• 제목/요약/키워드: Clonal selection

검색결과 53건 처리시간 0.033초

A High Yield and Processing Potato Cultivar 'Taedong Valley'

  • Dhital, Shambhu P.;Lian, Yu J.;Hwang, Won N.;Lim, Hak T.
    • 한국육종학회지
    • /
    • 제42권3호
    • /
    • pp.207-211
    • /
    • 2010
  • 'Taedong Valley' is a high yielding and processing potato cultivar, which is a clonal selection resulting from a cross between 'W870' and 'A88431-1'. It is a medium maturating cultivar with medium plant height and light green foliage. 'Taedong Valley' has profuse flowering habit and light purple flowers. Tubers are smooth, round, and with yellow skin, light yellow flesh, medium eye depth. Tubers have medium dormancy and good keeping quality. 'Taedong Valley' has stable yield under wide range of climatic conditions. It is resistant to common scab and potato virus Y, but moderately susceptible to late blight. It is also resistant to most of the disorders, particularly dehiscence, hollow heart and internal brown spots. This cultivar has high level of tuber uniformity and capable of yielding 43.6 t/ha which is about 9.0% higher than the control potato cultivar 'Atlantic' under optimum agronomical practices.

Assessing the Performance of Pongamia pinnata (l.) Pierre under Ex-situ Condition in Karnataka

  • Divakara, Baragur Neelappa;Nikhitha, Chitradurga Umesh
    • Journal of Forest and Environmental Science
    • /
    • 제38권1호
    • /
    • pp.12-20
    • /
    • 2022
  • Pongamia (Pongamia pinnata L.) as a source of non-edible oil, is potential tree species for biodiesel production. For several reasons, both technical and economical, the potential of P. pinnata is far from being realized. The exploitation of genetic diversity for crop improvement has been the major driving force for the exploration and ex situ/in situ conservation of plant genetic resources. However, P. pinnata improvement for high oil and seed production is not achieved because of unsystematic way of tree improvement. Performance of P. pinnata planted by Karnataka Forest Department was assessed based on yield potential by collecting 157 clones out of 264 clones established by Karnataka Forest Department research wing under different research circles/ranges. It was evident that the all the seed and pod traits were significantly different. Further, selection of superior germplasm based on oil and pod/seed parameters was achieved by application of Mahalanobis statistics and Tocher's technique. On the basis of D2 values for all possible 253 pairs of populations the 157 genotypes were grouped into 28 clusters. The clustering pattern showed that geographical diversity is not necessarily related to genetic diversity. Cluster means indicated a wide range of variation for all the pod and seed traits. The best cluster having total oil content of more than 34.9% with 100 seed weight of above 125 g viz. Cluster I, II, III, IX, XV, XIX, XXI, XXIII, XXVI and XXVII were selected for clonal propagation.

RAPD법을 이용한 고구마 품종간 유연관계 평가 (Evaluation of Genetic Relationship among Sweetpotato Cultivars Using Randomly Amplified Polymorphic DNA (RAPD) Analysis)

  • 이긍표;박권우
    • 원예과학기술지
    • /
    • 제16권1호
    • /
    • pp.18-20
    • /
    • 1998
  • 본 실험은 RAPD (random amplified polymorphic DNA)를 이용하여 국내에서 육성된 13개 품종의 고구마 (Ipomoea batatas)를 대상으로 유연관계분류 및 품종구분 가능성을 탐색하였다. RAPD를 이용하여 고구마 품종을 비가중산술법(UPGMA)으로 3개의 그룹표로 분류할 수 있었는데 그룹 I은 '충승 100호'로, 그룹 II는 '은미', '생미', '수원147호'와 '율미', 그룹 III는 '홍미', '진미', '관동95', '선미', '원미', '신율미', '증미', '풍미'로 나뉘어졌다. RAPD를 이용한 분류 결과는 대체로 육성모부본의 유전자형과 일치함을 나타내고 있고, 상이한 점은 영양계의 변이에 의한 것으로 추측된다, 앞으로 이러한 marker system을 이용하여 육종시 조기에 원하는 형질을 갖는 계통을 선별할 수 있을 것이며 이에 따라 다양한 고구마 품종의 육종프로그램과 품종판별에 유용하게 이용될 수 있을 것으로 사료된다.

  • PDF

인공면역 시스템 기반 자율분산로봇 시스템의 협조 전략과 군행동 (Cooperative Strategies and Swarm Behavior in Distributed Autonomous Robotic Systems based on Artificial Immune System)

  • 심귀보
    • 한국지능시스템학회논문지
    • /
    • 제9권6호
    • /
    • pp.627-633
    • /
    • 1999
  • 본 논문에서는 면역 시스템에 기반한 자율분산로봇 시스템의 협조 제어 및 군행동 전략의 결정 방법을 제안한다. 면역 시스템은 생체의 자기보호 및 유지시스템이다. 면역 시스템의 유용한 성질은 동적으로 변하는 환경에서 최적의 군행동을 결정하는 문제에 적용 가능하다. 면역 시스템을 자율분산로봇 시스템에 적용하기 위하여 로봇은 B-세포로 환경조건은 항원으로 행동 전략은 항체로 제어파라미터는 T-세포로 각각 모델링 하였다, 환경(항원)변화가 감지되면 각 로봇은 적절한 행동전략(항체)을취한다. 이행동전략은 다른 로봇과의 통신에 의하여 자극 또는 억제을 받는다.(면역 네트워크) 최정적으로 많은 자극을 받은 전략이 군행동 전략으로 채택된다. 이 제어방법은 클론선택과 면역네트워크 가설에 기반을 둔것으로서 최적의 군행동 전략을 결정하는데 이용된다. 또한 제어 파라미터로서 T-세포 모델을 추가함으로서 동적인 환경에서 로봇의 적응능력이 향상되었다.

  • PDF

소나무류 육종에 있어 임의 증폭 다형 디엔에이(RAPD)지표를 이용한 우량 임목의 조기 선발 (Application of RAPD Markers to Early Selection of Elite Individuals of Pinus Species for a Clonal Forest Tree Breeding Program)

  • 이재선;정은주;문홍규;글렌 데일;로버트 티즈데일
    • Journal of Forest and Environmental Science
    • /
    • 제11권1호
    • /
    • pp.81-101
    • /
    • 1995
  • 지표-형질의 상관은 우량 개체 선발과 유전획득량의 증대를 위해 임목 육종에서 해결되어야 할 중요한 과제 중의 하나로 최근 분자유전학적 수준에서의 임의 증폭 다형 디엔에이 (RAPD) 기술의 발달로 이의 해결이 눈 앞에 다가왔다. 호주 퀸즈랜드산림청과 퀸즈랜드대 임목생물공학연구소가 공동 연구하고 있는 슬래쉬소나무, 카리비아소나무 및 그 교잡종에 있어 이 기술을 이용한 수피 두께에 대한 연구 및 육종 계획 전략을 소개한다. 1대 잡종에서 186개의 지표를 포함한 총 길이 1641cM의 16개 연관군의 유전적 지도가 작성되었고, 이 연관군 지도에 수피 두께를 지배하는 6개의 유전자좌가 추정되었다. 또한, 유전적 지표를 이용한 조기 선발을 위해 먼저 중요 형질을 지배하는 유전자들에 대한 종 특성 유전적 지표를 결정하고, 다음 여러가지 대립유전자형에 대한 지표-대립유전자 상관을 구명하는 2단계 전략이 제시되었다. 소나무류는 발아시 양료로 쓰이는 자성배우체는 모수에서 유래하나, 접합자인 배는 양친수로부터 유래하므로 이러한 이질적 유전 조성을 갖인 종자의 발달을 이용한 RAPD 지표와 형질의 상관 연구는 배 단계에서도 우량 개체의 선발을 가능하게 하여 소나무류 육종의 장래를 밝게 하고 있다.

  • PDF

Zoning Permanent Basic Farmland Based on Artificial Immune System coupling with spatial constraints

  • Hua, Wang;Mengyu, Wang;Yuxin, Zhu;Jiqiang, Niu;Xueye, Chen;Yang, Zhang
    • KSII Transactions on Internet and Information Systems (TIIS)
    • /
    • 제15권5호
    • /
    • pp.1666-1689
    • /
    • 2021
  • The red line of Permanent Basic Farmland is the most important part in the "three-line" demarcation of China's national territorial development plan. The scientific and reasonable delineation of the red line is a major strategic measure being taken by China to improve its ability to safeguard the practical interests of farmers and guarantee national food security. The delineation of Permanent Basic Farmland zoning (DPBFZ) is essentially a multi-objective optimization problem. However, the traditional method of demarcation does not take into account the synergistic development goals of conservation of cultivated land utilization, ecological conservation, or urban expansion. Therefore, this research introduces the idea of artificial immune optimization and proposes a multi-objective model of DPBFZ red line delineation based on a clone selection algorithm. This research proposes an objective functional system consisting of these three sub-objectives: optimal quality of cropland, spatially concentrated distribution, and stability of cropland. It also takes into consideration constraints such as the red line of ecological protection, topography, and space for major development projects. The mathematical formal expressions for the objectives and constraints are given in the paper, and a multi-objective optimal decision model with multiple constraints for the DPBFZ problem is constructed based on the clone selection algorithm. An antibody coding scheme was designed according to the spatial pattern of DPBFZ zoning. In addition, the antibody-antigen affinity function, the clone mechanism, and mutation strategy were constructed and improved to solve the DPBFZ problem with a spatial optimization feature. Finally, Tongxu County in Henan province was selected as the study area, and a controlled experiment was set up according to different target preferences. The results show that the model proposed in this paper is operational in the work of delineating DPBFZ. It not only avoids the adverse effects of subjective factors in the delineation process but also provides multiple scenarios DPBFZ layouts for decision makers by adjusting the weighting of the objective function.

국내 아까시나무의 용재 특성 향상을 위한 우량개체 선발 (Plus Tree Selection of Black Locust (Robinia pseudoacacia L.) for Tree Improvement of Timber Characteristics)

  • 김태림;임혜민;이경미;오창영;이일환;황환수
    • 한국자원식물학회지
    • /
    • 제36권1호
    • /
    • pp.91-99
    • /
    • 2023
  • 기후변화 대응 바이오매스 생산이 우수한 활엽수 경제 조림수종 개발에 대한 수요가 증가하고 있다. 아까시나무는 탄소흡수능이 우수한 속성수로서 국내 적응성이 뛰어나고 경제적, 환경적 기능이 우수하다. 본 연구는 비교선발법을 이용하여 용재수로서 가치가 높은 아까시나무 우량개체 64본을 선발하였다. 경기, 강원, 충청, 경상 등 4개 지역으로 나누어서 생장특성을 비교한 결과 선발목들은 비교목들에 비해 수고, 흉고직경, 지하고, 통직성에서 유의적으로 우수한 형질을 보였다. 특히 충청지역의 선발목들이 가장 우수한 생장특성을 나타냈으며, 임분별로는 고성, 천안, 안동 집단이 우량집단으로 나타났다. 생장에 영향을 주는 요인 중에서 토양 성분은 상관관계가 없었으며, 대단위 산림 조림 지역 혹은 호수와 저수지가 인접한 지역의 선발목이 수고 생장이 우수한 것으로 나타났다.

Acacia mangium Willd. - A Fast Growing Tree for Tropical Plantation

  • Hegde, Maheshwar;Palanisamy, K.;Yi, Jae Seon
    • Journal of Forest and Environmental Science
    • /
    • 제29권1호
    • /
    • pp.1-14
    • /
    • 2013
  • Acacia mangium is an evergreen fast-growing tropical tree, which can grow up to 30 m tall and 50 cm thick, under favorable conditions. It is a low-elevation species associated with rain forest margins and disturbed, well-drained acid soils. It is native to Papua, Western Irian Jaya and the Maluku islands in Indonesia, Papua New Guinea and north-eastern Queensland in Australia. Due to its rapid growth and tolerance of very poor soils, A. mangium was introduced into some Asian, African and western hemisphere countries where it is used as a plantation tree. A. mangium has good quality wood traits, such as a comparatively low proportion of parenchymatous cells and vessels, white and hard wood, and high calorific value. Therefore, it is useful for a variety of purposes, such as furniture, cabinets, turnery, floors, particleboard, plywood, veneer, fence posts, firewood, and charcoal. It is also being used in pulp and paper making because it has good pulp traits, with high yields of pulp, quality of kraft, and produces paper with good optical, physical and surface properties. Because there are significant provenance differences in growth rate, stem straightness, heartwood formation and frequency of multiple leaders, the productivity and quality also varies depending upon environmental conditions, so genetic improvement programmes have been undertaken in countries like Australia, India, Indonesia, Malaysia, the Philippines, Taiwan and Thailand. The programme includes provenance identifications and testing, plus tree selection and clonal multiplication, establishment of seed orchards and hybridization. The phenology, reproductive biology, fruit characteristics, silvicultural practices for cultivation, pest and diseases problems, production of improved planting stock, harvesting, wood properties and utilization have been discussed in this paper.

Rapid Establishment of CHO Cell Lines Producing the Anti-Hepatocyte Growth Factor Antibody SFN68

  • Song, Seong-Won;Lee, Song-Jae;Kim, Chang-Young;Han, Byungryeul;Oh, Jong-Won
    • Journal of Microbiology and Biotechnology
    • /
    • 제23권8호
    • /
    • pp.1176-1184
    • /
    • 2013
  • Anti-hepatocyte growth factor (anti-HGF) monoclonal antibodies (mAbs) are potential therapeutics against various cancers. Screening for high-producer clones is a time-consuming and complex process and is a major hurdle in the development of therapeutic mAbs. Here, we describe an efficient approach that allows the selection of high-producer Chinese hamster ovary (CHO) cell lines producing the novel anti-HGF mAb SFN68, which was generated previously by immunizing HGF bound to its receptor c-Met. We selected an SFN68-producing parental cell line via transfection of the dihydrofolate reductase-deficient CHO cell line DG44, which was preadapted to serum-free suspension culture, with an SFN68-expression vector. Subsequent gene amplification via multiple passages of the parental cell line in a methotrexate-containing medium over 4 weeks, followed by clonal isolation, enabled us to isolate two cell lines, 2F7 and 2H4, with 3-fold higher specific productivity. We also screened 72 different media formulated with diverse feed and basal media to develop a suboptimized medium. In the established suboptimized medium, the highest anti-HGF mAb yields of the 2F7 and 2H4 clones were 842 and 861 mg/l, respectively, which were about 10.5-fold higher than that of the parental cell line in a non-optimized basal medium. The selected CHO cell lines secreting high titers of SFN68 would be useful for the production of sufficient amounts of antibodies for efficacy evaluation in preclinical and early clinical studies.

Relationship between Cell Size and Specific Thrombopoietin Productivity in Chinese Hamster Ovary Cells during Dihydrofolate Reductase-mediated Gene Amplification

  • Kim, Tae-Kyung;Chung, Joo-Young;Sung, Yun-Hee;Lee, Gyun-Min
    • Biotechnology and Bioprocess Engineering:BBE
    • /
    • 제6권5호
    • /
    • pp.332-336
    • /
    • 2001
  • When parental Chinese hamster ovary (CHO) cell clones that are capable of producing thrombopoietin (TPO) were subjected to high methotrexate (MTX) concentrations, clonal variations in cell growth were apparent. In the clones that had no significant enhancement in specific TPO productivity (q$\_$Tpo/)when a higher level of MTX was administered, their growth was not depressed significantly nor their cell size changed significantly. On the other hand, those clones that showed a significant-enhancement in q$\_$Tpo/ at higher a MTX dosage, cell growth was depressed initially but recovered during successive sub-cultures. Furthermore, their cell size increased, which suggested that changes in cell size may be indicative of an enhanced q$\_$Tpo/. When the enhancement of the q$\_$Tpo/ of 9 clones after a high MTX dosage was plotted against the extent of the increase of their size, there was a linear correlation (γ$^2$=0.80, p<0.001, ANOVA), which suggested that an enhancement of q$\_$Tpo/ after high MTX administration can be measured by the increase in their cell size. Taken together, our data demonstrate that the selection of amplified CHO cell clones with enhanced q$\_$Tpo/ can be done upon their increased size and growth pattern. This facilitates the development of highly productive recombinant CHO cell lines.

  • PDF