• Korean Journal of Clinical Pharmacy
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• v.15 no.1
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• pp.61-67
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• 2005

This study was designed to establish a strategy for the bioequivalence study of doxifluridine, an anticancer drug, in dogs instead of cancer patients. Although the results from animals may not occur in the same manner from human, those would be worth enough in terns of the bioequivalence. As for critically ill population such as cancer patients, bioequivalence studies in animals bring many advantages. Six healthy Beagle dogs were selected on the basis of hematology and blood chemistry test. After an over night fast, 200 mg of doxifluridine was orally administered, and blood was serially taken up to 12 hours. Plasma concentration of doxifluridine was measured using a newly validated bioanalytical method by a HPLC coupled tandem mass spectrometry. Time course of plasma doxifluridine concentration was analyzed with non-compartmental and compartmental approaches. Consequently, we represented hematology and blood chemistry database for the selection of healthy Beagle dogs, and suggested a sensitive and validated analytical method of doxifluridine, as well as a study design for the bioequivalence of doxifluridine in dogs.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.18
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• pp.8197-8201
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• 2016

Background: To investigate the expression of the fragile histidine triad (FHIT) gene in acute lymphoblastic leukemia and its clinical significance. Materials and Methods: The level of expressed FHIT mRNA in peripheral blood from 50 patients with acute lymphoblastic leukemia (ALL) and in 50 peripheral blood samples from healthy volunteers was measured via RT-PCR. Correlation analyses between FHIT gene expression and clinical characteristics (gender, age, white blood count, immunophenotype of acute lymphoblastic leukemia and percentage of blast cells) of the patients were performed. Results: The FHIT gene was expressed at $2.49{\pm}7.37$ of ALL patients against $14.4{\pm}17.9$ in the healthy volunteers. The difference in the expression levels between ALL patients and healthy volunteers was statistically significant. The rate of gene expression did not significantly vary with immunophenotype subtypes. Gene expression was also found to be correlated with increase of total leukocyte and decrease in platelets, but not with age, gender, immunophenotyping or percentage of blast cells. Conclusions: FHIT gene expression is low in acute lymphoblastic leukemia and could be a useful marker to monitor minimal residual disease. This gene is also a candidate target for the immunotherapy of acute lymphoblastic leukemia.

• Journal of Ginseng Research
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• v.44 no.2
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• pp.179-193
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• 2020

Ginseng products on the market show high variability in their composition and overall quality. This becomes a challenge for both consumers and health-care professionals who are in search of high-quality, reliable ginseng products that have a proven safety and efficacy profile. The botanical extract standardization is of crucial importance in this context as it determines the reproducibility of the quality of the product that is essential for the evaluation of effectiveness and safety. This review focuses on the well-characterized and standardized ginseng extract, G115, which represents an excellent example of an herbal drug preparation with constant safety and efficacy within the herbal medicinal products. Over the many decades, extensive preclinical and clinical research has been conducted to evaluate the efficacy and safety of G115. In vitro and in vivo studies of G115 have shown pharmacological effects on physical performance, cognitive function, metabolism, and the immune system. Furthermore, a significant number of G115 clinical studies, most of them double-blind placebo-controlled, have reinforced the findings of preclinical evidence and proved the efficacy of this extract on blood glucose and lipid regulation, chronic obstructive pulmonary disease, energy, physical performance, and immune and cognitive functions. Clinical trials and 50 years of presence on the market are proof of a good safety profile of G115.

• Journal of the Korean Chemical Society
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• v.42 no.6
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• pp.708-712
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• 1998

• Korean Journal of Veterinary Research
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• v.35 no.3
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• pp.625-630
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• 1995

During the period from june 1994 to february 1995 influence of free stall with concrete floor on profile of blood chemistry and clinico-morphopathogenesis of foot disease on 266 cows were studied. The results obtained as follow. 1. No differences between value of blood chemistry in normal and lame cows with foot disease were observed. 2. 20.7% of the cows were clinically lame. 3. Prevalence of clinical digital disorders were investigated: hyperplasia interdigitalis(45.8%), pododermatitis circumscripta(22.4%), dermatitis interdigitalis(9.4%), erosio ungulae(5.9%), phlegmona interdigitalis(3.5%), pododermatitis septica traumatica(3.5%), dermatitis digitalis(2.4%), white line disease(2.4%), pododermatitis aseptica diffusa(2.4%), dermatitis verrucosa(1.2%), fissura ungulae(1.2%) 4. Most claw lesions were located on lateral hindclaws and interdigital space of hindclaws.

• Bulletin of the Korean Chemical Society
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• v.15 no.10
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• pp.836-841
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• 1994

The polymer matrix effect on the selectivity, response rate and reproducibility for coextraction type anion-selective optode membranes were investigated with DOA-plasticized PVC, PVC/hydroxylated PVC, PU/hydroxylated PVC and DOS-plasticized CTA matrices. Optode membranes were prepared with TDMACl and ETH2412 dissolved in one of the four solvent polymeric matrices. The PU/hydroxylated PVC and PVC-based membranes have almost the same selectivity coefficients, while the CTA-based membrane is more selective toward lipophilic anions. The membrane with PU/hydroxylated PVC adhered strongly to a glass surface, and showed highly reproducible and relatively rapid response. Very poor adhesion of PVC/hydroxylated PVC and CTA-based membranes limited the usability of those membranes as sensor components. Based on these results, and considering the biocompatibility for clinical samples, the optode made with PU/hydroxylated PVC was applied to determine the thiocyanate ion in human saliva. The results obtained with this simple device were comparable to those with rather complicated ISE methods.

• Journal of Veterinary Clinics
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• v.16 no.1
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• pp.235-238
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• 1999

Two Jindo dogs which show clinical signs of anorexia, weakness and exercise intolerance, were referred, and diagnosed as dirofilariasis by hematology, blood chemistry and ultrasonography. In hematology and blood chemistry, the presence of microfilariae in the blood, anemia, leukocytosis, hemoglobinuria and jaundice were revealed. In ultrasonography, heartworms were found in the right ventricle artery and pulmonary artery as structures of hyperechoic dots or streaks. In autopsy, forty odd and fifty odd of heartworms at dog 1 and dog 2, were ascertained respectively in the right ventricle and pulmonary artery.

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.69-71
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• 2003

As recent as 10 years ago, a method of developing new medicine was developed by a new compounding method moving away from an existing one. Combinatorial chemistry made it easier to combine various kinds of compounds in a very short time and with little effort from existing methods. Through combinatorial chemistry, a number of compounds were synthesized using HTS(High Throughput Screening), with many reports reaching a clinical stage in search of new candidate material. (omitted)

• Journal of the Korean Chemical Society
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• v.52 no.6
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• pp.668-675
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• 2008

• BMB Reports
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• v.46 no.4
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• pp.188-194
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• 2013

Two-photon microscopy (TPM), which uses two photons of lower energy as the excitation source, is a vital tool in biology and clinical science, due to its capacity to image deep inside intact tissues for a long period of time. To make TPM a more versatile tool in biomedical research, we have developed a variety of two-photon probes for specific applications. In this mini review, we will briefly discuss two-photon probes for lipid rafts, lysosomes, mitochondria, and pH, and their biomedical applications.