• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2021년도 춘계학술대회
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• pp.16-16
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• 2021

To distinguish manchurian clematis, Clematis terniflora var. mandshurica and three-leaf clematis, C. apiifolia, we collected 9 nuclear ITS sequences and two sequences of trnQ-trnH intergenic spacer and trnH region in GenBank database. Those sequences were aligned to find differences between those of C. terniflora var. mandshurica and C. apiifolia. Two primer pairs were newly designed base on the differences between two species and conducted multiplex PCR. The size of amplified fragments using generated primers were 380 base pairs (only three-leaf clematis) and 189 base pairs (both species). This genetic marker based on PCR is useful to discrimination of C. terniflora var. mandshurica and C. apiifolia

• BMB Reports
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• 제51권8호
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• pp.400-405
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• 2018

Chronic stress induces neuronal cell death, which can cause nervous system disorders including Parkinson's disease and Alzheimer's disease. In this study, we evaluated the neuroprotective effects of Clematis terniflora extract (CTE) against corticosterone-induced apoptosis in rat pheochromocytoma (PC12) cells, and also investigated the underlying molecular mechanisms. At concentrations of 300 and $500{\mu}g/ml$, CTE significantly decreased apoptotic cell death and mitochondrial damage induced by $200{\mu}M$ corticosterone. CTE decreased the expression levels of endoplasmic reticulum (ER) stress proteins GRP78, GADD153, and mitochondrial damage-related protein BAD, suggesting that it downregulates ER stress evoked by corticosterone. Furthermore, our results suggested that these protective effects were mediated by the upregulation of p-AKT and p-ERK1/2, which are involved in cell survival signaling. Collectively, our results indicate that CTE can lessen neural damage caused by chronic stress.

• Horticulture, Environment, and Biotechnology : HEB
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• 제59권6호
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• pp.889-897
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• 2018

Currently, there is a lack of genetic markers capable of effectively detecting polymorphisms in Clematis. Therefore, we developed new markers to investigate inter- and intraspecific diversity in Clematis. Based on the complete chloroplast genome of Clematis terniflora, simple sequence repeats were explored and primer pairs were designed for all ten adequate repeat regions (cpSSRs), which were tested in 43 individuals of 11 Clematis species. In addition, the nuclear ITS region was sequenced in 11 Clematis species. Seven cpSSR loci were found to be polymorphic in the genus and serve as markers that can distinguish different species and be used in different genetic analyses, including cultivar identification to assist the breeding of new ornamental cultivars.

• 한국환경복원기술학회지
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• 제2권1호
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• pp.72-82
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• 1999

The objective of this study was to covering of vine the traffic noise barrier and analyse of their growth characteristics. For the purpose of the experiments, chosen were 28 plants. In January 1996 to December 1998, the nursery seedbeds on the Chonju Arboretum in Korea Highway Corporation were seeding and cutting with those species, and a few species were carried out Honam Highway field experiments. The results are summarized as follows ; The germination ratio of seedbed experiments were Wisteria floribunda(88%), Lonicera japonica(86%), Parthenocissus tricuspidata, Paederia scandens(85%), Celastrus orbiculatus(76%), Clematis terniflora var. denticulata(52%), Clematis mandshurica(44%) respectively. The rooting ratio of cutting experiments were Trachelospermum asiaticum var. intermedium(88%), Parthenocissus quinquefolia(87%), Trachelospermum asiaticum var. asiatica(85%), Kadsura japonica(82%), Hedera rhombea(81%), Euonymus fortunei var. radicans(83%), Trachelospermum jasminoides var. pubescens(80%), Lonicera japonica cv. Aureo-reticulata(80%), Trachelospermum asiaticum var. majus(78%), Euonymus radicans cv. Aureo-marginata(66%), in descending order. As a conclusion, this study shows that the traffic noise barrier was effected by Paederia scandens, Lonicera japonica, Parthenocissus quinquefolia, Wisteria floribunda, Parthenocissus tricuspidata in the field experiments and the others were necessary to maintainable management.

• 농약과학회지
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• 제8권3호
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• pp.220-230
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• 2004

본 연구의 목적은 내의 자생식물 중 살포활성이 있는 식물 종을 선발하는데 있다. 국내의 자생식물 55과 98종 시료로터 MeOH 조추출물을 얻은 다음 24-well plate에서 유채(Brassica napus L.)에 대한 살초검성을 수행하였다. 본 연구에 사용된 98종 중 놀은 살포활성($GR_{50}$ 값 < 1,000 ${\mu}g\;g^{-1}$)을 나타낸 식물은 가중나무(Ailanthus altissima (MILL.) SWINGLE), 고비(Osmunda japonica THUNB.), 더위지기(Artemisia iwayomogi KITAMURA), 두릅(Aralia elata SEEM.), 떡쑥(Gnaphalium affine D. DON), 미국산사나무(Crataegus scabrida SARG.), 사군자(Quisqualis indica L.), 센달나무(Machilus japonica S. et Z.), 소귀나무(Myrica rubra S. et Z.), 실육카(Yucca smalliana FERN.), 양버즘나무(Platanus occidentalis L.), 영춘화(Jasminum nudiflorum LINDL.), 유채(Brassica campestris subsp. napus var. nippo-oleifera MAKINO), 음나무(Kalopanax pictus (THUNB.) NAKAI), 자리공(Phytolacca esculenta V. Houtte), 장딸기(Rubus hirsutus THUNB.), 젓나무(Abies holophylla MAXIM.), 족도리풀(Asarum sieboldii MIQ.), 참으아리(Clematis terniflora DC.), 캐모마일(Anthemis nobilis L.) 등 20종으로 나타났다. 자생식물 98종 중 50종 식물은 중정도의 살초활성을 나타내었으나(1,000 ${\mu}g\;g^{-1}$ < $GR_{50}$ 값 < 2,000 ${\mu}g\;g^{-1}$), 28종 식물은 살초활성을 전혀 나타내지 않았다.

• 대한본초학회지
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• 제38권5호
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• pp.39-47
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• 2023

Objectives : The objective of present study was to investigate the vasorelaxant effects of 20 Korean native plants on isolated rat thoracic aorta precontracted with phenylephrine (PE). Methods : Dried 20 plant materials were extracted 3 times with water, ethanol, or methanol for 3h in the reflux apparatus at 70 ± 5℃. Male SD rats were anesthetized by ether inhalation, and their aorta rings were isolated and placed in 10 ㎖ Krebs Henseleit (KH) buffer. While using an isolated organ-chamber technique, the aorta rings were maintained by bubbling with a gas mixture of 95% O₂-5% CO₂ at 37℃. Changes in isometric tension of aorta rings were recorded via isometric transducers connected to a Powerlab Data Acquisition System. Results : Among the 20 native plants, Chrysanthemum indicum L. flower, Nelumbo nucifera Gaertn. rhizome, Schisandra chinensis (Turcz.) Baill. fruit, Anthriscus sylvestris (L.) Hoffm. root, Corydalis turtschaninovii Besser tuber, Corydalis decumbens (Thunb.) Pers. tuber, and Dolichos lablab L. seed showed significant vasorelaxant effect on the contraction of aorta rings induced by PE. In contrast, Mertensia maritima subsp. asiatica Takeda whole plant, Ajuga decumbens Thunb. whole plant, Trigonotis peduncularis (Trevis.) Benth. ex Baker & S.Moore whole plant, Dioscorea quinquelobate Thunb. rhizome, Allium microdictyon Prokh aerial part, Momordica charantia L. fruit, Carthamus tinctorius L. flower, and Clematis terniflora DC. root constricted more the aorta rings precontracted by PE Conclusion : These results suggest that the possibility as useful herbal resources for the development of functional foods or medicines for hypertension treatment.

• 한국환경과학회지
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• 제12권11호
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• pp.1181-1187
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• 2003

Hibiscus hamabo, called "Hwang-geun", growing about 3m in height is a deciduous shrub or subtree of Malvaceae. Because the number of these species is very limited in the world, the Ministry of Environment has designated H. hamabo as a preserved plant. The Korea Forest Service also protects it strictly by law since H. hamabo is an out-of-the-way plant and possibly may be exterminated soon in Korea. Investigation for distribution and ecological characteristics of the habitat for H. hamabo was carried out on Soan Island. Two wild H. hamabo were found at the forest edge (equation omitted) along the sea coast located in the southern part of Soan Island and this was the first report in the Korean academic world. These two wild H. hamabos were growing in a naturally inhibited area. The diameters at the base were 12cm and 15cm. The Tree heights were 150cm and 210cm and the number of branches of each wild H. hamabo was 4 and 7. However, the present condition of these plants was not good. Environmental conditions of the naturally inhibited area of H. hamabo were very mild because it is located at the edge of the forest and is always sunny during the daytime since the slope of the inhibited area is facing South. The ground drained very well since the soil was made of gravels and sand. Because the percent of vegetation of the subtree layer where H. hamabo was growing was 40%, the cover degree and sociability of flex crenata trees and Eurya japonica were found to be high. In the naturally inhibited area of H. hamabo, a dominant value of Rubus parvifolius in the lower part of the herb layer was very high and many plants in Compositae, such as Artemisia princeps var. orientalis and Erigeron annuus, were also present. A dominant value of liana, such as Vitis thunbergii var. sinuata, Rosa multiflora, Clematis terniflora and Hedera rhombea, and Gramineae plants that rhizomes were well developed and aggressively propagated, such as Miscanthus sinensis var. purpurascens, Phragmites communis, Spodiopogon cotulifer and Oplismenus undulatifolius which were surveyed as high, too. These results imply that H. hamabo might be exterminated soon through a natural selection if the proper management of the naturally inhibited area of H. hamabo is not conducted continually.