The clinical signs of pleuropneumonia were weakness, fever, anorexia, dyspnea and laboured breath in the later stages. The gross lesions of lung were haemorrhages, enlargement of interlobular septa, nodular formation and adhesion of the pleura.

• 대한미생물학회지
• /
• 제14권1호
• /
• pp.11-15
• /
• 1979

Identification of group A $\beta$-phemolytic streptococci is very important to provide an appropriate preventive measure of possible rheumatic fever and acute glomerulonephritis. For such purpose bacitracin susceptibility of streptococci because of its simplity has been most widely used despite of its occasional faulty results. Recently, a coagglutination technique was advocated using streptococcal group specific antibodies adsorbed to protein A-containing staphylococci. This study was conducted to evaluate the coagglutination technique using reagents prepared by ourselves. The specificity, reproducibility and stability were ascertained and the following results were obtained. 1. The identification by coagglutination technique using our own reagent gave the same results compared with the Lancefield precipitation technique. The result also agreed with the Phadebact grouping. 2. There were no variation in group A and B identification due to lot difference. However, there were a few discrepant results in group C and G identification which was conducted in different days with different lots of our reagent. 3. The stability of our reagents was less satisfactory compared to the commercial product. An effort to improve the stability was considered necessary. 4. For coagglutination, it was found convenient to use supernatant of Todd-Hewitt broth incubated for 24 hours. Both parafin-ringed slide glass and RPR card gave comparable results and the former could be used when the latter is not available.

• 대한미생물학회지
• /
• 제22권4호
• /
• pp.445-451
• /
• 1987

Coagglutination method is widely used for the diagnosis of Salmonella infection. This test, however, has a disadvantage of false positive reaction due to the coagglutination of staphylococci with non-specific immune complexes or anti-staphylococci antibody in serum. Salmonell O antigen was detected by enzyme immunoassay with protein A-bearing Staphylococcus aureus as in the solid phase. Horse radish peroxidase was labeled to IgG specific against Salmonella O antigen. This enzyme immunoassay was much more sensitive than conventional coagglutination method without false poitive agglutination. To improve the sensitivity for detection of Salmonella O antigen in samples, we tried to determine the optimal concentration of normal IgG that inhibits non-specific binding of horse radish peroxidase labeled IgG to staphylococci, and to establish the optimal condition of reaction between antigen-antibody complex and staphylococci. Non-specific binding of horse radish peroxidase labeled specific IgG to staphylococci was almost blocked when the enzyme labeled IgG was 500-fold diluted with phosphate buffered saline containing 2mg/ml of normal IgG. When staphylococci coated with antibody to Salmonella O antigen were mixed with antigen-antibody complex and then incubated for 1 hour at room temperature, the minimal detectable concentration of Salmonella O antigen was 1ng/ml. The sensitivity of enzyme immunoassay was 100-fold greater than a conventional coagglutination method. This enzyme immunoassay could be expected as an improved method for detection of other infectious agents.

• 한국산학기술학회논문지
• /
• 제11권7호
• /
• pp.2451-2458
• /
• 2010

고혈압 치료제인 카르베딜롤을 모델약물로 하여 새로운 서방성 방출 제어형 매트릭스 정제를 제조하기 위하여 소수성 서방성 부형제인 Compritol 888 ATO와 친수성 고분자인 hydroxypropyl methyl cellulose (HPMC) 또는 polyethylene oxide (PEO)를 이용하여 방출 제어형 매트릭스 정제를 제조하였다. 카르베딜롤 방출 제어형 매트릭스 정제의 제조 시 Compritol 888 ATO의 비율과 친수성 고분자의 종류 및 비율, hot melt coating coagglutination (HMCC) rocess의 적용 유무에 따른 카르베딜롤의 방출 양상을 위하여 용출시험기를 사용하여 pH 1.2의 인공위액과 pH 6.8의 인공장액에서 24시간 동안 $37^{\circ}C$, 50 rpm으로 용출시험을 실시하였다. 그 결과, HMCC process를 적용한 모든 제제가 약물의 방출 제어에 매우 효과적인 것을 확인하였다. 또한 소수성 서방성 부형제인 Compritol 888 ATO의 비율에 따라 약물의 방출 양상 및 시간이 기존 일반정제에 비하여 약 95%의 용출률을 나타내었으며 24시간까지 지연됨을 확인할 수 있었다.

• 대한수의학회지
• /
• 제37권3호
• /
• pp.577-582
• /
• 1997

To investigate the prevalence of Streptococcus suis in pig industry in Korea, we isolated S suis from pigs during two years. The isolates were identified by biochemical and coagglutination test. Serotypes and antimicrobial susceptibility of the isolates were determined. Fourteen strains(12.2%), 11 strains(27.5%), 58 strains(7.8%) and 4 strains(11.1%) of S suis were isolated from 115 nasal swabs, 40 tonsils of healthy pigs, 745 pneumonic lungs and 36 meningitis of diseased pigs, respectively. The isolates were highly susceptible to ampicillin, cephlothin and ofloxacin while they were resistant to oxytetracycline. Among the isolates, 63(75.9%) strains were to the S suis capsular type 1 to 10 and 11(13.3%) strains were untypable. Capsular type 2 was the most prevalent with 32(38.6%) strains of all isolates.

• 미생물학회지
• /
• 제24권1호
• /
• pp.73-78
• /
• 1986

N. gonorrhoeae 81균주를 320개의 가검물에서 분리동정한 후에 증식에 대한 VCN의 영향과 lsovitalex의 양에 따른 영향, 당분해능에 관한 실험과 ${\beta}-lactamase$생산균의 검출 및 plasmid분리에 대하여 연구하였다. 전체 320개의 가검물중에서 81균주의 N.gonorrhoeae가 분리동정되었고, chocolate agar에서는 81균주 중 72균주가,1% Isovitalex와 1 % VCN을 함유한 Thayer- Martin배지에서는 80균주가, 2 % Isovitalex와 1 % VCN이 든 Thayer-Martin 배지는 55균주가 N. gonorrhoeae의 특성을 나타냈다. Coagglutination시험에 양성인 81균주 중에서 67균주는 48시간 배양후에,10균주는 72시간 배양후에 glucose 배지에서 산을 생산하였으나, 나머지 4균주는 96시간 배양후에도 산을 생산하지 않았다. 인천지역 특수직업여성에서 분리된 81균주의 N.gonorrhoeae 중에서 41 균주가 ${\beta}-lactamase$를 생산하였고, ${\beta}-lactamase$ 생산 균주인 N. gonorrhoeae PL-118은 2.6. 4.5 및 24.5Mdal의 plasmid DNA를 포함하였다.

• 한국임상수의학회지
• /
• 제11권2호
• /
• pp.507-516
• /
• 1994

The purposes of this investigations were survey of the occurred bacterial diseases, development of new animal health drug, guidance to formers on the treatment and control methods of diseases. Some series of investigations have been carried out by microbiological, pathological and serological examinations. The results could be summarized as follows. 1. A total of 953 cases of outbreaked swine diseases have been diagnosed in Clinical pathology laboratories, Bayer Vet Res Institute during 8 years (from 1986 to 1993). The high incidence diseases were colibacillosis, pleuropneumonia, streptococcal infection and pasteurellosis in decreasing order. 2. Pleuropneumonia caused by Actinobacillus pleuropneumoniae was the most important respiratory diseases and pasteurellosis by Pasteurella multocide could be confirmed in several cases. 3. Actinobacillus pleuropneumoniae 50 strains were isolated and identified by biochemical and serological tests. In serotyping test, 22 isolated strains were serotype 5, 21 strains as serotype 2, each 2 strains as serotype 3 and 7 by the coagglutination test. 4. Colibacillosis and edema discase caused by Escherichia coli has been the most predominant outbreaked disease in this investigations. The 100 isolates of E coli strains were sensitive to amikacin, colistin, enrofloxacin, gentamycin and trimethoprim -sulfamethoxazole. 5. Swine erysipelas caused by Erysipelothrix rhusiopathiae was confirmed 25 cases as acute septicemic forms. Isolates of E rhusiopathiae were highly sensitive to ampicillin, cephalothin, enrofloxcin, penicillin and tetracycline. 6. The 49 cases of hemorrhagic and necrotic enteritis in piglets were observed and 13 strains of Clostridium perfringens could be isolated and confirmed by biological and serological test. Isolates of Clostridium perfringens type C were highly sensitive to ampicillin, cephalothin, enrofloxacin, penicillin and trimethoprim- sulfamethoxazole. 7. The 14 strains of Streptococcus suis type II could be isolated from meningitis of piplets. 8. Polyserositis caused by Haemophilus parasuis and salmonellosis were observed and confirmed. Also Corynebacterial infections and several parasitosis have been also observed in this investigations.